LOCUS       NZ_CP119155             6303 bp    DNA     circular CON 20-APR-2024
DEFINITION  Enterococcus faecium strain GENOMIC22-005 plasmid pCFSAN126950_05,
            complete sequence.
ACCESSION   NZ_CP119155
VERSION     NZ_CP119155.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33438667
            Assembly: GCF_029167665.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Hoffmann,M., Luo,Y., Sorensen,L.H., Pedersen,S.K. and
            Hendriksen,R.S.
  TITLE     Results of the 2020 Genomic Proficiency Test for the network of
            European Union Reference Laboratory for Antimicrobial Resistance
            assessing whole genome sequencing capacities
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Hoffmann,M., Luo,Y., Sorensen,L.H., Pedersen,S.K. and
            Hendriksen,R.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2023) CFSAN, US FDA, 5001 Campus Drive, College
            Park, MD 20740, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbial Genome Analysis v. SMRT Link
            11.0.0.146107
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 700.0x
            Sequencing Technology             :: Pacbio Sequel IIe system
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029167665.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 06:43:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,199
            CDSs (total)                      :: 3,108
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 43 of 140
            Pseudo Genes (incomplete)         :: 88 of 140
            Pseudo Genes (internal stop)      :: 37 of 140
            Pseudo Genes (multiple problems)  :: 21 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119155.1.
            Bacteria and source DNA available from Sorensen, Lauge Holm,
            lahoso@food.dtu.dk.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="GENOMIC22-005"
                     /isolation_source="EQA test strain"
                     /db_xref="taxon:1352"
                     /plasmid="pCFSAN126950_05"
                     /geo_loc_name="Denmark"
                     /collection_date="2022"
                     /collected_by="DTU National Food Institute"
     gene            join(6165..6303,1..32)
                     /locus_tag="P0D82_RS15790"
                     /old_locus_tag="P0D82_15785"
     CDS             join(6165..6303,1..32)
                     /locus_tag="P0D82_RS15790"
                     /old_locus_tag="P0D82_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            482..1015
                     /locus_tag="P0D82_RS15795"
                     /old_locus_tag="P0D82_15790"
     CDS             482..1015
                     /locus_tag="P0D82_RS15795"
                     /old_locus_tag="P0D82_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            1027..1230
                     /locus_tag="P0D82_RS15800"
                     /old_locus_tag="P0D82_15795"
     CDS             1027..1230
                     /locus_tag="P0D82_RS15800"
                     /old_locus_tag="P0D82_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            1552..1932
                     /locus_tag="P0D82_RS15805"
