LOCUS       NZ_CP119176             5596 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Klebsiella pneumoniae strain X-CRHVKP-130 plasmid p2, complete
            sequence.
ACCESSION   NZ_CP119176
VERSION     NZ_CP119176.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33555564
            Assembly: GCF_029167725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Liu,Y.
  TITLE     Genome analysis of a novel carbapenem-resistant hypervirulent
            Klebsiella pneumoniae strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2023) Department of Clinical Laboratory, Medical
            Center of Burn Plastic and Wound Repair, The First Affiliated
            Hospital of Nanchang University, Yong Wai Zheng Jie No. 17, Nanchang
            330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 25.3x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029167725.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 06:45:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,879
            CDSs (total)                      :: 5,757
            Genes (coding)                    :: 5,560
            CDSs (with protein)               :: 5,560
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 67 of 197
            Pseudo Genes (incomplete)         :: 138 of 197
            Pseudo Genes (internal stop)      :: 25 of 197
            Pseudo Genes (multiple problems)  :: 33 of 197
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119176.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="X-CRHVKP-130"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2"
                     /geo_loc_name="China:Nanchang"
                     /collection_date="2018-10"
     gene            complement(join(5447..5596,1..396))
                     /locus_tag="PY995_RS26595"
                     /old_locus_tag="PY995_26595"
     CDS             complement(join(5447..5596,1..396))
                     /locus_tag="PY995_RS26595"
                     /old_locus_tag="PY995_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(422..748)
                     /locus_tag="PY995_RS26600"
                     /old_locus_tag="PY995_26600"
     CDS             complement(422..748)
                     /locus_tag="PY995_RS26600"
                     /old_locus_tag="PY995_26600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1106..1264)
                     /locus_tag="PY995_RS29400"
     CDS             complement(1106..1264)
                     /locus_tag="PY995_RS29400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1904..2080
                     /locus_tag="PY995_RS26605"
                     /old_locus_tag="PY995_26605"
     CDS             1904..2080
                     /locus_tag="PY995_RS26605"
                     /old_locus_tag="PY995_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2070..2414
                     /locus_tag="PY995_RS26610"
                     /old_locus_tag="PY995_26610"
     CDS             2070..2414
                     /locus_tag="PY995_RS26610"
                     /old_locus_tag="PY995_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2623..>3057
                     /locus_tag="PY995_RS26615"
                     /old_locus_tag="PY995_26615"
                     /pseudo=""
     CDS             2623..>3057
                     /locus_tag="PY995_RS26615"
                     /old_locus_tag="PY995_26615"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3092..3601
                     /locus_tag="PY995_RS26620"
                     /old_locus_tag="PY995_26620"
     CDS             3092..3601
                     /locus_tag="PY995_RS26620"
                     /old_locus_tag="PY995_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            3608..3820
                     /locus_tag="PY995_RS26625"
                     /old_locus_tag="PY995_26625"
     CDS             3608..3820
                     /locus_tag="PY995_RS26625"
                     /old_locus_tag="PY995_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4487..4711
