LOCUS       NZ_CP119175            11970 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Klebsiella pneumoniae strain X-CRHVKP-130 plasmid p1, complete
            sequence.
ACCESSION   NZ_CP119175
VERSION     NZ_CP119175.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33555564
            Assembly: GCF_029167725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Liu,Y.
  TITLE     Genome analysis of a novel carbapenem-resistant hypervirulent
            Klebsiella pneumoniae strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2023) Department of Clinical Laboratory, Medical
            Center of Burn Plastic and Wound Repair, The First Affiliated
            Hospital of Nanchang University, Yong Wai Zheng Jie No. 17, Nanchang
            330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 25.3x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029167725.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 06:45:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,879
            CDSs (total)                      :: 5,757
            Genes (coding)                    :: 5,560
            CDSs (with protein)               :: 5,560
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 67 of 197
            Pseudo Genes (incomplete)         :: 138 of 197
            Pseudo Genes (internal stop)      :: 25 of 197
            Pseudo Genes (multiple problems)  :: 33 of 197
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119175.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="X-CRHVKP-130"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1"
                     /geo_loc_name="China:Nanchang"
                     /collection_date="2018-10"
     gene            370..1857
                     /gene="ltrA"
                     /locus_tag="PY995_RS26525"
                     /old_locus_tag="PY995_26525"
     CDS             370..1857
                     /gene="ltrA"
                     /locus_tag="PY995_RS26525"
                     /old_locus_tag="PY995_26525"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            1962..3647
                     /locus_tag="PY995_RS26530"
                     /old_locus_tag="PY995_26530"
     CDS             1962..3647
                     /locus_tag="PY995_RS26530"
                     /old_locus_tag="PY995_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3657..3914
                     /locus_tag="PY995_RS26535"
                     /old_locus_tag="PY995_26535"
     CDS             3657..3914
                     /locus_tag="PY995_RS26535"
                     /old_locus_tag="PY995_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            3999..4148
                     /locus_tag="PY995_RS26540"
                     /old_locus_tag="PY995_26540"
     CDS             3999..4148
                     /locus_tag="PY995_RS26540"
                     /old_locus_tag="PY995_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            5632..7596
                     /locus_tag="PY995_RS26545"
                     /old_locus_tag="PY995_26545"
