LOCUS       NZ_CP119550             3123 bp    DNA     circular CON 11-APR-2024
DEFINITION  Providencia stuartii strain CAVP490 plasmid pCAVP490-3123, complete
            sequence.
ACCESSION   NZ_CP119550
VERSION     NZ_CP119550.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33687285
            Assembly: GCF_029277985.1
KEYWORDS    RefSeq.
SOURCE      Providencia stuartii
  ORGANISM  Providencia stuartii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Providencia.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Patel,A., Salomon,M., Barry,K., Parikh,H.I., Elliot,Z.S.,
            Fleece,M.E. and Mathers,A.J.
  TITLE     Management of PER-1 and NDM-producing Providencia stuartii
            bacteremia in a patient with a severe beta-lactam allergy: the
            increasing need for aztreonam-avibactam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Patel,A., Salomon,M., Barry,K., Parikh,H.I., Elliot,Z.S.,
            Fleece,M.E. and Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-2023) School of Medicine, University of Virginia,
            409 Lane Road, Charlottesville, VA 22908, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology :: Illumina MiSeq + Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029277985.1-RS_2024_03_11
            Annotation Date                   :: 03/11/2024 12:53:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,279
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 4,092
            CDSs (with protein)               :: 4,092
            Genes (RNA)                       :: 107
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 57 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 19 of 80
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119550.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Providencia stuartii"
                     /mol_type="genomic DNA"
                     /strain="CAVP490"
                     /isolation_source="human"
                     /host="Homo sapiens"
                     /db_xref="taxon:588"
                     /plasmid="pCAVP490-3123"
                     /geo_loc_name="USA: Virginia"
                     /collected_by="University of Virginia Health System"
     gene            complement(join(2609..3123,1..472))
                     /gene="mobV"
                     /locus_tag="P2E05_RS21360"
                     /old_locus_tag="P2E05_21360"
     CDS             complement(join(2609..3123,1..472))
                     /gene="mobV"
                     /locus_tag="P2E05_RS21360"
                     /old_locus_tag="P2E05_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_276123118.1"
                     /translation="MAFAIMRAKKLNSMGTVAAALQHCYRDRETPNADQERTPDNVHLA
                     ARSTDEAMGQLRERLPEKRRKDAVLAVEYVMSASPEWWQTASADQQREFFKRSTEWLAD
                     KYGAENVVTATIHRDELTPHLSAFVVPRTADGRLSAKEFIGNRTKMSQDQTSYAERVAD
                     LGLERGIEGSRATHQRVKTHYGAIQQAGRDVPHLTPDELKPQKVKGVSLAEKVFGAVET
                     VEGVAQRLNAKIMGSVQPMAEKAAVSAQNERRAKELRETLAQQQKRLQALQEPFKGLSK
                     DQVAGLIRQAVKLRQENEQEKQERAQQIKERFKAKRERERSDRSRGR"
                     /name="mobV"
     gene            1414..1962
                     /locus_tag="P2E05_RS21365"
                     /old_locus_tag="P2E05_21365"
     CDS             1414..1962
                     /locus_tag="P2E05_RS21365"
                     /old_locus_tag="P2E05_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_257766363.1"
                     /translation="MQSVTVATETQRRIRQRELAAADEKEAQEREEARKNKGFTQVYSK
                     GWRRVRELSKGNAGAAGLYAFFAEHIDPTCGAVVCDQQFLADHLEVSTRTIRNWLKYLE
                     TERALVRIPVAGRVCAYALDPHEVWKGYDNAKEYAAFVTKTLVNKDGDIKRRIMSMFSP
                     EERPDDRDTDTLDMFGDQP"
                     /name="helix-turn-helix domain-containing protein"
     gene            1959..2162
                     /locus_tag="P2E05_RS21890"
                     /old_locus_tag="P2E05_21370"
     CDS             1959..2162
                     /locus_tag="P2E05_RS21890"
                     /old_locus_tag="P2E05_21370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000131876.1"
                     /translation="MTDPLKALFGKPDYSHIVRDTTATISITAAEMAAVLEAYDRGIDT
                     LDGTTRTALDSVISKLKDEVWP"
                     /name="hypothetical protein"
     gene            2153..2374
                     /locus_tag="P2E05_RS21895"
                     /old_locus_tag="P2E05_21375"
     CDS             2153..2374
                     /locus_tag="P2E05_RS21895"
                     /old_locus_tag="P2E05_21375"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001251694.1"
