LOCUS       NZ_CP120022             2395 bp    DNA     circular CON 24-DEC-2023
DEFINITION  Staphylococcus aureus strain Dog148 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120022
VERSION     NZ_CP120022.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696830
            Assembly: GCF_034043075.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the submitter's request
REFERENCE   3  (bases 1 to 2395)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 650x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/24/2023 14:47:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,654
            Genes (coding)                    :: 2,590
            CDSs (with protein)               :: 2,590
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 32 of 64
            Pseudo Genes (incomplete)         :: 32 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 17 of 64
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog148"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            37..480
                     /locus_tag="P3U71_RS13670"
                     /old_locus_tag="P3U71_13670"
     CDS             37..480
                     /locus_tag="P3U71_RS13670"
                     /old_locus_tag="P3U71_13670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012816707.1"
                     /translation="MYKKILTLSILLVMIFTVVPVHNEVEAKPKKGSVVTINKKQYIYQ
                     GTVKKYYPAKYCKLVNKYAKNSQSFTNLVAGTSGFATFAKKAGYAGVLASSLYIANLGV
                     QNNTTIYKNAARKGTGVQVSYDLYVPKTGHNYGLTRNTKVVYK"
                     /name="hypothetical protein"
     gene            780..1706
                     /locus_tag="P3U71_RS13675"
                     /old_locus_tag="P3U71_13675"
     CDS             780..1706
                     /locus_tag="P3U71_RS13675"
                     /old_locus_tag="P3U71_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_031771843.1"
                     /translation="MERYESKKVRSEEFRDLVKSLVSDSYYWLIEECGTFLSFVGDKEL
                     NKIKMHRGNFCKHRFCPMCAWRKARKDALELSLMMQDIKISYDKAFIFLTLTTPNVVAE
                     RLGDEIKRYNKSFDKLFKRKVVKRVVKGYVRKLEITYNEKRDDYHPHFHVLIVVNKSYF
                     TDVKAYLNQKEWLRLWREATGISEITQVDVRRVKEQNSNELYEMAKYTGKDSDYLSNEK
                     VFKVFYESLKGKRAFAYGGLFKEVRKKLKNGDLDYLKEVDPTKYIYQIFCGWNQMHYYA
                     SELRLMSEEMQKEVNHQMINELDDKYL"
                     /name="protein rep"
     gene            2202..2384
                     /locus_tag="P3U71_RS13680"
                     /old_locus_tag="P3U71_13680"
     CDS             2202..2384
                     /locus_tag="P3U71_RS13680"
                     /old_locus_tag="P3U71_13680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000877306.1"
                     /translation="MKYRIIAALFFLMLLLIYVFKIAPFLNTDSQIVNLVVVLIIFFIG
                     ALLGWISRKFDKNSK"
                     /name="hypothetical protein"
     Typing          742..1706
                     /name="rep_cluster_1130"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttatattaat acaaaaatat attaggggtg tattttatgt ataaaaaaat attaacttta
       61 tctattttgc ttgttatgat ttttacagtt gttcctgttc ataatgaagt tgaagctaaa
      121 cctaaaaaag gttctgttgt aacgattaat aaaaagcaat atatatatca agggactgta
      181 aaaaaatatt atcctgcaaa atattgtaaa ctggttaata agtatgctaa gaattcccag
      241 tcttttacaa atttagtggc tggtactagc ggttttgcga cttttgcaaa aaaagctggt
      301 tatgctggtg tgttagcttc gagtttatat attgctaatt taggtgttca gaataatact
      361 acgatttaca aaaatgctgc aagaaaaggt acgggtgtgc aagttagtta tgatttatat
      421 gttcctaaaa caggacataa ttatggatta acacgtaata caaaagttgt atataaataa
      481 aaaaatggag atgtttttca tctccatttt tttattttct tctatcttga taatataggt
      541 aacaactcac ggatgtctgt ataggcatct aacccttgat aataaaggaa aaaacataga
      601 aaaacttgct ttatccagca tattattcta gaattttagg tgtctaatca aaaattctaa
      661 atattggagg tataaagcaa gttttttgtt cgtaatattt ttgtatccaa atttatagcg
      721 tcttaaaatt tgttaattac atgacacata ataatataaa caatcgtaaa atacaagata
      781 ttgagagata tgagagtaaa aaggttagaa gtgaagagtt tagagattta gtaaaaagtt
      841 tagtatctga ttcgtattat tggttaattg aggaatgtgg gacattctta tcgtttgttg
      901 gtgataaaga attaaacaaa attaaaatgc atcgtgggaa tttttgtaag catagatttt
      961 gtccaatgtg tgcatggaga aaagcgagaa aagatgcttt ggaattatct ttaatgatgc
     1021 aggatataaa gattagttat gataaagcat ttatcttttt gacgcttaca acaccgaacg
     1081 tagtagctga aagattggga gatgagatta aaagatataa taaatctttt gataaattat
     1141 ttaaacgaaa agttgtaaaa agagtagtaa aaggttatgt tcgaaaatta gaaataactt
     1201 ataatgagaa acgagatgat tatcacccac attttcatgt gcttattgtt gttaataaat
     1261 catattttac ggatgtaaaa gcttatttga atcaaaaaga gtggttgcgt ttatggcgtg
     1321 aggcaacagg aatttcagaa attacgcaag tagatgtgcg tcgggtcaaa gagcaaaata
     1381 gtaatgagtt gtatgaaatg gctaaatata caggtaaaga tagtgattat ttatctaatg
     1441 aaaaagtgtt taaagttttt tatgagtctt taaaaggtaa aagggcattt gcatatggtg
     1501 gattatttaa agaagttaga aaaaagctta aaaatggcga tttagattat ttaaaagagg
     1561 ttgatccaac aaaatacata tatcaaattt tttgtggttg gaatcaaatg cattattatg
     1621 cgagtgaatt gagattgatg agtgaagaaa tgcaaaaaga agtgaatcat caaatgataa
     1681 atgaattaga tgataaatat ttataagagc tttatttttc aattttatat ataaagaatt
     1741 gcatatgtaa tttagtgtag tctgaggtgc tgacagagtc agcaaggcgg agccgtccag
     1801 cggagcgttc agacaagtaa tataaaatgt gtaggcggag ttttagaatt aagtaaaaga
     1861 atttgttttt gaatgtattt aaagccatta aaaagtatta aactaatttt ggacgtataa
     1921 atgtatcgat tagttatttt gttttaaaat atgcttaaaa tgcgaaataa tcatatattc
     1981 acatttatgc cgagaaaaca ttttgaaaaa gaaaaaagct atatgatatt tcaacttgtt
     2041 gaaaatgttg gtggggtttg aattgggatt gattccaaga aaacaccaac agaaaatcat
     2101 atagcttaaa gtttctgttc aaaatggaaa ttggaataaa tttatggttt attgtataag
     2161 aatgaatata gaatttatta agaaagatta ggaggattta tatgaaatat agaattatag
     2221 cggctttgtt ttttttgatg ttgttattaa tatatgtatt taaaatagct ccttttttaa
     2281 atactgatag tcaaattgta aatttagttg ttgtgttaat tattttcttt ataggtgcat
     2341 tattagggtg gattagtcgt aaatttgata aaaactccaa ataaatatat ttttg
//
