LOCUS       NZ_CP120072             3126 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog125 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120072
VERSION     NZ_CP120072.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696811
            Assembly: GCF_034043415.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by the database at the submitter's request
REFERENCE   3  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 291x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 11:01:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,833
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,664
            CDSs (with protein)               :: 2,664
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 46 of 87
            Pseudo Genes (incomplete)         :: 35 of 87
            Pseudo Genes (internal stop)      :: 21 of 87
            Pseudo Genes (multiple problems)  :: 15 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120072.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog125"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            complement(23..211)
                     /locus_tag="P3U73_RS14155"
                     /old_locus_tag="P3U73_14155"
     CDS             complement(23..211)
                     /locus_tag="P3U73_RS14155"
                     /old_locus_tag="P3U73_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(795..1790)
                     /locus_tag="P3U73_RS14160"
                     /old_locus_tag="P3U73_14160"
     CDS             complement(795..1790)
                     /locus_tag="P3U73_RS14160"
                     /old_locus_tag="P3U73_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            2242..2823
                     /locus_tag="P3U73_RS14165"
                     /old_locus_tag="P3U73_14165"
     CDS             2242..2823
                     /locus_tag="P3U73_RS14165"
                     /old_locus_tag="P3U73_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          complement(561..725)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          complement(796..1790)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttcaaaatc ccctcaaata ttctaattat tctttttgaa attcataacc ttaacaacca
       61 aataaatagc atttattccc atcaaaataa catacggaat taatatatat aaattttcca
      121 aatgagatat aaaaattaat ccaagtaaaa agaaaacatt taaaatagtt gctataacgt
      181 aaccttcatc taacactgtg tttttattca aaataaacac tcctttttta aaaaacttta
      241 tcacaagact tactcaaatt ataaatacct ttattttgag ttttaagggg gtattttaaa
      301 ggtttagggg ggtatcatat aggtttttaa cttaaaaata tatacaagct cttaaaacgc
      361 aaatatgagc caaataaata tatggtaatt tcacaaacaa aaatttcagc aaaaccagtg
      421 actaattttt taacactgcc catttacatg caaattaaaa attagcataa aatcctttaa
      481 aacttccaca tgtttttcta aattcctgca gggcgttttt tatcaaaaat cgagaatttt
      541 taatttttct ctaaaaactc cgtaagagca taaggagaat ttgctatcat tcaaaaatga
      601 taggtctggc aaagccagac attccagggg ttttaaggta ttttaaagtg atatcataca
      661 aagctgtact gtcttatttt tgtgacacaa tatattgcgt ttgtcttatt tttgtgacac
      721 tacatacagc atttgtcaca aaaataagac agtacttttt tataaaaaat caacaaaaaa
      781 gacattagtc aacactaatg tcttaaataa taattttgct tttcgaaatt ccaaattgcg
      841 gtaattgaat gtgctttttc ttcttcgtct gttgttttat cttcatcact tgcattaatc
      901 aaattaccat cttcgacatc gtctaagttt aaaattttat gtttttgttt aagcaatcca
      961 ccataactta acatacgttt tcgatacaaa cctttttcta aatcacttac aatttcttga
     1021 tttttttcat catcatcagt taaaaaatca gatgatttaa ccgaatattt tgaggtctct
     1081 ttgattgccg attcaatgtc tttatcgcct tttctattcg gtttgatcgc tttaacatta
     1141 gcaacaggtc gatagtcaac ttgtaatgct ctttgccata aattaaccca ttcttcttga
     1201 gttatataat tctctttttt tctaaaatat gcattttcaa cacataacaa aacatgcatg
     1261 tgctgattat aactaccgtc atttttatta acggtaactt ctgttgaacg cataaatcca
     1321 acaagatttt gtttaacctt tttatatcta ctcaacctat caaatgcttt agttagatgc
     1381 ttcaaacttt gttctaaagt atctccatct atcgcatttt ttgttgaaag tgttaaaaac
     1441 aaccaacgtg cttttggctt ttccttaatt actttttcaa tcactttttg agcttgataa
     1501 ctatttttca tagcacgtct ccaattacaa accggacata attttgattt acaaaaccat
     1561 gtcttatgta acttcaaata gccttcatca gttggcttaa attctaaaat attaccacat
     1621 tgttttacat taaaagcctt tttgattttt aaaatttcta gtatatctgc atagcttaca
     1681 ttatctattt tcttttctct ccatgggcgt tgtttcccac ttttcgtcat atcttttaac
     1741 gttttattat cttgattttc ggttatactt ctagtagtat tatattgcat ataaaaagac
     1801 ccccagtatt atttaagatt cgacacctta ataatataac tggggttttt tattgtcaaa
     1861 ttttcctttc acgcacaaaa acacgtttaa acactgtaat atcaacagtt acagctatat
     1921 atataaaatc gagttatttc tatatagtat caagacaaga agaaactcgt tcacactcgt
     1981 ttcaaaaaac aacctattcc ttcttctttt tatgaccctc taaacctaaa taaattaatg
     2041 tgaaaaccaa tgaataaata aaactttcta aaaagtcaaa aggattatca aataaaatcg
     2101 aattaacaaa attaaaaatt aaaaatacag ataataaaat aatcaaaaag taaaaaaact
     2161 tattcatatt aaactccttt gtttttatat gttaatatta tattgaattc atttaaataa
     2221 caaaacaagg gggcatttac aatgaatgca attcaaaaca gtatatcact aagaaagatt
     2281 ttagtagtat ctttaagtat tttaaccata ctttcaatgg tcttggattt taatttcaaa
     2341 gaagctcaag cccaaaacaa aaataacatt gcagataaaa acgttgaaac tttaaacgaa
     2401 aaagaaattg aaaaagaact aaaatacata tatggcaaaa ttattatttt agataaagac
     2461 ggaacagcta aagatgttaa tctagaaaat gccaaatctc gatatggata tgttcctgaa
     2521 ggattccaaa aactaaaaaa cgacatagag aataaaaaga aaactagttc aatatcacct
     2581 agagcagttg gtgggaacta caaaaatagc ggtgactgtt tctattctga agttttaaac
     2641 tcatatggag aacttttaac tggaaacata atcacagcag tttttgaaga tgttaaagcc
     2701 aaaaacttaa agggcgtagc aaaaaaatta gctaaaattg ggataaaagg aaacctagct
     2761 ggaatcgcag ctactatggt ttcgaaggat gtacaatgta atcttaaata cgggttactt
     2821 tagatactat ttattccaat tgctttattg acgttgagcc tcggaaccct taacaatccc
     2881 aaaacttgtc gcatggtcgg cttaatagct cacgctatgc cgacattcgt ctgcaagttt
     2941 agttaagggt tcttctcaac atcaataaat tttctcggca taaatgcgtc gtctattttt
     3001 tattatcttc atgataatca tatttttctt ccatctcgtc aaaagcatca tctttataga
     3061 aaaacaaaaa gtaagcaaca acaccaatag gaataaaaaa acagtgtgaa aacaagaaga
     3121 aactcg
//