     CDS             5632..7596
                     /locus_tag="PY995_RS26545"
                     /old_locus_tag="PY995_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            7596..8327
                     /gene="mobC"
                     /locus_tag="PY995_RS26550"
                     /old_locus_tag="PY995_26550"
     CDS             7596..8327
                     /gene="mobC"
                     /locus_tag="PY995_RS26550"
                     /old_locus_tag="PY995_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            8334..8864
                     /locus_tag="PY995_RS26555"
                     /old_locus_tag="PY995_26555"
     CDS             8334..8864
                     /locus_tag="PY995_RS26555"
                     /old_locus_tag="PY995_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8891..9070)
                     /locus_tag="PY995_RS26560"
                     /old_locus_tag="PY995_26560"
     CDS             complement(8891..9070)
                     /locus_tag="PY995_RS26560"
                     /old_locus_tag="PY995_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(9096..9524)
                     /locus_tag="PY995_RS26565"
                     /old_locus_tag="PY995_26565"
     CDS             complement(9096..9524)
                     /locus_tag="PY995_RS26565"
                     /old_locus_tag="PY995_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(9688..10002)
                     /locus_tag="PY995_RS26570"
                     /old_locus_tag="PY995_26570"
     CDS             complement(9688..10002)
                     /locus_tag="PY995_RS26570"
                     /old_locus_tag="PY995_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9990..10268)
                     /locus_tag="PY995_RS26575"
                     /old_locus_tag="PY995_26575"
     CDS             complement(9990..10268)
                     /locus_tag="PY995_RS26575"
                     /old_locus_tag="PY995_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(10443..10808)
                     /locus_tag="PY995_RS26580"
                     /old_locus_tag="PY995_26580"
     CDS             complement(10443..10808)
                     /locus_tag="PY995_RS26580"
                     /old_locus_tag="PY995_26580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(10805..11176)
                     /gene="tolA"
                     /locus_tag="PY995_RS26585"
                     /old_locus_tag="PY995_26585"
     CDS             complement(10805..11176)
                     /gene="tolA"
                     /locus_tag="PY995_RS26585"
                     /old_locus_tag="PY995_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(11450..11695)
                     /locus_tag="PY995_RS26590"
                     /old_locus_tag="PY995_26590"
     CDS             complement(11450..11695)
                     /locus_tag="PY995_RS26590"
                     /old_locus_tag="PY995_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..8647
