LOCUS       NZ_CP119977            12808 bp    DNA     circular CON 19-MAY-2024
DEFINITION  Enterobacter kobei strain MUMC-2 plasmid pMUMC-2_4, complete
            sequence.
ACCESSION   NZ_CP119977
VERSION     NZ_CP119977.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33608023
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 12808)
  AUTHORS   Hendrickx,A.P.A.
  TITLE     Mobile colistin resistance mcr-4.3- and mcr-4.6-harbouring plasmids
            in livestock- and human-retrieved Enterobacterales in the
            Netherlands
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12808)
  AUTHORS   Hendrickx,A.P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2023) Bacterial Surveillance and Response,
            National Institute for Public Health and the Environment, Antoni van
            Leeuwenhoeklaan 9, Bilthoven 3721 MA, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Partial
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029338095.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 04:47:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 14
            CDSs (total)                      :: 14
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119977.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12808
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="MUMC-2"
                     /host="Homo sapiens"
                     /db_xref="taxon:208224"
                     /plasmid="pMUMC-2_4"
                     /geo_loc_name="Netherlands"
     gene            1..>786
                     /locus_tag="P2D90_RS00005"
                     /old_locus_tag="P2D90_00005"
                     /pseudo=""
     CDS             1..>786
                     /locus_tag="P2D90_RS00005"
                     /old_locus_tag="P2D90_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            complement(1080..2102)
                     /locus_tag="P2D90_RS00010"
                     /old_locus_tag="P2D90_00010"
     CDS             complement(1080..2102)
                     /locus_tag="P2D90_RS00010"
                     /old_locus_tag="P2D90_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="WP_000156887.1"
                     /translation="MTITTVGIDLAKNVFAVHGVDQNGRTVLVKPKVSRAALPELIASL
                     PPCVIGMEACSGAHYWARLFRQYGHEPRLMAAKFVSPYRMAGKSGKNDAADAQAICEAV
                     RRPHMRFVPVKDESQQAMQCLHRTRQGFIEEKTATYNRLRGLISEFGVIAPQSADALRH
                     MVSVQKSSLPLQVQQCVDDLMEHVDRIEANITEYDRILSHIAKTDHRSQRLMELKGVGP
                     TTACALVASIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDSYLRTLLVQG
                     ARSVLIGAGKRTDSFSRWVCMLVERRGYWRAVVAIAAKNARLCWASLHYGDDFRLYSVN
                     "
                     /name="IS110 family transposase"
     gene            2462..3484
                     /locus_tag="P2D90_RS00015"
                     /old_locus_tag="P2D90_00015"
     CDS             2462..3484
                     /locus_tag="P2D90_RS00015"
                     /old_locus_tag="P2D90_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="WP_000156887.1"
                     /translation="MTITTVGIDLAKNVFAVHGVDQNGRTVLVKPKVSRAALPELIASL
                     PPCVIGMEACSGAHYWARLFRQYGHEPRLMAAKFVSPYRMAGKSGKNDAADAQAICEAV
                     RRPHMRFVPVKDESQQAMQCLHRTRQGFIEEKTATYNRLRGLISEFGVIAPQSADALRH
