LOCUS       NZ_CP120006             4441 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog162 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120006
VERSION     NZ_CP120006.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696836
            Assembly: GCF_034042955.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the request of the submitter
REFERENCE   3  (bases 1 to 4441)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 941x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:59:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,738
            CDSs (total)                      :: 2,656
            Genes (coding)                    :: 2,588
            CDSs (with protein)               :: 2,588
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 34 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 18 of 68
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog162"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            complement(join(3605..4441,1..407))
                     /gene="mobV"
                     /locus_tag="P3U22_RS13680"
                     /old_locus_tag="P3U22_13680"
                     /pseudo=""
     CDS             complement(join(3605..4441,1..407))
                     /gene="mobV"
                     /locus_tag="P3U22_RS13680"
                     /old_locus_tag="P3U22_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            complement(593..1972)
                     /gene="tet(K)"
                     /locus_tag="P3U22_RS13685"
                     /old_locus_tag="P3U22_13685"
     ARG             complement(597..1972)
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2134..3078)
                     /locus_tag="P3U22_RS13690"
                     /old_locus_tag="P3U22_13690"
     CDS             complement(2134..3078)
                     /locus_tag="P3U22_RS13690"
                     /old_locus_tag="P3U22_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(2135..3078)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..407)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.739999999999997e-82"
     Typing          complement(3609..4441)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.23e-147"
ORIGIN
        1 taatgcatat gtggtgtttt ttcgtccatg tgaactgttg catataataa attatcttta
       61 ccgtattctt gttctaaaaa ctctttagca tattcaaaaa actgctttgt atcttctggc
      121 gtttgattat caaagaaatc attgtctgat gtaattaaac catcaatgtg tttaatcgcg
      181 tctgttctaa tttttctttt gcctgtataa ttctgttcga ttttttcatc aatcaagtta
      241 ttaaaattct gtttattagc atttaccaaa tcataattta agtaagtttt actatggtct
      301 atatcttcat tttcataatt attattttct ctttgaacat gtttttgtat gcccgttgta
      361 tttgttccag atttaacttt tgaaactcta acaatggaat aagacatata aaaatcactc
      421 ctaagcacaa aggtttcatg tgtatttaat agtttaagtc taacagacta gacttatttt
      481 taataagtcg ttaatacgtg tgctctgcga ggcttaaacc tgtttttgct aacgcaaaaa
      541 atgagtggca aaatgctagc cactcatagt tctaaaccaa aatataatat aactattcaa
      601 actgcttttc agaacgttta aatacaataa tcgtcaaaag acaacataaa ataataagga
      661 tagccatggc tacaagaata ttactataca ctccagaaga ataatttata aattccagaa
      721 ctagtttacg attaatcaat tgtagtgaca ataaacctcc tacaattgct atacctgttc
      781 cctctgataa aaaacttgtg aaatttagca aactcattcc agaagcaact tcttcttcag
      841 aaagactact tgatactatt tttgatataa ctgttttagt aaaagataat ccgcccataa
      901 caaatataaa cataaaagta gtcaaccaca tactaaactc aacaaaaaat gcaatagtta
      961 aaaaacttat agagatagac aatgatccta aaataaaaac aaataatgat ccttttctat
     1021 ccactaaaaa accaccaaaa taaccaaaaa caataacact catggttcca ggaaaaataa
     1081 cactattacc tattgtcgct acatttacat gataaatagt tttcatcata taaggcacca
     1141 ttgatataaa accagctact atagaaaata ttagcccacc agaaaacaaa ccaagcataa
     1201 acggaatgtt tttccctagt ttaggattaa taaaagggtt agaaactctt gaaatatgtt
     1261 taataaaaat cacaaaaaag attgtgaaga gtattaaaaa agtccaatta taatttgtcg
