LOCUS       NZ_CP119554             8132 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Providencia stuartii strain CAVP450 plasmid pCAVP450-8132, complete
            sequence.
ACCESSION   NZ_CP119554
VERSION     NZ_CP119554.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33687284
            Assembly: GCF_029278185.1
KEYWORDS    RefSeq.
SOURCE      Providencia stuartii
  ORGANISM  Providencia stuartii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Providencia.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Patel,A., Salomon,M., Barry,K., Parikh,H.I., Elliot,Z.S.,
            Fleece,M.E. and Mathers,A.J.
  TITLE     Management of PER-1 and NDM-producing Providencia stuartii
            bacteremia in a patient with a severe beta-lactam allergy: the
            increasing need for aztreonam-avibactam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Patel,A., Salomon,M., Barry,K., Parikh,H.I., Elliot,Z.S.,
            Fleece,M.E. and Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-2023) School of Medicine, University of Virginia,
            409 Lane Road, Charlottesville, VA 22908, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology :: Illumina MiSeq + Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029278185.1-RS_2024_03_11
            Annotation Date                   :: 03/11/2024 12:51:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,311
            CDSs (total)                      :: 4,204
            Genes (coding)                    :: 4,121
            CDSs (with protein)               :: 4,121
            Genes (RNA)                       :: 107
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 33 of 83
            Pseudo Genes (incomplete)         :: 60 of 83
            Pseudo Genes (internal stop)      :: 17 of 83
            Pseudo Genes (multiple problems)  :: 19 of 83
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119554.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Providencia stuartii"
                     /mol_type="genomic DNA"
                     /strain="CAVP450"
                     /isolation_source="human"
                     /host="Homo sapiens"
                     /db_xref="taxon:588"
                     /plasmid="pCAVP450-8132"
                     /geo_loc_name="USA: Virginia"
                     /collected_by="University of Virginia Health System"
     gene            1..840
                     /locus_tag="P2E06_RS21415"
                     /old_locus_tag="P2E06_21415"
     CDS             1..840
                     /locus_tag="P2E06_RS21415"
                     /old_locus_tag="P2E06_21415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="P2E06_RS21420"
                     /old_locus_tag="P2E06_21420"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="P2E06_RS21420"
                     /old_locus_tag="P2E06_21420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            1973..>2389
                     /locus_tag="P2E06_RS21425"
                     /old_locus_tag="P2E06_21425"
                     /pseudo=""
     CDS             1973..>2389
                     /locus_tag="P2E06_RS21425"
                     /old_locus_tag="P2E06_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /name="dihydropteroate synthase"
     gene            2747..3769
                     /locus_tag="P2E06_RS21430"
                     /old_locus_tag="P2E06_21430"
     CDS             2747..3769
                     /locus_tag="P2E06_RS21430"
                     /old_locus_tag="P2E06_21430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821153.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]; GO:0008324 - monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_000083351.1"
                     /translation="MSQSHAHDHGHDHDHDHTPTVTSANERKVLVSFFLIFGFMLVEAV
                     GGVLSGSLALLADAGHMLTDAVALALAYAAFRFGRRAADSKRTFGYLRFEVIAGFLNAV
