LOCUS       NZ_CP120049             3126 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog133 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120049
VERSION     NZ_CP120049.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696819
            Assembly: GCF_034043295.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the submitter's request
REFERENCE   3  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 328x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 11:00:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,838
            CDSs (total)                      :: 2,756
            Genes (coding)                    :: 2,669
            CDSs (with protein)               :: 2,669
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 46 of 87
            Pseudo Genes (incomplete)         :: 35 of 87
            Pseudo Genes (internal stop)      :: 21 of 87
            Pseudo Genes (multiple problems)  :: 15 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120049.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog133"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            304..885
                     /locus_tag="P3U74_RS14180"
                     /old_locus_tag="P3U74_14180"
     CDS             304..885
                     /locus_tag="P3U74_RS14180"
                     /old_locus_tag="P3U74_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            complement(1211..1399)
                     /locus_tag="P3U74_RS14185"
                     /old_locus_tag="P3U74_14185"
     CDS             complement(1211..1399)
                     /locus_tag="P3U74_RS14185"
                     /old_locus_tag="P3U74_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(1983..2978)
                     /locus_tag="P3U74_RS14190"
                     /old_locus_tag="P3U74_14190"
     CDS             complement(1983..2978)
                     /locus_tag="P3U74_RS14190"
                     /old_locus_tag="P3U74_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     Typing          complement(1749..1913)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          complement(1984..2978)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctatatagt atcaagacaa gaagaaactc gttcacactc gtttcaaaaa acaacctatt
       61 ccttcttctt tttatgaccc tctaaaccta aataaattaa tgtgaaaacc aatgaataaa
      121 taaaactttc taaaaagtca aaaggattat caaataaaat cgaattaaca aaattaaaaa
      181 ttaaaaatac agataataaa ataatcaaaa agtaaaaaaa cttattcata ttaaactcct
      241 ttgtttttat atgttaatat tatattgaat tcatttaaat aacaaaacaa gggggcattt
      301 acaatgaatg caattcaaaa cagtatatca ctaagaaaga ttttagtagt atctttaagt
      361 attttaacca tactttcaat ggtcttggat tttaatttca aagaagctca agcccaaaac
      421 aaaaataaca ttgcagataa aaacgttgaa actttaaacg aaaaagaaat tgaaaaagaa
      481 ctaaaataca tatatggcaa aattattatt ttagataaag acggaacagc taaagatgtt
      541 aatctagaaa atgccaaatc tcgatatgga tatgttcctg aaggattcca aaaactaaaa
      601 aacgacatag agaataaaaa gaaaactagt tcaatatcac ctagagcagt tggtgggaac
      661 tacaaaaata gcggtgactg tttctattct gaagttttaa actcatatgg agaactttta
      721 actggaaaca taatcacagc agtttttgaa gatgttaaag ccaaaaactt aaagggcgta
      781 gcaaaaaaat tagctaaaat tgggataaaa ggaaacctag ctggaatcgc agctactatg
      841 gtttcgaagg atgtacaatg taatcttaaa tacgggttac tttagatact atttattcca
      901 attgctttat tgacgttgag cctcggaacc cttaacaatc ccaaaacttg tcgcatggtc
      961 ggcttaatag ctcacgctat gccgacattc gtctgcaagt ttagttaagg gttcttctca
     1021 acatcaataa attttctcgg cataaatgcg tcgtctattt tttattatct tcatgataat
     1081 catatttttc ttccatctcg tcaaaagcat catctttata gaaaaacaaa aagtaagcaa
     1141 caacaccaat aggaataaaa aaacagtgtg aaaacaagaa gaaactcgtt tcaaaatccc
     1201 ctcaaatatt ctaattattc tttttgaaat tcataacctt aacaaccaaa taaatagcat
     1261 ttattcccat caaaataaca tacggaatta atatatataa attttccaaa tgagatataa
     1321 aaattaatcc aagtaaaaag aaaacattta aaatagttgc tataacgtaa ccttcatcta
     1381 acactgtgtt tttattcaaa ataaacactc cttttttaaa aaactttatc acaagactta
     1441 ctcaaattat aaataccttt attttgagtt ttaagggggt attttaaagg tttagggggg
     1501 tatcatatag gtttttaact taaaaatata tacaagctct taaaacgcaa atatgagcca
     1561 aataaatata tggtaatttc acaaacaaaa atttcagcaa aaccagtgac taatttttta
     1621 acactgccca tttacatgca aattaaaaat tagcataaaa tcctttaaaa cttccacatg
     1681 tttttctaaa ttcctgcagg gcgtttttta tcaaaaatcg agaattttta atttttctct
     1741 aaaaactccg taagagcata aggagaattt gctatcattc aaaaatgata ggtctggcaa
     1801 agccagacat tccaggggtt ttaaggtatt ttaaagtgat atcatacaaa gctgtactgt
     1861 cttatttttg tgacacaata tattgcgttt gtcttatttt tgtgacacta catacagcat
     1921 ttgtcacaaa aataagacag tactttttta taaaaaatca acaaaaaaga cattagtcaa
     1981 cactaatgtc ttaaataata attttgcttt tcgaaattcc aaattgcggt aattgaatgt
     2041 gctttttctt cttcgtctgt tgttttatct tcatcacttg cattaatcaa attaccatct
     2101 tcgacatcgt ctaagtttaa aattttatgt ttttgtttaa gcaatccacc ataacttaac
     2161 atacgttttc gatacaaacc tttttctaaa tcacttacaa tttcttgatt tttttcatca
     2221 tcatcagtta aaaaatcaga tgatttaacc gaatattttg aggtctcttt gattgccgat
     2281 tcaatgtctt tatcgccttt tctattcggt ttgatcgctt taacattagc aacaggtcga
     2341 tagtcaactt gtaatgctct ttgccataaa ttaacccatt cttcttgagt tatataattc
     2401 tctttttttc taaaatatgc attttcaaca cataacaaaa catgcatgtg ctgattataa
     2461 ctaccgtcat ttttattaac ggtaacttct gttgaacgca taaatccaac aagattttgt
     2521 ttaacctttt tatatctact caacctatca aatgctttag ttagatgctt caaactttgt
     2581 tctaaagtat ctccatctat cgcatttttt gttgaaagtg ttaaaaacaa ccaacgtgct
     2641 tttggctttt ccttaattac tttttcaatc actttttgag cttgataact atttttcata
     2701 gcacgtctcc aattacaaac cggacataat tttgatttac aaaaccatgt cttatgtaac
     2761 ttcaaatagc cttcatcagt tggcttaaat tctaaaatat taccacattg ttttacatta
     2821 aaagcctttt tgatttttaa aatttctagt atatctgcat agcttacatt atctattttc
     2881 ttttctctcc atgggcgttg tttcccactt ttcgtcatat cttttaacgt tttattatct
     2941 tgattttcgg ttatacttct agtagtatta tattgcatat aaaaagaccc ccagtattat
     3001 ttaagattcg acaccttaat aatataactg gggtttttta ttgtcaaatt ttcctttcac
     3061 gcacaaaaac acgtttaaac actgtaatat caacagttac agctatatat ataaaatcga
     3121 gttatt
//
