LOCUS       NZ_CP120081             3167 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog119 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120081
VERSION     NZ_CP120081.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696807
            Assembly: GCF_034024715.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by the database at the submitter's request
REFERENCE   3  (bases 1 to 3167)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 529x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:52:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,752
            Genes (coding)                    :: 2,666
            CDSs (with protein)               :: 2,666
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 45 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 21 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120081.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog119"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            join(2782..3167,1..52)
                     /locus_tag="P3U49_RS14160"
                     /old_locus_tag="P3U49_14160"
     CDS             join(2782..3167,1..52)
                     /locus_tag="P3U49_RS14160"
                     /old_locus_tag="P3U49_14160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_323712952.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSLVGTTKIAVTVSILKF"
                     /name="hypothetical protein"
     gene            complement(563..751)
                     /locus_tag="P3U49_RS14165"
                     /old_locus_tag="P3U49_14165"
     CDS             complement(563..751)
                     /locus_tag="P3U49_RS14165"
                     /old_locus_tag="P3U49_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(1335..2330)
                     /locus_tag="P3U49_RS14170"
                     /old_locus_tag="P3U49_14170"
     CDS             complement(1335..2330)
                     /locus_tag="P3U49_RS14170"
                     /old_locus_tag="P3U49_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     Typing          complement(1101..1265)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          complement(1336..2330)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttggtggga actacaaaaa tagcggtgac tgtttctatt ctgaagtttt aaactcatat
       61 ggagaacttt taactggaaa cataatcaca gcagtttttg aagatgttaa agccaaaaac
      121 ttaaagggcg tagcaaaaaa attagctaaa attgggataa aaggaaacct agctggaatc
      181 gcagctacta tggtttcgaa ggatgtacaa tgtaatctta aatacgggtt actttagata
      241 ctatttattc caattgcttt attgacgttg agcctcggaa cccttaacaa tcccaaaact
      301 tgtcgcatgg tcggcttaat agctcacgct atgccgacat tcgtctgcaa gtttagttaa
      361 gggttcttct caacatcaat aaattttctc ggcataaatg cgtcgtctat tttttattat
      421 cttcatgata atcatatttt tcttccatct cgtcaaaagc atcatcttta tagaaaaaca
      481 aaaagtaagc aacaacacca ataggaataa aaaaacagtg tgaaaacaag aagaaactcg
      541 tttcaaaatc ccctcaaata ttctaattat tctttttgaa attcataacc ttaacaacca
      601 aataaatagc atttattccc atcaaaataa catacggaat taatatatat aaattttcca
      661 aatgagatat aaaaattaat ccaagtaaaa agaaaacatt taaaatagtt gctataacgt
      721 aaccttcatc taacactgtg tttttattca aaataaacac tcctttttta aaaaacttta
      781 tcacaagact tactcaaatt ataaatacct ttattttgag ttttaagggg gtattttaaa
      841 ggtttagggg ggtatcatat aggtttttaa cttaaaaata tatacaagct cttaaaacgc
      901 aaatatgagc caaataaata tatggtaatt tcacaaacaa aaatttcagc aaaaccagtg
      961 actaattttt taacactgcc catttacatg caaattaaaa attagcataa aatcctttaa
     1021 aacttccaca tgtttttcta aattcctgca gggcgttttt tatcaaaaat cgagaatttt
     1081 taatttttct ctaaaaactc cgtaagagca taaggagaat ttgctatcat tcaaaaatga
     1141 taggtctggc aaagccagac attccagggg ttttaaggta ttttaaagtg atatcataca
     1201 aagctgtact gtcttatttt tgtgacacaa tatattgcgt ttgtcttatt tttgtgacac
     1261 tacatacagc atttgtcaca aaaataagac agtacttttt tataaaaaat caacaaaaaa
     1321 gacattagtc aacactaatg tcttaaataa taattttgct tttcgaaatt ccaaattgcg
     1381 gtaattgaat gtgctttttc ttcttcgtct gttgttttat cttcatcact tgcattaatc
     1441 aaattaccat cttcgacatc gtctaagttt aaaattttat gtttttgttt aagcaatcca
     1501 ccataactta acatacgttt tcgatacaaa cctttttcta aatcacttac aatttcttga
     1561 tttttttcat catcatcagt taaaaaatca gatgatttaa ccgaatattt tgaggtctct
     1621 ttgattgccg attcaatgtc tttatcgcct tttctattcg gtttgatcgc tttaacatta
     1681 gcaacaggtc gatagtcaac ttgtaatgct ctttgccata aattaaccca ttcttcttga
     1741 gttatataat tctctttttt tctaaaatat gcattttcaa cacataacaa aacatgcatg
     1801 tgctgattat aactaccgtc atttttatta acggtaactt ctgttgaacg cataaatcca
     1861 acaagatttt gtttaacctt tttatatcta ctcaacctat caaatgcttt agttagatgc
     1921 ttcaaacttt gttctaaagt atctccatct atcgcatttt ttgttgaaag tgttaaaaac
     1981 aaccaacgtg cttttggctt ttccttaatt actttttcaa tcactttttg agcttgataa
     2041 ctatttttca tagcacgtct ccaattacaa accggacata attttgattt acaaaaccat
     2101 gtcttatgta acttcaaata gccttcatca gttggcttaa attctaaaat attaccacat
     2161 tgttttacat taaaagcctt tttgattttt aaaatttcta gtatatctgc atagcttaca
     2221 ttatctattt tcttttctct ccatgggcgt tgtttcccac ttttcgtcat atcttttaac
     2281 gttttattat cttgattttc ggttatactt ctagtagtat tatattgcat ataaaaagac
     2341 ccccagtatt atttaagatt cgacacctta ataatataac tggggttttt tattgtcaaa
     2401 ttttcctttc acgcacaaaa acacgtttaa acactgtaat atcaacagtt acagctatat
     2461 atataaaatc gagttatttc tatatagtat caagacaaga agaaactcgt tcacactcgt
     2521 ttcaaaaaac aacctattcc ttcttctttt tatgaccctc taaacctaaa taaattaatg
     2581 tgaaaaccaa tgaataaata aaactttcta aaaagtcaaa aggattatca aataaaatcg
     2641 aattaacaaa attaaaaatt aaaaatacag ataataaaat aatcaaaaag taaaaaaact
     2701 tattcatatt aaactccttt gtttttatat gttaatatta tattgaattc atttaaataa
     2761 caaaacaagg gggcatttac aatgaatgca attcaaaaca gtatatcact aagaaagatt
     2821 ttagtagtat ctttaagtat tttaaccata ctttcaatgg tcttggattt taatttcaaa
     2881 gaagctcaag cccaaaacaa aaataacatt gcagataaaa acgttgaaac tttaaacgaa
     2941 aaagaaattg aaaaagaact aaaatacata tatggcaaaa ttattatttt agataaagac
     3001 ggaacagcta aagatgttaa tctagaaaat gccaaatctc gatatggata tgttcctgaa
     3061 ggattccaaa aactaaaaaa cgacatagag aataaaaaga aaactagttc aatatcacct
     3121 agagcagttg gtgggaacta caaaaatagc ggtgactgtt tctattc
//
