LOCUS       NZ_CP119750             7105 bp    DNA     circular CON 19-MAY-2024
DEFINITION  Escherichia coli strain E15 plasmid pE15-4, complete sequence.
ACCESSION   NZ_CP119750
VERSION     NZ_CP119750.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33693438
            Assembly: GCF_029319085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   zhu,Q., zhu,z., wang,C.Y., wu,L.Y., wang,W. and zhang,J.Y.
  TITLE     Genetic features of mcr-1-producing Escherichia coli isolated in
            Chinese bovine and chicken
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7105)
  AUTHORS   zhu,Q., zhu,z., wang,C.Y., wu,L.Y., wang,W. and zhang,J.Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2023) Lanzhou Institute of Animal Husbandry and
            Veterinary Medicine, Chinese Academy of Agricultural Sciences, No.
            335 along the alkali ditch in Qilihe District, lanzhou, lanzhou
            730050, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2022
            Assembly Method                   :: SPAdes v. 3.15.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 227.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029319085.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 05:44:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,374
            CDSs (total)                      :: 5,254
            Genes (coding)                    :: 4,987
            CDSs (with protein)               :: 4,987
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 267
            CDSs (without protein)            :: 267
            Pseudo Genes (ambiguous residues) :: 0 of 267
            Pseudo Genes (frameshifted)       :: 104 of 267
            Pseudo Genes (incomplete)         :: 174 of 267
            Pseudo Genes (internal stop)      :: 49 of 267
            Pseudo Genes (multiple problems)  :: 55 of 267
            Pseudo Genes (short protein)      :: 1 of 267
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119750.1.
            Bacteria and source DNA available from chicken.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E15"
                     /host="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pE15-4"
                     /geo_loc_name="China:Hebei"
                     /collection_date="2020-08"
     gene            269..>385
                     /locus_tag="P1J19_RS26755"
                     /old_locus_tag="P1J19_26755"
                     /pseudo=""
     CDS             269..>385
                     /locus_tag="P1J19_RS26755"
                     /old_locus_tag="P1J19_26755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023525214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /name="IS1595 family transposase"
     gene            complement(413..1585)
                     /locus_tag="P1J19_RS26760"
                     /old_locus_tag="P1J19_26760"
     CDS             complement(413..1585)
                     /locus_tag="P1J19_RS26760"
                     /old_locus_tag="P1J19_26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_190996207.1"
                     /translation="MKINYKLTIANATVLVCLLPYISPFKTGLDSQPYGLILIIISWLT
                     LYKHQTKHKHFMLLSLITFLLSSIILTVEAIIYGDTLSFIRGVYGYFVLFVAPFYFLTY
                     LRFYDFPKRTFYLSSSLYFIAAIIQAFVDVNFFDFLVDIRTSESRGVTSLTPEPTYYGI
                     VCLLLLMVCLVSESFKNKERKYLSLFFVIQIVFLAKSSMAILLLLILSFYFLCLKKISS
                     TKILLGLFLLLCGHFGILHLMEGSRVYDLYTRSIELGFYLFKIDASMNERLSHIYFSLY
                     GMLSNAFLPNFNSNFSTLLDNRYILSDGFFWYGESGKKIMSYWGGVFFNLGFIGVFYYI
