LOCUS       NZ_CP119584             9313 bp    DNA     circular CON 21-MAR-2023
DEFINITION  Escherichia coli strain C012_chr plasmid pEC012-7, complete
            sequence.
ACCESSION   NZ_CP119584
VERSION     NZ_CP119584.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33688535
            Assembly: GCF_029278625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Wang,Z., Wang,S. and Guo,W.
  TITLE     Genomic characteristics of mcr-1, TEM-176 and blaCTX-M-14 in a
            single multidrug-resistant Escherichia coli ST457 from Broiler in
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9313)
  AUTHORS   Wang,Z., Wang,S. and Guo,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2023) 402, Shanghai Veterinary Research Institute
            Chinese Academy of Agricultural Sciences, Ziyue Road No. 518,
            Shanghai, Shanghai 201100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-OCT-2022
            Assembly Method                   :: HGAP v. v4; CANU v. v1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 219.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/20/2023 17:04:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,330
            CDSs (total)                      :: 5,199
            Genes (coding)                    :: 5,018
            CDSs (with protein)               :: 5,018
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 63 of 181
            Pseudo Genes (incomplete)         :: 100 of 181
            Pseudo Genes (internal stop)      :: 40 of 181
            Pseudo Genes (multiple problems)  :: 25 of 181
            Pseudo Genes (short protein)      :: 4 of 181
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119584.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C012_chr"
                     /isolation_source="liver"
                     /host="broiler"
                     /db_xref="taxon:562"
                     /plasmid="pEC012-7"
                     /geo_loc_name="China: Fujian"
                     /collection_date="2015"
     gene            join(8994..9313,1..378)
                     /locus_tag="P1J04_RS26595"
                     /old_locus_tag="P1J04_26595"
     CDS             join(8994..9243,9243..9313,1..378)
                     /locus_tag="P1J04_RS26595"
                     /old_locus_tag="P1J04_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(1363..1782)
                     /locus_tag="P1J04_RS26600"
                     /old_locus_tag="P1J04_26600"
     CDS             complement(1363..1782)
                     /locus_tag="P1J04_RS26600"
                     /old_locus_tag="P1J04_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_239170938.1"
                     /translation="MRLTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPIKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1863..2300)
                     /locus_tag="P1J04_RS26605"
                     /old_locus_tag="P1J04_26605"
     CDS             complement(1863..2300)
                     /locus_tag="P1J04_RS26605"
                     /old_locus_tag="P1J04_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_169750488.1"
                     /translation="MGHCNFMAWHTLQDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            2526..4415
                     /gene="cra"
                     /locus_tag="P1J04_RS26610"
                     /old_locus_tag="P1J04_26610"
