LOCUS       NZ_CP119751             6747 bp    DNA     circular CON 19-MAY-2024
DEFINITION  Escherichia coli strain E15 plasmid pE15-5, complete sequence.
ACCESSION   NZ_CP119751
VERSION     NZ_CP119751.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33693438
            Assembly: GCF_029319085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   zhu,Q., zhu,z., wang,C.Y., wu,L.Y., wang,W. and zhang,J.Y.
  TITLE     Genetic features of mcr-1-producing Escherichia coli isolated in
            Chinese bovine and chicken
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   zhu,Q., zhu,z., wang,C.Y., wu,L.Y., wang,W. and zhang,J.Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2023) Lanzhou Institute of Animal Husbandry and
            Veterinary Medicine, Chinese Academy of Agricultural Sciences, No.
            335 along the alkali ditch in Qilihe District, lanzhou, lanzhou
            730050, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2022
            Assembly Method                   :: SPAdes v. 3.15.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 227.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029319085.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 05:44:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,374
            CDSs (total)                      :: 5,254
            Genes (coding)                    :: 4,987
            CDSs (with protein)               :: 4,987
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 267
            CDSs (without protein)            :: 267
            Pseudo Genes (ambiguous residues) :: 0 of 267
            Pseudo Genes (frameshifted)       :: 104 of 267
            Pseudo Genes (incomplete)         :: 174 of 267
            Pseudo Genes (internal stop)      :: 49 of 267
            Pseudo Genes (multiple problems)  :: 55 of 267
            Pseudo Genes (short protein)      :: 1 of 267
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119751.1.
            Bacteria and source DNA available from chicken.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E15"
                     /host="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pE15-5"
                     /geo_loc_name="China:Hebei"
                     /collection_date="2020-08"
     gene            279..1205
                     /locus_tag="P1J19_RS26800"
                     /old_locus_tag="P1J19_26800"
     CDS             279..1205
                     /locus_tag="P1J19_RS26800"
                     /old_locus_tag="P1J19_26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_024129980.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLGELADYLDLSASA
                     RRSIDKRYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRGAVATS
                     ARTLKPWETLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1411..1686)
                     /locus_tag="P1J19_RS26805"
                     /old_locus_tag="P1J19_26805"
     CDS             complement(1411..1686)
                     /locus_tag="P1J19_RS26805"
                     /old_locus_tag="P1J19_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039023505.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADMNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            1865..4084
                     /gene="mobQ"
                     /locus_tag="P1J19_RS26810"
                     /old_locus_tag="P1J19_26810"
     CDS             1865..4084
                     /gene="mobQ"
                     /locus_tag="P1J19_RS26810"
                     /old_locus_tag="P1J19_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_063114182.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKSRPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLEKQIESLLRTYRAVKERDIPARRQAFETQWNTWIS
