LOCUS       NZ_CP119752             6668 bp    DNA     circular CON 19-MAY-2024
DEFINITION  Escherichia coli strain E15 plasmid pE15-6, complete sequence.
ACCESSION   NZ_CP119752
VERSION     NZ_CP119752.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33693438
            Assembly: GCF_029319085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   zhu,Q., zhu,z., wang,C.Y., wu,L.Y., wang,W. and zhang,J.Y.
  TITLE     Genetic features of mcr-1-producing Escherichia coli isolated in
            Chinese bovine and chicken
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   zhu,Q., zhu,z., wang,C.Y., wu,L.Y., wang,W. and zhang,J.Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2023) Lanzhou Institute of Animal Husbandry and
            Veterinary Medicine, Chinese Academy of Agricultural Sciences, No.
            335 along the alkali ditch in Qilihe District, lanzhou, lanzhou
            730050, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2022
            Assembly Method                   :: SPAdes v. 3.15.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 227.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029319085.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 05:44:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,374
            CDSs (total)                      :: 5,254
            Genes (coding)                    :: 4,987
            CDSs (with protein)               :: 4,987
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 267
            CDSs (without protein)            :: 267
            Pseudo Genes (ambiguous residues) :: 0 of 267
            Pseudo Genes (frameshifted)       :: 104 of 267
            Pseudo Genes (incomplete)         :: 174 of 267
            Pseudo Genes (internal stop)      :: 49 of 267
            Pseudo Genes (multiple problems)  :: 55 of 267
            Pseudo Genes (short protein)      :: 1 of 267
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119752.1.
            Bacteria and source DNA available from chicken.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E15"
                     /host="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pE15-6"
                     /geo_loc_name="China:Hebei"
                     /collection_date="2020-08"
     gene            join(6530..6668,1..281)
                     /locus_tag="P1J19_RS26830"
                     /old_locus_tag="P1J19_26830"
     CDS             join(6530..6668,1..281)
                     /locus_tag="P1J19_RS26830"
                     /old_locus_tag="P1J19_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(599..853)
                     /locus_tag="P1J19_RS26835"
                     /old_locus_tag="P1J19_26835"
     CDS             complement(599..853)
                     /locus_tag="P1J19_RS26835"
                     /old_locus_tag="P1J19_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015059032.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(860..1378)
                     /locus_tag="P1J19_RS26840"
                     /old_locus_tag="P1J19_26840"
     CDS             complement(860..1378)
                     /locus_tag="P1J19_RS26840"
                     /old_locus_tag="P1J19_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1416..2114)
                     /locus_tag="P1J19_RS26950"
                     /pseudo=""
     CDS             complement(<1416..2114)
                     /locus_tag="P1J19_RS26950"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2056..2379)