                     MVSVQKSSLPLQVQQCVDDLMEHVDRIEANITEYDRILSHIAKTDHRSQRLMELKGVGP
                     TTACALVASIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDSYLRTLLVQG
                     ARSVLIGAGKRTDSFSRWVCMLVERRGYWRAVVAIAAKNARLCWASLHYGDDFRLYSVN
                     "
                     /name="IS110 family transposase"
     gene            complement(3818..4402)
                     /locus_tag="P2D90_RS00020"
                     /old_locus_tag="P2D90_00020"
     CDS             complement(3818..4402)
                     /locus_tag="P2D90_RS00020"
                     /old_locus_tag="P2D90_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_040113348.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLSR
                     LIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVMAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRATYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(4415..5512)
                     /gene="mobA"
                     /locus_tag="P2D90_RS00025"
                     /old_locus_tag="P2D90_00025"
     CDS             complement(4415..5512)
                     /gene="mobA"
                     /locus_tag="P2D90_RS00025"
                     /old_locus_tag="P2D90_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_040113349.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGNM
                     PAWAAHKPTEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKAPERGGAKKARFG
                     ETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIEREPERHLGAGQVQR
                     FDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQE
                     DTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMERLAEKQR
                     QQEKEQQRLAEQTRQKPDKGWSFSR"
                     /name="mobA"
     gene            5678..6145
                     /gene="traD"
                     /locus_tag="P2D90_RS00030"
                     /old_locus_tag="P2D90_00030"
     CDS             5678..6145
                     /gene="traD"
                     /locus_tag="P2D90_RS00030"
                     /old_locus_tag="P2D90_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_032235402.1"
                     /translation="MSKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     gene            6738..7688
                     /locus_tag="P2D90_RS00035"
                     /old_locus_tag="P2D90_00035"
     CDS             6738..7688
                     /locus_tag="P2D90_RS00035"
                     /old_locus_tag="P2D90_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_034167667.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSHLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     gene            7696..8109
                     /locus_tag="P2D90_RS00040"
                     /old_locus_tag="P2D90_00040"
     CDS             7696..8109
                     /locus_tag="P2D90_RS00040"
                     /old_locus_tag="P2D90_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001229901.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            8356..8673
                     /locus_tag="P2D90_RS00045"
                     /old_locus_tag="P2D90_00045"
     CDS             8356..8673
                     /locus_tag="P2D90_RS00045"