                     /old_locus_tag="P0D82_15800"
     CDS             1552..1932
                     /locus_tag="P0D82_RS15805"
                     /old_locus_tag="P0D82_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1914..2828
                     /locus_tag="P0D82_RS15810"
                     /old_locus_tag="P0D82_15805"
     CDS             1914..2828
                     /locus_tag="P0D82_RS15810"
                     /old_locus_tag="P0D82_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3073..3762
                     /locus_tag="P0D82_RS15815"
                     /old_locus_tag="P0D82_15810"
     CDS             3073..3762
                     /locus_tag="P0D82_RS15815"
                     /old_locus_tag="P0D82_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            4645..5595
                     /locus_tag="P0D82_RS15820"
                     /old_locus_tag="P0D82_15815"
     CDS             4645..5595
                     /locus_tag="P0D82_RS15820"
                     /old_locus_tag="P0D82_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            5616..6149
                     /locus_tag="P0D82_RS15825"
                     /old_locus_tag="P0D82_15820"
     CDS             5616..6149
                     /locus_tag="P0D82_RS15825"
                     /old_locus_tag="P0D82_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     Typing          4647..5595
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1915..2825
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagaattgtt tgtaaaagaa acagataaat aataaaaaag actgctcttt cgagcagcca
       61 gttaaaaaat aatgagtgtt tattcttgaa aaagcaacgc gcttatgaag cgctaccaaa
      121 aatttatcat attttattaa aaataacaac ccagtaaaaa atgactggta aaaattcaca
      181 aagaaacgga aagaagcaac cgcagaaaat caagcttaaa aagagctttt gacctagacc
      241 aatcgaaaat cacaaagtga gcgcctgtat ctattgctac aggcgctact tggcatagtg
      301 tacattatgt caacaagaat ttaatataac atattttcct aatgcattct aactatacca
      361 cattatcaaa aatataaatt atctttacta aataaaatat tgacaagcct ataccttttg
      421 ttttatgatt ttcacaaaga aaacgttttc tatatatttt tctgtatcag gaggaaattc
      481 tatgaaaaaa tttactttag gggtagcttt gttaactatt ggggcagttg ttttaccgtc
      541 aactactata gtttatgctt cagaaaattc ccaagtggta tcattaaacg aaaacaatgc
      601 tactgaaaat gtagttattg agtacactga agatggtatg gtcgtaacta aatatgagga
      661 tggttatagt gcggaagatt ttgcaaattc ttcagatatc atgacaagag ctgcagttgc
      721 aagatggggc aaaaaggaat atacaaatat tgctgtaacc actggagtag ctgctaatgc
      781 tattaatgct gccttttggg caggagggaa agctagcact tctatgtttg gtatcccagg
      841 ttgggcaatt ggtgctttgt taacaggtgc aagttggact aaaaaaggtg cttctccagg
      901 gaatgcagtt gcaaaagaat gggacaaaaa tggtaatggc tggattggat tttattacca
      961 aaggggatat gatgcagctg gaagagttgt agctactaga tattcaacgt tataagttaa
     1021 ggagtgatgt ttttgatatc taaatcatta aaatcattaa aaacatttac taaaaagaat
     1081 ccgcaaatag ctactattcg tatttttata tttgctatag ctgttgctat aattgttaat
     1141 atgctaagcg gagggcaatt atacaaatct ggtttatttt tagtgttaat cttagttgtc
     1201 ttagacataa taaagtctaa aaaaaaataa atcgatacat tttagaagaa tagggttgtt
     1261 tgaatattac aaagcaacaa tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg
