LOCUS       NZ_CP120021            20730 bp    DNA     circular CON 24-DEC-2023
DEFINITION  Staphylococcus aureus strain Dog148 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP120021
VERSION     NZ_CP120021.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696830
            Assembly: GCF_034043075.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20730)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20730)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the submitter's request
REFERENCE   3  (bases 1 to 20730)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 650x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/24/2023 14:47:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,736
            CDSs (total)                      :: 2,654
            Genes (coding)                    :: 2,590
            CDSs (with protein)               :: 2,590
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 32 of 64
            Pseudo Genes (incomplete)         :: 32 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 17 of 64
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120021.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20730
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog148"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            complement(join(20540..20730,1..436))
                     /locus_tag="P3U71_RS13545"
                     /old_locus_tag="P3U71_13545"
     CDS             complement(join(20540..20730,1..436))
                     /locus_tag="P3U71_RS13545"
                     /old_locus_tag="P3U71_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000593103.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN
                     IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN
                     KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD
                     TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(448..759)
                     /locus_tag="P3U71_RS13550"
                     /old_locus_tag="P3U71_13550"
     CDS             complement(448..759)
                     /locus_tag="P3U71_RS13550"
                     /old_locus_tag="P3U71_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="WP_000831610.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFLK"
                     /name="YxeA family protein"
     gene            complement(763..2697)
                     /locus_tag="P3U71_RS13555"
                     /old_locus_tag="P3U71_13555"
     CDS             complement(763..2697)
                     /locus_tag="P3U71_RS13555"
                     /old_locus_tag="P3U71_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000713729.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPS
                     DLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFI
                     FLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANIL
                     IIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSI
                     FMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEK
                     QILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHD
                     FKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFD
                     RTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEIS
                     GITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIH
                     GYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEK
                     YFKDLMREI"
                     /name="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(2772..3065)
                     /locus_tag="P3U71_RS13560"
                     /old_locus_tag="P3U71_13560"
     CDS             complement(2772..3065)
                     /locus_tag="P3U71_RS13560"
                     /old_locus_tag="P3U71_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000726502.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            complement(3479..4087)
                     /locus_tag="P3U71_RS13565"
                     /old_locus_tag="P3U71_13565"
     CDS             complement(3479..4087)
                     /locus_tag="P3U71_RS13565"
                     /old_locus_tag="P3U71_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436987.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001566953.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRPV
                     LQEALNFLRMGDRFVVESIDCLGRNYDEVIHTVNYLKDKEVQLMITSLPMMNEVVGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRI
                     IYHRVVEMLEEGQSISKIAKEVNITRQTIYRIKNDNDLI"
                     /name="recombinase family protein"
     gene            complement(4456..5301)
                     /gene="blaZ"
                     /locus_tag="P3U71_RS13570"
                     /old_locus_tag="P3U71_13570"
     ARG             complement(4460..5301)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5408..7165
                     /gene="blaR1"
                     /locus_tag="P3U71_RS13575"
