LOCUS       NZ_CP120016             4439 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog152 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120016
VERSION     NZ_CP120016.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696832
            Assembly: GCF_034043035.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the submitter's request
REFERENCE   3  (bases 1 to 4439)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 662x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 11:15:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,737
            CDSs (total)                      :: 2,655
            Genes (coding)                    :: 2,588
            CDSs (with protein)               :: 2,588
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 33 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 18 of 67
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog152"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            complement(142..1383)
                     /gene="mobV"
                     /locus_tag="P3U01_RS13675"
                     /old_locus_tag="P3U01_13675"
     CDS             complement(142..1383)
                     /gene="mobV"
                     /locus_tag="P3U01_RS13675"
                     /old_locus_tag="P3U01_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000122374.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     gene            complement(1569..2948)
                     /gene="tet(K)"
                     /locus_tag="P3U01_RS13680"
                     /old_locus_tag="P3U01_13680"
     ARG             complement(1573..2948)
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3110..4054)
                     /locus_tag="P3U01_RS13685"
                     /old_locus_tag="P3U01_13685"
     CDS             complement(3110..4054)
                     /locus_tag="P3U01_RS13685"
                     /old_locus_tag="P3U01_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(3111..4054)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(146..1383)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggaacctac agggctttat gtgttgttga gttggtactt tcttgggatt aatcccaatt
       61 caagtccaac caactcgcta acaagttagc taacacatag cccattccaa ccaataagtt
      121 ttctcggcat aaatgcatgt tctataagtg catacctcta tctcgttttt gattcttttg
      181 ttgttcttgt tgctgttcct gttctgaata taattctgga ttcatttttt tatctaagtt
      241 tcctgctaaa ccatttagtt ttggttcatc ttctgttaac ttattaactc tttccgcaaa
      301 ggtatttctt cctaaaactc gttctaactc ttttagtaag atttttaaaa gctttaacgc
      361 tatttctata ttctctttaa gtggctttgc attttcgtaa agttggttaa aattatcgtc
      421 tgcgttttca atacgctcaa ctgctttatt taataaatca tctttctctc gtatttcctt
      481 atctttatca tctaaggctc taccagactt tatatactcg taatcttccg aaatatcttg
      541 agcagctttt atctgtttct gaaattcatt gaaatctttt tggcttatta caacatttcc
      601 agtttcttgt atttctttgc taaataaacc acctactttt tcagtttctt gctcatacgg
      661 aacatttata ggctttttaa gcgtatttaa cgatttttgg tactcttgca ttaatttatc
      721 gttcttttgc tttatatggt ctgttttttg gctctcacgt tcatattctt gcttatgata
      781 ttctgttttt tgtttatact gacttatttg ctcatgttta gcatttgtta cttgtcttga
      841 ttgcccacgt tctaaatcat atcctcgttg tttaacatgc tcattaaatc tatcttgaaa
      901 cgctgttaaa gcttttttat tacctacaac ttctttagca cttaaacgac catcatcagt
      961 tattggaaca acgccataat gcatatgtgg tgttttttcg tccatgtgaa ctgttgcata
     1021 taataaatta tctttaccgt attcttgttc taaaaactct ttagcatatt caaaaaactg
     1081 ctttgtatct tctggcgttt gattatcaaa gaaatcattg tctgatgtaa ttaaaccatc
     1141 aatgtgttta atcgcgtctg ttctaatttt tcttttgcct gtataattct gttcgatttt
     1201 ttcatcaatc aagttattaa aattctgttt attagcattt accaaatcat aatttaagta
     1261 agttttacta tggtctatat cttcattttc ataattatta ttttctcttt gaacatgttt
     1321 ttgtatgccc gttgtatttg ttccagattt aacttttgaa actctaacaa tggaataaga