                     ILASSVKKGQLGVFLFVLTPILFTAVPMAMTIVSFLFSIFIFRSELRKPC"
                     /name="hypothetical protein"
     gene            complement(1759..2652)
                     /gene="repC"
                     /locus_tag="P1J19_RS26765"
                     /old_locus_tag="P1J19_26765"
     CDS             complement(1759..2652)
                     /gene="repC"
                     /locus_tag="P1J19_RS26765"
                     /old_locus_tag="P1J19_26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_001669301.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRSLKRGERKRGKLDVVYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPGGLVLSPEPQTEAGQQLRLFLEPKWEAV
                     QQDAMVVKGSYRALAREIGYANPDDTKPVRECIERLWKVSIIAQNGRKRQGFRLLSEYA
                     SDEQDGKLYVALNPLIACAVMGGAQHVRIDMDEVRALQTDPARLMHQRLCGWIDPGKSG
                     RVELDTLCGYVWPGQATNPNTLKTRRQAARRALPELAALGWTVSEYAKNKFEIGRPKAT
                     HLLGPAPKTQEISHTA"
                     /name="repC"
     gene            complement(2639..3478)
                     /locus_tag="P1J19_RS26770"
                     /old_locus_tag="P1J19_26770"
     CDS             complement(2639..3478)
                     /locus_tag="P1J19_RS26770"
                     /old_locus_tag="P1J19_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_001517559.1"
                     /translation="MATHKPIDILASFTELPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGRVIYLPAEDPPVAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGNLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAISADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSGMTTGEAEE
                     WGVDDTQRPYFVRFGVSKANYGAPFKDRWFRRYDGGVLKPAVLEKQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(3685..3939)
                     /locus_tag="P1J19_RS26775"
                     /old_locus_tag="P1J19_26775"
     CDS             complement(3685..3939)
                     /locus_tag="P1J19_RS26775"
                     /old_locus_tag="P1J19_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001517558.1"
                     /translation="MKIEIELHDSVAAELAYIVELHKEHGAANAQDSVEALLAYVASAI
                     AAGSRRPGAWERTCLDMMGLVANTDEHHYYRSGYGRPEA"
                     /name="hypothetical protein"
     gene            complement(3999..5006)
                     /locus_tag="P1J19_RS26780"
                     /old_locus_tag="P1J19_26780"
     CDS             complement(3999..5006)
                     /locus_tag="P1J19_RS26780"
                     /old_locus_tag="P1J19_26780"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_309476376.1"
                     /translation="MALAGAASNRATGGHMADFQAQVKGDRTAQAIARQLKAMGCDRYD
                     IGIRDAASGKMMNREWTPQEVQQNADWLKRMNAQGNDIYIRPAEQARHGLVLVDDLSSD
                     DLDAMKQEGREPAAIIETSPKNYQAWVKVAQDAPAVHRGVIARELAREYDADPASADSR
                     HYGRLAGFTNRKDKHTSNGYQPWVLCRESSGQIATAGPELMQQAGQVLDSIKRRQEKAR
                     RLEGIEAGPKRSYRRDAVDDYRSEMAGLIKRYGDDLSRCDFIAAMKLASNGREPDEIAK
                     AMAEASPAIMDRKAGHEADYIQRTLQKVMELPQVQEARAELARQAQRKGPEPGM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            complement(4937..5347)
                     /locus_tag="P1J19_RS26785"
                     /old_locus_tag="P1J19_26785"
     CDS             complement(4937..5347)
                     /locus_tag="P1J19_RS26785"
                     /old_locus_tag="P1J19_26785"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_225883815.1"
