LOCUS       NZ_CP120046             3126 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog135 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120046
VERSION     NZ_CP120046.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696820
            Assembly: GCF_034043275.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the submitter's request
REFERENCE   3  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 802x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 11:04:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,863
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 46 of 87
            Pseudo Genes (incomplete)         :: 35 of 87
            Pseudo Genes (internal stop)      :: 21 of 87
            Pseudo Genes (multiple problems)  :: 15 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120046.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog135"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            complement(join(2877..3126,1..332))
                     /locus_tag="P3U65_RS14305"
                     /old_locus_tag="P3U65_14305"
     CDS             complement(join(2877..3126,1..332))
                     /locus_tag="P3U65_RS14305"
                     /old_locus_tag="P3U65_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            784..1779
                     /locus_tag="P3U65_RS14310"
                     /old_locus_tag="P3U65_14310"
     CDS             784..1779
                     /locus_tag="P3U65_RS14310"
                     /old_locus_tag="P3U65_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            2363..2551
                     /locus_tag="P3U65_RS14315"
                     /old_locus_tag="P3U65_14315"
     CDS             2363..2551
                     /locus_tag="P3U65_RS14315"
                     /old_locus_tag="P3U65_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     Typing          1850..2014
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          785..1779
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attgaactag ttttcttttt attctctatg tcgtttttta gtttttggaa tccttcagga
       61 acatatccat atcgagattt ggcattttct agattaacat ctttagctgt tccgtcttta
      121 tctaaaataa taattttgcc atatatgtat tttagttctt tttcaatttc tttttcgttt
      181 aaagtttcaa cgtttttatc tgcaatgtta tttttgtttt gggcttgagc ttctttgaaa
      241 ttaaaatcca agaccattga aagtatggtt aaaatactta aagatactac taaaatcttt
      301 cttagtgata tactgttttg aattgcattc attgtaaatg cccccttgtt ttgttattta
      361 aatgaattca atataatatt aacatataaa aacaaaggag tttaatatga ataagttttt
      421 ttactttttg attattttat tatctgtatt tttaattttt aattttgtta attcgatttt
      481 atttgataat ccttttgact ttttagaaag ttttatttat tcattggttt tcacattaat
      541 ttatttaggt ttagagggtc ataaaaagaa gaaggaatag gttgtttttt gaaacgagtg
      601 tgaacgagtt tcttcttgtc ttgatactat atagaaataa ctcgatttta tatatatagc
      661 tgtaactgtt gatattacag tgtttaaacg tgtttttgtg cgtgaaagga aaatttgaca
      721 ataaaaaacc ccagttatat tattaaggtg tcgaatctta aataatactg ggggtctttt
      781 tatatgcaat ataatactac tagaagtata accgaaaatc aagataataa aacgttaaaa
      841 gatatgacga aaagtgggaa acaacgccca tggagagaaa agaaaataga taatgtaagc
      901 tatgcagata tactagaaat tttaaaaatc aaaaaggctt ttaatgtaaa acaatgtggt
      961 aatattttag aatttaagcc aactgatgaa ggctatttga agttacataa gacatggttt
     1021 tgtaaatcaa aattatgtcc ggtttgtaat tggagacgtg ctatgaaaaa tagttatcaa
     1081 gctcaaaaag tgattgaaaa agtaattaag gaaaagccaa aagcacgttg gttgttttta
     1141 acactttcaa caaaaaatgc gatagatgga gatactttag aacaaagttt gaagcatcta
     1201 actaaagcat ttgataggtt gagtagatat aaaaaggtta aacaaaatct tgttggattt
     1261 atgcgttcaa cagaagttac cgttaataaa aatgacggta gttataatca gcacatgcat
     1321 gttttgttat gtgttgaaaa tgcatatttt agaaaaaaag agaattatat aactcaagaa
     1381 gaatgggtta atttatggca aagagcatta caagttgact atcgacctgt tgctaatgtt
     1441 aaagcgatca aaccgaatag aaaaggcgat aaagacattg aatcggcaat caaagagacc
     1501 tcaaaatatt cggttaaatc atctgatttt ttaactgatg atgatgaaaa aaatcaagaa
     1561 attgtaagtg atttagaaaa aggtttgtat cgaaaacgta tgttaagtta tggtggattg
     1621 cttaaacaaa aacataaaat tttaaactta gacgatgtcg aagatggtaa tttgattaat
     1681 gcaagtgatg aagataaaac aacagacgaa gaagaaaaag cacattcaat taccgcaatt
     1741 tggaatttcg aaaagcaaaa ttattattta agacattagt gttgactaat gtcttttttg
     1801 ttgatttttt ataaaaaagt actgtcttat ttttgtgaca aatgctgtat gtagtgtcac
     1861 aaaaataaga caaacgcaat atattgtgtc acaaaaataa gacagtacag ctttgtatga
     1921 tatcacttta aaatacctta aaacccctgg aatgtctggc tttgccagac ctatcatttt
     1981 tgaatgatag caaattctcc ttatgctctt acggagtttt tagagaaaaa ttaaaaattc
     2041 tcgatttttg ataaaaaacg ccctgcagga atttagaaaa acatgtggaa gttttaaagg
     2101 attttatgct aatttttaat ttgcatgtaa atgggcagtg ttaaaaaatt agtcactggt
     2161 tttgctgaaa tttttgtttg tgaaattacc atatatttat ttggctcata tttgcgtttt
     2221 aagagcttgt atatattttt aagttaaaaa cctatatgat acccccctaa acctttaaaa
     2281 taccccctta aaactcaaaa taaaggtatt tataatttga gtaagtcttg tgataaagtt
     2341 ttttaaaaaa ggagtgttta ttttgaataa aaacacagtg ttagatgaag gttacgttat
     2401 agcaactatt ttaaatgttt tctttttact tggattaatt tttatatctc atttggaaaa
     2461 tttatatata ttaattccgt atgttatttt gatgggaata aatgctattt atttggttgt
     2521 taaggttatg aatttcaaaa agaataatta gaatatttga ggggattttg aaacgagttt
     2581 cttcttgttt tcacactgtt tttttattcc tattggtgtt gttgcttact ttttgttttt
     2641 ctataaagat gatgcttttg acgagatgga agaaaaatat gattatcatg aagataataa
     2701 aaaatagacg acgcatttat gccgagaaaa tttattgatg ttgagaagaa cccttaacta
     2761 aacttgcaga cgaatgtcgg catagcgtga gctattaagc cgaccatgcg acaagttttg
     2821 ggattgttaa gggttccgag gctcaacgtc aataaagcaa ttggaataaa tagtatctaa
     2881 agtaacccgt atttaagatt acattgtaca tccttcgaaa ccatagtagc tgcgattcca
     2941 gctaggtttc cttttatccc aattttagct aatttttttg ctacgccctt taagtttttg
     3001 gctttaacat cttcaaaaac tgctgtgatt atgtttccag ttaaaagttc tccatatgag
     3061 tttaaaactt cagaatagaa acagtcaccg ctatttttgt agttcccacc aactgctcta
     3121 ggtgat
//
