LOCUS       NZ_CP119557             3123 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Providencia stuartii strain CAVP450 plasmid pCAVP450-3123, complete
            sequence.
ACCESSION   NZ_CP119557
VERSION     NZ_CP119557.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33687284
            Assembly: GCF_029278185.1
KEYWORDS    RefSeq.
SOURCE      Providencia stuartii
  ORGANISM  Providencia stuartii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Providencia.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Patel,A., Salomon,M., Barry,K., Parikh,H.I., Elliot,Z.S.,
            Fleece,M.E. and Mathers,A.J.
  TITLE     Management of PER-1 and NDM-producing Providencia stuartii
            bacteremia in a patient with a severe beta-lactam allergy: the
            increasing need for aztreonam-avibactam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Patel,A., Salomon,M., Barry,K., Parikh,H.I., Elliot,Z.S.,
            Fleece,M.E. and Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-2023) School of Medicine, University of Virginia,
            409 Lane Road, Charlottesville, VA 22908, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology :: Illumina MiSeq + Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029278185.1-RS_2024_03_11
            Annotation Date                   :: 03/11/2024 12:51:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,311
            CDSs (total)                      :: 4,204
            Genes (coding)                    :: 4,121
            CDSs (with protein)               :: 4,121
            Genes (RNA)                       :: 107
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 33 of 83
            Pseudo Genes (incomplete)         :: 60 of 83
            Pseudo Genes (internal stop)      :: 17 of 83
            Pseudo Genes (multiple problems)  :: 19 of 83
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119557.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Providencia stuartii"
                     /mol_type="genomic DNA"
                     /strain="CAVP450"
                     /isolation_source="human"
                     /host="Homo sapiens"
                     /db_xref="taxon:588"
                     /plasmid="pCAVP450-3123"
                     /geo_loc_name="USA: Virginia"
                     /collected_by="University of Virginia Health System"
     gene            470..1456
                     /gene="mobV"
                     /locus_tag="P2E06_RS21520"
                     /old_locus_tag="P2E06_21520"
     CDS             470..1456
                     /gene="mobV"
                     /locus_tag="P2E06_RS21520"
                     /old_locus_tag="P2E06_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_276123118.1"
                     /translation="MAFAIMRAKKLNSMGTVAAALQHCYRDRETPNADQERTPDNVHLA
                     ARSTDEAMGQLRERLPEKRRKDAVLAVEYVMSASPEWWQTASADQQREFFKRSTEWLAD
                     KYGAENVVTATIHRDELTPHLSAFVVPRTADGRLSAKEFIGNRTKMSQDQTSYAERVAD
                     LGLERGIEGSRATHQRVKTHYGAIQQAGRDVPHLTPDELKPQKVKGVSLAEKVFGAVET
                     VEGVAQRLNAKIMGSVQPMAEKAAVSAQNERRAKELRETLAQQQKRLQALQEPFKGLSK
                     DQVAGLIRQAVKLRQENEQEKQERAQQIKERFKAKRERERSDRSRGR"
                     /name="mobV"
     gene            complement(1453..1608)
                     /locus_tag="P2E06_RS21525"
                     /old_locus_tag="P2E06_21525"
     CDS             complement(1453..1608)
                     /locus_tag="P2E06_RS21525"
                     /old_locus_tag="P2E06_21525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001736727.1"
                     /translation="MPVNPRDYCQPLHPGCLGLVESGLGLVESGLMLVKQWFKVVQGWL
                     KLVEKG"
                     /name="hypothetical protein"
     gene            complement(1691..1912)
                     /locus_tag="P2E06_RS22090"
                     /old_locus_tag="P2E06_21530"
     CDS             complement(1691..1912)
                     /locus_tag="P2E06_RS22090"
                     /old_locus_tag="P2E06_21530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001251694.1"
                     /translation="MAMSMYYIKTGLEYWKPKGYGYTKSREEAGVFTLKDMEELNLDGC
                     TLERAEVAFLDEDGPSPDLPHRVTLPRD"
                     /name="hypothetical protein"
     gene            complement(1903..2106)
                     /locus_tag="P2E06_RS22095"
                     /old_locus_tag="P2E06_21535"
     CDS             complement(1903..2106)
                     /locus_tag="P2E06_RS22095"
                     /old_locus_tag="P2E06_21535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000131876.1"
                     /translation="MTDPLKALFGKPDYSHIVRDTTATISITAAEMAAVLEAYDRGIDT