     CDS             2526..4415
                     /gene="cra"
                     /locus_tag="P1J04_RS26610"
                     /old_locus_tag="P1J04_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin R"
                     /protein_id="WP_113771640.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGVTSTQARQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLTPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKEMGRVTERIVDAINLALILNQTE
                     SRLSESQKNVDVANQIISDTVKAINDVNKKIAEKRNQQVSLTDLMNKKQKEVEDLKKIF
                     KNHSYHRIRDAQREYDDARNKYALLASDINALQAQVSGLTARKQQAEQNKAAAEKAKAD
                     AAAKAAAEKAAAEAKAKAEAEKARKEAEEKANDEKAVLTKASEIIISVGDKAGEYLGDK
                     YKVLSREIADNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDAIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYVMKANNVREKSIEGYQTGNWGPLMLEIESWVLSGIASAVAL
                     SFFSVAVGSLFITAGVSVTAVGLMGIIFAGFIGALIDDDFVDKLNNEIIRPAY"
                     /name="cra"
     gene            complement(4443..4967)
                     /gene="cri"
                     /locus_tag="P1J04_RS26615"
                     /old_locus_tag="P1J04_26615"
     CDS             complement(4443..4967)
                     /gene="cri"
                     /locus_tag="P1J04_RS26615"
                     /old_locus_tag="P1J04_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_089619593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R immunity protein"
                     /protein_id="WP_040090309.1"
                     /translation="MQKEYTYNKDLNRMLFLITFAGIIPLIIIFITYTSNPNFYLIRII
                     FDNTQNIPSVISSYNPVMTKVMDIYGKSAPLLAFIAFIMLFKHTRTPKITDRKKIITAC
                     ILSPFLCIFYAYFFLWHNFELTTSGRPVRLMSENDLTLLIFYICLYYCSFFITYALCLQ
                     PVIAYKLWKER"
                     /name="cri"
     gene            5087..5227
                     /gene="crl"
                     /locus_tag="P1J04_RS26620"
                     /old_locus_tag="P1J04_26620"
     CDS             5087..5227
                     /gene="crl"
                     /locus_tag="P1J04_RS26620"
                     /old_locus_tag="P1J04_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="WP_072020761.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSELTGIAV
                     Q"
                     /name="crl"
     gene            complement(5445..5558)
                     /locus_tag="P1J04_RS26625"
                     /old_locus_tag="P1J04_26625"
     CDS             complement(5445..5558)
                     /locus_tag="P1J04_RS26625"
                     /old_locus_tag="P1J04_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071699851.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(6228..6419)
                     /locus_tag="P1J04_RS26630"
                     /old_locus_tag="P1J04_26630"
     CDS             complement(6228..6419)
                     /locus_tag="P1J04_RS26630"
                     /old_locus_tag="P1J04_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6484..6807
                     /locus_tag="P1J04_RS26635"
                     /old_locus_tag="P1J04_26635"
     CDS             6484..6807
                     /locus_tag="P1J04_RS26635"
                     /old_locus_tag="P1J04_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_040090312.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPLARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7016..>7447