                     /old_locus_tag="P3U71_13575"
     ARG             5409..7162
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.56"
                     /coverage="100.0"
     gene            7155..7535
                     /gene="blaI"
                     /locus_tag="P3U71_RS13580"
                     /old_locus_tag="P3U71_13580"
     ARG             7156..7532
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            7799..8392
                     /locus_tag="P3U71_RS13585"
                     /old_locus_tag="P3U71_13585"
     CDS             7799..8392
                     /locus_tag="P3U71_RS13585"
                     /old_locus_tag="P3U71_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690638.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            8364..9807
                     /locus_tag="P3U71_RS13590"
                     /old_locus_tag="P3U71_13590"
                     /pseudo=""
     CDS             8364..9807
                     /locus_tag="P3U71_RS13590"
                     /old_locus_tag="P3U71_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /name="DDE-type integrase/transposase/recombinase"
     gene            9800..10615
                     /locus_tag="P3U71_RS13595"
                     /old_locus_tag="P3U71_13595"
     CDS             9800..10615
                     /locus_tag="P3U71_RS13595"
                     /old_locus_tag="P3U71_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000164958.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR
                     YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL
                     GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG
                     MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDYEAIT
                     TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
                     /name="AAA family ATPase"
     gene            complement(10735..11709)
                     /locus_tag="P3U71_RS13600"
                     /old_locus_tag="P3U71_13600"
     CDS             complement(10735..11709)
                     /locus_tag="P3U71_RS13600"
                     /old_locus_tag="P3U71_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WP_000072391.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS
                     SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN
                     KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENSGWI
                     RVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDIEN
                     YLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKL
                     LQKNYKTTLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
                     /name="DUF4062 domain-containing protein"
     gene            complement(11921..12013)
                     /locus_tag="P3U71_RS13605"
                     /old_locus_tag="P3U71_13605"
     CDS             complement(11921..12013)
                     /locus_tag="P3U71_RS13605"
                     /old_locus_tag="P3U71_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(12182..12529)
                     /locus_tag="P3U71_RS13610"
                     /old_locus_tag="P3U71_13610"
     CDS             complement(12182..12529)
                     /locus_tag="P3U71_RS13610"
                     /old_locus_tag="P3U71_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119691.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(12548..13165)
                     /gene="cadD"
                     /locus_tag="P3U71_RS13615"
                     /old_locus_tag="P3U71_13615"
     ARG             complement(12552..13165)
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="99.51"
                     /coverage="100.0"
     gene            complement(14376..>14658)
                     /locus_tag="P3U71_RS13620"
                     /old_locus_tag="P3U71_13620"
                     /pseudo=""
     CDS             complement(14376..>14658)
                     /locus_tag="P3U71_RS13620"
                     /old_locus_tag="P3U71_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(14669..15345)
                     /locus_tag="P3U71_RS13625"
                     /old_locus_tag="P3U71_13625"
                     /pseudo=""
     CDS             complement(14669..15345)
                     /locus_tag="P3U71_RS13625"
                     /old_locus_tag="P3U71_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            15756..16499
                     /locus_tag="P3U71_RS13630"
                     /old_locus_tag="P3U71_13630"
     CDS             15756..16499
                     /locus_tag="P3U71_RS13630"
                     /old_locus_tag="P3U71_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843758.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNAR
                     QNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSANPND
                     NDSDIEEKSEETEPKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(16718..16903)