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4252..4830)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7597..8324
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 acagtgtgcg ccgggagacc ggttagagat caaggggtga aagtccccga ccattgaagg
       61 accagcaatc cacaaggtcc ccgagtcatg cgttgcatac cgcgaggtat ggggcgaagc
      121 gttgacaggg gtgttgacag gccagccatt gagccacgaa atgtatatta aattaccggg
      181 tgccgacgtt gtactgttaa cggaaggcaa catcataggg tgcgacactg cgagtgccac
      241 atggacccgg cggggtctga gaccctggca tgtcaatacg atctctacgc gggaaccggg
      301 agatctcccc tctgaccatc tgccagtgtc ggagatggcc cgcaccggga agacgaggag
      361 tcatagccgg tgatgtacgg agaggagaag tcggactcgc tcatagtagc ggcgaagcag
      421 gcgaacaacc cgaaaggagc ggagtcagtg gagcgaagga gcggggccaa ggggaacgcg
      481 gaacagccac acatgcgccg gacacagagc cgggaaagca tgtcacagag gctgtcacgc
      541 gtgcgggaag ctgcgaagca gcggaagaaa gaacggttta cagcattgtt ccacctgctg
      601 acagtcgaag cactggaagc cgcattcctc tccctgagca ggaaagcggc cgccggagtg
      661 gatggcatca ggtggatgga ctacgccgga aacatgaaga acaacataac agatctgcac
      721 cggaggctac atcagggcag ctacagggcg cagcccggca ggcgtcacta catcccaaaa
      781 gcggatggaa aacaacgccc gctcggcatc gcctcgctgg aggacaagat cgtccagtat
      841 gcgctggtga aaatcctgaa cgcagtctat gaaaacgact ttatggggtt ctcatacggg
      901 ttcagacccg ggcgaagcca gcacgatgca ctggacgcac tggccacagg gctggtacgc
      961 actaacgtaa actgggtact ggatgccgac atcagtcagt tcttcgacag ggtgagccac
     1021 gaatggctga tcaggttcac agagcatcgg atcggcgacc ggagggtaat caggctcata
     1081 cgtaagtggc tcacagccgg gacgtcggag gagggtcaat ggcgagcaac ggaggaaggc
     1141 accccacagg gtgcggtcat ctcaccgctg ctggcaaaca tatacctcca ctacgtcttc
     1201 gatctgtggg cgcatcagtg gcgacgtcgc tatgccacag gcaatgtggt aatggtcaga
     1261 tacgccgatg acatcgtcat cgggttcgac aaacgatacg atgcccggcg cttccgtata
     1321 gccatgcagc gcagactgag ggagttcgga ctcacggttc acccggagaa aacccgtctg
     1381 atggagttcg gccgcttcgc tgccgaaaac cgtgccatca ggggaaaagg caaaccagaa
     1441 acgttcaact tcctcgggtt cacgcacatc agcgggaaag atcgcaacgg caggttcatg
     1501 ctgatacgaa agacccgccg ggatcggatg acggcaactc tgaaagccat caaagacggt
     1561 ctgcgaaggc gctggcatta ctcaatcccc gaacagggaa aatggctcag gagagtggtt
     1621 cagggatacc tgaactatca ctcggtaccg ggcaacttcc ccaccatgca gaagttcagg
     1681 acacacgtaa caaacctctg gcgccgggcg ctcaggcgca ggagccagaa ggatgatacg
     1741 acctggacga aagcaaacaa actggcagcc gcatggctac caagggttcg ggttcttcat
     1801 ccatggcctg tggagcggtt caccgccaga cacccgaggc aggagcccgg tgcgtaaatc
     1861 gcgcacgccg ggatctgtgc ggggggtatc cggtaacggg tatccctacc gcgacatgtt
     1921 ggctttaatt tattgtttta aatgtcaaag aggaaaacgg tatgagtggt ggagacggtc
     1981 gaggtccggg taattcaggt ctgggacata atggtggtca ggccagtggg aatgtgaacg
     2041 gtacgtctgg taaaggtggc ccttcatcag gtggtggtac ggatccaaac agcgggccgg
     2101 gctggggtac gacgcatacg ccgaatggag atattcataa ctataatccg ggggagtttg
     2161 gtaacggcgg gagtaaaccc ggtggtaatg gtggtaacag cggcaatcat agcggtagct
     2221 ctggtggcgg acagtcttcg gccactgcga tggccttcgg tctgcctgcc ctggctactc
