LOCUS       NZ_CP120052             3141 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog132 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120052
VERSION     NZ_CP120052.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696818
            Assembly: GCF_034043315.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the submitter's request
REFERENCE   3  (bases 1 to 3141)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 262x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:59:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,831
            CDSs (total)                      :: 2,749
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 46 of 87
            Pseudo Genes (incomplete)         :: 35 of 87
            Pseudo Genes (internal stop)      :: 21 of 87
            Pseudo Genes (multiple problems)  :: 15 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120052.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog132"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            join(2574..3141,1..8)
                     /locus_tag="P3U28_RS14145"
                     /old_locus_tag="P3U28_14145"
     CDS             join(2574..3141,1..8)
                     /locus_tag="P3U28_RS14145"
                     /old_locus_tag="P3U28_14145"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_323713051.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKSM"
                     /name="hypothetical protein"
     gene            complement(356..544)
                     /locus_tag="P3U28_RS14150"
                     /old_locus_tag="P3U28_14150"
     CDS             complement(356..544)
                     /locus_tag="P3U28_RS14150"
                     /old_locus_tag="P3U28_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(1128..2123)
                     /locus_tag="P3U28_RS14155"
                     /old_locus_tag="P3U28_14155"
     CDS             complement(1128..2123)
                     /locus_tag="P3U28_RS14155"
                     /old_locus_tag="P3U28_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     Typing          complement(894..1058)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          complement(1129..2123)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caatgtaatc ttaaatacgg gttactttag atactattta ttccaattgc tttattgacg
       61 ttgagcctcg gaacccttaa caatcccaaa acttgtcgca tggtcggctt aatagctcac
      121 gctatgccga cattcgtctg caagtttagt taagggttct tctcaacatc aataaatttt
      181 ctcggcataa atgcgtcgtc tattttttat tatcttcatg ataatcatat ttttcttcca
      241 tctcgtcaaa agcatcatct ttatagaaaa acaaaaagta agcaacaaca ccaataggaa
      301 taaaaaaaca gtgtgaaaac aagaagaaac tcgtttcaaa atcccctcaa atattctaat
      361 tattcttttt gaaattcata accttaacaa ccaaataaat agcatttatt cccatcaaaa
      421 taacatacgg aattaatata tataaatttt ccaaatgaga tataaaaatt aatccaagta
      481 aaaagaaaac atttaaaata gttgctataa cgtaaccttc atctaacact gtgtttttat
      541 tcaaaataaa cactcctttt ttaaaaaact ttatcacaag acttactcaa attataaata
      601 cctttatttt gagttttaag ggggtatttt aaaggtttag gggggtatca tataggtttt
      661 taacttaaaa atatatacaa gctcttaaaa cgcaaatatg agccaaataa atatatggta
      721 atttcacaaa caaaaatttc agcaaaacca gtgactaatt ttttaacact gcccatttac
      781 atgcaaatta aaaattagca taaaatcctt taaaacttcc acatgttttt ctaaattcct
      841 gcagggcgtt ttttatcaaa aatcgagaat ttttaatttt tctctaaaaa ctccgtaaga
      901 gcataaggag aatttgctat cattcaaaaa tgataggtct ggcaaagcca gacattccag
      961 gggttttaag gtattttaaa gtgatatcat acaaagctgt actgtcttat ttttgtgaca
     1021 caatatattg cgtttgtctt atttttgtga cactacatac agcatttgtc acaaaaataa
     1081 gacagtactt ttttataaaa aatcaacaaa aaagacatta gtcaacacta atgtcttaaa
     1141 taataatttt gcttttcgaa attccaaatt gcggtaattg aatgtgcttt ttcttcttcg
     1201 tctgttgttt tatcttcatc acttgcatta atcaaattac catcttcgac atcgtctaag
     1261 tttaaaattt tatgtttttg tttaagcaat ccaccataac ttaacatacg ttttcgatac
     1321 aaaccttttt ctaaatcact tacaatttct tgattttttt catcatcatc agttaaaaaa
     1381 tcagatgatt taaccgaata ttttgaggtc tctttgattg ccgattcaat gtctttatcg
     1441 ccttttctat tcggtttgat cgctttaaca ttagcaacag gtcgatagtc aacttgtaat
     1501 gctctttgcc ataaattaac ccattcttct tgagttatat aattctcttt ttttctaaaa
     1561 tatgcatttt caacacataa caaaacatgc atgtgctgat tataactacc gtcattttta
     1621 ttaacggtaa cttctgttga acgcataaat ccaacaagat tttgtttaac ctttttatat
     1681 ctactcaacc tatcaaatgc tttagttaga tgcttcaaac tttgttctaa agtatctcca
     1741 tctatcgcat tttttgttga aagtgttaaa aacaaccaac gtgcttttgg cttttcctta
     1801 attacttttt caatcacttt ttgagcttga taactatttt tcatagcacg tctccaatta
     1861 caaaccggac ataattttga tttacaaaac catgtcttat gtaacttcaa atagccttca
     1921 tcagttggct taaattctaa aatattacca cattgtttta cattaaaagc ctttttgatt
     1981 tttaaaattt ctagtatatc tgcatagctt acattatcta ttttcttttc tctccatggg
     2041 cgttgtttcc cacttttcgt catatctttt aacgttttat tatcttgatt ttcggttata
     2101 cttctagtag tattatattg catataaaaa gacccccagt attatttaag attcgacacc
     2161 ttaataatat aactggggtt ttttattgtc aaattttcct ttcacgcaca aaaacacgtt
     2221 taaacactgt aatatcaaca gttacagcta tatatataaa atcgagttat ttctatatag
     2281 tatcaagaca agaagaaact cgttcacact cgtttcaaaa aacaacctat tccttcttct
     2341 ttttatgacc ctctaaacct aaataaatta atgtgaaaac caatgaataa ataaaacttt
     2401 ctaaaaagtc aaaaggatta tcaaataaaa tcgaattaac aaaattaaaa attaaaaata
     2461 cagataataa aataatcaaa aagtaaaaaa acttattcat attaaactcc tttgttttta
     2521 tatgttaata ttatattgaa ttcatttaaa taacaaaaca aggggcattt acaatgaatg
     2581 caattcaaaa cagtatatca ctaagaaaga ttttagtagt atctttaagt attttaacca
     2641 tactttcaat ggtcttggat tttaatttca aagaagctca agcccaaaac aaaaataaca
     2701 ttgcagataa aaacgttgaa actttaaacg aaaaagaaat tgaaaaagaa ctaaaataca
     2761 tatatggcaa aattattatt ttagataaag acggaacagc taaagatgtt aatctagaaa
     2821 atgccaaatc tcgatatgga tatgttcctg aaggattcca aaaactaaaa aacgacatag
     2881 agaataaaaa gaaaactagt tcaatatcac ctagagcagt tggtgggaac tacaaaaata
     2941 gcggtgactg tttctattct gaagttttaa actcatatgg agaactttta actggaaaca
     3001 taatcacagc agtttttgaa gatgttaaag ccaaaaactt aaagggcgta gcaaaaaaat
     3061 tagctaaaat tgggataaaa ggaaacctag ctggaatcgc agctactatg gtttcgaagg
     3121 atgtacaatg taatcttaaa t
//
