LOCUS       NZ_CP119555             3945 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Providencia stuartii strain CAVP450 plasmid pCAVP450-3945, complete
            sequence.
ACCESSION   NZ_CP119555
VERSION     NZ_CP119555.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33687284
            Assembly: GCF_029278185.1
KEYWORDS    RefSeq.
SOURCE      Providencia stuartii
  ORGANISM  Providencia stuartii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Providencia.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Patel,A., Salomon,M., Barry,K., Parikh,H.I., Elliot,Z.S.,
            Fleece,M.E. and Mathers,A.J.
  TITLE     Management of PER-1 and NDM-producing Providencia stuartii
            bacteremia in a patient with a severe beta-lactam allergy: the
            increasing need for aztreonam-avibactam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Patel,A., Salomon,M., Barry,K., Parikh,H.I., Elliot,Z.S.,
            Fleece,M.E. and Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-2023) School of Medicine, University of Virginia,
            409 Lane Road, Charlottesville, VA 22908, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology :: Illumina MiSeq + Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029278185.1-RS_2024_03_11
            Annotation Date                   :: 03/11/2024 12:51:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,311
            CDSs (total)                      :: 4,204
            Genes (coding)                    :: 4,121
            CDSs (with protein)               :: 4,121
            Genes (RNA)                       :: 107
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 33 of 83
            Pseudo Genes (incomplete)         :: 60 of 83
            Pseudo Genes (internal stop)      :: 17 of 83
            Pseudo Genes (multiple problems)  :: 19 of 83
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119555.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Providencia stuartii"
                     /mol_type="genomic DNA"
                     /strain="CAVP450"
                     /isolation_source="human"
                     /host="Homo sapiens"
                     /db_xref="taxon:588"
                     /plasmid="pCAVP450-3945"
                     /geo_loc_name="USA: Virginia"
                     /collected_by="University of Virginia Health System"
     gene            complement(join(3694..3945,1..684))
                     /locus_tag="P2E06_RS21475"
                     /old_locus_tag="P2E06_21475"
     CDS             complement(join(3694..3945,1..684))
                     /locus_tag="P2E06_RS21475"
                     /old_locus_tag="P2E06_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_072206570.1"
                     /translation="MATYHLSVKFGGKGKALAHANYITREDKFSQRKDLEHTEHGNMPE
                     WAKDDPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLELVRDFVKQETGGQHTYS
                     FAIHNPQASIDGGEQPHAHIMMSQRVDDGIKRSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKAQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            852..1238
                     /locus_tag="P2E06_RS22085"
                     /old_locus_tag="P2E06_21480"
     CDS             852..1238
                     /locus_tag="P2E06_RS22085"