                     /old_locus_tag="P2D90_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_040113353.1"
                     /translation="MFNVILHDDVPNELAGLPPVVRAKMIRLIDKLKTNATALREPDSK
                     PLRDGLFELRTMGTDIARGLYVYQKGRNIYLLRVFIKKTQKTPSGEISMAFERLKEMLN
                     E"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            8666..8953
                     /locus_tag="P2D90_RS00050"
                     /old_locus_tag="P2D90_00050"
     CDS             8666..8953
                     /locus_tag="P2D90_RS00050"
                     /old_locus_tag="P2D90_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104592.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_172691904.1"
                     /translation="MSKVKGIAWEHVRNELMSNPEVQAAYEAEERKERLQAMLAEWRHH
                     AGLTRAQVAERMGVTPPTVSRMESNVIKASIETLARYARACGVKHPQITL"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(9291..10916)
                     /locus_tag="P2D90_RS00055"
                     /old_locus_tag="P2D90_00055"
     CDS             complement(9291..10916)
                     /locus_tag="P2D90_RS00055"
                     /old_locus_tag="P2D90_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006081694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-4.3"
                     /protein_id="WP_011638903.1"
                     /translation="MISRFKTLSVNQFTFITALFYVAIFNLPLFGIVRKGIEKQPEVDP
                     LFIASMPLFLTFALSFLFSIFTVKYLLKPFFIVLTLLSSSVFFAAYQYNVVFDYGMIEN
                     TFQTHPAEALMYVNLASITNLLLTGLLPSYLIYKADIHYQPFFKELLHKLAFMLLMFVG
                     IGIVAFFYYQDYAAFGRNNSELRRYIVPTYFVSSASKYLNEHYLQTPMEYQQLGLDAKN
                     ASRNPNTKPNLLVFVVGETARSMSYQYYGYNKPTNAHTQNQGLIAFNDTSSCGTATAVS
                     LPCMFSRMGRADYDPRRANAQDTVIDVLSHSGIKVQWFDNDSGCKGVCDQVENLTIDLK
                     SDPKLCSGQYCFDQVLLNKLDKILAVAPSQDTVIFLHIIGSHGPTYYLRYPPEHRKFIP
                     DCPRSDIQNCSQEELINTYDNTILYTDFILSEVVNKLKGKQDMFDTAMLYLSDHGESLG
                     EKGMYLHGAPYSIAPKEQTSVPMLAWVSNDFSQDNQLNMTCVAQRAEQGGFSHDNLFDS
                     LLGLMNVKTTVYQSQLDIFAPCRY"
                     /name="phosphoethanolamine--lipid A transferase MCR-4.3"
     gene            complement(11090..>11260)
                     /locus_tag="P2D90_RS00060"
                     /old_locus_tag="P2D90_00060"
                     /pseudo=""
     CDS             complement(11090..>11260)
                     /locus_tag="P2D90_RS00060"
                     /old_locus_tag="P2D90_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            11322..12026
                     /locus_tag="P2D90_RS00065"
                     /old_locus_tag="P2D90_00065"
     CDS             11322..12026
                     /locus_tag="P2D90_RS00065"
                     /old_locus_tag="P2D90_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <12195..12512
                     /locus_tag="P2D90_RS00070"
                     /old_locus_tag="P2D90_00070"
                     /pseudo=""
     CDS             <12195..12512
                     /locus_tag="P2D90_RS00070"
                     /old_locus_tag="P2D90_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016707037.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /name="tyrosine-type recombinase/integrase"
     ARG             complement(9295..10916)