                     /locus_tag="PY995_RS26630"
                     /old_locus_tag="PY995_26630"
     CDS             4487..4711
                     /locus_tag="PY995_RS26630"
                     /old_locus_tag="PY995_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4695..4961
                     /locus_tag="PY995_RS26635"
                     /old_locus_tag="PY995_26635"
     CDS             4695..4961
                     /locus_tag="PY995_RS26635"
                     /old_locus_tag="PY995_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5031..5381)
                     /locus_tag="PY995_RS26640"
                     /old_locus_tag="PY995_26640"
     CDS             complement(5031..5381)
                     /locus_tag="PY995_RS26640"
                     /old_locus_tag="PY995_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     Typing          1739..1818
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1156..1553)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2405..3936
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgagttaagg gtttctttta tactttcttc aatgctcatt ataaatatta gtggattatc
       61 atttttcttc ccccatgggt ttctaattaa tccttcgatg tactcaggtc ctttgcgtcc
      121 tattgcctgc gcaatatttt ttatttcatc tgcgtgaggg ctttgttgta cagctttgtt
      181 tatgcctgct tgagtgattt tcagagactc tacaccgtgt atgttgtatg agataaaatg
      241 taataaatta agatgcaatg caattaatcc atttttgttt acatctgagt atttggataa
      301 aaaacgtaat gaagtgttaa ctctcccttg aagcctaaca attgggagtg ctgccttgta
      361 tatttccttc tccgttaaat accgtttaaa aaacataatt ctccgcctcc gtatagcgtg
      421 cctattttat ttcgtcgtaa aacttagctc tgaaatattc ctttagaacc atcatgtcat
      481 taataattga atctatctca tccatttttt cttggtattc ttttaatggt gaagggagcg
      541 gtaactcaat atttgaaaag tgattggcga gatagggatc aaggtttctc atccattcag
      601 gcttttcgcc aatgcgttct tcaactgaaa gtgaacctga taatctctgg ttaataagat
      661 cacttagact cattttggtt ttattcgaaa gccgttgtaa ggattgcttt aatcctatat
      721 ctagcatgat atttgttctt tctctcatta cccccccttg ctattgcgtt ttttgttgcg
      781 cacaaagcta ccattgcgca ttaagaaacg caataaaaaa ggagggaggg agtggtttgt
      841 acatgatgtg cagtgcgctg agggcatcag tggcgggtgt ttttccgtgg gatggatgga
      901 gctccgggga taacagaact gacgatcatt taaaggatct tcttgagatc ctttttttct
      961 gcgcgtaatc tcttgcactg taaacgaaaa aaccgcctgg ggaggcggtt tgatcgaagg
     1021 ttaagtcagt tggggaactg cttaaccggg taactgggta tacgagaccg cagataccaa
     1081 aactgttctt tcagtgtagc cgcagttagg cacccacttc aagacctctc aatatctctc
     1141 tcgtatatat ccagttacca atggctgctg ccagtggcgt ttttgcgtgt ccgtccgggt
     1201 tggactcaag atgatagtta ccggaaaggg cgcagcagtc gggctgaacg gggggttcgt
     1261 gcatacagcc cagcttggag cgaacgacct aaaccgagcc gagataccag cagcgtgagc
     1321 tatgagaaag cgccacgctt cccgaaaggg aaaaaggcgg aacaggtatc cggtaaacgg
     1381 cagggtcgga acaggagagc gcacgaggga gccgccggag agaaatcacc ggcatcttta
     1441 agtcctgtcg ggtttcgtcc tcctctgact tgagcgtcca tttctgtgat gctcgtcagg
     1501 ggaggcggag cctatggaaa aacggcaacg ccgcatcctt ttctccggcc ctttctgatt
     1561 tgagcgcctt tgctttatcc cgttatccgt gagtcggtca cgcccgccgc agccgaacgg
     1621 caggagcgca gctaccgagt gagcgaggag gcgtcatttc atctgtccgt gcatggtatt
     1681 gcggacgctc ctgagccaca tatcacacct gccacatgag gcactttcat gaatcgtgct
     1741 ctcatgccaa catagtaagc cagtatacac agcgtaaacg ctgtgactgg ttcagggctg
     1801 cgccccgaaa cccgctaaca ccactgacgc gctggcgctt gtccaacgcc ggagcgaccc
     1861 ggaaaacttt tccgtgccgt ccggcgtttg cgaaggaggg ataatgcaga agccgaagaa
     1921 gaagccgctc gtcgtaaaaa aaagatcctg gcatccggca gtgatggcaa atctgatgcg
     1981 tcaggcacag gaggaagatt accgccggga gcagatggca gagcttgagc gttcagcccg
     2041 gtcacatttt gcggatatcg gaggagagga tggcggttaa gcgtacccgt tttttaggta
     2101 tccgggtgac cgatggggag taccagcagc tgctggagcg ctgcaatgga aggcaactgg
     2161 cggtgtggat gcgggaaacc tgtctcgata cgcgcccggc gcggtcgttg cggctgccgt
     2221 ccatcgatcc ggttttgctg cgccagcttg ccgggatggg gaacaacctc aaccagatcg
     2281 cccggaaaat taacggcggc cagtggtcag gcgctgaccg ggttcaggtg gtggccgcgc
     2341 tgatggccat tgatgccgaa ctcgagcggc tgcggcatgc cgtgctggaa aagggggcag