                     /locus_tag="P1J19_RS26850"
                     /old_locus_tag="P1J19_26850"
     CDS             complement(2056..2379)
                     /locus_tag="P1J19_RS26850"
                     /old_locus_tag="P1J19_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2443..2634
                     /locus_tag="P1J19_RS26855"
                     /old_locus_tag="P1J19_26855"
     CDS             2443..2634
                     /locus_tag="P1J19_RS26855"
                     /old_locus_tag="P1J19_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3304..3462
                     /locus_tag="P1J19_RS26955"
     CDS             3304..3462
                     /locus_tag="P1J19_RS26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            3960..4187
                     /locus_tag="P1J19_RS26860"
                     /old_locus_tag="P1J19_26860"
     CDS             3960..4187
                     /locus_tag="P1J19_RS26860"
                     /old_locus_tag="P1J19_26860"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(4184..5752)
                     /gene="cea"
                     /locus_tag="P1J19_RS26865"
                     /old_locus_tag="P1J19_26865"
     CDS             complement(4184..5752)
                     /gene="cea"
                     /locus_tag="P1J19_RS26865"
                     /old_locus_tag="P1J19_26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            6012..6449
                     /locus_tag="P1J19_RS26870"
                     /old_locus_tag="P1J19_26870"
     CDS             6012..6449
                     /locus_tag="P1J19_RS26870"
                     /old_locus_tag="P1J19_26870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     Typing          complement(2720..2807)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          2818..3376
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(496..2066)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acttttccac ccaaaaagcc gacacaacac gaatgatggt gccttttttt cggcagttct
       61 gggatatggg agctaaagat aaagcgacag gaaaatcacg gagtgatgtg caacaacgca
      121 ttcagcagtt tcacagccag gaatttttaa actcactccg gggcacaact caatttgcgg
      181 gtactgatta ccgcagcaaa gaccttaccc cgaaaaaatc caggctgctg gctgacacga
      241 tttctgcggt ttatctcgat ggctacgagg gcagacagta agtggattta ccataatccc
      301 ttaattgtac gcaccgctaa aacgcgttca gcgcgatcac ggcagcagac aggtaaaaat
      361 ggcaacaaac cacccgaaaa actgccgcga tcgcgcctga taaattttaa ccgtatgaat
      421 acctatgcaa ccagagggta caggccacat tacccccact taatccactg aagctgccat
      481 ttttcatggt ttcaccatcc cagcgaaggg ccatccagcg tgcgttcctg tatttccggc
      541 tgacgctccc gttccaggga taacacatgt tcgcgctcct gtatcagccg ttcctctctt
      601 atctccagtt ctcgctgtat aactggctca agcgttctgt ctgctcgctc aagcgttctg
      661 tctgctcgct caagtgctgc acctgctgac tcaactgcat gacccgctcg ttcagcatcg
      721 cgttgtcccg ttgcgtaagc gaaaacatct tctgcaattc cacgaaggcg ctctcccatt
      781 cgctcagccg ctgcatatag tcctgttgca gctgctctaa ggcgttcagc aaatgtgttt
      841 ccagctctgt cactctgtgt cactccttca gatgtaccca ctctttcccc tgaaagggaa
      901 tcacctccgc tgatttcccg tacggaagga caaggaattt cctgttcccg tcctgcacaa
      961 actccacgcc ccatgtcttc gcgttcagtt tctgcaatgt ctcttcctgc ttcctgattt
     1021 cttccaggtt cgcctgtatc ctccctccaa gataccagag cgtcccgcca ctcgcggtaa
     1081 acaggagaaa gactatcccc agtaacatca tgcccgtatt ccctgccagc tttaacacgt