                     /translation="MSAIDRVRKSRGINELAAEIEPLAQSMATLADEARQRIAEVQQAS
                     EEQAASWTSQQQQAMSAWREAAKDMRTAAGELTTASQTARSAARGWTWRLWVGVLIASV
                     TPILVLLIASWLWLEPQVIEQQGGIWLIFKLR"
                     /name="IncQ-type mobilization protein MobB"
     gene            complement(<5454..6095)
                     /locus_tag="P1J19_RS26790"
                     /old_locus_tag="P1J19_26790"
                     /pseudo=""
     CDS             complement(<5454..6095)
                     /locus_tag="P1J19_RS26790"
                     /old_locus_tag="P1J19_26790"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            6266..6577
                     /locus_tag="P1J19_RS26795"
                     /old_locus_tag="P1J19_26795"
     CDS             6266..6577
                     /locus_tag="P1J19_RS26795"
                     /old_locus_tag="P1J19_26795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_190996208.1"
                     /translation="MAKAAERLAKLEEKRARINAEIQRVKAREQQQKRKEETRRKVLVG
                     AWMMGKVQSGEWPEQKLIEAMDSYLERDHDRALFGLPPIQPKPQQQPEHVQYSQEVQG"
                     /name="mobilization protein"
     Typing          complement(6146..6232)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.318"
                     /coverage="98.0"
                     /evalue="5.47e-30"
     Typing          complement(2640..3478)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.929"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4003..6095)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.469"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgggggttgg gtggagttac cgtgggggtc acacatactc gcaccagatc acgctgcccc
       61 agtgctcgcc ggtttccggc tcattagaag gccgctcgtt ctctatttga ttatctgttt
      121 gttgatccgg cactgatttc tgccatcttc acctttcatc atgaggctca tttgttacca
      181 ttccgttcaa aatttttcct gattacccat gttgtgcagc catgcctata ctgtatgcgt
      241 atacagccat caagcaaagg gtgcagccat ggccaagaac aaagttcagt atcagaaggg
      301 tctgagcgta aacgattttt tggagaagta cggcacggaa gagctgtgcg agaaagctct
      361 ctttaagtgg cgttatcccc aaggcttggg tgttagctat gcacgcagaa atttagcaag
      421 gtttccttaa ttctgatctg aaaataaaaa tggaaaataa aaaacttacg attgtcatag
      481 ccataggaac tgctgtaaat aatattggtg ttaaaacgaa aaggaaaaca cctagctgtc
      541 ctttttttac actcgaagca agaattatat aataaaatac accaatgaaa cctagattaa
      601 aaaacacccc gccccagtag ctcatgattt ttttaccgga ttctccatac caaaaaaaac
      661 catcagataa aatgtaccta ttatctagta aagtagaaaa gttactatta aagtttggta
      721 aaaaagcatt acttaacatt ccatataaag agaaataaat atgcgacagt cgttcattca
      781 tgcttgcatc aatcttgaat aaataaaatc ccaattcaat tgaccttgtg tataagtcat
      841 aaactctgct accttccatt aggtgtaaaa tcccaaaatg accacaaaga agtaaaaata
      901 agcctaaaag aatttttgtc gaactaatct tttttaagca caggaaataa aaagataaaa
      961 tcaacaacaa gagtatggcc attgatgatt ttgcaagaaa tactatttgt ataacaaaaa
     1021 acaacgagag atatttcctt tctttatttt taaaggattc agatactagg cataccataa
     1081 gaagtaataa gcaaacaata ccgtaataag taggctctgg cgtaagagat gttacacctc
     1141 tagattcgct tgttctaatg tcaaccaaaa aatcaaaaaa attcacatct acgaaagctt
     1201 gaattattgc tgcaatgaaa tacaatgatg atgataaata aaatgtcctc tttgggaagt
     1261 cgtaaaacct aaggtaggtt aagaaataaa aaggagctac aaataaaaca aaatacccat
     1321 acactcccct aataaaagat aaagtatcac cataaataat ggcttctaca gttaaaatta
     1381 tactggataa aaggaatgtt attaaagata atagcatgaa gtgtttgtgc ttagtttgat
     1441 gtttataaag agttaaccat gatataataa taagtataag accgtatggc tggctgtcga