                     TLFAIVAWIAYEAWERLQAPPVILAGPMMIVAVLGLLINVLVLWIMTRGETDHVNVKGA
                     ILHVMGDLLGSVGAIVAAIVIYFTGWTPIDPILSVLVAALILRSAWKLLAKSIHILLEG
                     APEDASPEKVEQGLMSTVPGLAAVSHVHVWQLTSGRTMATLHVRPNVDEEARAVVKRVE
                     VVLREQFSIEHATVGIDWNSEADENICSLQPTTDRQDHSGHGHSEHNHDHDHSAPGHKH
                     "
                     /name="cation diffusion facilitator family transporter"
     gene            3813..4190
                     /locus_tag="P2E06_RS21435"
                     /old_locus_tag="P2E06_21435"
     CDS             3813..4190
                     /locus_tag="P2E06_RS21435"
                     /old_locus_tag="P2E06_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821152.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000203935.1"
                     /translation="MTSRKTTSSAIAHCSTSSHGATTDVLALSQSDVAVLAETFRLLGD
                     QSRLKILLQCMRGSVAVGDIADSLDLSQSLVSHHLRLLRGARLVRGERQAKHIFYGIAD
                     QHVSQVLQDMAVHISEDRNDD"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(<4295..4456)
                     /locus_tag="P2E06_RS21440"
                     /old_locus_tag="P2E06_21440"
                     /pseudo=""
     CDS             complement(<4295..4456)
                     /locus_tag="P2E06_RS21440"
                     /old_locus_tag="P2E06_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(5010..5294)
                     /locus_tag="P2E06_RS21445"
                     /old_locus_tag="P2E06_21445"
     CDS             complement(5010..5294)
                     /locus_tag="P2E06_RS21445"
                     /old_locus_tag="P2E06_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            5493..>6134
                     /locus_tag="P2E06_RS21450"
                     /old_locus_tag="P2E06_21450"
                     /pseudo=""
     CDS             5493..>6134
                     /locus_tag="P2E06_RS21450"
                     /old_locus_tag="P2E06_21450"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            6241..6654
                     /locus_tag="P2E06_RS21455"
                     /old_locus_tag="P2E06_21455"
     CDS             6241..6654
                     /locus_tag="P2E06_RS21455"
                     /old_locus_tag="P2E06_21455"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            6753..7622
                     /locus_tag="P2E06_RS21460"
                     /old_locus_tag="P2E06_21460"
     CDS             6753..7622
                     /locus_tag="P2E06_RS21460"
                     /old_locus_tag="P2E06_21460"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            7683..7895
                     /locus_tag="P2E06_RS22080"
                     /old_locus_tag="P2E06_21465"
     CDS             7683..7895
                     /locus_tag="P2E06_RS22080"
                     /old_locus_tag="P2E06_21465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            7897..8103
                     /gene="urfF"
                     /locus_tag="P2E06_RS21470"
                     /old_locus_tag="P2E06_21470"
     CDS             7897..8103
                     /gene="urfF"
                     /locus_tag="P2E06_RS21470"
                     /old_locus_tag="P2E06_21470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             1974..2488
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="63.1"
     Typing          5249..5404
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          5494..7619
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct tatgctattc
     1861 tgctcctgca gctaatggat caccgcaaac aggttactcg cctggggatt ccctttcgac
     1921 ccgagcatcc gtatgagact catgctcgat tattattatt atagaagccc ccatgaataa
     1981 atcgctcatc attttcggca tcgtcaacat aacctcggac agtttctccg atggaggccg