                     /locus_tag="P3U71_RS13635"
                     /old_locus_tag="P3U71_13635"
     CDS             complement(16718..16903)
                     /locus_tag="P3U71_RS13635"
                     /old_locus_tag="P3U71_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000466619.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            17052..17123
                     /locus_tag="P3U71_RS13640"
                     /old_locus_tag="P3U71_13640"
     CDS             17052..17123
                     /locus_tag="P3U71_RS13640"
                     /old_locus_tag="P3U71_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106873653.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(17362..17703)
                     /locus_tag="P3U71_RS13645"
                     /old_locus_tag="P3U71_13645"
     CDS             complement(17362..17703)
                     /locus_tag="P3U71_RS13645"
                     /old_locus_tag="P3U71_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000761341.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(17790..18650)
                     /locus_tag="P3U71_RS13650"
                     /old_locus_tag="P3U71_13650"
     CDS             complement(17790..18650)
                     /locus_tag="P3U71_RS13650"
                     /old_locus_tag="P3U71_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_054105168.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNIFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQL
                     ELNWE"
                     /name="RepB family plasmid replication initiator protein"
     gene            18708..18992
                     /locus_tag="P3U71_RS13655"
                     /old_locus_tag="P3U71_13655"
     CDS             18708..18992
                     /locus_tag="P3U71_RS13655"
                     /old_locus_tag="P3U71_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001793642.1"
                     /translation="MKTFIHNKLLYYQCQTIKGVQLFKTLLLCGKNTPFMWQKHSFYVA
                     KTLLLCGKNTPLGIEIPCCSRAEQAPKKVFKKIYKNILKSSRGATYFSS"
                     /name="hypothetical protein"
     gene            complement(19034..19579)
                     /locus_tag="P3U71_RS13660"
                     /old_locus_tag="P3U71_13660"
     CDS             complement(19034..19579)
                     /locus_tag="P3U71_RS13660"
                     /old_locus_tag="P3U71_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000273005.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMILD
                     DYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKE
                     LNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQ
                     AQEREIIFKEDREDGYTL"
                     /name="hypothetical protein"
     gene            complement(19673..20302)
                     /gene="mobV"
                     /locus_tag="P3U71_RS13665"
                     /old_locus_tag="P3U71_13665"
                     /pseudo=""
     CDS             complement(19673..20302)
                     /gene="mobV"
                     /locus_tag="P3U71_RS13665"
                     /old_locus_tag="P3U71_13665"
                     /inference="COORDINATES: protein motif:HMM:NF013258.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     ARG             complement(4457..5301)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          complement(14172..14319)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.333"
                     /coverage="100.0"
                     /evalue="2.96e-25"
     Typing          15757..16499
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(17791..18650)
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.884"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(20028..20302)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 ttaatcttgc caatgctact ctttgttgtt caccaccgct tagagaacta acaattctct
       61 tgatatctaa atctaggccg acctttttta atacttcttt ctttaagttc atattatctt
      121 ttttattctt tttgaacttt agggctaaat ctaaattttg actgattgtt tgattttcaa
      181 gtaaagcaaa attttgaaac aaatacccta acgtatatct ataaaaatcg actttctttt
      241 ttagtaactt atcttcaata tatatgcttc cttgtgatac atcttcgaac ccagcgatta
      301 tgttaagtaa agtagatttt ccagcgccac tttctccaac taaagcatat gattttcctt
      361 tttcaaaatt aaagttacat ccgtctaaaa tgtttttatt accttttttt acaaaaacat
      421 tttcaagttt tatcactatc ggtcacctca ttttaaaaag ctaggaatat cttttgtaat
      481 gtattctatc gaacgcacat atttcccttt atgatttact ttcacatatt cttttgtagg
      541 atcataacct aaaaaatcca atttataact ctttttttct ccatttttag agtatacagt
      601 tatatttcta tagtcctggg tatcttttgg aactttggca taagatactt tttcttttaa
      661 cagtgggtta aaacgatcta aatgctcatt tctaaccaat aacagactaa gtatgatgat
      721 taatagtaca attgttacaa taattaaagt tcttttcatt gtttaaatct ccctcattaa
      781 atctttaaag tatttttcaa gatatttaat ataagcaatt tgaagtaaat gttgaataat
      841 agcaatagac attattatga atagtaatat ctttgaacca aataaaatat gcgtagtgta
      901 agctaacata atagaaagta agatagaaat tgttaaataa cgtacattac tgctgaataa