     1501 gtccagtttt aaatggtgat atgtaaggga gtaagcaaac cagaacagta gcgttagcaa
     1561 ttgtcaattt ataatttatc ttcaaaatta attaccttta tttattttaa ttagtagcat
     1621 gtgtgtttat tatactttgt ttctttatgg ttaggaacct gacgggtgct gttgtaaata
     1681 caaaactccc tccatttttg agtaaactca aagaagtcca tgccattcag tagggtgcgg
     1741 tgcttcctaa atcctccctc aggctgtatg gctaatttcc tgtgttttgg gtgctgggcc
     1801 gagcagatgc gttgctttcg gcctgccgat ttcaaacttg ttttttgcgt attcgctgac
     1861 tgtccagccc aatgcggcaa gctcaggaag cgccctgcga gccgcttggc gtcgcgtctt
     1921 gagcgtattg gggttggttg cctgccccgg ccagacgtag ccgcaaagcg tgtccagttc
     1981 cacccgcccg gacttgccgg ggtcgatcca gccgcatagg cgctgatgca tcagccgcgc
     2041 cgggtcggtc tgtagcgccc gcacctcgtc catgtcgata cggacatgct gcgcgccacc
     2101 catgacggcg caagcaatca ggggattcaa ggccacatac agctttccgt cctgctcgtc
     2161 gctggcgtac tcggacagca ggcggaagcc ctgccgcttc ctgccgttct gggcgatgat
     2221 ggacaccttc caaagccgct cgatgcactc ccgaaccggc ttagtgtcgt cggggttggc
     2281 gtagccaatt tcgcgggcaa gtgctcgata gcttcccttg accaccatcg catcctgctg
     2341 gacggcttcc catttcggct caaggaacag ccggagttgc tgcccggcct cggtctgcgg
     2401 ctcaggcgac agcacaaggc cacccggccc agccattgcc acaagtcctt gcaggatgcg
     2461 caagtcatca gcgcccagcg gctccgggcc gctgaactcg atgcgcttac cgtcgccgta
     2521 gtcatacacc acgtccagct tgccgcgctt gcgctcgcct cgcttgaggc tgcggaacag
     2581 gccgggggcc agacagtgcg ccgggtcgtg ccggacgtgg ctgaggctgt gcttgttctt
     2641 aggcttcacc acggggcacc cccttgctct tgcgctgctt ttccagcacg gcgggcttga
     2701 gcactccgcc gtcataccgc ctgaaccagc ggtctttgaa cggtgctcca tagttggcct
     2761 tgctgacacc aaagcggacg aaatagggcc gctgtgtgtc gtctacgccc cattcctcag
     2821 cctcgccggt ggtcatgccg gacaggtagg actgccagcg gatgttatcc accagcacgg
     2881 acgatccccg gctggcctgc tgctggtcgc ctgcgcccat catggccgcg cccttgctgg
     2941 catggtgcag gaacacgata gaacagccgg tatcggcgga gatggcctcc atgcggccaa
     3001 tcacttgcgc catggggccg ctggcgttct cttcctcgat gtggaaccgg cgcaaggtat
     3061 ccagcaccat caaccgtcgt ccctcagcgg ctcgcttgag gccgtcgaac cactccgggg
     3121 ccatgatgtt aggcaggttg ccgatcagcg gctggatcag caggccgtca gccacggctt
     3181 gccgttcctc ggcactgagg tgcgccccaa gggcgtgcag gcggtgatgg atggcaactg
     3241 gtgggtcttc tgcgggcagg tagatcactc ggcctgtcgg cagctcgccc acctccagca
     3301 gatccggccc gcccgcaatc tgtgcggcca gttgcagggc cagcatggat ttaccggcac
     3361 cgccaggcga taccagcgcc ccgaccgtac cggcgaccat gttgggcaaa acgtagtcca
     3421 gcggtggcgg tagctctgtg aaactcgcca gaatgtcgat aggcttatgg gtagccattg
     3481 atttgcctcc tgtgtaggcg gttgatggtc aggcgctggc ggggccgttc tccccgtcct
     3541 gcgccgccct aagttcctct aggtacagac gcagcgcctc ggcctctgcg tctgtggccc
     3601 aaatcttccg tgccttacgg cccatggctc gctgctgctc ggcaaagcgg gcctgtcgta
     3661 tagcgttagg ggtgtgttgg tctttcatgc ctcaggtctt ccataaccag agcggtagta
     3721 gtgatgctcg tcggtgttgg caaccaatcc catcatatca agacaagtac gctcccacgc
     3781 ccccggcctc cggctgccag ccgctatggc tgatgccaca taggccagta gcgcctcaac
     3841 gctatcctgt gcgttagctg cgccgtgttc tttgtgaagc tccacaatgt aagccaactc
     3901 tgccgctacg ctgtcatgta gttctatttc gattttcata acccgcccct tttccgttag
     3961 agctaacatc ataacaaaca ttagcgctaa tgcaacacct acataccagg ttcaggccct
     4021 tttctttgcg cctgcctcgc tagctcggcg cgggcctcct gcacttgcgg cagctccatc
     4081 accttctgca acgtgcgctg aatgtaatca gcttcatggc ccgccttacg atccatgatt
     4141 gcaggactgg cctcggccat ggccttggca atttcgtccg gctcccggcc attgcttgcc
     4201 aacttcatag cggcgatgaa atcgcaccta ctgaggtcgt caccgtaccg cttgatcagc
     4261 ccggccattt cgctgcggta gtcgtccacg gcgtcacggc gatagctgcg ttttggcccg