     1321 taaataacat aaaacatata atacttatag acattaaaac aatacctacg atatctaatg
     1381 tattttttgt tgatttacca ggtaccatta ctttaataag aaaaggtata gttactattg
     1441 taatcatagg aagtataagt aggtaagacc aatgaatata atgtgctatt attcccccta
     1501 ttgaaggacc taacccttca cctaaagcta caattgatcc tataaaacca aaggctttgc
     1561 cttgtttttt tcttgtaata tttctagcta caaccaccat aatcagtgaa gggaatgcag
     1621 cagatcctac tccttgtact aacctaccaa aaatcaaaat aaaaaagtga ttgtgaccaa
     1681 taaaagcaat caatgaacca agacagctca aactaatacc aataattaac aattttttta
     1741 tatttatata atcagataat tttccatata ctgctgttcc tatcgaaaaa gttaacatat
     1801 atgcagtgtt tacccagttt gtaattccag gagtagtatt aaaatgattt gcaatatcag
     1861 gtaaagaaac atttaaaacc atttcattta atacactaaa aaatgaaaga atacaaagcc
     1921 aaaataaaac gctataaaac aaacctttaa attttttata taaactaaac aattttacct
     1981 ctttacttga ggtgactaaa gtttataggg gtaaattatt ctttcacctc atataaattc
     2041 cccaaatttt taaatagaca cttcataaaa aaatcctcct aaattagaat agtttaaata
     2101 tatcattttt gttcagtaat attaatatgt acactatttc caaaatttaa attcatgttg
     2161 ccaaaaatcg atttgttttt gcaattgttt ttcgttcgct tttaaagtcg atttcattaa
     2221 gtccgttaaa tcgactggcg aaatttcttt tatcaaattc ttatattttg ttctagaatt
     2281 tctgtgaagc tttccccatt cttcttcatc actcaataac ataaaaacta ttgctctatc
     2341 cgcagtgcgt tggatagttt tccaatctgg ttgcaagata tgtaaatcac taaagcaatc
     2401 attccagtaa tccaccatat ctcttttaag ttcgatttct acacgccata aatgttcaga
     2461 cataacttca gcatctgcat tatctttacg ttcttgcttt ttattataaa ttctaataaa
     2521 tctattacta tctctcacgc caaaatattt tgtttctggc ttaccattac gaccataaaa
     2581 aatagttttc ttaactgctt tatcagacat tgcatagtag tcactcaaat catcttcaaa
     2641 atcaaaggct aaatctaatc ttgtaaaacc gtcatcttcc atgtagctta ttatattttg
     2701 ttttaaccaa atcatttcat ctcgtgtaag tttgtttgga ttaaattcaa tacgcatatt
     2761 acgtctatcc caactatctg ctttcacttt gtcatattca atataaactt tttcttgaag
     2821 tgctttagct ttaaactttg tttgaagaat atcccaaagt cttatttggg gctctacact
     2881 cataaattta gaaagggctt gagcgttgtc tcggttgaga tttccaacga tcgtcattgc
     2941 atcaaaactt aattttggat cagatatgca cacagtatgt gcgtccaacc ggctattaga
     3001 gtagccggtt ttagaaaaat tgtctaaatc gtgattttcc aaatgatttg aatgatttgc
     3061 atgattgttt ttatacataa aaaatcgact ccttaatctc aatttcgttt aaggaatcgc
     3121 tcacccaaat atatatcttg atgtatattt aaatatcgtt taatatctaa atatacaaga
     3181 ttataaaaat aactcagtgt ttttttcttt gaatgatgtc gttcacaaac tttggtcagg
     3241 gcgtgagcga ctccttttta ttttgttatt aatataacac tatcaaaaga cttggtctaa
     3301 tcagatcaag tcttttttta tttaagcatt tgtattatct ggtaaacagt taaaactact
     3361 aaaacaccaa gtacatactt acttgttata aaattctcaa aggcttctct gaatttcatt
     3421 tcttcaactc cttttattgc tttattccaa tttccttatt ggtcggaacc tacagggctt
     3481 tatgtgttgt tgagttggta ctttcttggg attaatccca attcaagtcc aaccaactcg
     3541 ctaacaagtt agctaacaca tagcccattc caaccaataa gttttctcgg cataaatgca
     3601 tgttctataa gtgcatacct ctatctcgtt tttgattctt ttgttgttct tgttgctgtt
     3661 cctgttctga atataattct ggattcattt ttttatctaa gtttcctgct aaaccattta
     3721 gttttggttc atcttctgtt aacttattaa ctctttccgc aaaggtattt cttcctaaaa
     3781 ctcgttctaa ctcttttagt aagattttta aaagctttaa cgctatttct atattctctt
     3841 taagtggctt tgcattttcg taaagttggt taaaattatc gtctgcgttt tcaatacgct
     3901 caactgcttt atttaataaa tcatctttct ctcgtatttc cttatcttta tcatctaagg
     3961 ctctaccaga ctttatatac tcgtaatctt ccgaaatatc ttgagcagct tttatctgtt
     4021 tctgaaattc attgaaatct ttttggctta ttacaacatt tccagtttct tgtatttctt
     4081 tgctaaataa accacctact ttttcagttt cttgctcata cggaacattt ataggctttt
     4141 taagcgtatt taacgatttt tggtactctt gcattaattt atcgttcttt tgctttatat
     4201 ggtctgtttt ttggctctca cgttcatatt cttgcttatg atattctgtt ttttgtttat
     4261 actgacttat ttgctcatgt ttagcatttg ttacttgtct tgattgccca cgttctaaat
     4321 catatcctcg ttgtttaaca tgctcattaa atctatcttg aaacgctgtt aaagcttttt
     4381 tattacctac aacttcttta gcacttaaac gaccatcatc agttattgga acaacgccat
     4441 a
//