      961 tgaataacca tgaatttttc ttaataatag ttgcttttta ttccagctaa aatattgaat
     1021 tgtttcaaat acagtagcta tcaaaagaat gaaaacgtta ataataatag taactattgt
     1081 tattatcatt tttgttttcg tttcatttaa ttcatttaac acagaatcct tgtaattagt
     1141 tataccgcta atttcttctt ctaggtgatt atctttaagc aactgtttaa gtgtatctaa
     1201 atctttaaat agatatccac cttgagaaac agcagctaaa taaaaatctc ctgttaaatc
     1261 gtcaagtgtc aacaacatta ctattggaga atctagatac ccaaatttca aattagttgt
     1321 gcgatcaaaa gaaaaaacag atacgttttt tgacagtact tgcacatcta cttttttgtt
     1381 ttgttgcttt tgaaagtcaa tccatccttg gtgatttttt tgaatttcag atttttgata
     1441 ttgtaacctt ggaggaataa acacgtttat tttatccgaa tgattgtcaa tcattttaaa
     1501 atcatgattt atatttttat agaaattcag aaaattgtta ttgataaaaa agacattgcc
     1561 attttcagga atgtaattgt ttttatcggg attggctata ttattattct ttaaaagcaa
     1621 tccaccaagt tgttcgcttt tcttaattaa attgtatata ttagtttcta aaatttgttt
     1681 ttcttctgaa tttctaatat taggagaaat ctctaatgta tagtaattat ctaacttttg
     1741 ccagattttt tcagagtcat atatattctt taacttcgta taagcattta aattttgtgc
     1801 cagtaaaaaa gatagtatta gcaccatgat acatttggtg aaggtaccca taaatattga
     1861 aaacttatat ccttttttcc cttttaagat taatggaata cttaacttca aaagaagact
     1921 atacgaaatc agccacagca atgtagtaat acaagcaaaa acaaaaagca acacgataaa
     1981 tctacgaata aacattaaga aatccaattc taaattggct atatgaatga ttaatatatt
     2041 agcaataaag aaaatagcgg taagtactag ccaataaatg atttttatat gaatattatg
     2101 aaaaataatg cgtgtagttg aaaagccatg tagcttttga atagcataaa atttcaaatt
     2161 atatcctctg tcatataaga agtacaaaag atataatacg cctaagaata tgaaaggcac
     2221 taataaatca ttatctatga ctaattcaaa aaataatatg tttctattta tgtattcaac
     2281 ttcagatgtt aaaccagctt tttttaattc actcgtaagc tcttctacat tgaaatgttc
     2341 accaataata taatactttc cttttatctc tctgtttaat aaatctgatg gagttaaaag
     2401 tgatgtttta ttacgtataa agtcagaatt atttcgtttt gagttgaaaa caaaaaattc
     2461 ttttttatta tcaaaatctt taacggattt aattatttgt atattttgat ttttggcgcc
     2521 tgattcaatg acatctaaca cttttgattt actgttagat ttatcccaat tagagattgt
     2581 taaaatattg gaactttcag gtataatttc ttcattttct ttgaagaaaa ataaaaataa
     2641 tagccctgaa attaataata aagtactaaa atctaaaaat aatttgaatt ttttcatatt
     2701 gcctctttca agagaataga cagaattaag tttacttaat tctgtctatt ctccttaatt
     2761 aatatatgta attagtgaag actataaaat gttttgtttc caccccaagc ctttggagca
     2821 gatgccttag attgaacacc aggtcgagca acaccactaa aagatgaata tttccctact
     2881 gctgttgaac catgatttct tttattgtga ctataataag accatacgta atgttttcca
     2941 ataccatggc tccaaacacc accagctaca tgtactgttg cagcttcagc tttataagtt
     3001 acacctaata aagtgccgaa taagattgta ctagcaatac aactagaaac aaatttcttt
     3061 tttataaaaa cacctccatt taatattctt tatttattat agcaaaaata aataaaatag
     3121 agaatgagta aaaataatct aaaagtgttt tttctgctct gttgcttgtt ttgttcatct
     3181 atatataata ttaattctat tatccttaaa ttaactatca ttaaacaacg atatgacaat
     3241 gtataataaa tacaatttca ctacctataa ttcaacatta tataaactaa agtatttcaa
     3301 ataattaact gtattaatac tctcatcatg attgcgttat aaacattcgt taatgagaaa
     3361 aagagagagc atatttacgc tctcttttct tgtcttatta ttaaaaactc ataatagatt
     3421 ataatcatct gttttgagac gctgtgagaa gctctcagtt gcattgaaac aaagtttatt
     3481 aaatcaaatc attatcgttt ttaattctgt aaatcgtctg ccttgttata tttacttctt
     3541 tcgcaatttt actaattgat tggccttctt ctaacatttc tacaacgcga tgataaataa
     3601 tacgtttctg tggatcttta gcatttggtg aataaagtaa ggggcgccct ttatatacgc
     3661 ctttttcctt cgcaacttga attccttgcg cttgtcgacg tttactttca tttctctctt
     3721 gttctgaaac cattgctaat atttgaataa ttaaatcttt cataaactta tccagtaatg
     3781 gattgccaac cacttcattc atcataggta agctagtaat cattaattgt acttctttgt
     3841 cttttaaata attaacggta tgaatcactt catcataatt acgacctaaa caatcaatgg
     3901 attctactac aaaccgatct cccatcctca aaaagttaag tgcttcttga agtactggtc
     3961 tattttcaat agatttccca gactgttttt ctgtaaatat tttttcagca caaaacgtct
     4021 ttaaattttc aagttgtctt tctaaatttt gatcgttcga tgatactctc gcatagccaa
     4081 caatcattga tttccatctc ataattaata tacaccctaa tcatacgttt cagttactat
     4141 aaattcaaca attatgtcac tttgcttgat atatgagaat tatttaacct tataaatgag
     4201 aaaaaagcaa cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat
     4261 taaactattc atctattatt tatgattttt tgtatataca atatttctag tttgttaaag
     4321 agaattaaga aaataaatct cgaaaataat aaagggaaaa tcaggttttg atatcaaaat
     4381 tatacatgtc aacgataata gaaaatataa tacaaactat aagatgttat cagtatttat
     4441 tatgcattta gaatattaaa attccttcat tacactcttg gcggtttcac ttatcaactt
     4501 atcatttggc ttatcacttt tattgtcttt attcgtaaaa atgactaaaa caataggttc
     4561 agattggccc ttaggataaa caaaagcaac atcatttcta gaagcatatg ttattgcttg
     4621 accactttta tcagcaacct tatagtcttt tggaacacca tctttaatta aagtgtctcc
     4681 gtttttatta ttaaacatta aatcaagtaa gaaattttta ttttttttgc ttaattttcc
     4741 atttgcgata agtttattta aagtcttgcc gaaagcagca ggcgttgaag tatctttttt
     4801 gctctttggc gaatagtaat ttaattctat ctcatatcta actggatttg ttactttatc
     4861 tcctagttct tttagacgtt gtttaacttt tttgattcca ccgatttctt ttataatttt
