LOCUS       NZ_CP120019             3131 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog149 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120019
VERSION     NZ_CP120019.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696831
            Assembly: GCF_034043055.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the submitter's request
REFERENCE   3  (bases 1 to 3131)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 320x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:55:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,752
            Genes (coding)                    :: 2,665
            CDSs (with protein)               :: 2,665
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 46 of 87
            Pseudo Genes (incomplete)         :: 35 of 87
            Pseudo Genes (internal stop)      :: 21 of 87
            Pseudo Genes (multiple problems)  :: 15 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog149"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            90..278
                     /locus_tag="P3U14_RS14160"
                     /old_locus_tag="P3U14_14160"
     CDS             90..278
                     /locus_tag="P3U14_RS14160"
                     /old_locus_tag="P3U14_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(604..1185)
                     /locus_tag="P3U14_RS14165"
                     /old_locus_tag="P3U14_14165"
     CDS             complement(604..1185)
                     /locus_tag="P3U14_RS14165"
                     /old_locus_tag="P3U14_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            1637..2632
                     /locus_tag="P3U14_RS14170"
                     /old_locus_tag="P3U14_14170"
     CDS             1637..2632
                     /locus_tag="P3U14_RS14170"
                     /old_locus_tag="P3U14_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     Typing          2703..2867
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          1638..2632
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttaaaatac ccccttaaaa ctcaaaataa aggtatttat aatttgagta agtcttgtga
       61 taaagttttt taaaaaagga gtgtttattt tgaataaaaa cacagtgtta gatgaaggtt
      121 acgttatagc aactatttta aatgttttct ttttacttgg attaattttt atatctcatt
      181 tggaaaattt atatatatta attccgtatg ttattttgat gggaataaat gctatttatt
      241 tggttgttaa ggttatgaat ttcaaaaaga ataattagaa tatttgaggg gattttgaaa
      301 cgagtttctt cttgttttca cactgttttt ttattcctat tggtgttgtt gcttactttt
      361 tgtttttcta taaagatgat gcttttgacg agatggaaga aaaatatgat tatcatgaag
      421 ataataaaaa atagacgacg catttatgcc gagaaaattt attgatgttg agaagaaccc
      481 ttaactaaac ttgcagacga atgtcggcat agcgtgagct attaagccga ccatgcgaca
      541 agttttggga ttgttaaggg ttccgaggct caacgtcaat aaagcaattg gaataaatag
      601 tatctaaagt aacccgtatt taagattaca ttgtacatcc ttcgaaacca tagtagctgc
      661 gattccagct aggtttcctt ttatcccaat tttagctaat ttttttgcta cgccctttaa
      721 gtttttggct ttaacatctt caaaaactgc tgtgattatg tttccagtta aaagttctcc
      781 atatgagttt aaaacttcag aatagaaaca gtcaccgcta tttttgtagt tcccaccaac
      841 tgctctaggt gatattgaac tagttttctt tttattctct atgtcgtttt ttagtttttg
      901 gaatccttca ggaacatatc catatcgaga tttggcattt tctagattaa catctttagc
      961 tgttccgtct ttatctaaaa taataatttt gccatatatg tattttagtt ctttttcaat
     1021 ttctttttcg tttaaagttt caacgttttt atctgcaatg ttatttttgt tttgggcttg
     1081 agcttctttg aaattaaaat ccaagaccat tgaaagtatg gttaaaatac ttaaagatac
     1141 tactaaaatc tttcttagtg atatactgtt ttgaattgca ttcattgtaa atgccccctt
     1201 gttttgttat ttaaatgaat tcaatataat attaacatat aaaaacaaag gagtttaata
     1261 tgaataagtt tttttacttt ttgattattt tattatctgt atttttaatt tttaattttg
     1321 ttaattcgat tttatttgat aatccttttg actttttaga aagttttatt tattcattgg
     1381 ttttcacatt aatttattta ggtttagagg gtcataaaaa gaagaaggaa taggttgttt
     1441 tttgaaacga gtgtgaacga gtttcttctt gtcttgatac tatatagaaa taactcgatt
     1501 ttatatatat agctgtaact gttgatatta cagtgtttaa acgtgttttt gtgcgtgaaa
     1561 ggaaaatttg acaataaaaa accccagtta tattattaag gtgtcgaatc ttaaataata
     1621 ctgggggtct ttttatatgc aatataatac tactagaagt ataaccgaaa atcaagataa
     1681 taaaacgtta aaagatatga cgaaaagtgg gaaacaacgc ccatggagag aaaagaaaat
     1741 agataatgta agctatgcag atatactaga aattttaaaa atcaaaaagg cttttaatgt
     1801 aaaacaatgt ggtaatattt tagaatttaa gccaactgat gaaggctatt tgaagttaca
     1861 taagacatgg ttttgtaaat caaaattatg tccggtttgt aattggagac gtgctatgaa
     1921 aaatagttat caagctcaaa aagtgattga aaaagtaatt aaggaaaagc caaaagcacg
     1981 ttggttgttt ttaacacttt caacaaaaaa tgcgatagat ggagatactt tagaacaaag
     2041 tttgaagcat ctaactaaag catttgatag gttgagtaga tataaaaagg ttaaacaaaa
     2101 tcttgttgga tttatgcgtt caacagaagt taccgttaat aaaaatgacg gtagttataa
     2161 tcagcacatg catgttttgt tatgtgttga aaatgcatat tttagaaaaa aagagaatta
     2221 tataactcaa gaagaatggg ttaatttatg gcaaagagca ttacaagttg actatcgacc
     2281 tgttgctaat gttaaagcga tcaaaccgaa tagaaaaggc gataaagaca ttgaatcggc
     2341 aatcaaagag acctcaaaat attcggttaa atcatctgat tttttaactg atgatgatga
     2401 aaaaaatcaa gaaattgtaa gtgatttaga aaaaggtttg tatcgaaaac gtatgttaag
     2461 ttatggtgga ttgcttaaac aaaaacataa aattttaaac ttagacgatg tcgaagatgg
     2521 taatttgatt aatgcaagtg atgaagataa aacaacagac gaagaagaaa aagcacattc
     2581 aattaccgca atttggaatt tcgaaaagca aaattattat ttaagacatt agtgttgact
     2641 aatgtctttt ttgttgattt tttataaaaa agtactgtct tatttttgtg acaaatgctg
     2701 tatgtagtgt cacaaaaata agacaaacgc aatatattgt gtcacaaaaa taagacagta
     2761 cagctttgta tgatatcact ttaaaatacc ttaaaacccc tggaatgtct ggctttgcca
     2821 gacctatcat ttttgaatga tagcaaattc tccttatgct cttacggagt ttttagagaa
     2881 aaattaaaaa ttctcgattt ttgataaaaa acgccctgca ggaatttaga aaaacatgtg
     2941 gaagttttaa aggattttat gctaattttt aatttgcatg taaatgggca gtgttaaaaa
     3001 attagtcact ggttttgctg aaatttttgt ttgtgaaatt accatatatt tatttggctc
     3061 atatttgcgt tttaagagct tgtatatatt tttaagttaa aaacctatat gatacccccc
     3121 taaaccttta a
//