     1381 catataaaaa tcactcctaa gcacaaaggt ttcatgtgta tttaatagtt taagtctaac
     1441 agactagact tatttttaat aagtcgttaa tacgtgtgct ctgcgaggct taaacctgtt
     1501 tttgctaacg caaaaaatga gtggcaaaat gctagccact catagttcta aaccaaaata
     1561 taatataact attcaaactg cttttcagaa cgtttaaata caataatcgt caaaagacaa
     1621 cataaaataa taaggatagc catggctaca agaatattac tatacactcc agaagaataa
     1681 tttataaatt ccagaactag tttacgatta atcaattgta gtgacaataa acctcctaca
     1741 attgctatac ctgttccctc tgataaaaaa cttgtgaaat ttagcaaact cattccagaa
     1801 gcaacttctt cttcagaaag actacttgat actatttttg atataactgt tttagtaaaa
     1861 gataatccgc ccataacaaa tataaacata aaagtagtca accacatact aaactcaaca
     1921 aaaaatgcaa tagttaaaaa acttatagag atagacaatg atcctaaaat aaaaacaaat
     1981 aatgatcctt ttctatccac taaaaaacca ccaaaataac caaaaacaat aacactcatg
     2041 gttccaggaa aaataacact attacctatt gtcgctacat ttacatgata aatagttttc
     2101 atcatataag gcaccattga tataaaacca gctactatag aaaatattag cccaccagaa
     2161 aacaaaccaa gcataaacgg aatgtttttc cctagtttag gattaataaa agggttagaa
     2221 actcttgaaa tatgtttaat aaaaatcaca aaaaagattg tgaagagtat taaaaaagtc
     2281 caattataat ttgtcgtaaa taacataaaa catataatac ttatagacat taaaacaata
     2341 cctacgatat ctaatgtatt ttttgttgat ttaccaggta ccattacttt aataagaaaa
     2401 ggtatagtta ctattgtaat cataggaagt ataagtaggt aagaccaatg aatataatgt
     2461 gctattattc cccctattga aggacctaac ccttcaccta aagctacaat tgatcctata
     2521 aaaccaaagg ctttgccttg tttttttctt gtaatatttc tagctacaac caccataatc
     2581 agtgaaggga atgcagcaga tcctactcct tgtactaacc taccaaaaat caaaataaaa
     2641 aagtgattgt gaccaataaa agcaatcaat gaaccaagac agctcaaact aataccaata
     2701 attaacaatt tttttatatt tatataatca gataattttc catatactgc tgttcctatc
     2761 gaaaaagtta acatatatgc agtgtttacc cagtttgtaa ttccaggagt agtattaaaa
     2821 tgatttgcaa tatcaggtaa agaaacattt aaaaccattt catttaatac actaaaaaat
     2881 gaaagaatac aaagccaaaa taaaacgcta taaaacaaac ctttaaattt tttatataaa
     2941 ctaaacaatt ttacctcttt acttgaggtg actaaagttt ataggggtaa attattcttt
     3001 cacctcatat aaattcccca aatttttaaa tagacacttc ataaaaaaat cctcctaaat
     3061 tagaatagtt taaatatatc atttttgttc agtaatatta atatgtacac tatttccaaa
     3121 atttaaattc atgttgccaa aaatcgattt gtttttgcaa ttgtttttcg ttcgctttta
     3181 aagtcgattt cattaagtcc gttaaatcga ctggcgaaat ttcttttatc aaattcttat
     3241 attttgttct agaatttctg tgaagctttc cccattcttc ttcatcactc aataacataa
     3301 aaactattgc tctatccgca gtgcgttgga tagttttcca atctggttgc aagatatgta
     3361 aatcactaaa gcaatcattc cagtaatcca ccatatctct tttaagttcg atttctacac
     3421 gccataaatg ttcagacata acttcagcat ctgcattatc tttacgttct tgctttttat
     3481 tataaattct aataaatcta ttactatctc tcacgccaaa atattttgtt tctggcttac
     3541 cattacgacc ataaaaaata gttttcttaa ctgctttatc agacattgca tagtagtcac
     3601 tcaaatcatc ttcaaaatca aaggctaaat ctaatcttgt aaaaccgtca tcttccatgt
     3661 agcttattat attttgtttt aaccaaatca tttcatctcg tgtaagtttg tttggattaa
     3721 attcaatacg catattacgt ctatcccaac tatctgcttt cactttgtca tattcaatat
     3781 aaactttttc ttgaagtgct ttagctttaa actttgtttg aagaatatcc caaagtctta
     3841 tttggggctc tacactcata aatttagaaa gggcttgagc gttgtctcgg ttgagatttc
     3901 caacgatcgt cattgcatca aaacttaatt ttggatcaga tatgcacaca gtatgtgcgt
     3961 ccaaccggct attagagtag ccggttttag aaaaattgtc taaatcgtga ttttccaaat
     4021 gatttgaatg atttgcatga ttgtttttat acataaaaaa tcgactcctt aatctcaatt
     4081 tcgtttaagg aatcgctcac ccaaatatat atcttgatgt atatttaaat atcgtttaat
     4141 atctaaatat acaagattat aaaaataact cagtgttttt ttctttgaat gatgtcgttc
     4201 acaaactttg gtcagggcgt gagcgactcc tttttatttt gttattaata taacactatc
     4261 aaaagacttg gtctaatcag atcaagtctt tttttattta agcatttgta ttatctggta
     4321 aacagttaaa actactaaaa caccaagtac atacttactt gttataaaat tctcaaaggc
     4381 ttctctgaat ttcatttctt caactccttt tattgcttta ttccaatttc cttattggt
//