     4921 attgtttgct gtattatcac tatatgtcat tgaagcctca ataagttctt ttaaagtgat
     4981 gtcttttcct acatattttt ctaaaatagg agaataagca actatatcat ctttgttaat
     5041 atgtactttt ttatttaact tattataagg tacttgttct aacaaaatag cactatttat
     5101 cgcttttgaa gtcgaagcat aggcaaatct cttatctgaa ttaaatttta cttccttacc
     5161 acttttagta tctaaagcat aaacaccaat atgagcatta tatttttttt ctaaatcatt
     5221 taactctttg gcatgtgaac tgtttgaatt acatgcactt aaaactaaag ccattacaat
     5281 taaaaatatt aactttttca aaataaaccc tccgatatta cagttgtaat ttttacaaaa
     5341 caataatatt acaattgtaa tattgatgtc aattgtttat tataaatatt tgaaagaagg
     5401 tgtcaaaatg gctaaattac taataatgag tgtagtaagc ttttgtttta tatttctatt
     5461 gttagtattt tttaggtaca tattaaaacg ctattttaat tatagtttaa attataaagt
     5521 ttggtatcta actgtgcttg ctggattaat tccttttatc ccaattaaat tctcttttat
     5581 aaaatttaat aatgtgaata atcaagcgcc cacagttgaa agtaagtcac acgacttgaa
     5641 ccataacata attaccacca aacctgttca agagttcgca acagatatcc ataagtttaa
     5701 ttgggattca attgataata tctgcacagt tatttggata gttttagtta ttattttaag
     5761 ttttaaattt ttgaaagcct tattatatct taaatattta aagaaacagt cactttatct
     5821 aaacgaaaat gaaaaaaata aaatagatac gatacttttc aaccatcaat ataaaaaaaa
     5881 tattgtgatt cgaaaagcag aggctattca atctccaata actttttggt atgggaaata
     5941 tattattttg attcctagtt catattttaa aagtgtaatt gataaaagac taaaatatat
     6001 aatactgcat gaatatgctc atgctaaaaa tagagatact ttacatttaa ttatctttaa
     6061 tatcttctct attattatga gttacaatcc attagtacat attgtaaaaa gaaagataat
     6121 tcatgacaat gaagtagaag ccgatagatt tgtccttaat aatattaaca aaaatgaatt
     6181 taaaacttat gcggaatcta ttatggactc cgtattaaaa acctcatttt tcaacaaaaa
     6241 tatattaagc cattcattta atggtaaaaa atcattactc aaaaatagat taattaatat
     6301 aaaagaagcc gatctgaaaa agcagtcaaa attaattcta atttttattt gtatttttac
     6361 tttttttatc atgataattc aaagccagtt tttaatgggg caatccttaa ctgattataa
     6421 ttataaaaag cccttacaaa gcgattacca aattttagat gaaagtaaaa attttggttc
     6481 aaatagtgga tctttcgtta tgtacagcat gaagaaagac aaatactata tttataatga
     6541 aaaagaaagt agaaaaagat attctcctga ttcaacttac aaaatttatt tagcactgtt
     6601 tggacttgac cgtcatatta taagtgataa aaattcacgt atgtcatgga atcataaaca
     6661 ttatcttttc gagtcttgga ataaggaaca agatttaaat acagcaatgc aaaattcagt
     6721 taattggtac ttcgaacgta ttagtaatca aataccaaag aactatactg cggctcaact
     6781 caagcaatta aattatggta atgaaaattt gggaagttat aaaagctatt ggatggaaga
     6841 tagtttgaaa atatctaatc ttgaacaagt aatagttttt aaaaatatga tggaacaaaa
     6901 taaccatttt agtaaaaaag caaagaatca attatcttct tcattattga ttaagaaaaa
     6961 tgaaaagtat gaactgtatg ggaaaacagg tacaggtata gtaaacggga agtataataa
     7021 tgggtggttt gtaggttacg taattacaaa tcatgataag tattattttg ctacacattt
     7081 atcagatgga aacccgtctg ggaaaaatgc tgaattaata agtgaaaaaa tattaaaagg
     7141 aatgggtgtt ttaaatggcc aataagcaag ttgaaatatc tatggctgaa tgggatgtta
     7201 tgaatataat atgggataaa aaatcagtat cagctaatga aattgtagtt gaaattcaaa
     7261 aatataaaga agttagtgat aaaacgatta gaacattaat cacaagacta tataaaaaag
     7321 agattataaa acgatacaaa tcagaaaata tttattttta ctcatcaaat attaaagaag
     7381 acgatattaa aatgaaaact gctaaaacct ttcttaataa actgtatgga ggggatatga
     7441 aaagtttagt gttgaatttt gcgaaaaatg aagaattaaa taacaaagaa attgaagaat
     7501 tgcgagatat tttaaatgat attagtaaaa agtaattaga ggtatgtttc aaacataata
     7561 catatgtaaa ctatgcttga ctcttattaa attagttttg ttaatgacat ttaaaatata
     7621 ttattaaaga tgagagtgta cagaaaataa ccattttctg tacactcttc ttttatgtct
     7681 aaataagtaa gaattacata ggattatgtg ttcaaaaacc caacaaaaaa tctatgttca
     7741 taaaagacct tgtaattaag ttatggtaca ctgaagatgt atataggagg tttgtatttt
     7801 gaaaataggt tatgcaagag tttcaactgg attacaaaat ttaaatttac aggaggatcg
     7861 attaaataca tatggttgtg aaaagatatt taatgaccat atgagtggtt caaaaagtaa
     7921 aagacctggt ttagataaag caattgaatt tgcgaggtca ggagatacga ttgttgtttg
     7981 gagattagat agattgggac gtaatatgga ggatttaatc acattagtta atgagcttaa
     8041 cgaacgaggt gtaagtttcc atagtttaga agaaaatata acaatggata aatcaagttc
     8101 aactggacaa ttattatttc atttattcgc agcatttgca gaatttgaac gtaatttaat
     8161 tttagagcgt tcttcagctg gccgaattgc agcacgtgct agaggccgat atggaggcag
     8221 accagaaaaa ttaaatcaaa aagatttaaa cttacttaag accctttatg ataatggcac
     8281 gccaattaag acaattgcag aacagtggca tgtttcacgt acaacaattt atcgttacct
     8341 caataaattg gaggacaagg aagatgaaaa acaaggagaa gtatctaact aatttttctg
     8401 aagccaaacg taaaaaagct actcaaaagt acaatattat aaaacctttt atcctaggcg
     8461 aacaatcttt atcaagtatt tctaaaagta aagggattgc gttaagcacc ctttataggt
     8521 ggaataagtc atataaagaa caaggactta aaggactaat ctatgcgaca agagctgata
     8581 aaggaacacg taaaatagaa ccaaagataa ttgatgaaat tgaacgcctt gcgcttatga
     8641 ataaaagaaa tagtatagcc acaattcata ggaaaatcac taattactgt aaagaaaata
     8701 attttgatat accaagttat aaacaaattt atagtgttat taaagcgatg cctaagtctg
     8761 ttattgattt ctctcatcag ggtgaaaaat actatcaaaa taagtacgat ttaatacaaa
     8821 ttagagaatc ctcgagaccc aatgaaatat ggcaagcaga ccatactttg ctagatatct