     2281 cgggggctga aggactggct ttatcagttt ccggcgatgc gttgtcggcc gccgttgctg
     2341 atgtgctggc tgccctgaag gggccgttta agtttggtct gtgggggatt gcgatctacg
     2401 gtgtgctgcc ttctgagatt gcaaaagatg atccgaaaat gatgtcaaaa attatgacgt
     2461 cattaccggc cgatgcggtg acggagactc cggcaagtac tttaccactg gaccaggcga
     2521 cggttcgtgt cagacaacgg gttgtggatg tggtgaagga tgagcggcag catattgcgg
     2581 ttgtcgcagg tcggccaatg agtgtccctg tggtggatgc gaaaccgaca aaacgtccgg
     2641 gggtattcag tgtgtcgatt ccgggtctcc cgtctctgca ggtgagcgta ccgaaaggtg
     2701 ttccgacagc gaaagccccg ccaaaaggca ttgttgctga aaaaggtgat tcacgtccgg
     2761 ctggttttac agccggtggt aactcccgtg aggccgttat tcgtttcccg aaagagaccg
     2821 gacagaagcc ggtttatgtg tcggtgacag atgttcttac cccggcacag gtaaaacagc
     2881 gtcaggagga agaaaagcgt cgccagcagg catgggacgc cgctcatccg gaagaggggc
     2941 tgaaaagaga ctatgataaa gcgaaagctg agctggatgc cgaagataaa aatattgcga
     3001 ccttaaacag ccgcattgca tcgacagaga aggcgctccc cggtgcaagg gctgctgttc
     3061 aggaagccga taaaaaggta aaagaggcag aggcaaataa agatgatttt gtgacttata
     3121 acccgcctca tgaatatggc tccgggtggc aggatcaggt tcgctatctt gataaggata
     3181 ttcagaatca gaatgagaaa ttaaaagcgg ctcaggcatc tttaaacgca atgaatgaat
     3241 ccttatccag agataaggct gcacttaccg gggcgatgga gagccggaaa caaaaggaga
     3301 aaaaagcgaa ggatgcagaa aataagttaa atgaggaaaa gaacaagcct cgcaagggga
     3361 ctaaagacta cggccatgat tatcatccag tacctaaaac agaagacata aagggacttg
     3421 gagagttaaa gcgaggtgat cctaaaacac ctaaacaagg tggtggtggt aagcgggccc
     3481 gatggtatgg cgataaaaaa cgtaaaattt atgaatggga ttcccagcac ggtgagcttg
     3541 aagggtatcg tgccagtgat ggcgaacacc taggcgcatt tgatcccaaa acgggtaagc
     3601 aggttaaagg gccagatcca aaacggaaca ttaaaaaata tctttgagag gtaaatatgg
     3661 ggcttaaatt acgtttgaca tggtttgata agaaaactga agagtttaaa ggggaagagt
     3721 attctaaaga ctttggtgac gatggttctg tcattgaaag cttgggaatg cctttaaagg
     3781 ataatattaa taatggtgag tttgatttga agaaagaatg ggttccttta ctacagccat
     3841 tttttaaaaa tcaaataaaa cctgatgtgg ataattgtta tatagctttt gattatcggg
     3901 acggtaactg gtaacaaaaa attatcaagg atgatttgaa atactgactt ccggtgttca
     3961 gggatgaatg ccggagtctt taaatacagg gaaagtatat gaaaaaggta aagactattt
     4021 ttctttttat attgattgtt gctggctttc ttctggccgc atgtcaggca aattatatcc
     4081 gggatgttca gggcggaacg gtggcaccat cgtcctcctc tgaactgacg gggatcgcgg
     4141 ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatcttttg
     4201 ccctgtaaac gaaaaaacca cctggggagg tggtttgatc gaaggttaag tcagttgggg
     4261 aactgcttaa ccgtggtaac tggctttcgc agagcacagc aaccaaatct gtccttccag
     4321 tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa acaaatcctc
     4381 tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt ttgcgtgctt tcccgggttg
     4441 gactcaagtg aacagttacc ggataaggcg cagcagtcgg gctgaacggg gagttcttgc
     4501 ttacagccca gcttggagcg aacgacctac accgagccga gataccagtg tgtgagctat
     4561 gagaaagcgc cacacttccc gcaagggaga acggcggaca ggtatccggt aaacggcagg
     4621 gtcggaacag gagagcgcaa gagggagcga cctaccggaa acggtgggga tctttaagtc
     4681 ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc ctcacggctc ccacagatgc