     2041 gtatctggcg ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg agggggcaga
     2101 tgtgatcgac ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt cgtccgacac
     2161 agaaatcgcg cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca ttcccgtctc
     2221 gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct
     2281 caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga aatcatctgc
     2341 caaactcgtc gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg gtcatgggct
     2401 taccatcgac cttcattgtc atgtctctga tcggcttggc cggcgtggtg ctggcgctct
     2461 ggctgtggcc ggccagcgac ccttctgacg tggtgcatga ccaccccagc ttgccgccgg
     2521 atcaccctca tttacgcaca cacgcagacc gaggcaagca ccatcaccag ctgattgtgg
     2581 atgacctgca ccgtatctgg ccgaaaggat aggcacattt tctcgttgcg gcaccacaac
     2641 gtatggcttc ggcggctggg aagggtgagc gaaaatactt cacatgaaca tgtattcatg
     2701 tgttatattt tcaccatagt caaacactca accagcagag gcgcccatgt cccaatccca
     2761 tgcacatgac catggtcacg accatgatca cgaccacacc cccacagtga ccagcgccaa
     2821 cgagcgcaag gttctggttt ccttcttcct gatcttcggc ttcatgctcg tggaagctgt
     2881 cggcggcgtg ctgtcgggct cgctagccct acttgccgat gctggccaca tgctgaccga
     2941 cgccgtggca cttgccctgg cttacgctgc cttccgattc ggtcgccgcg cagccgacag
     3001 caaacgcaca ttcggttacc tgcgcttcga ggtcatcgcc ggcttcctga acgccgtgac
     3061 cctgttcgcg atcgtcgcat ggatcgccta cgaggcgtgg gagcggctgc aggcgccgcc
     3121 cgtcatcctc gccggcccga tgatgatcgt cgccgtgctc ggcttactga tcaacgtcct
     3181 cgtgctgtgg atcatgaccc gcggcgaaac cgatcacgtc aatgtgaaag gtgccattct
     3241 ccacgtgatg ggtgacctgc tgggctctgt cggcgccatc gttgcagcga tcgtcatcta
     3301 tttcaccggc tggacgccga tcgaccccat tctgtcggtc ttggtggcag cgttgattct
     3361 gcgcagcgcc tggaagctcc tggccaagtc gatccatatc ctgctggaag gcgcgccaga
     3421 ggatgcttcg ccggaaaagg tggaacaagg cctgatgagc actgtacccg gcctggcagc
     3481 tgtcagccac gttcatgtgt ggcaactcac ttccggtcgc acgatggcca cactgcacgt
     3541 tcgacccaat gtggacgagg aggcgcgagc cgtggtcaag cgcgtcgagg tggtgcttcg
     3601 agagcaattc agcatcgaac acgccacagt cggaatcgac tggaactcgg aggctgacga
     3661 gaacatctgc agcctgcaac ccacgacaga ccgtcaggac cacagcggcc atggccacag
     3721 tgagcacaat catgaccatg accactccgc tcctggtcac aagcattgag gccgtgcgag
     3781 ccggctcgca gttcacttga gggtttgaat ccatgacatc acgcaaaacc accagctccg
     3841 ctattgctca ctgttcgacc tccagccacg gcgcgaccac tgatgtcctc gcactgtcgc
     3901 aaagtgacgt cgctgttctg gccgagacct tccgcctgct gggcgaccaa tcgcgattga
     3961 aaatcctgtt gcagtgcatg cgcggatccg tcgcagtggg cgacattgcc gattcgctag
     4021 acctgtcgca atccttggtc agccatcacc tgcgcctgtt gcgcggcgcg cgcctcgtgc
     4081 gcggcgagcg ccaggccaag cacatcttct acggcatcgc cgaccagcac gtcagccagg
     4141 tactgcagga catggcggtt cacatttcgg aggacagaaa cgacgattga ctgaacgtgt
     4201 gagtgcgcgc cggcctactc cttcaagggg taggcttttt tttggctatc aatctagaga
     4261 aacaccgacg cgccttgctg aatgcctaga accccacata gcccggcaat tcctttccct
     4321 gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga tagagaaggg
     4381 tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg gccgtcctgg
     4441 ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca gtctatcgct
     4501 tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca ttgccagcca
     4561 gtgcccgtca ctcccgtact aactgtcacg aacccctgca ataactgtca cgcccccctg
     4621 caataactgt cacgaacccc tgcaataact gtcacgcccc caaacctgca aacccagcag
     4681 gggcgggggc tggcggggtg ttggaaaaat ccatccatga ttatctaaga ataatccact
     4741 aggcgcggtt atcagcgccc ttgtggggcg ctgctgccct tgcccaatat gcccggccag
     4801 aggccggata gctggtctat tcgctgcgct aggctacaca ccgccccacc gctgcgcggc
     4861 agggggaaag gcgggcaaag cccgctaaac cccacaccaa accccgcaga aatacgctgg
     4921 agcgctttta gccgctttag cggcctttcc ccctacccga agggtggggg cgcgtgtgca
     4981 gccccgcagg gcctgtctcg gtcgatcatt cagcccggct catccttctg gcgtggcggc
     5041 agaccgaaca aggcgcggtc gtggtcgcgt tcaaggtacg catccattgc cgccatgagc