                     /name="mcr-4.3"
                     /gene="mcr-4.3"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-4.3"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          7513..7688
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(4419..5512)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagccatc aactcacctt cgccgatagt gaattcagca ctaagcgccg tcagacccga
       61 aaagagattt tcctctcccg catggagcag attctgccat ggcagaatat gaccgctgtc
      121 atcgagccgt tttatcccaa ggcgggcaat ggccgacggc cctatccgct ggagaccatg
      181 ctacgcattc actgcatgca gcattggtac aacctgagcg atggcgcgat ggaagatgct
      241 ctgtacgaaa tcgcctccat gcgtctgttt gcccggttat ccctggatag cgccttgccg
      301 gaccgcacca ccatcatgaa tttccgccac ctgctggagc agcatcaact ggcccgccaa
      361 ttgttcaaga ccatcaatcg ctggctggcc gaagcaggcg tcatgatgac tcaaggcacc
      421 ttggtcgatg ccaccatcat tgaggcaccc agctcgacca agaacaaaga gcagcaacgc
      481 gatccggaga tgcatcagac caagaaaggc aatcagtggc actttggcat gaaggcccac
      541 attggtgtcg atgccaagag tggcctgacc cacagcctgg tcaccaccgc ggccaacgag
      601 catgacctca atcagctggg taatctgctg catggagagg agcaatttgt ctcagccgat
      661 gccggctacc aaggggcgcc acagcgcgag gagctggccg aggtggatgt ggactggctg
      721 atcgccgagc gccccggcaa ggtaagaacc ttgaaacagc atccacgcaa gaacaaaacg
      781 gccatcaagg ggtcagtttg gagatcggaa atttttgtac tataaggact tccccggcat
      841 gtcaaagccg gtatttatgg tttctgtctg ctcttcagta aaaataaagg ggagagactg
      901 cggtactata aacggccatg atgaatcgtc atcgattcgg gccctgatga aagacgcgcg
      961 tgaggttctt gttcgttaag cggatacgta tatcctgtac aatagccaga taggcctcac
     1021 ggggtctaac cctttatcat caacgaattg caggtcagat ggttatacag cttcgagctt
     1081 tagttgactg agtacagccg gaaatcatca ccgtaatgta gtgatgccca acacagccgc
     1141 gcatttttgg cggcgatggc cacaacagcc cgccagtacc ctctgcgctc aaccaacata
     1201 caaacccaac ggctgaatga gtcagtcctt ttcccggcgc caataagcac tgaacgagcc
     1261 ccctgaacca gaagcgttcg cagatacgaa tcaccggctt ttgtgatcct gccgagtttc
     1321 gactttcccc cgctgctgta ttgtgatggc gtcagcccca gccaggctgc cagttgacgt
     1381 ccatttttaa aatcatgtgc attaccgata ctggcgacca gtgcacaggc cgttgtggga
     1441 ccaacgccct ttagctccat cagccgctga ctgcgatgat ctgttttggc tatgtgggac
     1501 aaaattcggt catattcagt gatgttagct tcaatgcgat caacgtgttc catcaaatca
     1561 tcaacacatt gctgaacctg aagcggtaaa gagctcttct gcacagaaac catgtggcgc
     1621 agggcatcag cactttgtgg cgcgatgaca ccgaattcag atatcaatcc tcgcagacga
     1681 ttatacgttg ctgttttctc ttcgataaaa ccctgacggg tacgatgtaa acactgcatg
     1741 gcctgctggc tttcgtcttt cactggtacg aaccgcatat gcggacgacg aacagcttca
     1801 cagatagcct gagcatcagc tgcatcattt tttcctgatt ttccggccat acgataaggc
     1861 gatacaaact tagcagccat cagacgtggc tcatgaccat actgccgaaa caatcttgcc
     1921 cagtagtgag ccccggagca tgcttccatc ccgataacac agggaggcaa acttgcaatt
     1981 aactcaggaa gtgcagcacg cgatacttta ggcttaacca gaacagttct accattttga
     2041 tcaacaccgt gaacagcaaa cacattttta gcaagatcga taccgacagt cgtgatagtc
     2101 ataacgaatt cctccgggac gatgtgtacc ccatgattgc acaagagtta atcaggcgca
     2161 taatcagggg aagtcccttc cattcgttaa gcacattatt taaccaattc ggatacgtaa
     2221 gtgattgatt tatcgtcagt ggctatcgct tttaggttgt cgctcttgct gtatcgcatt
     2281 taggttatcc gattaagaca aaatcccctg aaaacgctgt atagcgcggg agtacaccct
     2341 attgctatag gtaagtctgt tcaaaaaaca ggcttaccga atggaaggga cttcccctga