     8881 ttatattaga ccaaaaaggt aatatcaata gaccatggct aactattatt atggatgact
     8941 atagtcgtgc aattgcaggc tatttcttaa gttttgatgc cccccaatgc tcaaaacaca
     9001 gcattaacac tacaccaagc aatatggaat aaaaatgata ccaattggcc agtgtgtggt
     9061 atacccgaaa aattctacac agatcatgga agtgacttta cttcacatca tatggaacaa
     9121 gtcgctattg atttgaaaat taacttgatg ttttcgaaag ttggtgttcc tcgtggtcgt
     9181 ggaaaaatag aacgattctt tcagacagtt aatcaaactt tcttagaaca actgcctgga
     9241 tatataaaca ataatgatac tcctagtaat ctaataaatt ttcacaattt tgaagaaaaa
     9301 ttacgttact ttttaattga agattataat caaaaagaac atagtactat tcataccacg
     9361 cctatcagtc attggaatag taatcacttt ttccctaata tgccaagtag cttagaacaa
     9421 cttgatttat tattgttgga aatacctaaa tctagaaaag ttcattcaga tggcattcat
     9481 tttcaaggtt ttcgatacag taatactaat ctagaagctt atgtaggtga atatgtactg
     9541 attcgttata acccaaatga tatggctgaa atacgtgtgt tttatagaga tgaatttctt
     9601 tgtactgcta tatgtccaga aatagctgat tactccatag atataaaaga catacagcac
     9661 gctcgtagtc agagaagaaa acacttaaaa caaaatattg ctagtccaag taccacggaa
     9721 ttaattaagg aagaaaaaaa ttgtggttat tcacctcaag aaacgactaa aaacgtcaaa
     9781 aaattaaaga gatatcgtaa tgactaaaaa tcaaaatttc attgaaacga aagagtataa
     9841 acgctttgct gaattttgtg atgcttgtat taaatatcag tatattggta tatgttatgg
     9901 tcaacctggt gtcggaaaaa ctttatcgtc taggtattac acaaattggg atactattga
     9961 aaaacaagtt aaccatagag gttgggaaga tttagctagt aaaacgaccg atgacatact
    10021 atcggtggat aaaatatttt ataatgcacc agcagaaaaa caaactaaat taaggaatga
    10081 tttatatagt attactgcaa tcattgattt gggtcaaaaa ttacatgttg taaataagta
    10141 tggacatgaa catagtaaac attatagtga tatgtttaaa tatattgatt taattatagt
    10201 agatgagatt gatcgcctta aagtgcaaca tttagagcaa ttaagagcta tttatgatga
    10261 acataattta gcaatgatat ttattggtat gccgggtata gagaaaaaac tatctcgtta
    10321 tcctcaacta tattcgcgta taggttttgc gcatgaattt gataatctaa gtaaagatga
    10381 gacgcatcat atattagaat ataagtggca agacttagga tttgatttaa aactcgaaga
    10441 ctttactgat tatgaggcaa taacgacgat tattaaaatt acaaaaggga attttaggct
    10501 gattcatcgt ttatttgcgc agatagacag aattatggat ataaatggtt tagataaaat
    10561 aagtacagaa gttgtagaaa cagctagaga tagtttagtt ataggtattc gataatagaa
    10621 aaagcaacgc attttaatat agtgcgttgc ttttctcatt tattaggtaa agtcattctc
    10681 atatatcgag taaagtgaca acaattatat atgtatgatt agggtgtact tatatcattc
    10741 atataaatgt ttatttatac cctcaataaa aatattatat gcttttaagc acctatcaca
    10801 ttccttataa aaatgtttat tcaatccttt aatatcgact agcgtagtct tataattttt
    10861 ttgtaacaat tttaaatcct cttcgtaaaa gcttaattta aaatgaccgg cttgagctaa
    10921 tggctttttt ccttgtttaa ttaaaatcaa tatgtttttt aaatgtttta aaaagttttt
    10981 ataatcataa acttttattt tcatgaatga tttatgtgga ttattttcat aatttagcag
    11041 gtagttttct atatcttcaa tataactatc atcaaatggt gtgtgctgga aataaaaagt
    11101 ttttacatac atgaatattt tataaagttc ttctttcaaa aaatcatatg tatctaaatc
    11161 tctttgtata gcatccaatt tttttaattt ttttacattt ttaccacaat cctgtactct
    11221 tatccatcca ctattttctt taatttcact aggtacactt tgcattaaag tctgagatac
    11281 tttacttttt aattccgaaa tatcattaat atttggacta attagtctat tttctttaac
    11341 ttttgtctta aactcatcta attttttaga cttttccgcg gattcggtag atgctttatt
    11401 catctcaaaa acatatatag gtttacctaa agatttagca aaattgtact ccatttctgt
    11461 atagctaata ttttcttcac tatcaattgt tccataacta gaacctaaca ataatatcac
    11521 tgcatctgat tcattgatcc attgtgttat tatttcccat tggcttttac tacttgagtt
    11581 aaaaagttcc atacctgctg gaatattatt actgtttaaa actgcttcta ctactgcttg
    11641 acgctcttct attaaatcta aataagttga tgaaataaaa atttgatatt taattttagt
    11701 attactcata tatgctcctt taataaaaaa tcaatttaca taaatatatc atatgataat
    11761 atccaaaaag actacttaaa atattatctt tttgcattcc tctactaacc agtgttataa
    11821 tctagataac gaaaatgatt aacaattaat gcaccaatcc ccacactact gcaatagtgt
    11881 ggggattttt tcgatgctgg cttataaagt cgcctattaa ttatttgttg cgtttactaa
    11941 gccaataagt ataatacgca acagcacagc cactgatgac tggtgctatg atgtgaacga
    12001 aaatgattag caaagtaatg cacctcctct ctgcgtctga attgacgcct gagagatagg
    12061 cgacctgtat attatatcat ctccgaatat acgatgatag ataacttccg ataagtcgtc
    12121 ttatgttata taactttgtt ttttatagta tcaagacaag aagaaactcg tttcactcgt
    12181 ttcaaaaacc ctcctgattt ttagagaaaa actctcttat ttcatcgtct ttaataaaat
    12241 aatatgccat ctttccatct ttataaaaat caagtacctc atttttatat aaaagcctta
    12301 aatgatgtga agttgaagca acactcattt tcaatatcaa agaaatatca caaacacaca
    12361 actcatcttc tttaatcaaa gataatatga ttttcaattt cttctcatca caaatttttt
    12421 ctaaaatgtt aagtaatttc ttagatttat catcttctaa aaaacttaaa gcattgttaa
    12481 ctttatcctc atgtacacag atcacatcac aagtattttc ataactcata taaaatcaca
    12541 ccctaaatta aaatataaat cctaaaattg tttgaatagt gtcattttca ataataataa
    12601 ataaacctaa agctatataa atgatagcca taatccaacg actaaatttc tcaacaattt
    12661 ctccaattcc tgggatatta gctaattttt gtgcagtaaa caccaagaag aaaattaaaa
    12721 ttaaaaagac aaacagagta agtaataaat tagtaacact taatgtcaca aaatacggaa
    12781 caaataaacc aatattatcg gcgccacaac ttgctatcgt aacaattgca accgtaccaa