     1141 ccctcctgtg ctgcatcatc gcctctttca ccccttcccg gtgtttttcc agcgattcct
     1201 ctgtcgaggc tgtgaacagg gctatagcgt ctctgatttt cgtctcgttt gatgtcacag
     1261 cctcgcttac agattcgccg agcctcctga actcgttgtt cagcattttc tctgtagatt
     1321 cggctctctc tttcagcttt ttctcgaact ccgcgcccgt ctgcaaaaga ttgctcataa
     1381 aatgctcctt tcagcctgat attcttcccg ccgttcggat ctgcaatgct gatactgctt
     1441 cgcgtcaccc tgaccacttc cagccccgcc tcagtgagcg cctgaatcac atcctgacgg
     1501 ccttttatct ctccggcatg gtaaagtgca tctatacctc gcgtgacgcc ctcagcaagc
     1561 gcctgtttcg tttcaggcag gttatcaggg agtgtcaacg tcctgcggtt ctccggggcg
     1621 ttcgggtcat gcagcccgta atggtgattc accagcgtct gccaggcatc aattctaggc
     1681 ctgtctgcgc ggtcgtagta cggctggagg cgttttccgg tctgtagctc catgttcgga
     1741 atgacaaaat tcagctcaag ccgtcccttg tcctggtgct ccacccacag gatgctgtac
     1801 tgattttttt cgagaccggg catcagtaca cgctcaaagc tcgccatcac tttttcacgt
     1861 cctcccggcg gcagctcctt ctccgcgaac gacagaacac cggacgtgta tttcttcgca
     1921 aatggcgtgg catcgatgag ttcccggact tcttccggat taccctgaag caccgttgcg
     1981 ccttcgcggt tacgctccct ccccagcagg taatcaaccg gaccactgcc accacctttt
     2041 cccctggcat gaaatttaac tatcatcccg cgccccctgt tccctgacag ccagacgcag
     2101 ccggcgcagc tcatccccga tggccatcag tgcggccacc acctgaaccc ggtcaccgga
     2161 agaccactgc ccgctgttca ccttacgggc tgtctgattc aggttatttc cgatggcggc
     2221 cagctgacgc agtaacggcg gtgccagtgt cggcagtttt ccggaacggg caaccggctc
     2281 ccccaggcag acccgccgca tccataccgc cagttgttta ccctcacagc gttcaagtaa
     2341 ccgggcatgt tcatcatcag taacccgtat tgtgagcatc ctctcgcgtt tcatcggtat
     2401 cattacccca tgaacagaaa tcccccttac acggaggcat cagtgactaa acaggaaaaa
     2461 accgccctta acatggcccg ctttatcaga agccagacat taacgctgct ggagaagctc
     2521 aacgaactgg acgcagatga acaggccgat atttgtgaat cgcttcacga ccacgccgat
     2581 gagctttacc gcagctgcct cgcacgtttc ggggatgacg gtgaaaacct ctgacacatg
     2641 cagctcccgg agacggtcac agcttgtctg tgagcggatg ccgggagctg acaagcccgt
     2701 cagggcgcgt cagcaggttt tagcgggtgt cggggcgcag ccctgaccca gtcacgtagc
     2761 gatagcggag tgtatactag cttaaccatg cggcatcagt gcggattgta tgaaaagtac
     2821 gccatgccgg gtgtgaaatg ccgcacagat gcgtaaggag aaaatgcacg tccaggcgct
     2881 tttccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggtac
     2941 tgactcacac aaaaacggta acacagttat ccacagaatc aggggataag gccggaaaga
     3001 acatgtgagc aaaagaccag gaacaggaag aaggccacgt agcaggcgtt tttccatagg
     3061 ctccgccccc ctgacgagca tcacaaaaat agacgctcaa gtcagaggtg gcgaaacccg
     3121 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3181 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3241 tctcatagct cacgctgttg gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3301 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3361 gagtccaacc cggtaaggca cgccttaacg ccactggcag cagccactgg taaccggatt
     3421 agcagagcga tgatggcaca aacggtgcta cagagttctt gaagtagtgg cccgactacg
     3481 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3541 aaagagttgg tagctcttga tccggcaaac aaaccaccgt tggtagcggt ggtttttttg
     3601 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3661 ctactgaacc gcgatccccg tcagtttaga agaggaggat ggtgcgatgg tccctccctg
     3721 aacatcacgt atatagttag cctgacatcc aacaaggagg tttatcgcga atattcccac
     3781 aaaaaatctt ttcctcataa ctcgatcctt ataaaatgaa aagaatatat ggcgaggttt
     3841 aatttatgag cttaagatac tacataaaaa atattttatt tggcctgtac tgcacactta
     3901 tatatatata ccttataaca aaaaacagcg aagggtatta tttccttgtg tcagataaga