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEYDARLKHHDNHRLSRETALAGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIRER
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPAA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCEGPEEMLVGFVRLEDELRPV
                     LALPSPDYSHLMVSIITENGIHLAGYGEAINRDADTPYPPEPKLMQFRLKGCQDTLFAA
                     INKPEEMPDYLFRQLGFNQTWHEWKREEQRRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            4316..6064
                     /gene="col"
                     /locus_tag="P1J19_RS26815"
                     /old_locus_tag="P1J19_26815"
     CDS             4316..6064
                     /gene="col"
                     /locus_tag="P1J19_RS26815"
                     /old_locus_tag="P1J19_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E9"
                     /protein_id="WP_024129977.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGIGVSGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGRGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISASELSAAIAGIIAKLKKVNLKFTPFGVVLSSLIPSEIAKDDPNMMSKIVTSLPAD
                     DITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFT
                     ASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGH
                     NAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVAR
                     NQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQ
                     RAQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGK
                     ATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSK
                     QFNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHI
                     DIHRGQ"
                     /name="col"
     gene            6067..6324
                     /locus_tag="P1J19_RS26820"
                     /old_locus_tag="P1J19_26820"
     CDS             6067..6324
                     /locus_tag="P1J19_RS26820"
                     /old_locus_tag="P1J19_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000421100.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            6386..6529
                     /locus_tag="P1J19_RS26825"
                     /old_locus_tag="P1J19_26825"
     CDS             6386..6529
                     /locus_tag="P1J19_RS26825"
                     /old_locus_tag="P1J19_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..6747
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1691..1864)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(1865..2223)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          280..1205
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.935"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1866..4081
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.693"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaacgattc tgacgcattt ttctggttca gcctggtgtt tccttgtctt tttgcgtttt
       61 ttgcgtcaga acgcgtctga gagcgtttta aggggtacgt acaacaggaa ttatggtaaa
      121 tggatcgggt ttgcgggaag gatcgacagg atttgccgtt gggtgtagtg taagcgactg
      181 aaaaacaaac gcccctgaaa tcatgtccag atccggcaag attcaacatg aaatacagag
      241 ggcgtgtagg tcgcataacc cggagagaat cgtaacacat gagcgccgcg cttcaatact
      301 tcgacgaaaa tttaccccac cgtccctatc acacggatga tctcgctttt ggtcttcgca
      361 tctccggcaa agggcgtgcg cttcttgcgc ggtacatcca gcagaaccag cctcatgcgc
      421 agttctggct ggtttttgac gttgaccgcg agggggcagc gattgactgg agtgaccgga
      481 acgctcccgc gccgaacatc accgtaaaaa atcctgtgaa cggtcatgct cacctgctct
      541 acgcgctcaa tatcgccgtg agaaccgcgc cggacagctc ggttaaggcg ctgaaatacg
      601 ccgctgcgat tgaacgttcg ctgtgtgaga aactttgtgc tgatgtgaac tacagcggac
      661 tgatttgtaa aaatccgttc cacctggaat ggcaggtgat ggagtggcga gaggaagcct
      721 ataccctcgg tgaactggct gattatctcg atttgagtgc ctcagcgcgc cgtagcatcg
