LOCUS       NZ_CP120043             3126 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog136 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120043
VERSION     NZ_CP120043.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696821
            Assembly: GCF_034043255.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the submitter's request
REFERENCE   3  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 281x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 11:06:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,833
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,665
            CDSs (with protein)               :: 2,665
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 45 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 21 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120043.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog136"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            389..577
                     /locus_tag="P3U04_RS14155"
                     /old_locus_tag="P3U04_14155"
     CDS             389..577
                     /locus_tag="P3U04_RS14155"
                     /old_locus_tag="P3U04_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(903..1484)
                     /locus_tag="P3U04_RS14160"
                     /old_locus_tag="P3U04_14160"
     CDS             complement(903..1484)
                     /locus_tag="P3U04_RS14160"
                     /old_locus_tag="P3U04_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            1936..2931
                     /locus_tag="P3U04_RS14165"
                     /old_locus_tag="P3U04_14165"
     CDS             1936..2931
                     /locus_tag="P3U04_RS14165"
                     /old_locus_tag="P3U04_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     Typing          3002..3126
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.160000000000001e-59"
     Typing          1937..2931
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catttttgaa tgatagcaaa ttctccttat gctcttacgg agtttttaga gaaaaattaa
       61 aaattctcga tttttgataa aaaacgccct gcaggaattt agaaaaacat gtggaagttt
      121 taaaggattt tatgctaatt tttaatttgc atgtaaatgg gcagtgttaa aaaattagtc
      181 actggttttg ctgaaatttt tgtttgtgaa attaccatat atttatttgg ctcatatttg
      241 cgttttaaga gcttgtatat atttttaagt taaaaaccta tatgataccc ccctaaacct
      301 ttaaaatacc cccttaaaac tcaaaataaa ggtatttata atttgagtaa gtcttgtgat
      361 aaagtttttt aaaaaaggag tgtttatttt gaataaaaac acagtgttag atgaaggtta
      421 cgttatagca actattttaa atgttttctt tttacttgga ttaattttta tatctcattt
      481 ggaaaattta tatatattaa ttccgtatgt tattttgatg ggaataaatg ctatttattt
      541 ggttgttaag gttatgaatt tcaaaaagaa taattagaat atttgagggg attttgaaac
      601 gagtttcttc ttgttttcac actgtttttt tattcctatt ggtgttgttg cttacttttt
      661 gtttttctat aaagatgatg cttttgacga gatggaagaa aaatatgatt atcatgaaga
      721 taataaaaaa tagacgacgc atttatgccg agaaaattta ttgatgttga gaagaaccct
      781 taactaaact tgcagacgaa tgtcggcata gcgtgagcta ttaagccgac catgcgacaa
      841 gttttgggat tgttaagggt tccgaggctc aacgtcaata aagcaattgg aataaatagt
      901 atctaaagta acccgtattt aagattacat tgtacatcct tcgaaaccat agtagctgcg
      961 attccagcta ggtttccttt tatcccaatt ttagctaatt tttttgctac gccctttaag
     1021 tttttggctt taacatcttc aaaaactgct gtgattatgt ttccagttaa aagttctcca
     1081 tatgagttta aaacttcaga atagaaacag tcaccgctat ttttgtagtt cccaccaact
     1141 gctctaggtg atattgaact agttttcttt ttattctcta tgtcgttttt tagtttttgg
     1201 aatccttcag gaacatatcc atatcgagat ttggcatttt ctagattaac atctttagct
     1261 gttccgtctt tatctaaaat aataattttg ccatatatgt attttagttc tttttcaatt
     1321 tctttttcgt ttaaagtttc aacgttttta tctgcaatgt tatttttgtt ttgggcttga
     1381 gcttctttga aattaaaatc caagaccatt gaaagtatgg ttaaaatact taaagatact
     1441 actaaaatct ttcttagtga tatactgttt tgaattgcat tcattgtaaa tgcccccttg
     1501 ttttgttatt taaatgaatt caatataata ttaacatata aaaacaaagg agtttaatat
     1561 gaataagttt ttttactttt tgattatttt attatctgta tttttaattt ttaattttgt
     1621 taattcgatt ttatttgata atccttttga ctttttagaa agttttattt attcattggt
     1681 tttcacatta atttatttag gtttagaggg tcataaaaag aagaaggaat aggttgtttt
     1741 ttgaaacgag tgtgaacgag tttcttcttg tcttgatact atatagaaat aactcgattt
     1801 tatatatata gctgtaactg ttgatattac agtgtttaaa cgtgtttttg tgcgtgaaag
     1861 gaaaatttga caataaaaaa ccccagttat attattaagg tgtcgaatct taaataatac
     1921 tgggggtctt tttatatgca atataatact actagaagta taaccgaaaa tcaagataat
     1981 aaaacgttaa aagatatgac gaaaagtggg aaacaacgcc catggagaga aaagaaaata
     2041 gataatgtaa gctatgcaga tatactagaa attttaaaaa tcaaaaaggc ttttaatgta
     2101 aaacaatgtg gtaatatttt agaatttaag ccaactgatg aaggctattt gaagttacat
     2161 aagacatggt tttgtaaatc aaaattatgt ccggtttgta attggagacg tgctatgaaa
     2221 aatagttatc aagctcaaaa agtgattgaa aaagtaatta aggaaaagcc aaaagcacgt
     2281 tggttgtttt taacactttc aacaaaaaat gcgatagatg gagatacttt agaacaaagt
     2341 ttgaagcatc taactaaagc atttgatagg ttgagtagat ataaaaaggt taaacaaaat
     2401 cttgttggat ttatgcgttc aacagaagtt accgttaata aaaatgacgg tagttataat
     2461 cagcacatgc atgttttgtt atgtgttgaa aatgcatatt ttagaaaaaa agagaattat
     2521 ataactcaag aagaatgggt taatttatgg caaagagcat tacaagttga ctatcgacct
     2581 gttgctaatg ttaaagcgat caaaccgaat agaaaaggcg ataaagacat tgaatcggca
     2641 atcaaagaga cctcaaaata ttcggttaaa tcatctgatt ttttaactga tgatgatgaa
     2701 aaaaatcaag aaattgtaag tgatttagaa aaaggtttgt atcgaaaacg tatgttaagt
     2761 tatggtggat tgcttaaaca aaaacataaa attttaaact tagacgatgt cgaagatggt
     2821 aatttgatta atgcaagtga tgaagataaa acaacagacg aagaagaaaa agcacattca
     2881 attaccgcaa tttggaattt cgaaaagcaa aattattatt taagacatta gtgttgacta
     2941 atgtcttttt tgttgatttt ttataaaaaa gtactgtctt atttttgtga caaatgctgt
     3001 atgtagtgtc acaaaaataa gacaaacgca atatattgtg tcacaaaaat aagacagtac
     3061 agctttgtat gatatcactt taaaatacct taaaacccct ggaatgtctg gctttgccag
     3121 acctat
//