     4741 accgaaaaag cgtctgttta tgtgaactct ggcaggaggg cggagcctat ggaaaaacgc
     4801 caccggcgcg gccctgccgt tttgcctcac atgttagtcc cctgcttatc cacggaatct
     4861 gtgggtaact ttgtatgtgt ccgcagcgcc cgccgcagtc tcacgcccgg agcgtagcga
     4921 ccgagtgagc gaggaggcgc aatttccccg gcacctgcaa gacggtcacc ggacgcgctg
     4981 atgtgggata ttgtcccctg attaagtctt tctattccct gacgatcaat tatcaggata
     5041 ggtgaaccat gtcggttttt cgctgaacat tttacgttaa cctatctctg ttgctttttt
     5101 ctccttgcgg agaaatgcaa gtcatccccg ctggggcgtg gctgacttca ggggaaaaga
     5161 ccttgcttac gctggctgga cgccaccgac gcacggtctt ttcccctctt tctggctctg
     5221 tccggctgga cgccggccac gccagaaatt cacccggccc ccgtcaggct cacgcctgac
     5281 cacccacaaa aatccacggt gctggacgca cctgacgaag cagcaaagct gctcctgatt
     5341 ttgcgggtgg tcagccgctc gctatggcga actctgacgg gggccggaac accggaaaag
     5401 accgcactgg ggtgccgcct tttcctctca cacgctgggg cgtgctcgtt gttcctccgg
     5461 ggctgcgcct tttccgcatg gggcggaaag aggctggcct ccgcggtgaa agctgaacgc
     5521 aatcaccacg gacgtgaccg ccgccgccat gccttggggc atgtccgcgg cttgtgcagc
     5581 gggggctgcg catcacgccg gtaaacccgt cgtctgaagg agagagattg catgtttaat
     5641 acggattcgc ttgcctggca gtggcatatc ccggagctgg cccggcagat ttacccgtgg
     5701 gtcagctggc tgtaccggga gtattacagt ctggccggtt tttttctggt tatcggcctg
     5761 tttttcggtc tggctgaact ctgggccggc cgaatgcgtt ttgtggggct gagccataag
     5821 gcgcggcggg agtatgccga tcgctataag cgcgataata accggactgt gccattttcg
     5881 gtgctggcct cgctggattc acccgggcgg atggtcaggg cacggcgaca tgtctggaac
     5941 tacgcgtact ggctgaccgg cgctgccggt ctggcccttc tgggggggct ggttgtcgcc
     6001 gtgtttatcg agagctgggg agaaaccgtc ctgcgcccgg acctctatgc gaacgcagat
     6061 tattacctgc tgcagccggc taacctgttc gggatagcct ggtgtgccgg agggcttgtc
     6121 gccggcgtcc ttctggcgcg gctgctggcg ttcctgctgc ttgatggtca ttttgcggcc
     6181 gctgacgagg cggtgaacgc gaaactgaat caggagagcc ggcgcagcag ccagcggacc
     6241 ggagaaatga cagacgtccg ccacctgcat ttcggggagc cggtaccggt taacgcgctg
     6301 gccgattttt ccacggagca ggcccggaaa cagcaggcgg tgtttctggg taaggatgag
     6361 cagggccagc cggtgctggt ccctcgtgat acctggcgca agaccaatat tcagatcctc
     6421 ggcctgcccg ggagcggcaa gagcgcgatg gccaccaacg cgctcattcg ctgcgtacgc
     6481 gatttcgggg acgctgtggt gtacttcgac cccaaagggg acgcgtgggc gccgcacgtc
     6541 ttccgggcgc actgcccgga cttcaccctg ctggatctgc ggccggggaa accggcgcag
     6601 ctgaatctgt tccgcgatct cgaccagtac gccctgaaga atctgctggt tgccgggttt
     6661 aacctgagtg agaccggcga cgtggcggac cactaccgca tcagcgagca gaaagcggcg
     6721 aagctgattg cggagcagtt cccgcagggg gcaaacattc agcaggtgct ggcggcggcg
     6781 tacgcgttgc cggaggccct gaaaaaggac gtgaaggggc tgatcaccaa gctggagaac
     6841 gtggccgacc tgagcgtcct gcagacggac agcggtatcg atgtggcggg gataatcaac
     6901 ggcggcggct gcctgtacgt catcggctcg atggacgatg aggcggtgat ccgggtgcag
     6961 aagatgctgt ttgcccgctg cgcgcaaatc atcattgcgc gggatgagtt ccggaggtgg
     7021 ccgcacgcga gcatcatgct cgatgagatt aaatatctgc tgtcgaagta cgtgctgaac
     7081 gcgctgggga cgctgcgcag ccgggactgc aacctgctgc tcgcgcacca gtcgctgggc