      781 ataaacgtta cgggatgggg cgaaactgcc acctgttcga aatgacgcgc aaatgggctt
      841 acagggcgat tcgtcagggc tggccagcat tctcacagtg gcttgatgcc gtgattcagc
      901 gtgtcgaaat gtacaacgca tcgcttcccg ttccgctttc tccggctgaa tgtcgggcta
      961 ttggcaagag cattgcgaaa tacacgcaca ggaacttcac gccggaaact ttcgcacagt
     1021 atgtggctga tacgcacacg ccagaaattc aggctgcacg tggtcgcaag ggcgggaaaa
     1081 ttggaggtgc taagtctaag cgtggtgctg ttgccacatc tgcgcgaacg ttgaaaccgt
     1141 gggagactct tggaattagc cgcgcctggt attaccaact gaaaaaacga ggtcttgtag
     1201 agtagaccaa ataaaaccta tatcagataa cagcgctttt ttgagctgtt ggcgtttggt
     1261 ggctttttct tttcctccct gcaatggtgc ggctttccgc gtgattgagg ttgtagcgct
     1321 cgccgcagtc tcatgtccga gcgtagcgag cgaatgagcg aggaagcgca aaggcgtccg
     1381 gtggtgtatg tggctcttac gcgccggggc ttagtggttc tgcggtttcg ccggtggtct
     1441 gggtagcttc tccagctcgt taatcagcgg ttgtagtcgg ttcatatcgg cctgtcttgt
     1501 gacttccttt cgcagaaact ggagcaggaa cgcacgcagt tgcgcttctt ccggcctccg
     1561 taccctcgcc agcatggcag cccccacaat gactttttgc gccgtgtcca ggctccggct
     1621 cttcgccttc aggcgctgta atctggcctc agcttcggca atcttctgtt cgagtgttct
     1681 gctcatttcg tgactccgtg cgcggtgaaa aatcgtattt tagcgcgtca ctggtagttg
     1741 caaaactgaa ctggcataat gcacggcaca tcacgaagtg cgcacttata caatcttcac
     1801 ttcgtttcga ttgtgtgcgc cctgcggggc tgaaagaaaa cggcaaaaag gcattacgga
     1861 agaaatggcg atttatcatc tcagcatgaa aatcatttcg cgaaaaaacg gctacagtgc
     1921 cgttgcttct gctgcctacc gttccggctc tgtcataccc gatgaccgta ccggattaat
     1981 ccacgattac acccgtaaac gcggcgttga tgatgcggtc attctcaccc ctgcgaatgc
     2041 accgtcctgg tgtggtgacc gttccgttct ctggaatgcg gtcgagaaag ccgaacagcg
     2101 ccggaactcc cagctggcaa gggagattga actcgccatt ccccgtgaga tttcccgcga
     2161 ggccgcacgg gaggccgttc tcgcttttgt ccgggaaaac tttgtcagtc ggggcatgat
     2221 tgccgatgtg gcgttccatc atatggaccg gaccaacccc catgcacaca tcatgctgac
     2281 cacgagagct gtcggggaaa cgggattcgc aggaaaggtc agggactgga acgaccgggc
     2341 actcgccgag acgtggcgcg catcatgggc tgaccatgcg aacagagcgc ttgcgaacgc
     2401 cggctaccag gaagagatag accatcgttc atacgagcgt cagggactgg agaaagcgcc
     2461 gggccttcac ctcggaaagg ctgcctgtgc gatggaaaaa cggggcatgg aaacagaacg
     2521 cggtgagcag aaccgcctga ttaacagcct taacctggaa atacaggttt cccgcacaag
     2581 gctggcagtc atccaggtac aggagaacgt gcggaaacgg gaactcagcg atgccgcacg
     2641 tcgtgcagcg gaagccctta atctgaccat tcccgccgcg aatgcctcag cggataccct
     2701 gcgggaattc attgccacac tgccgcagga atgcgggaac gcatgggaga tgacaccgga
     2761 gttcgtggcg atgaacggga aggcaaacga catcaaacgt gaggggaatg cgctggtgaa
     2821 agagcgggat attctcgaaa aagagatgac cggactcaga aaatcccgtc cggtagcgtc
     2881 catcctgtca gagcttcccc tgatgacatg ggctgaaccg gaataccgca aaagacaact
     2941 ccgcttctgg aaactcgaga aacagattga atctcttctc cgcacctaca gggccgtgaa
     3001 agaacgggac attcccgccc gtcgtcaggc ctttgaaacg cagtggaata cgtggatttc
     3061 gccgggaatg gcagagctga aagaaaaact gtcagcacgg gaagcggagc ggcgcaggga
     3121 ggaagccgaa gcggaagcgc gccggaagga acaggaatat gacgcacggc tgaaacacca
     3181 tgataaccat cgcctgagcc gtgaaacggc attagccggg gttattacgg agctggggcg
     3241 tgccagagag ccgggaacgg gcaggataac ccgctacatg atgctgagta acagagccgg
     3301 agaattcacg gtatggggtg atgagctggc gcattacccc cagagtgttc atgacccggt
     3361 gaatgtttac ctgtcgccag gcggggctgt gatggtctcg gatatacgtg agagaatgcc
     3421 agaatctcat gagacgatgg cgcggcctga gcgtgtgaga atgtattccg gtgcgacggt
     3481 ccggcatgta ctggaacaga tgcgccaggg gtggccctct tacggttttc cggcgctgcc
     3541 gcatcactgg ccggataatt tttatttcag cgacgaccgc aggcccgcag cctctccgct
     3601 gccgtcagcg caccgggtgg acgtcaccgc ttatgcggca ccggagcaac tcatgcccgt
     3661 tgtcttttcg acagagcgaa acagcaggac gctgaatctg ctgttgtgcg aagggccgga
     3721 ggaaatgctt gtcggttttg tgcgcctgga ggacgagctg cgtcccgttc ttgcgcttcc