    12841 ctaatttaga caatcctttt tcattcaatt cttttttagc tctcttttct ccttcacaat
    12901 catcataaat agccacttta attccaagat aaattggtat taaacccaat aaacctaata
    12961 tccacttttc tggaacataa tttaatacaa aagctaaaaa caaactaact aatattaaaa
    13021 taatagaccc taaatattga ccaacataaa tatttctata ttcttttcta gtctttgctc
    13081 tagcaaaaaa tattaataaa attactaata aatcaactgc tgtagcaata taaagaacag
    13141 cagcagcaac aaccgtttga atcataatac acctcattca aaaatatttt tgaatgtatt
    13201 ataacttata tttttaaata atcataggtg ctttattcca attgctttat tgacgttgag
    13261 cctcggaacc cttaacaatc ccaaaacttg tcgaatggtc ggcttaatag ctcacgctat
    13321 gccgacattc gtctgcaagt ttagttaagg gttcttctca acgcacaata aattttctcg
    13381 gcataaatgc gtgtctatgc gtagacatgg agacacttca gaattatgga gtgattacat
    13441 gagtttaact atttttcttt attcagtttt aggaagttta ttagcaaatt atatctacga
    13501 aaaatcttca aaataaataa ctttttcggt tctactttgt acactttatc tacctggtag
    13561 acctaattgt tattgcttta caggtctaaa attcgtcgtt ataatctagt taaatcccgt
    13621 aagggcgcac ttatacgtcg gataaatcga catgtgcaaa aacgtaatcg accttcgtcg
    13681 atttttttat ttcaaaatta aagaaacggc ttgagatatt ccgagcagtt ataaaaatta
    13741 atttgcgttg aatattgcta ctacattctt gaaaaagtga tgtagattta tagcccataa
    13801 cttttttgaa ttacatcgac gatacggcgt cgaaagaaaa agctacctca ctttatctgg
    13861 gggggctttt attttttatg cccttatttt gaattttaag agggcatttt aaagatttag
    13921 gggtgattca tatagtttta tatctaaaac ttatatacgc ttttaaaacg caaatatgag
    13981 ccaaataaat atatgctatt ttcaaaaaca aaaatttgag caaaaacaat aactattttt
    14041 tagacactgc ccatttacat gcaaattaaa aattagcata aaattcttta aaacttcccc
    14101 acgttcttct aaattcctgc agggcttttc ttatcaaaaa cttagaaatt ttaatttaac
    14161 tctaaaatct ccgtaagagc ataagggaaa tttgtcccca tcaatgaatg ggggatctgg
    14221 caaagccaga tatttcaagg cttcgaaaat gtttttgtgt ggggtcataa aaagctcaag
    14281 tgtcacactt ttgtgacact acatacagca tttgtcacaa aaataagaca gcactttttt
    14341 tacaaaaaag taataaaaaa agacatcagt tatcactaat gccttaaata ataattttgc
    14401 ttttcaaaat tgccgtaatc gaatgtgcct tttcttcttc atttgctatt ttatcttcat
    14461 cacttgtatt aattaaattg ccatcttcag catcgtctaa gtttaaaatt ttatgttttt
    14521 atttaagcaa tccaccataa cttaacatac gttttcgata caaatctttt tctagatcac
    14581 tgacaatttc ttgatttttt catcgctatc agttaaaaag tcagatgatt taacgaaata
    14641 ttttgatgta tctttgattg ttataaaatc atttgaatga ttgattaaag atattatatt
    14701 gtttcatgtt tttacggcgc atataaagtg catgtgtttc ttcaaattca gttaaaattg
    14761 tttgcattaa ataaccttgc attgacttga atgattacta atttcatttt cggactctat
    14821 atcattcaag tcattcatat catttatttc agtattgtta gattcagtat agctaagttc
    14881 agtatcatta gattctgttt tatcgacttc ttgaagtcgg tttttccgac ttccttgcca
    14941 ttgctgctct caacagtgtt ttcttgcttt tgaattttat aaatatcatc ttctgttact
    15001 tctggtttaa ctaaataaag tttatttgtc ttatttaaac cttgtctttc ttgtagtagt
    15061 aattcggctt caattaagtt ctttctttat tttaattact ttatcttttt tacaatttaa
    15121 tatattttgt gggttttcat ttttgtaata aaaataaata ttacctgttt tagaatcaac
    15181 ccaaccattt ttaattgata aatctaaacg atctctaaga ataccccatg ccatttttgc
    15241 atcattggat aacttgatat atttaggatt tgtaaaaaat actttcggta tttgataaaa
    15301 cttttctctg tattcctcat ttatgttgta aagtttaatt gacataataa aaacctcttt
    15361 ccgaatttta gtttggtaag aggtaataaa acgcttacat tttttgtgtt tatttaataa
    15421 tctaggtgct tgcactaaga tattattcat tatataatct aagtatcgaa aattatataa
    15481 ataaacagtt tctaaacgtt ttattaccaa accccgacta ttaccagtag ttggggtttt
    15541 tgttatatta aggttattat agcactttga ataaacttaa aaaagggaaa atataggttt
    15601 ataaaccttt gataaacacc ataaaccctt tacaaaccct tgataaaaag gttggcgaat
    15661 ttatattttt atttgtcgat tactttatta ttaatcgtta taagcttata atattttact
    15721 aagggtttat aaacatttta taaagggttg atgatatgaa aagtgtaaaa aggttgagtg
    15781 aagaattagg ggttagtaag caaacgattt ttaataatat taagagattg aatatagaga
    15841 caataaaaca ggaaaacact tcgtttatta aagaagatac cgatatagaa aaaatcattc
    15901 aaagagtaaa tgaaaataaa aagaaatatg gttttgaaag tgctactgaa gacaaacaaa
    15961 aaaaagagtc ggataatatt aattttgaat ctaaaagcga tgctcaaata gtagaaatac
    16021 ttaaaaatca gattaatact ttaaataatc aaattgaaaa acaagaaagt cgtcatgaaa
    16081 caacaattga attttacaga aaagagttgc aagaaagatc aaaattgctt gagaatcagc
    16141 aagttctggc attagagagt aataaaaaga ttcaaaaatt agaaaatcaa ttagaaaaag
    16201 aaaaacacct taattatccc tttgatacat ctactaacgc tagacaaaac gttgatgcac
    16261 aagaaaaaac atatactact agtcctgtaa atattaaccg agatcaggaa gaaactaaag
    16321 aaacagagat tcaatataag gatatatctg gaagtcaaag cgacgaaagt acacaaggtg
    16381 aagaagcaca aagagaagac gtttcagcta atccaaatga caatgatagt gatatagaag
    16441 aaaagagtga agaaacggaa cctaaaaagg ggttttggag tcgtctattt ggtaactaat
    16501 tttaattaaa aaggtacgac aagttcataa attagccgtg cttttttatg tgaattgaac
    16561 ttctgataat catcgattat gttaaccaaa caaaaagcca taaatgccta tatgacggca
    16621 tttatggctt tttactttca ttatctttta tgcatctttt atacatcgtt gatttgacgc
    16681 gttttcattc ttttgcattg gcaagtattt tgtatcttta ttcagttact atttcttttc
    16741 tccaacctaa caaaccatct ttaactctaa taatatgttt gccacgccag acacttctta