     5101 cgatcctccg gccactcgct gctgttcacc ttggccaaaa tcatggcccc caccagcacc
     5161 ttgcgccttg tttcgttctt gcgctcttgc tgctgttccc ttgcccgcac ccgctgaatt
     5221 tcggcattga ttcgcgctcg ttgttcttcg agcttggcca gccgatccgc cgccttgttg
     5281 ctccccttaa ccatcttgac accccattgt taatgtgctg tctcgtaggc tatcatggag
     5341 gcacagcggc ggcaatcccg accctacttt gtaggggagg gcgcacttac cggtttctct
     5401 tcgagaaact ggcctaacgg ccacccttcg ggcggtgcgc tctccgaggg ccattgcatg
     5461 gagccgaaaa gcaaaagcaa cagcgaggca gcatggcgat ttatcacctt acggcgaaaa
     5521 ccggcagcag gtcgggcggc caatcggcca gggccaaggc cgactacatc cagcgcgaag
     5581 gcaagtatgc ccgcgacatg gatgaagtct tgcacgccga atccgggcac atgccggagt
     5641 tcgtcgagcg gcccgccgac tactgggatg ctgccgacct gtatgaacgc gccaatgggc
     5701 ggctgttcaa ggaggtcgaa tttgccctgc cggtcgagct gaccctcgac cagcagaagg
     5761 cgctggcgtc cgagttcgcc cagcacctga ccggtgccga gcgcctgccg tatacgctgg
     5821 ccatccatgc cggtggcggc gagaacccgc actgccacct gatgatctcc gagcggatca
     5881 atgacggcat cgagcggccc gccgctcagt ggttcaagcg gtacaacggc aagaccccgg
     5941 agaagggcgg ggcacagaag accgaagcgc tcaagcccaa ggcatggctt gagcagaccc
     6001 gcgaggcatg ggccgaccat gccaaccggg cattagagcg ggctggccac gacgcccgca
     6061 ttgaccacag aacacttgag gcgcagggca tcgagcgcct gcccggtgtt cacctggggc
     6121 cgaacgtggt ggagatggaa ggccggggca tccgcaccga ccgggcagac gtggccctga
     6181 acatcgacac cgccaacgcc cagatcatcg acttacagga ataccgggag gcaatagacc
     6241 atgaacgcaa tcgacagagt gaagaaatcc agaggcatca acgagttagc ggagcagatc
     6301 gaaccgctgg cccagagcat ggcgacactg gccgacgaag cccggcaggt catgagccag
     6361 acccagcagg ccagcgaggc gcaggcggcg gagtggctga aagcccagcg ccagacaggg
     6421 gcggcatggg tggagctggc caaagagttg cgggaggtag ccgccgaggt gagcagcgcc
     6481 gcgcagagcg cccggagcgc gtcgcggggg tggcactgga agctatggct aaccgtgatg
     6541 ctggcttcca tgatgcctac ggtggtgctg ctgatcgcat cgttgctctt gctcgacctg
     6601 acgccactga caaccgagga cggctcgatc tggctgcgct tggtggcccg atgaagaacg
     6661 acaggacttt gcaggccata ggccgacagc tcaaggccat gggctgtgag cgcttcgata
     6721 tcggcgtcag ggacgccacc accggccaga tgatgaaccg ggaatggtca gccgccgaag
     6781 tgctccagaa cacgccatgg ctcaagcgga tgaatgccca gggcaatgac gtgtatatca
     6841 ggcccgccga gcaggagcgg catggtctgg tgctggtgga cgacctcagc gagtttgacc
     6901 tggatgacat gaaagccgag ggccgggagc ctgccctggt agtggaaacc agcccgaaga
     6961 actatcaggc atgggtcaag gtggccgacg ccgcaggcgg tgaacttcgg gggcagattg
     7021 cccggacgct ggccagcgag tacgacgccg acccggccag cgccgacagc cgccactatg
     7081 gccgcttggc gggcttcacc aaccgcaagg acaagcacac cacccgcgcc ggttatcagc
     7141 cgtgggtgct gctgcgtgaa tccaagggca agaccgccac cgctggcccg gcgctggtgc
     7201 agcaggctgg ccagcagatc gagcaggccc agcggcagca ggagaaggcc cgcaggctgg
     7261 ccagcctcga actgcccgag cggcagctta gccgccaccg gcgcacggcg ctggacgagt
     7321 accgcagcga gatggccggg ctggtcaagc gcttcggtga tgacctcagc aagtgcgact
     7381 ttatcgccgc gcagaagctg gccagccggg gccgcagtgc cgaggaaatc ggcaaggcca
     7441 tggccgaggc cagcccagcg ctggcagagc gcaagcccgg ccacgaagcg gattacatcg
     7501 agcgcaccgt cagcaaggtc atgggtctgc ccagcgtcca gcttgcgcgg gccgagctgg
     7561 cacgggcacc ggcaccccgc cagcgaggca tggacagggg cgggccagat ttcagcatgt
     7621 agtgcttgcg ttggtactca cgcctgttat actatgagta ctcacgcaca gaagggggtt
     7681 ttatggaata cgaaaaaagc gcttcagggt cggtctacct gatcaaaagt gacaagggct
     7741 attggttgcc cggtggcttt ggttatacgt caaacaaggc cgaggctggc cgcttttcag
     7801 tcgctgatat ggccagcctt aaccttgacg gctgcacctt gtccttgttc cgcgaagaca
     7861 agcctttcgg ccccggcaag tttctcggtg actgatatga aagaccaaaa ggacaagcag
     7921 accggcgacc tgctggccag ccctgacgct gtacgccaag cgcgatatgc cgagcgcatg
     7981 aaggccaaag ggatgcgtca gcgcaagttc tggctgaccg acgacgaata cgaggcgctg
     8041 cgcgagtgcc tggaagaact cagagcggcg cagggcgggg gtagtgaccc cgccagcgcc
     8101 taaccaccaa ctgcctgcaa aggaggcaat ca
//