                     /locus_tag="P1J04_RS26640"
                     /old_locus_tag="P1J04_26640"
                     /pseudo=""
     CDS             7016..>7447
                     /locus_tag="P1J04_RS26640"
                     /old_locus_tag="P1J04_26640"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            7485..8015
                     /locus_tag="P1J04_RS26645"
                     /old_locus_tag="P1J04_26645"
     CDS             7485..8015
                     /locus_tag="P1J04_RS26645"
                     /old_locus_tag="P1J04_26645"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_073534392.1"
                     /translation="MSNLLQTGAEFEKKLKERAASTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVSTMDTHWQTVLKMSVKRWLWLIIVSVLMFTTTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTE
                     DKGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            8022..8255
                     /locus_tag="P1J04_RS26650"
                     /old_locus_tag="P1J04_26650"
     CDS             8022..8255
                     /locus_tag="P1J04_RS26650"
                     /old_locus_tag="P1J04_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_073534391.1"
                     /translation="MTELEKHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRGNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          6056..6143
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.506"
                     /coverage="100.0"
                     /evalue="3.21e-32"
     Typing          complement(5487..6033)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.264"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          6798..8338
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.393"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggacgaac agtggggcta tgtcggggct aaatcgcgcc agcgctggct gttttacgcg
       61 tatgacaggc tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg
      121 ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc
      181 tggccgctgt atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg
      241 cagcgaattg agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag
      301 tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac
      361 ataaaacact atcaataagt tggagtcatt accggttaat tgttttgttt tttacaaaaa
      421 agacaaaata acagaaaata aaatctaatc atttttgttg atcgttgttg ctcggtgccg
      481 ttattcttaa gctgaaagtg aattcttcta attttttctt atattcagac gcaattaaca
      541 gcccctgctc cttgagggac ttttttttat ctttttctct ctgttttata ctgttttgag
      601 tagtaactat aaaggcagtg aagttctgtc ttatgagcgc atttctggcg gtgatgcgac
      661 taacccattc tacatcaacg aacaattcac cagaaacgcg cctgtaaatt tttagctgag
      721 caagggctct gtgtcgagag gtgtggatgt ctggttcacc attttaaaac acttcttccg
      781 gcggttttta gtttcgatgc aggccagggg gcgctgtaaa gttatggtcc ctgattactg
      841 tgccagaatt ctgagacaga caggaggtct accggttatt tttcgaattg ggcgcttccg
      901 gaagagagag tttcattgga cgttggtcgc ggatggtgag cacgtttcga tgttgtgtga
      961 cgaacattca gctcagttga tggagactgt cagaaacgcg ctcagaagtg ttgtaacgcg
     1021 atttctggta tcaggcaatt gtttcctttt tctggatcct tatacagtgt gtgtacggtg
     1081 atgagttctg tcacgcacag taagattccc ccccccagat gcttccatct ggcaggaatt
     1141 gatccggctg aaaatatgaa aaaacacccg tttaagtaat ctcaggcagg taattcgttc