     1321 gctagcaaat gctagccttt ttttgtattg tggagcgtgg attttgaggg ggattcaagg
     1381 gggagcttgc gctcctttga caagcggtta tttgcaccaa atacggaggt atttggctag
     1441 caaatataga gcttgccaaa cccttttagt gagctttact cattgtacct ttttaggtac
     1501 aatggtattg cgaacgatac gtccgtagga cgtactgaaa gggtgggact tatgagcgaa
     1561 caacatcaaa atttggctag cgatcattct aaaaaatata cctatcgatc cgagccaaaa
     1621 caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc tgaagcagtc agctgaagct
     1681 ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac aaaacgcgcg tctggtgaca
     1741 ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg atttagccaa agctggaggg
     1801 aatatcaatc aaattgccaa gtggtgcaat acacaccaac acgacgttgc tcctgatgat
     1861 gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg agctacaaaa gatatggcaa
     1921 cagttaaagt aagtcgaacc acttcatgta gccgagcaat caattacgca gaaccacgag
     1981 caaccgtcaa aacaggaata aactgtgaca ttgattatgc caaaagtgaa atgaaacaaa
     2041 tacgcacgct ttatggaaaa gacgatcgtg tacaagcaca cttattgatt cagtcctttc
     2101 gaccaggaga aattaccgct gaaaaagcca atcaactagg ggcagagtat gcgaaaaaaa
     2161 tagcccctaa ccaccaaatc gcgatttaca cacatacaga caaagaccat attcataatc
     2221 acattgtcat taactccgtt aatcttgaaa ctgggaaaaa atttcaagcc catgggcaag
     2281 catttttaaa caaatgttat gacctaaatg acgagatttg tcttgaacat gacttaacaa
     2341 tcacggaacg aggaaagaaa accgaaaaac gaaccatgtc tgaaattaaa ttaaaagaaa
     2401 aaaatgaacc ggtttggaaa gacgaaattc ggtttgcgat tgatcaaaca atgaaaaaca
     2461 aaaaaactcg aacgtatacc caattttgtg attcacttaa aacattcggc attcattgct
     2521 tgaatcgcgg aaaaaatttt acttacgaat tggtccacaa aaagaaaaaa gtacgatcca
     2581 acaaactagg aaaggattac gaaaaggaga cgattttaca tgagctggac agacgagaac
     2641 gaacctacaa tcaacaacga accaacgagc tacgaagagc tcgagctagc attcgcgaaa
     2701 ctgcagaaga agtacgaaga gaacaacgaa cttttacgcc tgaacaatcg gaaattagac
     2761 cgactcgaag aaaccatgaa caacacgttg aacgaggaac agaccaccaa caagaattat
     2821 cgctctaaaa cgttaaaaga catcgaaaac gaaataaaga gacaacgtgc ctttcttgcg
     2881 ccttataccc aagaaaggat acaaactacg atggattcac aagtcaaaaa actccaccaa
     2941 gcgctattag accagcgaaa ccaagaacaa aaaaaggctt taattcaacg atcgatggat
     3001 attcttgccc ccttatgttg cttagtacta acattattag ccttttggtt ggtatgggga
     3061 gaataaacag acatggcatt ctatttaccc tatctactca tttctgccag tattttagga
     3121 attatttggc ttagctataa ggcttatcaa acaagatatt cattaaaagg ctctaaaatt
     3181 cgatttaagc gatttttatt ggtcggggtt atatttgcct tatcaatcgt tttaagtggt
     3241 ttattcggcg ttttagaggg taataaacgt gtttcaagaa gcattttatt aggggatatc
     3301 actcaaaagt atgaagcagc atgtgataaa aagaaaaaag agcaagcatt agcacaaaaa
     3361 atggaggaat ttaccgcttg ttatgaggac atgaacaata tttttgtgga tcaagagaaa
     3421 cgtttaacag ataaaaacat gaaaaaactc actcgttttt atcaaaactt accagaaaaa
     3481 cagcaaaaag aggtacaaga aaaatatgag caagtaaaaa aagacgtgca atattttaaa
     3541 gatacgcaga cagaagaagc ttgttgtaac ttatttagcg atacaatccc tttttcaacc
     3601 agtgaacaag aacgaaaaga acggcaacaa tcggtcacat acgaacgtta tgaaacactg
     3661 cttcagcaag caacaaacat acaaaatcca acgaaaaaag aaacggcatt aaattatcta
     3721 gaaagtgtca aggaatggtt ggatcagcaa caacaaaatt gaatttgatt agcaatcatg