     2401 acgatgatcg ttaagtttca tccgcgaggg cgaggcggcg gtgccgggcc ggtggattat
     2461 ctgctgggga aagaccgcca gcgcgacggg gccatcgtcc tgcagggaaa gccggaggag
     2521 gtccgggagc ttatcgacgc ctcgccctac gccaaaaagt acacctccgg cgtcctgtct
     2581 tttgctgaac aggatttacc gcccggccag cgtgaaaagc tgatggcgag cttcgagcgg
     2641 gttctgatgc ccggactcga taaagaccag tacagcgtgc tgtgggttga acaccgggac
     2701 aaggggcggc tggaactgaa ttttctggta ccgaacacgg aactgctgac cggcaggcgt
     2761 ctccagccgt actacgaccg ggcagaccgt ccgcgcatcg atgcgtggca gaccgtggtg
     2821 aacgggcgac tggggctgca cgacccgaac gcaccggaga accggcgcgc gctggtgacg
     2881 ccatcttcgt tacccaaaac gaagcaggaa gccgcagagg ctattacgcg gggcttactg
     2941 gccctcgcct cgtcagggga gcttaaaacg cgtcaggacg tcactgaggc gctggaaagc
     3001 gcaggttttg aggtcgtgcg caccacaaag agcagcatca gcattgccga cccggacgga
     3061 gggcgaaaca tccgacttaa gggagccatc tatgaacagt cttttaacgc tggcgaagga
     3121 cttagagcag aaatcgaaag cgcagcagca gagtaccggc gaaatgctga aagccgcatt
     3181 cagcgagcac gagaagtctg tcagagcgga actgagcgaa agcgggaaga gaatcagcgc
     3241 cgccatccgc gcccacgagc agggtatgag cgcggccatg cagtcgaacc gcctgagcgt
     3301 gatgcgtatg gtcgggcgga catggctgac catcacgatg gtgtcaggac tgctgttcgc
     3361 cagcctgagc ggggtgttgt ggtatcaggg gagcttgata gcgtcaaatc tggccgaaat
     3421 cgaccggcag aacgcagcgc tgtcgaagct gaacgcgaag acctggggcg tgacgtacct
     3481 ggaagacagc aacggacgtt ttctggtgct gccgaagggg acggccgtag acaggacgca
     3541 gagctggacg gtcgggaacg ggcggtcgaa gcagaacgcg ctgagactgg tgaaggagta
     3601 agtgccgatg accgagctgg aaaaacagtt gctgagcgca ttagagcagc tacagcagga
     3661 ctactcgaaa aggctggacg agtgggagag cgccttcggg gaatggcgga aaatgtcagg
     3721 gcttatgcaa cgggagaacg cggcgctgag cgagcgcgtg acgcgcttga gtcagcaggt
     3781 ggaacgcttg agcgggcaac tgcagcgttt gagccggtag tgcagcgtca tgagatggcc
     3841 gtggccgcag aacgggcgca cgagcaacgg cagcacgaaa aggaactgac ggaagcgcgt
     3901 tcccggcagc gaagctatga cggtccgtcg ctgggctgaa atgcaggaaa acagggttct
     3961 catcgggcat ttacgctgaa accggatgtc attcacctgg ctgcatggct atgcagccag
     4021 gtaaaattta tctgtcgcga acggggcggt ttatcgggtg gtttgttgcc ggttttacct
     4081 gactgccacc ccgttcgcgg cgaacccgtc cggggcggtg cgggcaacgg gtgttatgtt
     4141 taatcagcgt gaacggcaga tacaaaaaag ccccggaggg cttttaacta ctgacttatt
     4201 tttttgaaga tttgctgggt ttcaatttgt cagtatttcc cggcgttttt ttattgtcac
     4261 gctggcgttt atcaggttcc cctgttgaat cggcaattcg tttcggaccc ggcttaagac
     4321 ccataatcgt tatcctttca atagttaaga ggaaagccct caagctagat atattgaccg
     4381 cgttacggat tatcaagagg ggctaacaag attatttttt gttcttttag gttgattgtg
     4441 gtactgttga tatacatgta tagcaaatgg tgttcgggag gtttatatgc ttgctatccg
     4501 gttatctgat gagattgagt cccgtctgga ctcgctggcg aagcaaaccg gcagaacaaa
     4561 gacgttttat gcgcgggaag caatactggc gcatctggaa gacctggagg attattatct
     4621 ttcagcagaa actgctgcac gcgttcgccg tggtgatgaa gcagtgcatt cgtctgaaga
     4681 cgtgaggaag tcacttggtc tggacgatta actattccga tcgggcgctc aaatcgttac
     4741 gcaagatgga caaacagaac gcacgacgga ttgtggattt tatgagttta cgcattgcag
     4801 ttgctgccga tcctcgccag tcagggaagc cgctcaaagg tgagctgggc gagttctggc
     4861 gctatcgggt gggagattat cgcgttctgt gtgagatccg agatgacgag cttgttatcc
     4921 ttgccgccac gattggacat cgccgcgaag tttacgactg aagcccgcta tgcgggcttt
     4981 tttgatgccg acaacaggta ttatggtaaa tactcgacta actacgtgtt ctactgtttg
     5041 tcggttttag ttaaccagca ggttgtctta acgataatgt agccgttatt tttaactaat
     5101 aaggtcatat tgttaacgtc aggcataata aaagacatta tggcctcaga gttatcatca
     5161 gaagcagctc tctgcattac tacttcgatt cccttttctt tgaatctata agtatccagt
     5221 tgcctattaa acttagaaag acctacaacc atacttagtt tgttatcttt aacagtaaga
     5281 gatgcttcct gagtaccatt acatcggtag taagcggtgt aaccgtcagc aaaagatcga
     5341 ccggggaata taaatgctag gaacgcaatt aaccaataca tagttgagaa tcttggtatt
     5401 acacatttca ctatcctctc tttcattttt cgttcaccat actttgttat ctgattttta
     5461 atttttctat tttataaacc aagtctctac caaaaaggcg aaaattatcc tcaaataata
     5521 ataattgttt aagaacatcc atgaaagcag ctgcttcacc ctcagttatg ggagctactc
     5581 tatataacat ataacc
//