     4321 gcctcgatgc cttccagcct gcgggccttc tcctgtcggc gcttgatgct gtccagcacc
     4381 tgcccggcct gctgcatcag ctccggccct gcggtggcga tctggccgct ggactcccgg
     4441 cacagcaccc atggctgata gccgttgctg gtgtgtttgt ccttccggtt ggtaaagccc
     4501 gctaggcgtc cataatggcg gctgtcagca cttgccgggt cagcgtcata ctcgcgggcc
     4561 agctcccgag cgatcacgcc cctgtgaact gctggggcgt cctgcgccac cttcacccat
     4621 gcctgatagt tcttcgggct ggtctcgatg atggcggcag gctcccggcc ctcctgcttc
     4681 atggcgtcca gatcgtcgct gctgaggtca tccaccagca caagaccatg ccgggcctgc
     4741 tcggcagggc ggatgtaaat atcattgccc tgggcattca tgcgcttgag ccagtcggcg
     4801 ttctgctgca cttcctgcgg tgtccattcc cggttcatca tcttgccgct ggcggcgtcc
     4861 cgtatgccaa tgtcgtaccg gtcacagccc atggccttga gctgtctagc gatggcctgc
     4921 gcggtgcgat cacctttcac ctgagcttga aaatcagcca tatgcccccc tgttgctcga
     4981 ttacttgcgg ctccagccaa agccatgatg cgatcagcag caccagaata ggcgtcacgg
     5041 aagcgatcaa gactcctacc catagccgcc acgtccagcc acgggcggcg ctccgggccg
     5101 tctggctggc cgtggtcagc tccccggcgg ctgtcctcat gtccttggct gcctcccgcc
     5161 atgcgctcat ggcttgctgt tgctggctcg tccagctcgc ggcctgctcc tcgctggcct
     5221 gctgtacctc ggcgatccgc tgccgggctt cgtccgccag tgtcgccatg ctctgggcca
     5281 acggctctat ctctgccgct aactcgttga tccctctgga tttcctcact ctgtctatcg
     5341 cgctcatgct cgatggcctc ccggtattct tgtaggtcga tgatctggcc gttggccgtg
     5401 tcgatggata gggctgtgtc ggcccgctcg gtgcggatgc cccggccctc catctccacc
     5461 acgttggggc caaggtggat gcccggcagg cgctcgatgc cctgcgcctc aagggtgcgg
     5521 tggtcaatgc gggcctcgtg cccggcccgt tcaagggcgc ggttggcgtg gtggctccat
     5581 gcctcgcggg tctgctcaag ccatgccttg ggcttcaggc tttcggactt ctgcgccccg
     5641 ccccgctctg gctgtttggc gttgtaccgc ttgaaccact ggtcagccgg gcgctcgata
     5701 ccgtcgttct tgcgctcgga gatcatcagg tggcaatggg ggttctcgcc gtcaccggca
     5761 tggatggcca gcgtgtacgg caggcgctcg ccctcggtca gatgccgggc gaattcgtcc
     5821 accagctcac gctgttggtc tagggtcagc tcgacgggca gggcgaattc aacttctttg
     5881 aataggcgac cgttggcccg ctcgtacagg tcggcaacgt cccagtagtc ggcgggacgc
     5941 tcggcccact tgggcaggtg gccggactgg gtatgtaata cctcgtcacg gtcgcgggag
     6001 taacggccct cccgctggat gtaatcggct ttcgccttgg ccgactggcc gccgttccgg
     6061 ctgccggtct tggccgtaag gtgatagatg gccatagggg tttagctcca tgcaatggcc
     6121 ctcggagagc gcacagcccc gaagggcagt ttcttgaaga gaaaccggta agtgcgccat
     6181 cccgtcagaa tgacggtatg ggtttgcggc atctgtgctc cgatgatagc atgttgacaa
     6241 gcgcataacc aaggggactg tcaacatggc aaaagcagcg gaacggctcg caaagctgga
     6301 agaaaaacgg gcacggatca acgccgagat acagcgggtc aaggcacggg agcaacagca
     6361 gaagcgcaag gaggaaaccc gacgaaaagt gctggtcggc gcgtggatga tgggtaaagt
     6421 gcagagtggt gaatggcctg aacaaaagct gatagaggcc atggatagct atctggaacg
     6481 cgaccacgac cgggcactgt tcggactccc gcccatacag ccaaaacccc agcagcaacc
     6541 agaacacgtc cagtacagcc aagaggtgca agggtgagtg actggcgccg cgctgacccg
     6601 gcagaactgg cggcattcga cccggataca aaggtctgca cgatgaactg tggccctcat
     6661 gccctcgacc cgcgaagcag ggcagagagg aagttgcttt gcacggactg cgcagaagcc
     6721 cctaaaacgc ctccctgcgg ggctacacac cgcccccacc cttcgggtag gggtaaaggc
     6781 cgctaaagcg gcaaaacagc ccctgagcca accactgccc ttgatctggc accagcgggc
     6841 aaagcccgcc tttcacccta ccgcgtagcg gtggggtgga gggggtgtgt agcctagcgc
     6901 agcggataga tcagttatcc ggcctctggc cgggcatttt gggcaatgcc agcccgcgcc
     6961 gaaggggcgc gggctgtgct gcgcctagtg gagattcttg gagaatcttg gatggatttt
     7021 gcgaatcgcc agcaaatccg cgccccgtct gggttttcca gattggggcg ggtggagtta
     7081 ccgtgggggt tgggtggagt taccg
//