     2401 ttatgcgcct gattaactct tgtgcaatca tggggtacac atcgtcccgg aggaattcgt
     2461 tatgactatc acgactgtcg gtatcgatct tgctaaaaat gtgtttgctg ttcacggtgt
     2521 tgatcaaaat ggtagaactg ttctggttaa gcctaaagta tcgcgtgctg cacttcctga
     2581 gttaattgca agtttgcctc cctgtgttat cgggatggaa gcatgctccg gggctcacta
     2641 ctgggcaaga ttgtttcggc agtatggtca tgagccacgt ctgatggctg ctaagtttgt
     2701 atcgccttat cgtatggccg gaaaatcagg aaaaaatgat gcagctgatg ctcaggctat
     2761 ctgtgaagct gttcgtcgtc cgcatatgcg gttcgtacca gtgaaagacg aaagccagca
     2821 ggccatgcag tgtttacatc gtacccgtca gggttttatc gaagagaaaa cagcaacgta
     2881 taatcgtctg cgaggattga tatctgaatt cggtgtcatc gcgccacaaa gtgctgatgc
     2941 cctgcgccac atggtttctg tgcagaagag ctctttaccg cttcaggttc agcaatgtgt
     3001 tgatgatttg atggaacacg ttgatcgcat tgaagctaac atcactgaat atgaccgaat
     3061 tttgtcccac atagccaaaa cagatcatcg cagtcagcgg ctgatggagc taaagggcgt
     3121 tggtcccaca acggcctgtg cactggtcgc cagtatcggt aatgcacatg attttaaaaa
     3181 tggacgtcaa ctggcagcct ggctggggct gacgccatca caatacagca gcgggggaaa
     3241 gtcgaaactc ggcaggatca caaaagccgg tgattcgtat ctgcgaacgc ttctggttca
     3301 gggggctcgt tcagtgctta ttggcgccgg gaaaaggact gactcattca gccgttgggt
     3361 ttgtatgttg gttgagcgca gagggtactg gcgggctgtt gtggccatcg ccgccaaaaa
     3421 tgcgcggctg tgttgggcat cactacatta cggtgatgat ttccggctgt actcagtcaa
     3481 ctaaagctcg aagctgtata accatctgac ctgcaattcg ttgatgataa agggttagac
     3541 cccgtgaggc ctatctggct attgtacagg atatacgtat ccgcttaacg aacaagaacc
     3601 tcacgcgcgt ctttcatcag ggcccgaatc gatgacgatt catcatggcc gtttatagta
     3661 ccgcagtctc tcccctttat ttttactgaa gagcagacag aaaccataaa taccggcttt
     3721 gacatgccgg ggaagtcctt atagtacaat aattctctat atccaaactg accccctaat
     3781 tcacgacaac aataacggcg caatgtgcgc cgttatgtta ttttttgata atcatccagg
     3841 tattctcttt atcaccatac gtagctcggg tgtccacctt aacgcagggc tttccgtcac
     3901 aacgcgatat atcagccagc ggggctttct tctgccagac cgcttccatc cgttgcattt
     3961 cagcaatctg gctgtacccg tcattcataa accacgtaaa tgccgtcacg caggcagcca
     4021 tgacgaaaaa tatcgtcagc acgagataaa tcgcggattt ccacgtatag cgcgacatct
     4081 cgcgtcgcat ttcatggatc atcgccttcg ccgtatcggt agcctgatta agcgcttctg
     4141 tcgccggttt ctgctgtgct aatccggctt gtttcagctc tttctcaacc tgaatgagcg
     4201 cggcatgaac agattgcctg acggtttcag tcatattacc ggacgcgctg tccagctcac
     4261 gaatgagcct gctcagcgtg tcactctgct gctgtaattg tgctgaggcg tcctgaaact
     4321 gctcagtcag agaggtaaca cgcttttcca gcgcctgatg atgcccgatg agggcagcga
     4381 tttgtttaat ttcgtcgctc atgaaaaatc ctgtctatcg tgaaaatgac cagcctttat
     4441 ccggcttctg tcgtgtctgt tcggcgagtc tctgctgttc tttctcctgc tgacgctgtt
     4501 tttctgccag tcgctccatt tcctgatgta tcccctgaaa ctccgccatt gtctcgttca
     4561 caagggactg aaggtcgatt tcgtcctgta cgtccttcag cgcatcactg accagtgcgt
     4621 tcagcttctc cggctctttc tcaaaatcga aaaccctgcc ggaagtgtct tcctgcgcag
     4681 gttcaggctt ctgctcttgt tttatcgtca ggttatcccg ctcgctgatg gcttcccgta
     4741 gcgaggtggt cacgtcaatc acactgtcac gttcatcacg agactgctcc gcctgccttt
     4801 cagcgtccct gcgctcaagg atggcctgta actgcgcagt atcgaaccgc tgaacctgtc
     4861 ccgcgccaag atgccgctca ggctcacgct caatgccctg agctttgagt gagcgtgcat
     4921 caacgcggtc ggtatgctga taccgttcaa ggtgcttatt ctgtaggtca gcccagcgtt