     1201 gtgattcgcc tggctgcata gatatgcata agattaaaat ttatcaggcg cgatcgcggc
     1261 ggtttttcgg gtggtttgtt gccattttta cctgtctgct gccgagatcg cgctgaacgc
     1321 atttcagtgg tgcgtacaat taagggatta tggtaaatcc acttactgtc ttccctcgta
     1381 gccatcgaga taaaccgcag aaatcgtctc agccagcagc ctggatttta tcggggtaag
     1441 gtctttgctg cggtaatcag tacccgcaaa ttgagttgtg ccccggagtg agtttaaaaa
     1501 ttcttggctg tgaaactgct gaatgcgttg ttgcacatca ctccgtgatt ttcctgtcgc
     1561 tttatcttta gctcccatat cccagaactg ccgaaaaaaa ggcaccatca ttcgtgttgt
     1621 gtcggctttt tgggtggaaa agttgggatc aaaaccgtca tcagaagcat atacgtaatg
     1681 ccgggtatgt tgttccacgc tgccctttgg ggtacaggct gtgagagcta ctgttgctgt
     1741 tatcatggcg ataagacaaa gtttgtttcc ggtaagtctc atgagtgtct ccctacctta
     1801 aagtatttat tctcggtgag ttattatccg aagactattt tatcatcagt ctctgacagc
     1861 gttcagcgac cgaacaacct ggaccagaat ccccgtttct tttccagttc cagtgcctgc
     1921 ctgagctggg ctatctcatt ttgtagctgt tcccgttctg cttcctggtg tctgcgatcc
     1981 atatcctgtg cctgtttatc ctcaagcatc agcgtcaggc attgtttcag ctcattcagt
     2041 tcccggagaa tgcgttctgt ctgctgatca tgtggatttt ctgcctgtcc cacagtgtgc
     2101 ctttcttgtg tctcattttg ctttaattcg ccgtatgccc ggatcagttc actggtttca
     2161 aactcccttc gtccgtcctg gctggtacgg taactgacaa gacctgagcg catatcacgg
     2221 taaaactgtg aacgcgatct tccagtcatt ttcagtgcgt cctggagtgt gtgccatgcc
     2281 ataaagttac agtgtcccat aggtgtctca tctcatagtt tcagtaaaac ataatgaggt
     2341 ctgagaacgg taatgtttgc gccggttttt gtggtttccg gcgggaatag cgcgtggtgt
     2401 gagagactgt ttttttgatc gttttcacaa aaatggaagt ccacagtctt gacagggaaa
     2461 atgcagcggc gtagctttta tactgtacat aaaaccagtg gttatgtgta cagtatttgt
     2521 ttttaatggt ttgttttaaa agtcaaagag gaatgaatat gccggggttt aattacggtg
     2581 ggtatggtga cggtactggc tggagttctg aaagtggtgg tccagctcca ggcggtggta
     2641 tgcatggaaa tagtggaggc cagcgcggag ataacgcaaa cagtagcaat tctgtgagtc
     2701 agcagatttc tgccattcag aatgatcaga aacttaaaca gaaagtggtg aatatgctga
     2761 ttgctgcgag aaaaatgaat cctgaagcaa aaatgattct gggaagtatt gctccttctg
     2821 gtgttatgca ggtcaccatt gagggggtaa cctccactca ggccaggcag cttggtttgg
     2881 gagggctggt catgggatat aacgcttctg gtgttattgg ggctgtgggc gaaatagaca
     2941 ccggtcaccg ccttaatgca tcaggagcaa gtactcccgg aagcgaaact tctgtggaga
     3001 gttttgtgaa cggacagaaa ccagccggag aatggcatgc tgtggcgaag gacagctgga
     3061 caggggctgg tccagtgaat gtagggctgg ttaataacgc catcaaaagt gtccggatta
     3121 tcaaaaaagg ctatgtgacg ggcgttctca cgccagaaga ggtgatgaat aaagcagagt
     3181 acaaagcaat gcggcaggct ttcgattccc ttccactggc aaaacaggga gaggctgtca
     3241 gacagattgt tgctgcttgg agtctggcat atcaggattt tccggtaaat ctgaaaaaag
     3301 agatggggcg tgtcactgaa cggattgttg atgctattaa cctagcgctc attcttaatc
     3361 agacagagtc ccgtttgtcg gaatcacaaa agaatgttga tgtagcgaac cagattatca
     3421 gtgatacagt aaaagctatt aatgatgtaa ataagaaaat agcagaaaaa cgaaatcagc
     3481 aggtatcact gactgatttg atgaataaaa aacaaaaaga ggtcgaagat cttaaaaaga
     3541 tattcaaaaa tcactcttat caccgtattc gtgatgccca gagggaatat gatgatgccc
     3601 gaaataaata tgctttactg gccagtgata ttaatgcgct gcaggcacag gtttccggac
     3661 tgacggcaag gaaacagcag gcagaacaga acaaggcagc agcagaaaaa gcaaaggcag
     3721 atgcagcggc gaaagctgca gcagaaaaag cggctgctga agcgaaagca aaggctgaag
     3781 ctgaaaaagc cagaaaggaa gcagaggaaa aagcaaatga cgagaaggct gttctgacaa
     3841 aagccagtga aattatcatc agtgttggtg ataaggctgg ggagtatctg ggtgataaat