     7141 gacttcggtc agtgcgggca ggatctgccc gctgattttg tgaaaaccac ggtactggat
     7201 aacacgccga tccgctggtt ctatcgcgcg gccagccagg agtccgcgca gtgggcggcg
     7261 ggccagaccg gggagatccg ggtggacgtg gagcgccgcc gggccagccg ggaggcgggg
     7321 aacgtggagc acatcagcgg cgacagcttc atccagaagg aggcgcggcc gctgtttgac
     7381 gtgaacaccc tgcaacacct gcctgacggg ttcgcggtga tgaccgggct gggcgtggcg
     7441 cggctggcgt tcagcagccc gctgcgggtt gagcgccggg aaatttcgct gaaaaccttc
     7501 ccggtgctgg cgaaaacgga cccgctggcg gagtacctgc ctccggccga cggcggcgga
     7561 ccaaaagcgg atgatgattt tgcggggctt tactgatgac actggagcag tacaacgtca
     7621 gccacgcgaa gcggcaggcg cggaacgcgg agaaaacacg gctgaccctg cgctggctgc
     7681 gggaagagct gtgttccacg gcggagctgg ttgcgcgacg gctggggatt gcggcggtac
     7741 agccggttta ccgcttcctg gacagtctgg tggcgaaagg gctgctggtc cgggcgaaat
     7801 acccggttga cggccgtcag gtcagcgtgt gggggctgac gccgcacggg gtggcgttct
     7861 cgttcgacga ggacgagccg ctgacggacg ttattccgtt tcagccgtca agggtgtcgg
     7921 cggcgcagct gccgcaccgg ctggccgtgc agtccctgcg cctctccatg gagggccggg
     7981 gggccaccgg ctggcggtac ctgcaccgta tggccctgaa agggatgaaa gtgccggacg
     8041 cgctggcgga gctggacggc agaacggtgg catttgaggt agagcgcacc gtcaagagcc
     8101 gcaggcggta tcaggaagtg gtgtccgggt atctgttcaa ccgccgggcg aacggcatcg
     8161 atgaaatctg gtacatctgc cctgaccggc cgacgcttgt ccgggtacag cgggcgattc
     8221 tggcggtgga tgaaatcgtg aatccacaga ccggcgaggc ccgcaaaacg gtggagctgg
     8281 accgggagag gctgttcgcg tgctttaaat ttatgacaac ggagtgaggg attatggcgc
     8341 tgtacatggt atttattgag gacgtggaag acaaagacgg cgagcgctgc attatgccaa
     8401 ccagcgagca tctggaagac atggtggtgc tcgcctggca gaccggggcg tatgttttct
     8461 gttttgtcga ccgggcccag catctggcga tgatgaaatc catcgggatg tatgacgagc
     8521 ggctcaacag cgacgaaaaa gccacgtact ttttcaccgg tgaaaatccg caatgggaac
     8581 gggacaaacc cacaggaatg aagctgtgtg attacctcta tcgaaaggtg gagcggttcg
     8641 ccatcgagaa cggtgtgcag gtggttgatg ccgggaagtg ctcaccggag cagattaacg
     8701 cgatgattga ggtggtcagc gagcggcacg gggaaagcgc ggtgaacgag aagctgaaag
     8761 cggagctgat gcgggaatat aacagccgct attttaacgt gccggtgcct gaactgccgg
     8821 aggacaaacc cgcccgccgg tggtggccgt tcggcaaaaa ataacgccag caccggcatg
     8881 gccggtgctg ttattcttcc ccgagcttat cccgcatcac ctgccagagt gtttccgcat
     8941 cctcgtgaag ccgttcgcac atgtcggccg gggtatccat gtccagctcg ttcagctttt
     9001 ccagcaacag cagcgactga tccttaatga atttagccat attgagggca gttttttcct
     9061 gcttcgtcat gttctccatt cccttcggta agttctcagc taaaccagcc ggaccagaag
     9121 cgttttttct tctgtgtcag ttctgttgtc aatctgtcca cttcggcctg gagtttccgg
     9181 cgctcatcat cgtgctggcg gcgttcttct tcccgggatg ttttatcttc cagcatcagg
     9241 gtgacagcct gccggagatc ggccagttcc ttttgcattg ccagaagctg ttcctgtggg
     9301 aaattttttt gtgtattttc acggtgtgaa atgccgggaa tttgtttctc agggatattc
     9361 acatttccgt atacacggat cagttctgac acatccacga cagggttgtt tttcccgtca
     9421 cgtgacacgg taacctttcc ctgttttaca tgattgtata gcgttcttct ggtaatgccc
     9481 gcggcttgtg ctgcctgtga aaggttgagc aatgttttcg ccactgtata cccctgattt