                     /old_locus_tag="P2E06_21480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058671329.1"
                     /translation="MRISNVEWLKKRIGFIRKLGEKTTRQRQIIDLLDDEASLSETDRR
                     LLHVLATAEKNELQEQDAAQKKAVQARISGQKNRRERNHKLFLAAGLMIDAGLVDTKTG
                     ELNFDSEKLLTALKRVRCDLETSV"
                     /name="hypothetical protein"
     gene            complement(1827..2210)
                     /locus_tag="P2E06_RS21485"
                     /old_locus_tag="P2E06_21485"
     CDS             complement(1827..2210)
                     /locus_tag="P2E06_RS21485"
                     /old_locus_tag="P2E06_21485"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_113421955.1"
                     /translation="MFFYRFITLIFGAVYIATTLTLGESALSSSLYAIGIAKTPSSASG
                     ILTLLLMLWVLVVSVMMPKSWKVFAGIYLCLGLSGFLMPKSMHYGAGVYPLINSLTLLS
                     YLAFTLAIKLRDKHISKINQKTA"
                     /name="hypothetical protein"
     gene            complement(3161..3691)
                     /locus_tag="P2E06_RS21490"
                     /old_locus_tag="P2E06_21490"
     CDS             complement(3161..3691)
                     /locus_tag="P2E06_RS21490"
                     /old_locus_tag="P2E06_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     Typing          complement(2274..2429)
                     /name="Col3M"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.089"
                     /coverage="100.0"
                     /evalue="2.29e-71"
     Typing          complement(3698..3945)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.98e-45"
ORIGIN
        1 cctctggctg tcgtccagtc gtccggctgc ttcaaaaccg agatgctgtt ccggtttccg
       61 gtctataccc tgtgctttca gtgaccggct atcaataaaa ccctgctcaa agccatatcg
      121 tttcatatgc gcgttgtgcg tacgctccca gcgctcccgc agggctttca gtgcctgttt
      181 ttgttctgta ggggttaaat tatgccctga gtccttgcgc gcgccgccac gcgccggaaa
      241 tttggcgtta tagcgcctga aatactgctc tgggctgcgc ttaataccgt catcaacacg
      301 ctggctcatc atgatatggg cgtgcggctg ttcgccgccg tcaatgctgg cttgcggatt
      361 gtgaatcgca aacgaatacg tatgttgacc gcccgtttcc tgcttcacaa aatccctgac
      421 aagctccagc cgctgcgctt cgttcagttc acggggtagt gctatctcga tttcccggta
      481 cgttgaaccg ttggcacgtt cgaacgcatc cgcagcctgc cagaaatggg cggggtcatc
      541 tttcgcccat tccggcatat tgccgtgctc ggtgtgttcg agatcttttc gctgactgaa
      601 cttatcttcc cgcgtgatgt aattggcatg agcaagggct ttacccttgc cgccaaattt
      661 cacgctgaga tgataggttg ccatgatgtt tttgcccttg gttttacgca cgtgtttctg
      721 cccgcaggga gaaacatata agtgcgcttt actctaattt gaagaattat gagtaaagat
      781 aagtcatgcg acttctaacc gcaagttata tactttcacc atcggaaata tcgggatggg
      841 gaagttaaac gatgcggatc agtaacgtcg aatggcttaa aaagcgcata ggcttcatca
      901 gaaaactggg ggagaaaaca acacggcaac ggcagattat cgacctgctg gatgatgagg
      961 cttcgctcag tgagactgac aggcgactgc tccacgttct ggcaaccgct gaaaaaaacg
     1021 agttgcagga acaggatgcc gcgcagaaaa aggcggttca ggcacggatt tcaggtcaga
     1081 aaaaccgcag ggaacgtaat cacaaactgt tcttggctgc cggactgatg attgacgcgg
     1141 gactggttga tacaaaaacg ggtgagttga attttgactc tgaaaaatta ctgaccgcgc
     1201 tgaaacgggt acgctgtgat ctggaaacat cggtgtgacc gtgcattctg caaggattca
     1261 gtgatatgac gcttcctcga tgcaaaacca gtttgcatct tgcggcgagc gttgcagatt