    16801 cttcgaaata ataccattcc ccgtttttgt tgaggaaata aatatcttga taaccttctt
    16861 taatgacata ttttgtagct tcttgtcttt gatcgccaaa cattatcaat cccaccctta
    16921 tatttattta tctttaatat ataacaacat ttattaatat tcatgttatt tcatggaagg
    16981 agagatttgt tataatgtga aggtcgtcta tctctcagat gtcaattcgg cgtagagagg
    17041 aggtgcgatt tatgaatgaa attcttgttc acatcatgac aacggcaatc agtggttgtc
    17101 tcgttacgtt atttggttat tagctccata aacgtgataa aaaataatat agacgattaa
    17161 agccaattca ccaaaaaacc ccgaactacg gcgaatagtt cggggttttg tgcgtttaca
    17221 aacgaaattc ttgttacttt tagtatatat ttcatattgc cttttgtaaa ctcttctatt
    17281 cttatcaata catttcaagg tgtcgtaaaa atactttcct ttttcatatt gcctgatgtg
    17341 cattaaattt tgattttaaa atcaacccat gaacgaatga atggcccaaa ctaaaaatcc
    17401 ggcaacagga aagaaaagcc accaaaaatc ataaagaaaa tcaactatgg aaggtaccct
    17461 gttactctct ctataatttc cctctaaaat aactttttta aaacgtgcat ctcctttaat
    17521 taaatgctct aaagagattc cgaataaatc gctcatagca atcaaaatat ctaaggaagg
    17581 atagtttttg gcactttccc atttagaaac actttgtctg gaaaccttta atatattggc
    17641 taattcctct tgagaccatc cttttaattc tctctgttct tttatagctt cagctaattt
    17701 cataaaatca ctccttaact caaactataa ttaaaataat tgaaaagtca cgatactttt
    17761 agttaaccaa ctcgcaacga aaagttgcgt tattcccaat tcagttcaag ttgttttaaa
    17821 aaatcctctc gttctttctc taattgtggg tcgtaatctt tttgaggttg cttttcatat
    17881 gaccgttctt ctaaccattt aggcgtcttt tcacgcctca catattgtct tgatttatct
    17941 atcttggaca tttgaggttg tcgtttgtta tgaatgcgtt tctcagcgtc aaatgtgaac
    18001 tctatccatt caattttacg cccttttttc gctttgattt tattgatagt aagattatta
    18061 aatatagaac ctaattcaat aataataggt ttaaaaatat ttttgttaat atcagtcata
    18121 cgataagatt taggtatatc taaacgattt ttaaaatcat cgatgtgaat ttttaaatag
    18181 cctgtatgtt tgtattgttt aagtaatcta aacatatttt tagaatatgt agatttaagc
    18241 ctagtcattt cttttaattc aaattttgta aaattattag taattgaatt caatatgtgt
    18301 tttaaatttg aagaagttga tatttctaga tattgttctc gcttatggat tctataatga
    18361 ttgaataaaa cgaatctttc tattacatca tcatcttcat atctaatggt taaattcaac
    18421 atctttttat acacattatc taaatcatta gcaaatctat taatattacg tgtttctgga
    18481 ctataattac ttaatttttt taattcatca aaagacaaac gtaatgtatt agtatcttgc
    18541 tcttttaatt tattacacat cgcaaaaaat aaattaattt cagtagctgt aaatcgcctt
    18601 aatggaacta aattcatttc atttttatat acaactgtct ctcctgtcat gatacagccc
    18661 cctttattac gaacctaaag gagtatcgtt tgaatataca ctcccatttg aaaactttta
    18721 ttcacaataa actactttat tatcaatgtc aaaccataaa aggagtacaa ctttttaaaa
    18781 cactcctttt atgtggcaaa aacactcctt ttatgtggca aaaacactcc ttttatgtgg
    18841 caaaaacact ccttttatgt ggcaaaaaca ctcctttagg cattgaaatc ccttgttgct
    18901 ctagggctga acaagcccct aaaaaggttt ttaaaaagat ttataaaaat atattaaaaa
    18961 gttcgagggg ggctacctat ttttcatcct aaaaaataat taacctcaaa caaatgcatt
    19021 taaatatcta tttttataat gtataaccat cttctctatc ttctttaaaa atgatttctc
    19081 gttcttgtgc ttgatatttc ttcttaaaga actgttcatc attgttatct atcgtcatta
    19141 cttcacgtat tttagaattg tctaaatgat tatcaatatg atttattagt gtatgataac
    19201 gctcttcttt catcatactt ttaatgattt taaaaacttg attgaataac ttaagcgtat
    19261 aatttagttc tttatctttc ttttcattct ctgacaacaa ataattataa cgttgtgcta
    19321 aatcattaaa ttcttcaacc agcttttcat tttccatatg gacactagtc aattcattaa
    19381 ctaaactttc attttgtttc tctgcttttt ttaatgactt cccactttta atacattcat
    19441 aatcatctaa tatcatctgt gctgctttaa tttgtttagt gtatttttca aaatcagttt
    19501 tagtaattac aatattgcct gtttcttgct tctctttatg aaaccatcca cctacatttt
    19561 tagttttaat ttcatacatg acactcagaa gctttttaag cgtatctaat gatttttggt
    19621 agttttgtat caatttatca ttttcttgct ttaaataatc ctctatttgc ttctagcgca
    19681 tatgcatttg tttatgatat tctgtttttt gtttataacg gctaacttga tcatgttttt
    19741 cttttgtaag ttgtctagaa attcctcgtt ttaaatcata gccttgctta ttaatatacg
    19801 tattgaatct atcttggaat tcggttaatg cttttttatt acctacaact tctttcgcac
    19861 ttaaacgtcc atctttagta ataggaacta cgccataatg catatgtggt gttatctcat
    19921 ccatatgtac tgtcgcatat aaaagatttt ctttaccgta ttcttcttcc aagaacgctt
    19981 tagcatgctt aaaaaactat tttgtctctt cttcagataa ttgattaaaa aacacattat
    20041 ctgatgtaat taatccatca atatgtttaa tggcatctgt tctaattttg cgtttccctt
    20101 tataattttt ttcgattttc tcatcaattt ttttattaaa atcaaactta gtatcattga
    20161 ctaaatcata gtttaaatat gacttactta aatcaatatc tatattttca taatttttat
    20221 tttctctttg aatatgtctt tgaatacctc ttgtatttgt ttttgatttc actttaacaa
    20281 ctcgaataat tgaataagac ataataaatc aacttccact gcatatgatt ataacaccta
    20341 aacacagaaa agttttaaca tactagactt attaaaaacc aaataagtca ttaaaaacgt
    20401 gtgctctgcg aggcttgttt tttgtcatca taaaaaagga ctggaaaatc cagtccttaa
    20461 attaaaaaac tcaaaagtaa tgactaatca tatttcatat tgttttttct ctatgtatgg
    20521 ctatacctat caatatttct taacttctaa gctcagaaat attgactatg acatcaaatc
    20581 gtttcgcaag ctctaaatca tgcgttacga caatcatagt tttattttca tcaagtagct
    20641 tcaataataa atcaataact atttttccat ttttcgtatc tagtgaccca gtaggttcat
    20701 cagctaaaat gattttcgga tcttttaata
//