     9541 tatcgggtgt gaaaggtgtg taatttctca cggtaaatgt atcgctgaat tttacccgtt
     9601 tttccgttca ggatgtggtt ttctgacgcg ctgcgcttgt caaaccggcg acggccacgc
     9661 aatgaattgc gcccccgtca tcggttatta gaggctgtct gcccccggat acccggattc
     9721 tttctgatgc ctgacggtga cgatcacaat ctggtccatt tcccggtcgt ggcggtagag
     9781 catcacatag ccactgtcgc caaacccgat caccagttcc ttatattcca gcggcagaaa
     9841 cggcaccggg cggccaatgt ctggcaatgt tttcagttgc tggatagccc ggacgatcac
     9901 ctcacctcct tttctggctg ccagccgatt tttggttttg agaaagtcct gaaggcgttg
     9961 taaatcgcgc tgtgccagcg cggaaatcgt tacctgtggc atggcggagc atcctgctcg
    10021 ttctcagttc cccaggtact tatccaggct tccgcttctt ccgcagtaag gtgcagaccc
    10081 gtttcctgat agtgctgcca tgcggcctgt ccgtcccgca ggtactggct gcgcttctct
    10141 tcccggtcga tgtattcggt gattgcctcc agcattagcg cgtgcgctga gcggtgccgg
    10201 tcatcggcca gcgttttcag ccggtcctta agttcttcat cgagcctgat ggatgttgct
    10261 gatgacataa aagccccttt gtagtcatat gtattacaca tgactacttt aatccgtggc
    10321 ggcgctattt gccagaaata cggcttttgc tgcccatttt tcagcttcgc ttccactaat
    10381 cagggctgct acaggctcac tcatcatcca ggactattgc agcctgcggg ttaaaggacc
    10441 tgttactggc ggcaaccatc atcattgagc ttttgggtgg tgactggtcg cggatcctgt
    10501 aagccgtcat ttttcagact gaattcaaaa tttataacct gtcgcattgc cgggtgccct
    10561 gcgggatata gccagtgcct cattgccgtc agcgtttccc gaaaaaaaag gtgacagccg
    10621 gtaagcgaca cgttgtccac tcgtccatcg tccagaatat caaacgtggc ggtcacctga
    10681 ccttcgatgc gttttgctgc ggcctgtacc gggtaggttg gctgcagtgt ggttacgggt
    10741 tttagtggta tcgcatgtac cggagtgagg aacatggccg ccagcactgt cagagtaaaa
    10801 gctttcatgg tctgaaatcc agaggggcgt ttttgaatat ttgccaggta ttttcatccg
    10861 gtgccggcgg aattttggcg tgggtaacgg ctgatattgc tgctttacag agtttcggat
    10921 ctcctccttc agcaatggcg ctgttcagag tgccatcacg ttgtatggat atccgcacgg
    10981 tgcacatttt tcccgcatag gcctctggtt caaacatctg catttcaaca gcggtcttga
    11041 tggccgcagc gtagctactg attttagatg acttatccag agtataaatt ttcggcggct
    11101 gggtggtgga atgcttacag gccgtcagga gtaaggggct gcacaatgcc agcaggataa
    11161 agggtattaa acgcatcggg ttgatgacct gtcagtaaaa aatgttacat gtatatttgt
    11221 cactctttac ggccacgttc atttacataa acacggctta tcgtatcgtt tttacaccaa
    11281 acggcccgcc atgcagttgc gtgcataggc atgcagcggg ttacaattta ccgggcgcgt
    11341 ttccggcggt tttccgggtg gtttgttgcc tgttttatcc cgtagccgcc ggaaacgccc
    11401 tcagaccgtc tgagcggtgc gggcaatgcg acgttatggt aaatacccct taccggcggc
    11461 gctgtggctg ccaggtgaag gtggtttccg tagcctcgta tttctcccga cgcatttcct
    11521 gcgcccgcct ggcttcacgt tcgtcctgaa tggcacgaag caccctttcc cgcacctgtt
    11581 gttttaccgc ctttggcagc gttttgttcc ctttcaggcc catggccagt gcctgagcga
    11641 caaaggcgtc cgccaggcgc ttttcttcag cgggagtcag tttcaggtgc gtcatagtgg
    11701 cctccgggcg tctcaggttt cccgaacagt ttaaccgcag gtcctctcct catgccagtt
    11761 agctttcaga tgtgtctata ccatctgaaa tgtaagtgtg atgaatgtct taaataataa
    11821 aatttgttgc catgagaaat tttcgggaaa agacgcgatc tgcttacagg tggctccggg
    11881 cgcttttttg tgcctgaaga tgagaactaa tctcatatat tgacacctga aaacgggagg
    11941 agtaaggtaa taattatact gtgtatatgt
//