     3961 tgctatatgc aatagtgata agcactattc tatgtccata ttcaaaatat gctattgaat
     4021 acatagcttt taacttcata aagaaagatt ttttcgaaag aagaaaaaac ctaaataacg
     4081 cccccgtagc aaaattaaac ctatttatgc tatataatct actttgtttg gtcctagcaa
     4141 tcccatttgg attgctagga ctttttatat caataaagaa taattaaatc cctaacacct
     4201 catttatagt attaagttta ttcttatcaa tataggagca tagaattcct gtaacaatag
     4261 caatacccca aatacctaat gtagttccag caagcaagct aaaaagtaaa gcaacaacat
     4321 aactcacccc tgcatctgca gctttcttct ctaatgtaag aaatagtggc ttccagtcac
     4381 ctgtatcctt aatttttagg atatcagata ccacatcata tccaaaagaa acatgccccg
     4441 taatcttcaa gtacttggca aactgatcta aatgtttagc ccagtcatca tacttcaccg
     4501 atgccaacgc attaaaaata gcatcacgat cggctttgct gaatttctta tttaaaacat
     4561 ccttgtattt ttcaaaagca gcgagagctt cattcacatt gccgattttc ttacctttag
     4621 acttatcagc aagttcctgt gccattttcg aatatttttc accatatttt tcagtcagcg
     4681 tttgataaaa gctaactgtt gcatcaacag catccttaat ctgtgaatta aggagattat
     4741 tctgtgcttt tttcaaattt tcttcagctt catgaacacg agcgataccg gcattacgat
     4801 tattactgac ctgagaaata gccttctgga tctgagttat atcagcattt atccggttaa
     4861 tacgtgtttc tgatgctgtt acctgttttt gtttttcttc tctaatctta ccggccccaa
     4921 cccgtcgtct ggttgcttca aaaaaaggac ggttctgaag cggatcattg gctcttggtg
     4981 atagtttttt gaccagctca tccagttctt tatatttagc ggatgcctga gccagttcat
     5041 ttcgttttcc agcgagcgtt ttcatttctg catcacgggc atggatactg gagcttaaac
     5101 gagaattgag agtcttaatc tctccatcca ttttcaccac ttcagattgt gcagcagaaa
     5161 gttttttttg ggcgatctca acagctttag cttcttcact caatgcagcc agtcgtttct
     5221 cttcagcttc agccagtttc aactggcgtt ctgtttcagc cttctcccgt tcaatctctt
     5281 tacgtcgttg ttctgcttcc tgaaaagcct tttctgctgc ttccgcttct ttacgggctt
     5341 tttcttctgc tttcgcaagg cgcaaacgct ctgcttccgc ctgcatagct gcattattag
     5401 catgagcaag ctctgttgct gaaggcgtac gtgaggcatt gtgacgaaga gcctcattca
     5461 cgatatcctt caggcgctga gtcagcgcat ccctgtttgc ctttgctttc gcctgtgctt
     5521 ccgctgcagc ttttgcccgg gcagcctgct ctgcctgtgt tttctttaat tgagcagtag
     5581 accatttagc agttgcatga atagctgcag aactttcact tttactgcct ccttttccac
     5641 ctccgccgcc agagccactc ccgtcaggag taccattcaa aagagtaata attacctgtc
     5701 ccttatcatc ataaggaaca ccatctttat agtacgctac cgcggtttcc attataaaat
     5761 cctctttgac ttttaaaaca ataagttaaa aataaatact gtacatataa ccactggttt
     5821 tatatacagc ataaaagcta cgccgctgca ttttccctgt caagactgtg gacttccatt
     5881 tttgtgaaaa cgatcaaaaa aacagtcttt cacaccacgt gctattctcg cccgatgcca
     5941 caaaaaccag cacaaacatt accgttctca gacctcatta tgttttactg aaactatgag
     6001 atgagacatc tatgggacac tgtcacttta tggcatggca cacactccgg gacgcactaa
     6061 aaatgacagg cagatcgcgt tcacagtttt accgtgatat gcgcgcaggc cttgtcagtt
     6121 accgtaccgg cagggacgga cgacgggagt ttgaaaccag tgaactgatc cgggcatacg
     6181 gcgaattaaa gcagaatgag acaccagaaa ggcacagtga gggacatgca gaaaatccac
     6241 atgatcagca gacagaacgc attctccggg agctgaatga gctgaaacaa tgcctgacgc
     6301 tgatgcttga ggataaacag gcacaggata tggatcgcag acgccaggaa gcagaacggg
     6361 aacagctaca aaatgagata gcccagctca ggcaggcact ggaactggaa aagaaacggg
     6421 gattctggtc caggttgttc ggtcgctgaa cgctgtcaga gactgatgat aaaatagtct
     6481 tcggataata actcaccgag aataaatact ttaaggtagg gagacactca tgagacgtac
     6541 cggaaacaaa ctttgtctta tcgccatgat aacagcaaca gtagctctca cagcctgtac
     6601 cccaaagggc agcgtggaac aacatacccg gcattacgta tatgcttctg atgacggttt
     6661 tgatccca
//