                     /translation="MAMSMYYIKTGLEYWKPKGYGYTKSREEAGVFTLKDMEELNLDGC
                     TLERAEVAFLDEDGPSPDLPHRVTLPRD"
                     /name="hypothetical protein"
     gene            2457..2612
                     /locus_tag="P2E05_RS21380"
                     /old_locus_tag="P2E05_21380"
     CDS             2457..2612
                     /locus_tag="P2E05_RS21380"
                     /old_locus_tag="P2E05_21380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001736727.1"
                     /translation="MPVNPRDYCQPLHPGCLGLVESGLGLVESGLMLVKQWFKVVQGWL
                     KLVEKG"
                     /name="hypothetical protein"
ORIGIN
        1 cgtagctggt ctggtcctgg ctcatcttcg tccggttgcc aatgaactcc ttggcactca
       61 ggcgcccgtc agccgtccga ggcaccacga acgccgacag gtgcggggtc agctcatccc
      121 ggtggatcgt ggccgtgacc acgttctcag cgccgtactt gtcggccagc cactcggtcg
      181 atcgcttgaa gaactcccgc tgctggtcgg cgcttgcggt ctgccaccac tccggcgagg
      241 cgctcatcac gtattcgacc gccagcaccg catccttgcg gcgcttctcc ggcagcctct
      301 ccctgagctg gcccatcgcc tcatcggtcg atctggccgc caggtgaacg ttgtccggtg
      361 tgcgctcctg atcggcgttc ggcgtctcgc ggtcgcggta gcagtgctgc aacgcagccg
      421 ccaccgtgcc catgctgttg agcttcttcg ccctcatgat cgcaaacgcc atccgtcccc
      481 ctccgcactg tcagattgct gccagcccgt cgaggtacga atgtgcctcc ggcgggccac
      541 ttctttgaag tgtactcact agactttact tcgtaaagta cgtgaccgct acgcggttgc
      601 agcggcctgc ggccttgctc tacgggcttc gccctgcgcc tcccttcggt cgttgcgaac
      661 accttggggc gctgccccaa accccgcgtt cgccacgtgg caggggtgca ggtggccgcg
      721 ccgcttcgct ctgctctacc tgcccccttg cctgagcgtg ggggtcactc catcacgtca
      781 agggttcgct tcgccgggtg ctcacccgat cccttccgct tcgctccagg gctgcggctt
      841 ccgctcccgc ccttgacctg attccgttcc gagtggaggc agcgtcaagg ggtcgtggaa
      901 taccgcaagg cgcgcagcgc ctgaggatat gccgcgaaag ccccttgacg ctgccggaac
      961 gacccagcct ctggctctgg ggttgggaac gaagggagcg cagcgaccgc aggccccagc
     1021 ccccgagcct cacggcggcg agtgtgggga gtccagaggg gcaaagcccc tttgggccta
     1081 gcgcagcgga aacaaaccct ctcgcctctg gcgaggcatt aactccaagg gagcgcagcg
     1141 accgtcagca gggtgccaag cgaagcgccg gcgggacggc agtcccttag aggagaaggg
     1201 gaaaaatact aaatgattga tttatatagt ttttaaattt tgtgatagga aacgtagggt
     1261 tccggacaac cggaagcagc ttgtttccgc ttacttccta tcataggaaa caaattgctt
     1321 gacttgggaa acggttttgg gaaataattt tcctatggta ggaattaaac gtccggttaa
     1381 gcggacgccc tgtttccggt taggaggtca acaatgcaaa gcgtcaccgt agcgacagag
     1441 acacagagac gaatcaggca aagggagctg gcagcagcag acgagaagga ggctcaggag
     1501 cgcgaggagg cgcgaaagaa caaggggttc acacaggtat attcaaaggg atggcggcgc
     1561 gtcagggagc tgtcaaaggg caatgcgggg gccgctggcc tgtatgcctt cttcgctgaa
     1621 cacatcgacc ctacctgtgg cgctgttgtc tgcgatcagc agttcctggc tgaccatctg
     1681 gaggttagca cccgcacgat tcggaactgg ctcaagtacc ttgagacaga acgcgccttg
     1741 gtcagaatcc cagtcgctgg gcgggtttgc gcctacgctc ttgaccccca cgaggtctgg
     1801 aaagggtacg acaacgccaa agaatacgcg gccttcgtga ccaagacgct ggtcaacaag
     1861 gacggcgata ttaagcgccg gatcatgtcg atgtttagcc cagaggaacg cccagacgac
     1921 agggatacag acacgcttga tatgtttgga gatcagccat gacagatcca ctcaaagccc
     1981 ttttcggcaa gcctgattac tcccatatcg taagggacac cacggccact atcagcatca
     2041 cagccgctga aatggccgct gttttggagg cttacgacag gggtatagac acgctggacg
     2101 gcaccaccag aacggcgctg gattccgtta tatcgaaact caaggacgag gtatggccat
     2161 gagcatgtat tacatcaaga caggtctgga gtactggaaa cccaagggct acgggtacac
     2221 caagagccgc gaggaggcgg gggttttcac cctcaaggac atggaagaat tgaacctcga
     2281 tggctgcacc ctggaacgcg ccgaagtggc gttcctggac gaggacggcc ccagtcccga
     2341 cctaccccac cgcgtcaccc tgccccgcga ttgagcgggg cgctggaggg tttcaaaggg
     2401 gcaccctttg gcacgggata tgtgtcaaaa acggagtttt tgtaaacata tccgacgtgc
     2461 ctgtaaatcc gcgcgattac tgccaaccct tacaccccgg ttgcttaggg ttggttgaaa
     2521 gtggtttagg gttggttgaa agtggtttaa tgttggttaa acaatggttt aaggttgttc
     2581 aaggttggtt aaagttggtt gaaaagggtt aacggcccct gctgcggtcg ctgcgctccc
     2641 gttcccgctt ggccttgaat cgctctttga tctgctgggc gcgctcctgc ttctcctgct
     2701 cgttctcctg ccgcaatttc accgcctgcc ggatcagtcc cgctacctga tccttggata
     2761 gccccttaaa cggctcctga agggcctgta agcgtttttg ctgctgggct agggtttccc
     2821 ttagctcctt ggctcttcgc tcgttctggg cgcttacagc ggctttctcg gccatcggct
     2881 gcacagaccc cattattttg gcgttcaacc tttgcgccac cccttcgacc gtctccacgg
     2941 ctccgaacac cttctcggcc agtgacaccc ccttgacctt ctggggtttc agctcgtcag
     3001 gcgtcaggtg tggcacgtct cggcctgcct gctggatcgc cccatagtgg gttttaactc
     3061 gctggtgtgt ggccctgctg ccctcgatgc cccgttcaag ccccagatcg gccacccgtt
     3121 cgg
//