     3781 gtcgccggat tacagtcatc tgatggtaag catcatcaca gagaacggga tacacctggc
     3841 aggttacggt gaggccataa accgtgatgc tgatactccg tacccaccgg aaccgaagct
     3901 gatgcagttc aggcttaagg gctgtcagga cacgcttttt gctgccatca acaaaccgga
     3961 agagatgccg gattatctct tccgtcaact cggatttaat cagacctggc atgagtggaa
     4021 gcgggaggaa cagcgcaggc aacaacaacg ccgtcccggg cacttccggg gcatgagtat
     4081 gtgatgtccg gggctgcact ccggaccccg ccaacacatc acgggccaca aaattttttg
     4141 tggcccgctc tgcgttttct aagtgttatc cctcctgact tctaaaaaat tttccacctg
     4201 aacttgacag aaaaaacgat gacgggtact ttttgatctg tacataaaac cagtggtttt
     4261 atgtacagta ttaatcatgt aattaattgt tttaacgctt aaaagaggga cttttatgag
     4321 cggtggagat ggacgcggcc ataacacggg cgcgcatagc acaagtggta acattaatgg
     4381 tggcccgacc gggattggtg taagtggtgg tgcttctgat ggttcaggat ggagttcgga
     4441 aaataacccg tggggtggtg gttccggtag cggcattcac tggggaggtg gctccggtcg
     4501 tggtaatggc gggggtaatg gcaattccgg tggtggctcg ggaacaggcg gtaatttgtc
     4561 agcagtagct gcgccagtgg catttggttt tccggcgctt tccactccag gagctggcgg
     4621 tctggctgtc agtatttctg caagcgaatt atcggcagct attgctggta ttattgctaa
     4681 attaaaaaaa gtaaatctta aattcactcc ttttggggtt gtcttatctt cattaattcc
     4741 gtcggaaata gcgaaagatg accccaatat gatgtcaaag attgtgacgt cattacccgc
     4801 agatgatatt actgaatcac ctgtcagttc attacctctc gataaggcaa cagtaaacgt
     4861 aaatgttcgt gttgttgatg atgtaaaaga cgaacgacag aatatttcgg ttgtttcagg
     4921 tgttccgatg agtgttccgg tggttgatgc aaaacctacc gaacgtccag gtgtttttac
     4981 ggcatcaatt ccaggtgcac ctgttctgaa tatttcagtt aataacagta cgccagcagt
     5041 acagacatta agcccaggtg ttacaaataa tactgataag gatgttcgcc cggcaggatt
     5101 tactcagggt ggtaatacca gggatgcagt tattcgattc ccgaaggaca gcggtcataa
     5161 tgccgtatat gtttcagtga gtgatgttct tagtcctgac caggtaaaac aacgtcagga
     5221 tgaagaaaat cgccgtcagc aggaatggga tgctacgcat ccggttgaag cggctgagcg
     5281 aaattatgaa cgcgcgcgtg cagagctgaa tcaggcaaat gaagatgttg ccagaaatca
     5341 ggagcgacag gctaaagctg ttcaggttta taattcgcgt aaaagcgaac ttgatgcagc
     5401 gaataaaact cttgctgatg caatagctga aataaaacaa tttaatcgat ttgcccatga
     5461 cccaatggct ggcggtcaca gaatgtggca aatggccggg cttaaagctc agcgggcgca
     5521 gacggatgta aataataagc aggctgcatt tgatgctgct gcaaaagaga agtcagatgc
     5581 tgatgctgca ttaagtgccg cgcaggagcg ccgcaaacag aaggaaaata aagaaaagga
     5641 cgctaaggat aaattagata aggagagtaa acggaataag ccagggaagg cgacaggtaa
     5701 aggtaaacca gttggtgata aatggctgga tgatgcaggt aaagattcag gagcgccaat
     5761 tccagatcgc attgctgata agttgcgtga taaagaattt aaaaacttcg acgattttcg
     5821 gagaaaattc tgggaagaag tgtcgaaaga tcctgagtta agtaagcaat ttaatccagg
     5881 taataaaaaa cgcttgagcc aaggattagc tccacgggct aggaataaag atactgtcgg
     5941 tgggcgtcgc tcatttgagc ttcatcacga caagccaatc agtcaggatg gtggtgttta
     6001 tgatatggac aacctccgta tcactacgcc gaaacgccat attgatattc acagaggtca
     6061 gtaaaaatgg aactgaaaaa cagcattagt gattacactg aaactgaatt caaaaaaatt
     6121 attgaagaca tcatcaattg tgaaggtgat gaaaaaaaac aggatgataa cctcgagcat
     6181 tttataagtg ttactgagca tcctagtggt tctgatctga tttattaccc agaaggtaat
     6241 aatgatggta gccctgaagc tgttattaaa gagattaaag aatggcgagc tgctaacggt
     6301 aagtcaggat ttaaacaggg ctgaaatatg aatgccagtt gtttaaggat gaatgactgg
     6361 cattctttca caacaaggag tcgttatgaa aaaaataaca gggattattt tattgcttct
     6421 tgcagtcatt attctggctg catgtcaggc aaactatatc cgggatgttc agggcgggac
     6481 cgtatctccg tcatcaacag ctgaagtgac cggattagca acgcagtaac ccgaaatcct
     6541 ctttgacaaa aacaaagcgt gtcaggctga ttctgatgcg cttttttttg aaatgtcaca
     6601 aaagttccat gtgggagatg ggatctaaaa tccctgtgca gaaccttcca tccggtggga
     6661 gaaaacttgt cattttgacc tgttcgccat tcggaacggt cgaaaccgat cgcgtatcgc
     6721 tttcgtgcat agttatgcag cgcccta
//