     3781 ttaaactgaa cctgcaaatg atcaaacatt agatacacga aagccccaga gaggtcggat
     3841 tctctggggc tttttttcag gttgatgtcg cctttattga ttttacttct tgtttcggtt
     3901 attcaaccaa taactgaata aagagacaca aacttctaca agaagggggc aaatgaaccg
     3961 ttcaaacatt agatacacct ccttccagtc gcaagactgt cggataggcg acaagttatt
     4021 ataccttaca aaataaaatc tgtgtggtgt ttttaagtat cccttatttt cgggttgtta
     4081 gggcttgccc tgaccgcctg taaggcgctt taagaacaag ataaggtttt ttacccaaga
     4141 ctgtcttaaa acgacttaga cgagctatca gaggcttccc ccacctccgt tactgacttg
     4201 tttttagcaa aggagccttt aggcgactgc gcagtacaat gtgagagcat agcgatcaca
     4261 cttttttagc gaccgtaggg agcaaggttt tgaattggca gttttgaaaa atgcgcgaac
     4321 ctgttggtag aaaaaagaag tggtttttag gcgggaaatt ttgtcaccct cttttataat
     4381 ctctttttaa accttttttt aaacctcttt accgtcggct gtgtccttac tccttcaatg
     4441 gatacagcgt tttataaact acattttgtc tgcttttaaa ctacattttg tctgctttta
     4501 aactacattt tgtctgcttt taaactacat tttgtctgct tttaaactct ttttttgcac
     4561 tcacgtatgt tataatatgc attatcacag acaaaacgtt gtttataacg tctgtatcca
     4621 ttgaaggagt aaggaggaat atctttggca aatgaaatcg tgaaatacca tcatgaatta
     4681 aataccattc cgttgcggaa atttacacca gttgaaatga atctattttt ctctattgtg
     4741 tctagaatgc gcgaaaaagg cgataaaaca gtccgtctta cttttgacca actaaaagat
     4801 ttaagtaatt ataaagcaac tgctaataat cgtttcatta atgatttaga aatgacttat
     4861 gataaattaa tggatttacg ttttggtaaa agaagtgcta atgggctcga tatagataga
     4921 ttcgttatgt ttagtgagtt taaaatacgt ggaactgcta aagatccata tgtagacata
     4981 caagtttacg aaaaagcttt acctttacta aacaatttag atgagtgggt tcgctattca
     5041 ctccaacaat ttaacgaatt acaaagtagc tatgcaaaga ctatgtttcg attactaaaa
     5101 caatatcgaa ctaaaggttt tgcttatttt tctaaagagg attttcatga actattagat
     5161 attccaaaaa gttactggaa caaccaagca aacgttgata atcgtgttat aaagcctata
     5221 cgacaggaat taaccgcaat cttcaaagga ttatcaatca aaaagaaata cggaaaaggg
     5281 cgaggaaaac ctgtcgttgg ctatcaattt acgtttaaac cagaaatcaa aaaagcagat
     5341 gattttaata aacatgcacc aaccaaagaa gaacaaaata aagcattggc tttagctgat
     5401 aaacaaaaag aatacgaaga acctgctaca acaattaacg aaaaaatcaa acagaagaaa
     5461 cgagaacttg aactcctcca acaaatcaaa caactagaaa aagagaaaga agctcttgaa
     5521 aaacaaacag agtccgcact ttcgaaaaaa gagaaaaaaa gtttattatc tgatttagat
     5581 aatttattcc agtagcaacc taggaggcaa ccagtatgca accacaagaa acctttacaa
     5641 taaaggaatt gtcagaactt tttaagatga gtaggcaagc aatatcgaaa catatccaaa
     5701 aattagattc ttccatgata gctaaaaatg agcgtggtta taaggttgta ttgcgttctg
     5761 gagtattgca acttgctcgc aacttaggca accaaatgtt gctagatagg ttgcacgagt
     5821 acgaacaaga caacgataaa aatgttaata taccaagcaa aaacgtggtt acaaacaagt
     5881 tgctagatca gttgcgtgaa gaaaatgaat ttttacgaga acaacttgcc ctcagagagc
     5941 aaaataattc tcatatacaa aaactactcg accagcaaca acaactcacc ctacaagcca
     6001 acaaacaaat cgaaaaactg caagaaaaac tacagttgac ttatacagaa gaagctccag
     6061 agaatgactc aacgactctt tctgaagcaa cagaaacaat agacaaacaa attgatactg
     6121 aaaaaaaatg gtggcaactt tggaaataac tggagggaac attcatgaca agaaaaaatc
     6181 ttctattcgg ttctatttta ctaggaattc taggattcag tccgatgtta ttgtcagtga
     6241 tcaataaagg gaataaacat ggtgaattga aattgaatcc aaattattac aacagtccta
     6301 acg
//
