LOCUS       NZ_CP120032             3126 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog140 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120032
VERSION     NZ_CP120032.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696825
            Assembly: GCF_034043175.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the submitter's request
REFERENCE   3  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 779x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 11:03:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,862
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,693
            CDSs (with protein)               :: 2,693
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 46 of 87
            Pseudo Genes (incomplete)         :: 35 of 87
            Pseudo Genes (internal stop)      :: 21 of 87
            Pseudo Genes (multiple problems)  :: 15 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog140"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            356..1351
                     /locus_tag="P3U03_RS14295"
                     /old_locus_tag="P3U03_14295"
     CDS             356..1351
                     /locus_tag="P3U03_RS14295"
                     /old_locus_tag="P3U03_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            1935..2123
                     /locus_tag="P3U03_RS14300"
                     /old_locus_tag="P3U03_14300"
     CDS             1935..2123
                     /locus_tag="P3U03_RS14300"
                     /old_locus_tag="P3U03_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            2157..2279
                     /locus_tag="P3U03_RS14305"
                     /old_locus_tag="P3U03_14305"
     CDS             2157..2279
                     /locus_tag="P3U03_RS14305"
                     /old_locus_tag="P3U03_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000491229.1"
                     /translation="MFSHCFFIPIGVVAYFLFFYKDDAFDEMEEKYDYHEDNKK"
                     /name="hypothetical protein"
     gene            complement(2449..3030)
                     /locus_tag="P3U03_RS14310"
                     /old_locus_tag="P3U03_14310"
     CDS             complement(2449..3030)
                     /locus_tag="P3U03_RS14310"
                     /old_locus_tag="P3U03_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          1422..1586
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          357..1351
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgattatttt attatctgta tttttaattt ttaattttgt taattcgatt ttatttgata
       61 atccttttga ctttttagaa agttttattt attcattggt tttcacatta atttatttag
      121 gtttagaggg tcataaaaag aagaaggaat aggttgtttt ttgaaacgag tgtgaacgag
      181 tttcttcttg tcttgatact atatagaaat aactcgattt tatatatata gctgtaactg
      241 ttgatattac agtgtttaaa cgtgtttttg tgcgtgaaag gaaaatttga caataaaaaa
      301 ccccagttat attattaagg tgtcgaatct taaataatac tgggggtctt tttatatgca
      361 atataatact actagaagta taaccgaaaa tcaagataat aaaacgttaa aagatatgac
      421 gaaaagtggg aaacaacgcc catggagaga aaagaaaata gataatgtaa gctatgcaga
      481 tatactagaa attttaaaaa tcaaaaaggc ttttaatgta aaacaatgtg gtaatatttt
      541 agaatttaag ccaactgatg aaggctattt gaagttacat aagacatggt tttgtaaatc
      601 aaaattatgt ccggtttgta attggagacg tgctatgaaa aatagttatc aagctcaaaa
      661 agtgattgaa aaagtaatta aggaaaagcc aaaagcacgt tggttgtttt taacactttc
      721 aacaaaaaat gcgatagatg gagatacttt agaacaaagt ttgaagcatc taactaaagc
      781 atttgatagg ttgagtagat ataaaaaggt taaacaaaat cttgttggat ttatgcgttc
      841 aacagaagtt accgttaata aaaatgacgg tagttataat cagcacatgc atgttttgtt
      901 atgtgttgaa aatgcatatt ttagaaaaaa agagaattat ataactcaag aagaatgggt
      961 taatttatgg caaagagcat tacaagttga ctatcgacct gttgctaatg ttaaagcgat
     1021 caaaccgaat agaaaaggcg ataaagacat tgaatcggca atcaaagaga cctcaaaata
     1081 ttcggttaaa tcatctgatt ttttaactga tgatgatgaa aaaaatcaag aaattgtaag
     1141 tgatttagaa aaaggtttgt atcgaaaacg tatgttaagt tatggtggat tgcttaaaca
     1201 aaaacataaa attttaaact tagacgatgt cgaagatggt aatttgatta atgcaagtga
     1261 tgaagataaa acaacagacg aagaagaaaa agcacattca attaccgcaa tttggaattt
     1321 cgaaaagcaa aattattatt taagacatta gtgttgacta atgtcttttt tgttgatttt
     1381 ttataaaaaa gtactgtctt atttttgtga caaatgctgt atgtagtgtc acaaaaataa
     1441 gacaaacgca atatattgtg tcacaaaaat aagacagtac agctttgtat gatatcactt
     1501 taaaatacct taaaacccct ggaatgtctg gctttgccag acctatcatt tttgaatgat
     1561 agcaaattct ccttatgctc ttacggagtt tttagagaaa aattaaaaat tctcgatttt
     1621 tgataaaaaa cgccctgcag gaatttagaa aaacatgtgg aagttttaaa ggattttatg
     1681 ctaattttta atttgcatgt aaatgggcag tgttaaaaaa ttagtcactg gttttgctga
     1741 aatttttgtt tgtgaaatta ccatatattt atttggctca tatttgcgtt ttaagagctt
     1801 gtatatattt ttaagttaaa aacctatatg atacccccct aaacctttaa aataccccct
     1861 taaaactcaa aataaaggta tttataattt gagtaagtct tgtgataaag ttttttaaaa
     1921 aaggagtgtt tattttgaat aaaaacacag tgttagatga aggttacgtt atagcaacta
     1981 ttttaaatgt tttcttttta cttggattaa tttttatatc tcatttggaa aatttatata
     2041 tattaattcc gtatgttatt ttgatgggaa taaatgctat ttatttggtt gttaaggtta
     2101 tgaatttcaa aaagaataat tagaatattt gaggggattt tgaaacgagt ttcttcttgt
     2161 tttcacactg tttttttatt cctattggtg ttgttgctta ctttttgttt ttctataaag
     2221 atgatgcttt tgacgagatg gaagaaaaat atgattatca tgaagataat aaaaaataga
     2281 cgacgcattt atgccgagaa aatttattga tgttgagaag aacccttaac taaacttgca
     2341 gacgaatgtc ggcatagcgt gagctattaa gccgaccatg cgacaagttt tgggattgtt
     2401 aagggttccg aggctcaacg tcaataaagc aattggaata aatagtatct aaagtaaccc
     2461 gtatttaaga ttacattgta catccttcga aaccatagta gctgcgattc cagctaggtt
     2521 tccttttatc ccaattttag ctaatttttt tgctacgccc tttaagtttt tggctttaac
     2581 atcttcaaaa actgctgtga ttatgtttcc agttaaaagt tctccatatg agtttaaaac
     2641 ttcagaatag aaacagtcac cgctattttt gtagttccca ccaactgctc taggtgatat
     2701 tgaactagtt ttctttttat tctctatgtc gttttttagt ttttggaatc cttcaggaac
     2761 atatccatat cgagatttgg cattttctag attaacatct ttagctgttc cgtctttatc
     2821 taaaataata attttgccat atatgtattt tagttctttt tcaatttctt tttcgtttaa
     2881 agtttcaacg tttttatctg caatgttatt tttgttttgg gcttgagctt ctttgaaatt
     2941 aaaatccaag accattgaaa gtatggttaa aatacttaaa gatactacta aaatctttct
     3001 tagtgatata ctgttttgaa ttgcattcat tgtaaatgcc cccttgtttt gttatttaaa
     3061 tgaattcaat ataatattaa catataaaaa caaaggagtt taatatgaat aagttttttt
     3121 actttt
//