     4981 cccgctgggc gacgaggtgc tctttgcgtt ctgtcggcgt ttccccgaaa cgtgcctttt
     5041 tcgcaccgcc gcgctcaggg gctttgctgt tagctcgctt aaaatactgc tgcgggtcac
     5101 ggtcgatacc gtcattcatc cgctcagaga acatgatatg cgcgtgcggc tgctcgccgc
     5161 cggctatcgc cgctttcgga ttatgaatgg cgaactgata cgcatgacgg tcgccgattt
     5221 cctgccggac aaaatcgcgg accagttcaa ggcgttgttc gggtttcagc tcacgcggca
     5281 gggcaatctc gatttcacgg taggtacagc cgttggcacg ttcaaacgtg tcagcggctt
     5341 tccagaactc cgtcggttta tgcgctgccc aggcaggcat attgccggac tccttgtgct
     5401 caaggtcgga gtctttttca cgggcatact ttccctcacg cgaaatataa tcggcatggg
     5461 gagaggcact gcctttcccg ccggttttta cgctgaggtg ataggaggcc atcgtgtttt
     5521 tatcccgctg aaggcgcgca ccgtttctga acgaagtgaa gaaacgtcta agtgcgccct
     5581 gataaataaa agagttatca gggattgtag ttgaatttga cctccactgc catcaatgag
     5641 cataattccc ccgttaaaac tcagagggta aacgggcatg agtaaaagcg aaaaacagga
     5701 acaatgggca gcagaaagag tgcagtatat tcgcggctta aagtcgccga atgagcaaca
     5761 gaaacttatg ctgatactga cggataaagc agataaaaca gcacaggata tcaaaacgct
     5821 gtccctgctg atgaaggctg aacaggcagc agagaaagcg caggaagcca gagcgaaagt
     5881 catgaacctg atacaggcag aaaagcgagc cgaagccaga gccgcccgta aagcccgtga
     5941 ccatgctctg taccagtctg ccggattgct tatcctggcg ggtctggttg acagtaagac
     6001 gggtaagcct gttgatgata ccgctgcctt actgggtgca ttagccagtc tgaatgacct
     6061 gtcacgggat aatccgaagt ggtcagactg gaaaatcaga gggcaggaac tgctgaacag
     6121 caaaaagtca gatagcacca catagcagac ccgccataaa acgccctgag aagcccgtga
     6181 cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat ccataaaagg cgcctgtagt
     6241 gccatttacc cccattcact gccagagccg tgagcgcagc gaactgaatg tcacgaaaaa
     6301 gacagcgact caggtgcctg atggtcggag acaaaaggaa tattcagcga tttgcccgag
     6361 cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc tgttttgtag aggagcaaac
     6421 agcgtttgcg acatcctttt taatactgcg gaactgacta aagtagtgag ttatacacag
     6481 ggctgggatc tattcttttt atcttttttt attctttctt tattctataa attataacca
     6541 cttgaatata aacaaaaaaa acacacaaag gtctagcgga atttacagag ggtctagcag
     6601 aatttacaag ttttccagca aaggtctagc agaatttaca gatacccaca actcaaagga
     6661 aaaggactag taattatcat tgactagccc atctcaattg gtatagtgat taaaatcacc
     6721 tagaccaatt gagatgtatg tctgaattag ttgttttcaa agcaaatgaa ctagcgatta
     6781 gtcgctatga cttaacggag catgaaacca agctaatttt atgctgtgtg gcactactca
     6841 accccacgat tgaaaaccct acaaggaaag aacggacggt atcgttcact tataaccaat
     6901 acgctcagat gatgaacatc agtagggaaa atgcttatgg tgtattagct aaagcaacca
     6961 gagagctgat gacgagaact gtggaaatca ggaatccttt ggttaaaggc tttgagattt
     7021 tccagtggac aaactatgcc aagttctcaa gcgaaaaatt agaattagtt tttagtgaag
     7081 agatattgcc ttatcttttc cagttaaaaa aattcataaa atataatctg gaacatgtta
     7141 agtcttttga aaacaaatac tctatgagga tttatgagtg gttattaaaa gaactaacac
     7201 aaaagaaaac tcacaaggca aatatagaga ttagccttga tgaatttaag ttcatgttaa
     7261 tgcttgaaaa taactaccat gagtttaaaa ggcttaacca atgggttttg aaaccaataa
     7321 gtaaagattt aaacacttac agcaatatga aattggtggt tgataagcga ggccgcccga
     7381 ctgatacgtt gattttccaa gttgaactag atagacaaat ggatctcgta accgaacttg
     7441 agaacaacca gataaaaatg aatggtgaca aaataccaac aaccattaca tcagattccc
     7501 acctacataa cggactaaga aaaacactac acgatgcttt aactgcaaaa attcagctca