     3901 ataaagttct ttctcgtgag atagcagata atatcaaaaa ctttcagggc aaaacgattc
     3961 gtagttatga tgaggcgatg gcttctgtca ataaactgat ggctaatcct gatcttaaaa
     4021 ttaatgccgc cgacagggat gcaattgtga atgcctggaa agcatttgat gcagaggata
     4081 tggggaataa gtttgccgcg ctgggtaaga cgtttaaagc cgcagattat gtgatgaagg
     4141 caaacaacgt ccgggagaag agtatcgagg ggtatcagac cggaaactgg gggccgctga
     4201 tgctggagat tgagtcctgg gtattgagtg gtattgcttc tgctgtggcg ctatcatttt
     4261 tctcagtcgc tgttggttca ttatttatta cagcgggcgt gtctgtaacg gctgtgggac
     4321 ttatgggcat tatttttgct ggctttatcg gtgcgctgat tgatgatgat tttgttgata
     4381 agttaaataa tgaaattatt cgccctgcgt attaattgag agtcgaaagg tagtcaggag
     4441 gactaccttt ctttccagag tttatatgct attactggtt ggaggcagag ggcgtaagtt
     4501 atgaaaaaag agcaataata caggcagata taaaagataa gcaaagtaag atcgttctca
     4561 gacatcaatc gaacaggtct tcccgatgtt gttaactcga agttatgcca caagaaaaaa
     4621 taagcataaa aaatacacaa aaagggggac agtatacaag ctgttatgat tttttttctg
     4681 tctgttattt tgggggttct tgtgtgtttg aatagcatga tgaaagctat aaatgctaat
     4741 aatggtgcgc tcttaccgta gatatccatc acctttgtca tcacagggtt atatgaagaa
     4801 attactgaag gtatattttg agtattatcg aaaatgatgc ggattaaata gaagttgggg
     4861 tttgatgtat aagttataaa aataataatt aatgggatta tacccgcgaa agtaatcaaa
     4921 aaaagcatcc tgtttaaatc tttgttgtaa gtgtactctt tttgcattgg tataatactc
     4981 ccggtttttc attgttatta atttagttgt tgatgtgtat aagcatatca tgctgtgcga
     5041 gtaatggtca ccctaatatt aggggtatga gttaatgagg ggatatatga aaatgatatt
     5101 atcaggaggg atgcttgtct tgatggcttt gctatcaggc tgccaggcta attatattcg
     5161 cgatgttcag ggagggaccg tcgcaccatc ctcctcttct gaactgacgg gaatcgcggt
     5221 tcagtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5281 ttgcaaacaa aaaaaccacc gctaccaacg gtggtttgtt tgccggatca agagctacca
     5341 actctttttc cgaaggtaac tggcttggag gagcacagtc accaaaacct gtcttttcag
     5401 tttagcctta accggctcat aacttcaaga ccaactcctc taaatcagtt accagtggct
     5461 gctgccagtg gcgctttgtc gtgtcttacc gggttggact caagacgata gttaccggat
     5521 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5581 acctacaccg aactgagata ccaacagcgt gagctatgag aaagcgccac gcttcccgaa
     5641 gggagaaagg cggacaggta tccggtaaac ggcagggtcg gaacaggaga gcgcacgagg
     5701 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5761 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5821 ggcgaggcct ttcgttgccc tttgcctttt tgctcacatg ttctttccgg ccttatcccc
     5881 tgattctgtg gataaccgta ttactgcctt tgagtgagct ggcaccgctc gccgcagccg
     5941 aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     6001 tctccttacg catctgtgcg gtatttcaca ccgtatatgg cgctgttttc ttacaatccg
     6061 cactgatgct gcatatctaa gccagtatac actccgctat cgctatgtga ctgggtcagg
     6121 gctgcgcccc gacacccgct aaaacctgct gacgcgccct gacgggcttg tctgctcccg
     6181 gcatccgctc acagacaagc tgtgtccgtc tccgggagct gcatatgtca gaggttttca
     6241 ccgtcatccc cgaaacgtgc gaggcagctg cggtaaagct catcggcgtg gtcgtgaagc
     6301 gattcacaga tgtctgcctg ttcatccgcg tccagttcgt tgagcttttc cagcagcgtt
     6361 aatgtctggc ttctgataaa gcgcgccatg ttaagggcgg ttttttcctg tttagtcact
     6421 gatgcctccg tgtaaggggg gatttctgtt catggggtaa tgataccgat gaaacgcgag
     6481 aggatgctca caatacgggt tactgatgat gaacatgccc ggttacttga acgctgtgag
     6541 ggtaaacaac tggcggtatg gatgcggcgg gtctgcctgg gggagccgct tgcccgttcc
