LOCUS       NZ_CP120069             2968 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog126 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120069
VERSION     NZ_CP120069.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696812
            Assembly: GCF_034043395.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by the database at the submitter's request
REFERENCE   3  (bases 1 to 2968)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 328x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:59:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,835
            CDSs (total)                      :: 2,753
            Genes (coding)                    :: 2,667
            CDSs (with protein)               :: 2,667
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 45 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 21 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120069.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog126"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            complement(7..588)
                     /locus_tag="P3U16_RS14165"
                     /old_locus_tag="P3U16_14165"
     CDS             complement(7..588)
                     /locus_tag="P3U16_RS14165"
                     /old_locus_tag="P3U16_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            1040..2035
                     /locus_tag="P3U16_RS14170"
                     /old_locus_tag="P3U16_14170"
     CDS             1040..2035
                     /locus_tag="P3U16_RS14170"
                     /old_locus_tag="P3U16_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            2619..2807
                     /locus_tag="P3U16_RS14175"
                     /old_locus_tag="P3U16_14175"
     CDS             2619..2807
                     /locus_tag="P3U16_RS14175"
                     /old_locus_tag="P3U16_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     Typing          2106..2270
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          1041..2035
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tagtatctaa agtaacccgt atttaagatt acattgtaca tccttcgaaa ccatagtagc
       61 tgcgattcca gctaggtttc cttttatccc aattttagct aatttttttg ctacgccctt
      121 taagtttttg gctttaacat cttcaaaaac tgctgtgatt atgtttccag ttaaaagttc
      181 tccatatgag tttaaaactt cagaatagaa acagtcaccg ctatttttgt agttcccacc
      241 aactgctcta ggtgatattg aactagtttt ctttttattc tctatgtcgt tttttagttt
      301 ttggaatcct tcaggaacat atccatatcg agatttggca ttttctagat taacatcttt
      361 agctgttccg tctttatcta aaataataat tttgccatat atgtatttta gttctttttc
      421 aatttctttt tcgtttaaag tttcaacgtt tttatctgca atgttatttt tgttttgggc
      481 ttgagcttct ttgaaattaa aatccaagac cattgaaagt atggttaaaa tacttaaaga
      541 tactactaaa atctttctta gtgatatact gttttgaatt gcattcattg taaatgcccc
      601 cttgttttgt tatttaaatg aattcaatat aatattaaca tataaaaaca aaggagttta
      661 atatgaataa gtttttttac tttttgatta ttttattatc tgtattttta atttttaatt
      721 ttgttaattc gattttattt gataatcctt ttgacttttt agaaagtttt atttattcat
      781 tggttttcac attaatttat ttaggtttag agggtcataa aaagaagaag gaataggttg
      841 ttttttgaaa cgagtgtgaa cgagtttctt cttgtcttga tactatatag aaataactcg
      901 attttatata tatagctgta actgttgata ttacagtgtt taaacgtgtt tttgtgcgtg
      961 aaaggaaaat ttgacaataa aaaaccccag ttatattatt aaggtgtcga atcttaaata
     1021 atactggggg tctttttata tgcaatataa tactactaga agtataaccg aaaatcaaga
     1081 taataaaacg ttaaaagata tgacgaaaag tgggaaacaa cgcccatgga gagaaaagaa
     1141 aatagataat gtaagctatg cagatatact agaaatttta aaaatcaaaa aggcttttaa
     1201 tgtaaaacaa tgtggtaata ttttagaatt taagccaact gatgaaggct atttgaagtt
     1261 acataagaca tggttttgta aatcaaaatt atgtccggtt tgtaattgga gacgtgctat
     1321 gaaaaatagt tatcaagctc aaaaagtgat tgaaaaagta attaaggaaa agccaaaagc
     1381 acgttggttg tttttaacac tttcaacaaa aaatgcgata gatggagata ctttagaaca
     1441 aagtttgaag catctaacta aagcatttga taggttgagt agatataaaa aggttaaaca
     1501 aaatcttgtt ggatttatgc gttcaacaga agttaccgtt aataaaaatg acggtagtta
     1561 taatcagcac atgcatgttt tgttatgtgt tgaaaatgca tattttagaa aaaaagagaa
     1621 ttatataact caagaagaat gggttaattt atggcaaaga gcattacaag ttgactatcg
     1681 acctgttgct aatgttaaag cgatcaaacc gaatagaaaa ggcgataaag acattgaatc
     1741 ggcaatcaaa gagacctcaa aatattcggt taaatcatct gattttttaa ctgatgatga
     1801 tgaaaaaaat caagaaattg taagtgattt agaaaaaggt ttgtatcgaa aacgtatgtt
     1861 aagttatggt ggattgctta aacaaaaaca taaaatttta aacttagacg atgtcgaaga
     1921 tggtaatttg attaatgcaa gtgatgaaga taaaacaaca gacgaagaag aaaaagcaca
     1981 ttcaattacc gcaatttgga atttcgaaaa gcaaaattat tatttaagac attagtgttg
     2041 actaatgtct tttttgttga ttttttataa aaaagtactg tcttattttt gtgacaaatg
     2101 ctgtatgtag tgtcacaaaa ataagacaaa cgcaatatat tgtgtcacaa aaataagaca
     2161 gtacagcttt gtatgatatc actttaaaat accttaaaac ccctggaatg tctggctttg
     2221 ccagacctat catttttgaa tgatagcaaa ttctccttat gctcttacgg agtttttaga
     2281 gaaaaattaa aaattctcga tttttgataa aaaacgccct gcaggaattt agaaaaacat
     2341 gtggaagttt taaaggattt tatgctaatt tttaatttgc atgtaaatgg gcagtgttaa
     2401 aaaattagtc actggttttg ctgaaatttt tgtttgtgaa attaccatat atttatttgg
     2461 ctcatatttg cgttttaaga gcttgtatat atttttaagt taaaaaccta tatgataccc
     2521 ccctaaacct ttaaaatacc cccttaaaac tcaaaataaa ggtatttata atttgagtaa
     2581 gtcttgtgat aaagtttttt aaaaaaggag tgtttatttt gaataaaaac acagtgttag
     2641 atgaaggtta cgttatagca actattttaa atgttttctt tttacttgga ttaattttta
     2701 tatctcattt ggaaaattta tatatattaa ttccgtatgt tattttgatg ggaataaatg
     2761 ctatttattt ggttgttaag gttatgaatt tcaaaaagaa taattagaat atttgagggg
     2821 attttgaaac gagtttcttc ttgttttcac actgtttttt tattcctatt ggtgttgttg
     2881 cttacttttt gtttttctat aaagatgatg cttttgacga gatggaagaa aaatatgatt
     2941 atcatgaaga taataaaaaa tagacgac
//