     7561 ccagttttga ggcaaaattt ttgagtgaca tgcaaagtaa gtatgatctc aatggttcgt
     7621 tctcatggct cacgcaaaaa caacgaacca cactagagaa catactggct aaatacggaa
     7681 ggatctgagg ttcttatggc tcttgtatct atcagtgaag catcaagact aacaaacaaa
     7741 agtagaacaa ctgttcaccg ttacatatca aagggaaaac tgtccatatg cacagatgaa
     7801 aacggtgtaa aaaagataga tacatcagag cttttacgag tttttggtgc attcaaagct
     7861 gttcaccatg aacagatcga caatgtaaca gatgaacagc atgtaacacc taatagaaca
     7921 ggtgaaacca gtaaaacaaa gcaactagaa catgaaattg aacacctgag acaacttgtt
     7981 acagctcaac agtcacacat agacagcctg aaacaggcga tgctgcttat cgaatcaaag
     8041 ctgccgacaa cacgggagcc agtgacgcct cccgtgggga aaaaatcatg gcaattctgg
     8101 aagaaatagc gctttcagcc ggcaaaccgg ctgaagccgg atctgcgatt ctgataacaa
     8161 actagcaaca ccagaacagc ccgtttgcgg gcagcaaaac ccgtactttt ggacgttccg
     8221 gcgggttttt gtggcgagtg gtgttcgggc ggtgcgcgca agatcgctta tgttaaatac
     8281 agcagaaaaa ccataaaatt accctataac gcaaattgct ttttatgcta atatttcgca
     8341 atggagggcg aaccagtgtt taacgtcata ttgcacgatg atgtaccaaa tgagctggct
     8401 ggcttaccgc cagtagtcag agcgaagatg attcgcctga ttgataaact taaaaccaac
     8461 gctactgcat tacgagagcc agatagcaaa ccactccggg atggattatt tgagttaagg
     8521 acgatgggga cggatattgc cagaggtctt tatgtttatc agaaaggcag gaatatctat
     8581 ctacttagag tctttattaa aaagactcag aaaaccccat cgggtgaaat atctatggcc
     8641 tttgaacgtc ttaaggagat gctaaatgag taaagttaaa ggtatcgctt gggagcatgt
     8701 taggaatgaa ttgatgtcta atcctgaggt tcaagcagcc tatgaagcag aggagcgcaa
     8761 ggaaagactt caagctatgc tcgctgaatg gcgtcatcac gcaggattaa caagagccca
     8821 ggtggcagaa cgcatgggtg ttactccacc taccgtttct cgcatggaat ctaatgtgat
     8881 taaggctagc attgaaacac tggctcgata cgcaagagca tgcggtgtta agcaccctca
     8941 gatcacgttg taattttcaa ggccgcttta agcagccttt tgatgcgtgc aagatcgctt
     9001 atgttgaaca aggagttttt ttgcaaaaaa ccatcatttc acacatatga gataatccta
     9061 agggatataa ataggctaat tttgcccaac gcgccaaaaa tgtaaagtta gcatttacag
     9121 cttgtaagcc attgttctag tttcacaacg gcaaattgat gttaagtcta aaagaccgtg
     9181 ataactcatt gtggaagatt gagagctaaa tgtaacaata gaggacattg ttagtccaag
     9241 atttaagggg tgaatatgtc acccctaaga taaaacattt acagccatat ctaatacctg
     9301 caaggtgcaa aaatatcgag ttggctctga tagacggtgg tttttacatt cataagtcct
     9361 agcaaactgt cgaacaaatt gtcgtgggaa aagccgccct gttctgctcg ctgtgcaaca
     9421 caagtcatgt tcaactgatt atcttggcta aagtcattag atacccaagc cagcattggt
     9481 acgctagttt gttctttcgg tgcaatacta tagggcgcac catgtaaata catgcccttt
     9541 tcacccaaag actcaccatg gtcagagaga tacagcattg cagtatcgaa catatcctgc
     9601 ttacctttta atttattcac cacttcactg agaataaaat ccgtatatag aatagtgttg
     9661 tcgtaggtgt taatcagttc ttcttgactg caattttgaa tatcactgcg cggacaatcc
     9721 ggtataaatt tacgatgctc tggcgggtat ctaagataat aagttggtcc atgactacca
     9781 atgatatgca aaaaaattac tgtatcttga cttggtgcta ctgccagaat tttatctaat
     9841 ttgttgagca atacttggtc aaaacaatat tggccagaac acagcttcgg atcactcttc
     9901 aaatctatcg tgagattttc aacctgatca cacacacctt tacagccaga atcattatca
     9961 aaccactgta cttttatacc actatgactt aacacatcaa tcactgtgtc ttgagcatta
    10021 gcacggcgag gatcatagtc tgcccgcccc attcgtgaaa acatacaggg tagagacacc
    10081 gccgtggccg tgccgcatga gctagtatcg ttaaacgcaa tcagcccctg attttgggta
    10141 tgagcattgg ttggcttgtt atatccataa tattgatagc tcattgagcg cgcagtttca