                     LDGTTRTALDSVISKLKDEVWP"
                     /name="hypothetical protein"
     gene            complement(2103..2651)
                     /locus_tag="P2E06_RS21540"
                     /old_locus_tag="P2E06_21540"
     CDS             complement(2103..2651)
                     /locus_tag="P2E06_RS21540"
                     /old_locus_tag="P2E06_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_257766363.1"
                     /translation="MQSVTVATETQRRIRQRELAAADEKEAQEREEARKNKGFTQVYSK
                     GWRRVRELSKGNAGAAGLYAFFAEHIDPTCGAVVCDQQFLADHLEVSTRTIRNWLKYLE
                     TERALVRIPVAGRVCAYALDPHEVWKGYDNAKEYAAFVTKTLVNKDGDIKRRIMSMFSP
                     EERPDDRDTDTLDMFGDQP"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 gctttcgcgg catatcctca ggcgctgcgc gccttgcggt attccacgac cccttgacgc
       61 tgcctccact cggaacggaa tcaggtcaag ggcgggagcg gaagccgcag ccctggagcg
      121 aagcggaagg gatcgggtga gcacccggcg aagcgaaccc ttgacgtgat ggagtgaccc
      181 ccacgctcag gcaagggggc aggtagagca gagcgaagcg gcgcggccac ctgcacccct
      241 gccacgtggc gaacgcgggg tttggggcag cgccccaagg tgttcgcaac gaccgaaggg
      301 aggcgcaggg cgaagcccgt agagcaaggc cgcaggccgc tgcaaccgcg tagcggtcac
      361 gtactttacg aagtaaagtc tagtgagtac acttcaaaga agtggcccgc cggaggcaca
      421 ttcgtacctc gacgggctgg cagcaatctg acagtgcgga gggggacgga tggcgtttgc
      481 gatcatgagg gcgaagaagc tcaacagcat gggcacggtg gcggctgcgt tgcagcactg
      541 ctaccgcgac cgcgagacgc cgaacgccga tcaggagcgc acaccggaca acgttcacct
      601 ggcggccaga tcgaccgatg aggcgatggg ccagctcagg gagaggctgc cggagaagcg
      661 ccgcaaggat gcggtgctgg cggtcgaata cgtgatgagc gcctcgccgg agtggtggca
      721 gaccgcaagc gccgaccagc agcgggagtt cttcaagcga tcgaccgagt ggctggccga
      781 caagtacggc gctgagaacg tggtcacggc cacgatccac cgggatgagc tgaccccgca
      841 cctgtcggcg ttcgtggtgc ctcggacggc tgacgggcgc ctgagtgcca aggagttcat
      901 tggcaaccgg acgaagatga gccaggacca gaccagctac gccgaacggg tggccgatct
      961 ggggcttgaa cggggcatcg agggcagcag ggccacacac cagcgagtta aaacccacta
     1021 tggggcgatc cagcaggcag gccgagacgt gccacacctg acgcctgacg agctgaaacc
     1081 ccagaaggtc aagggggtgt cactggccga gaaggtgttc ggagccgtgg agacggtcga
     1141 aggggtggcg caaaggttga acgccaaaat aatggggtct gtgcagccga tggccgagaa
     1201 agccgctgta agcgcccaga acgagcgaag agccaaggag ctaagggaaa ccctagccca
     1261 gcagcaaaaa cgcttacagg cccttcagga gccgtttaag gggctatcca aggatcaggt
     1321 agcgggactg atccggcagg cggtgaaatt gcggcaggag aacgagcagg agaagcagga
     1381 gcgcgcccag cagatcaaag agcgattcaa ggccaagcgg gaacgggagc gcagcgaccg
     1441 cagcaggggc cgttaaccct tttcaaccaa ctttaaccaa ccttgaacaa ccttaaacca
     1501 ttgtttaacc aacattaaac cactttcaac caaccctaaa ccactttcaa ccaaccctaa
     1561 gcaaccgggg tgtaagggtt ggcagtaatc gcgcggattt acaggcacgt cggatatgtt
     1621 tacaaaaact ccgtttttga cacatatccc gtgccaaagg gtgccccttt gaaaccctcc
     1681 agcgccccgc tcaatcgcgg ggcagggtga cgcggtgggg taggtcggga ctggggccgt
     1741 cctcgtccag gaacgccact tcggcgcgtt ccagggtgca gccatcgagg ttcaattctt
     1801 ccatgtcctt gagggtgaaa acccccgcct cctcgcggct cttggtgtac ccgtagccct
     1861 tgggtttcca gtactccaga cctgtcttga tgtaatacat gctcatggcc atacctcgtc
     1921 cttgagtttc gatataacgg aatccagcgc cgttctggtg gtgccgtcca gcgtgtctat
     1981 acccctgtcg taagcctcca aaacagcggc catttcagcg gctgtgatgc tgatagtggc
     2041 cgtggtgtcc cttacgatat gggagtaatc aggcttgccg aaaagggctt tgagtggatc
     2101 tgtcatggct gatctccaaa catatcaagc gtgtctgtat ccctgtcgtc tgggcgttcc
     2161 tctgggctaa acatcgacat gatccggcgc ttaatatcgc cgtccttgtt gaccagcgtc
     2221 ttggtcacga aggccgcgta ttctttggcg ttgtcgtacc ctttccagac ctcgtggggg
     2281 tcaagagcgt aggcgcaaac ccgcccagcg actgggattc tgaccaaggc gcgttctgtc
     2341 tcaaggtact tgagccagtt ccgaatcgtg cgggtgctaa cctccagatg gtcagccagg
     2401 aactgctgat cgcagacaac agcgccacag gtagggtcga tgtgttcagc gaagaaggca
     2461 tacaggccag cggcccccgc attgcccttt gacagctccc tgacgcgccg ccatcccttt
     2521 gaatatacct gtgtgaaccc cttgttcttt cgcgcctcct cgcgctcctg agcctccttc
     2581 tcgtctgctg ctgccagctc cctttgcctg attcgtctct gtgtctctgt cgctacggtg
     2641 acgctttgca ttgttgacct cctaaccgga aacagggcgt ccgcttaacc ggacgtttaa
     2701 ttcctaccat aggaaaatta tttcccaaaa ccgtttccca agtcaagcaa tttgtttcct
     2761 atgataggaa gtaagcggaa acaagctgct tccggttgtc cggaacccta cgtttcctat
     2821 cacaaaattt aaaaactata taaatcaatc atttagtatt tttccccttc tcctctaagg
     2881 gactgccgtc ccgccggcgc ttcgcttggc accctgctga cggtcgctgc gctcccttgg
     2941 agttaatgcc tcgccagagg cgagagggtt tgtttccgct gcgctaggcc caaaggggct
     3001 ttgcccctct ggactcccca cactcgccgc cgtgaggctc gggggctggg gcctgcggtc
     3061 gctgcgctcc cttcgttccc aaccccagag ccagaggctg ggtcgttccg gcagcgtcaa
     3121 ggg
//