     6601 gggaaactgc cgacactggc accgccgtta ctgcgtcagc tggccgccat cggaaataac
     6661 ctgaatcaga cagcccgtaa ggtgaacagc gggcagtggt cttccggtga tcgggttcag
     6721 gtggtggccg cactgatggc catcggggat gagctgcgcc ggctgcgtct ggccgtcagg
     6781 gaacaggggg cgcgggatga tagttaaatt tcatgccagg ggaaaaggtg gtggcagtgg
     6841 tccggttgat tacctgctgg ggagggagcg taaccgcgaa ggcgcaacgg tgcttcaggg
     6901 taatccggaa gaagtccggg aactcatcga tgccacgcca tttgcgaaga aatacacgtc
     6961 aggtgttctg tcgttcgcgg agaaggagct gccgccggga gggcgtgaaa aagtgatggc
     7021 gagctttgag cgtgtactga tgcccggtct cgaaaagaat cagtacagca tcctgtgggt
     7081 agagcaccag gacaagggac ggcttgagct gaattttgtc attccgaaca tggagttaca
     7141 gaccggaaaa cgcctccagc cgtactacga ccgggcagac aggccgagaa ttgatgcctg
     7201 gcagacgctg gttaatcacc attacgggct gcatgacccc aacgccccgg agaaccgccg
     7261 gatactgact ctccctgata acctgccgga aacgaaacag gcgcttgctg agggcgtcac
     7321 acgaggtata gatgcacttt accatgccgg agagataaaa ggtcgtcagg atgtgattca
     7381 ggcgctcacg gaagcggggc ttgaagtggt cagagtgacg cgaagcagca tcagcattgc
     7441 agacccgaac ggcgggaaga atatcaggct gaaaggagca ttctatgagc aatcttttac
     7501 agacgggcgc ggagttcgag aaaaagctga aagagagagc cgcatctaca gagaaaatgc
     7561 tgaacgacga gttcaggaag ctcgaagaat ctgtaaacag ggaactgacc tcaaacgaat
     7621 ctttaatcag gaacgctatc aacgaccata caacagccct gaaagaactt ctggagaggt
     7681 atcagaaaac gaccgtcagt acgatggaca cacactggca aaccgtgctg aaaatgagcg
     7741 tgaaacgctg gctgtggctg attatcgtct cagtgctgat gtttaccacg accgggagtc
     7801 tgctgtggta ccagggcatg aagataaacg caaacatgaa tattctccgc gagcagaaag
     7861 agagtctgga gaagctgaac gcgaagacat ggggcgtgcg ttatcacgag gacagcaacg
     7921 gacgttttct ggtgttgccg aaggggatga aagccgaaac gaactggacg gaggacaaag
     7981 gcaaactgaa cgcagtgaga ctggtacagg agtgacagag catgacggag ctggaaaaac
     8041 atttgctgaa cgcattagag caactgcaac aggactatat gcagcggctg aacgaatggg
     8101 agagcgcctt cgtggaattg cagaaaatgt tttcgcttac gcaacggggc aacgcgatgc
     8161 tgaacgagcg ggtcatgcag ttgagtcagc aggtgcagca cttgagcgag cggacagagc
     8221 gcttgagcca gttatacagc gagaacagga gataagggat gaacggctga tgcaggaacg
     8281 cgaacatgag ttatctctgg aacgggagcg gacgctggat ggcccttcgc tgggatggtg
     8341 actgaaagtc cggttgagtc gtaaacagaa tattttccag aaatgtcagg ctgcctattg
     8401 gtaatataac cagtcgttct tgcggtttat tgatttttgt acaggttgtt gctgtttttt
     8461 tttgtaaaga ggacagggat ttttctgaaa atgaacttgc gggaatgcgc tgattatgta
     8521 aaaaatataa ttgcgtatct ccgatatgtt tgtaataccg atgtggacac agcgaatctg
     8581 gaaggggtcg gagatgcgcg ttctctcatg tgttaatatt gtgttcttat attgtgatgt
     8641 gaatattttt tattttttct ttgcatcttg gaaacatgca aattatattc tgatatgcaa
     8701 aagtcaggac tagacatact atccacctat tcagttctta ccttacgccc tcactaagtt
     8761 cctgccgtga gggctttttt catgggtgtg aaaaaaggaa gccccacctg acggaggggc
     8821 tgagtgatac agagcaaaaa aatatcctgg tgttggtcac ctctggagag taataagatt
     8881 aaccataaag cgaactataa acaagaatat tgatgccagg aattttctgg gttaattggg
     8941 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
     9001 ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
     9061 gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt
     9121 tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg
     9181 gcgttggatg ccgggcaacc gcccgcatta tgggcgttgg cctcaacacg attttccgcc
     9241 atttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat
     9301 cgtctgcgcg gaa
//