    10201 cccacaacaa acactaataa gttaggttta gtgttcgggt tacgactggc attcttcgca
    10261 tctaggccaa gttgttggta ttccatgggc gtctgcaaat agtgctcatt gagatattta
    10321 gatgcactac tgacaaaata ggtagggaca atgtaacgcc ttaactcact gttgtttcga
    10381 ccaaatgcag cataatcttg atagtaaaaa aaggcgacta tcccaatgcc aacgaacatt
    10441 agcagcataa aggctaattt atgcaataac tccttaaaaa agggctgata atgaatatcg
    10501 gccttataaa taagatatga cggtaatagc ccagtcagca gtagattggt aattgatgca
    10561 agatttacat acatcaatgc ttcagcagga tgtgtttgaa acgtgttttc tatcatgccg
    10621 tagtcaaaca cgacattgta ttgataggct gcaaaaaata cacttgagga aagtaacgtc
    10681 aatacgataa aaaagggctt cagcaggtat ttgacggtaa aaattgaaaa caaaaaactc
    10741 agcgcaaatg ttaaaaatag cggcatagat gcgatgaaaa ggggatcaac ttctggttgt
    10801 ttttcaattc cttttcgcac tataccaaag agcggtagat tgaaaatggc aacataaaac
    10861 aacgcagtga tgaaagtgaa ttggttaacc gataacgtct taaatctaga aatcacaaaa
    10921 accccttttt tgctaaaaaa aacaatacta gcttgacctc attaaaataa ccttatgatt
    10981 aaaaggttat tttaatgagt caccaacaaa cttaacagat aagaccgttt taggtgaatt
    11041 gagcccaatt cacctaaaag ttacatgtaa acaattaagt aaataaactt caattactta
    11101 taagtttact tgtaatttct tgtgtgctct tcacagaatt cagtgcccga taaatagagg
    11161 cttctccaag gtgatactcc ttggcaagct gcccaatggt ggccccctca gctcttttat
    11221 cataaatagc ctgttttaag gtatccgtga gttttacagg cactgttgca aatagtcggt
    11281 ggtgataaac ttatcatccc cttttgctga tggagctgca catgaaccca ttcaaaggcc
    11341 ggcattttca gcgtgacatc attctgtggg ccgtacgctg gtactgcaaa tacggcatca
    11401 gttaccgtga gctgcaggag atgctggctg aacgcggagt gaatgtcgat cactccacga
    11461 tttaccgctg ggttcagcgt tatgcgcctg aaatggaaaa acggctgcgc tggtactggc
    11521 gtaacccttc cgatctttgc ccgtggcaca tggatgaaac ctacgtgaag gtcaatggcc
    11581 gctgggcgta tctgtaccgg gccgtcgaca gccggggccg cactgtcgat ttttatctct
    11641 cctcccgtcg taacagcaaa gctgcatacc ggtttctggg taaaatcctc aacaacgtga
    11701 agaagtggca gatcccgcga ttcatcaaca cggataaagc gcccgcctat ggtcgcgcgc
    11761 ttgctctgct caaacgcgaa ggccggtgcc cgtctgacgt tgaacaccga cagattaagt
    11821 accggaacaa cgtgattgaa tgcgatcatg gcaaactgaa acggataatc ggcgccacgc
    11881 tgggatttaa atccatgaag acggcttacg ccaccatcaa aggtattgag gtgatgcgtg
    11941 cactacgcaa aggccaggcc tcagcatttt attatggtga tcccctgggc gaaatgcgcc
    12001 tggtaagcag agtttttgaa atgtaaggcc tttgaataag acaaaaggct gcctcatcgc
    12061 taactttgca acagtgccgc ccctctggct aacggaaatt tcggcggttc cggcttacaa
    12121 cccctataag tcagcggaca agttctttgt tatatagcca ccagccacca cacgcgaaat
    12181 gtgttttgct ggggcagctg ggttggcact acctgtttcc ggcatcccat ctgagtcgcg
    12241 atcctgtcga tggggccttg cgtcgacatc atctggatcc aaccaccctg caaaaagcgg
    12301 ttcgtgccac ggcgcgtcag ttgcagctgg gaaaaccggt cacctgccat accttgcgcc
    12361 attcctttgc cacccatttg ctggcgcggg gggcggatat tcgaaccgtg caggagcaac
    12421 tgggccattc tgatgttcga accacacaaa tttataccca tgtgctgcaa aacggcgcca
    12481 atggggtacg tagccctttg tctgatctgt aatggttggg gggcgggacg ctcggttttt
    12541 cagaacaagt ggcctgacgc aggcacgcta ccgcccttag gttacagctc ctagaggctg
    12601 acatccgttt caggtggtgg cttgttaccc gggccatttt catcctgatg gcctgttctt
    12661 ttcgtcaaac tatgctacgg atttcagttt catatggaaa taaataatcg acgggatttg
    12721 caaaaatgtg cgatagacag ggaaggtgcg aacaagttcc tgatatgaga tcatcatgtt
    12781 catccggagc gcatcccaga gggacatc
//