     1321 gaatttgtgg ggtttatcag gttccagatg taacaaagcc ccgtgacagc gtcatggggc
     1381 tctgatactc taaatcggtg catacattgc tacgatttcg aattcgcact ccaatcttag
     1441 caatgggaag ctcgcttgtc acgacttcct taaaaccatt caacgacatt aacacactat
     1501 gcaccacttg attgactcgg acttagcccg gtctccgaaa ggataaacag gcaaattgtg
     1561 gtagtaaaac gtaccggaaa acgaaattcc ggctctgcct ccgaatcacg ctgcggtatc
     1621 gaaagattac agtgcgtacc tgataagagc accaatcaac ggactaggga tttcttggat
     1681 tgcaccgtcg aaagaagccc aacctaatca gacttagcta aatacgccag ctgagaagcc
     1741 atgacccaat aactaatcat ggagaaaagc aaagacaaaa agtcgttgtc gcttcgctcc
     1801 gttttgatta gtaccacatt ggggcattag gcggtttttt gatttatttt ggagatgtgt
     1861 ttgtctctta gctttattgc aagggtaaaa gccagataac taagaagtgt taaagagttt
     1921 atcaatgggt aaacaccggc accataatgc atgcttttcg gcataaggaa cccagacaaa
     1981 cccaagcata gataaatgcc agcaaaaact ttccaagatt taggcatcat gaccgaaaca
     2041 actaaaaccc acaacatcaa caaaagagtc aaaataccag aagcggagct aggggtttta
     2101 gcaatcccta ttgcataaag cgaagaggat aacgccgact ctcccaatgt tagagttgta
     2161 gcaatataca ctgccccaaa aattaaagtg ataaatcgat agaaaaacat aatagcccgt
     2221 ccacattaaa taattgtaga ttttacatga aaataggttc ttctaatccc ttttatgtta
     2281 aacgggcgag ttagttagcg aaaaccgccc gttcatcccc actaaagccc ctcaaaacga
     2341 ttttaagaca ctatcaccct ataaaacacc caaatctacg ctgaaagttt agaacggttt
     2401 tgcgagcttc tgagtgcgtt tttaacatca ttcaaactga caagtaaaac gatgcgaaat
     2461 cgctgaaaga cacaaacttg ttttgtggca agaagcgatt tgaccaaccg agtacgggtc
     2521 cgcttggaaa aaggaaatta tcctacgtta agcgttttaa gctaacgggt gatttcgttc
     2581 cattgagcgc gaacccctga ccgtaaggga tgtacgaaat gatccggctg tccgttaatg
     2641 gttttgctta ctttcggaca tgttgtctac atgctgtacg taatcgttcg gtttcggaca
     2701 cagaaacgct gtagccccgc gaaccgagtg atatccaata gccgattggt taaaaagtag
     2761 tcttagcgta ggttattttc ccttttccaa gcggacccca aaatcaattt atataatctc
     2821 attatgctct taaaaaagaa tcaacaaaaa gatccttagc agatacttac gcctgtggca
     2881 ccgatttacc ataacgcgtc attacgcgca ccgctgaaac gcgctcaggg cggttttacg
     2941 tcctgagcca taaaaacccc cgtcttctca ccgaaaaccg ctcagaatcg cataccgtga
     3001 ttttcagccc catgcataac catgcacctg actgactggc gagaacttcc ggcctgaatt
     3061 caggccggaa gtctcaaaag acgccccaaa cccggctgaa aacggtgaac gggtgcgcaa
     3121 tttattgtgc gaacgtgagg cgttggacaa gcgcagcgcg tcagttcttt ttcttttccg
     3181 gtttctgttc cgttgtcagc agcttttcaa ggtctgccac tttctgatag acggcagcag
     3241 acgcgttgta attgtcctgt agttccgttt tcacaaacca gcttgtggca aatatcagta
     3301 cgacaaaggc aaccgcaacc cctgcaaacc acttccagct gtaaccccgc agcgtgttgt
     3361 actccgcgat gatattgctg cggataatgg aaatttcctt gtccagcgcg tccgcattga
     3421 ttttcatgtt cccgacggat ttttccagac gctgaaaatc agcgctgata cgctcgccaa
     3481 tcagccccgc aacatccatt ttctgcactt cctgccggac ttcatcccgt acctgctgtg
     3541 ccacactgtc tgatatttcc cggtagcctt tcagggtttc ccttgccacc gccagcgaat
     3601 ccgccacggc tttctgctgc tcttttgcgg cctctttcag atgctccgcc gacagcagga
     3661 gtgtgagaag tttttcttcg gtgctcgcca tagttacctg cttatggaaa atccgcgtga
     3721 cagggatttc acgcgctcta tttcaacatt tccctgtttt gtgacccgtg cagccatctc
     3781 ccgcccgtac tgctgcgaga tatcatgcgt cagagatctg accagttcct tccggtttac
     3841 ctcgataccg ctggcattca tccagctacg gtcgctgtgg tcgatatgct tcgcctgttc
     3901 aggcacatcc acgactgaaa acgtccggct gtctttaata tcctg
//
