LOCUS       NZ_CP119154            12346 bp    DNA     circular CON 20-APR-2024
DEFINITION  Enterococcus faecium strain GENOMIC22-005 plasmid pCFSAN126950_04,
            complete sequence.
ACCESSION   NZ_CP119154
VERSION     NZ_CP119154.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33438667
            Assembly: GCF_029167665.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12346)
  AUTHORS   Hoffmann,M., Luo,Y., Sorensen,L.H., Pedersen,S.K. and
            Hendriksen,R.S.
  TITLE     Results of the 2020 Genomic Proficiency Test for the network of
            European Union Reference Laboratory for Antimicrobial Resistance
            assessing whole genome sequencing capacities
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12346)
  AUTHORS   Hoffmann,M., Luo,Y., Sorensen,L.H., Pedersen,S.K. and
            Hendriksen,R.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2023) CFSAN, US FDA, 5001 Campus Drive, College
            Park, MD 20740, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbial Genome Analysis v. SMRT Link
            11.0.0.146107
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 700.0x
            Sequencing Technology             :: Pacbio Sequel IIe system
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029167665.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 06:43:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,199
            CDSs (total)                      :: 3,108
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 43 of 140
            Pseudo Genes (incomplete)         :: 88 of 140
            Pseudo Genes (internal stop)      :: 37 of 140
            Pseudo Genes (multiple problems)  :: 21 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119154.1.
            Bacteria and source DNA available from Sorensen, Lauge Holm,
            lahoso@food.dtu.dk.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="GENOMIC22-005"
                     /isolation_source="EQA test strain"
                     /db_xref="taxon:1352"
                     /plasmid="pCFSAN126950_04"
                     /geo_loc_name="Denmark"
                     /collection_date="2022"
                     /collected_by="DTU National Food Institute"
     gene            join(12280..12346,1..104)
                     /locus_tag="P0D82_RS15700"
                     /old_locus_tag="P0D82_15695"
     CDS             join(12280..12346,1..104)
                     /locus_tag="P0D82_RS15700"
                     /old_locus_tag="P0D82_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032491833.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            559..783
                     /locus_tag="P0D82_RS15705"
                     /old_locus_tag="P0D82_15700"
     CDS             559..783
                     /locus_tag="P0D82_RS15705"
                     /old_locus_tag="P0D82_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="WP_002363584.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="class II bacteriocin"
     gene            803..1090
                     /locus_tag="P0D82_RS15710"
                     /old_locus_tag="P0D82_15705"
     CDS             803..1090
                     /locus_tag="P0D82_RS15710"
                     /old_locus_tag="P0D82_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_002363589.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     gene            1540..1920
                     /locus_tag="P0D82_RS15715"
                     /old_locus_tag="P0D82_15710"
     CDS             1540..1920
                     /locus_tag="P0D82_RS15715"
                     /old_locus_tag="P0D82_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_010782660.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1902..2816
                     /locus_tag="P0D82_RS15720"
                     /old_locus_tag="P0D82_15715"
     CDS             1902..2816
                     /locus_tag="P0D82_RS15720"
                     /old_locus_tag="P0D82_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010782659.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2776..3054
                     /locus_tag="P0D82_RS15725"
                     /old_locus_tag="P0D82_15720"
     CDS             2776..3054
                     /locus_tag="P0D82_RS15725"
                     /old_locus_tag="P0D82_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369881.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDSQ
                     VKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            3061..3750
                     /locus_tag="P0D82_RS15730"
                     /old_locus_tag="P0D82_15725"
     CDS             3061..3750
                     /locus_tag="P0D82_RS15730"
                     /old_locus_tag="P0D82_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369882.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            4594..5538
                     /locus_tag="P0D82_RS15735"
                     /old_locus_tag="P0D82_15730"
     CDS             4594..5538
                     /locus_tag="P0D82_RS15735"
                     /old_locus_tag="P0D82_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            5559..6092
                     /locus_tag="P0D82_RS15740"
                     /old_locus_tag="P0D82_15735"
     CDS             5559..6092
                     /locus_tag="P0D82_RS15740"
                     /old_locus_tag="P0D82_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002363576.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            6107..6277
                     /locus_tag="P0D82_RS15745"
                     /old_locus_tag="P0D82_15740"
     CDS             6107..6277
                     /locus_tag="P0D82_RS15745"
                     /old_locus_tag="P0D82_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032491833.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            6732..6956
                     /locus_tag="P0D82_RS15750"
                     /old_locus_tag="P0D82_15745"
     CDS             6732..6956
                     /locus_tag="P0D82_RS15750"
                     /old_locus_tag="P0D82_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="WP_002363584.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="class II bacteriocin"
     gene            6976..7263
                     /locus_tag="P0D82_RS15755"
                     /old_locus_tag="P0D82_15750"
     CDS             6976..7263
                     /locus_tag="P0D82_RS15755"
                     /old_locus_tag="P0D82_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_002363589.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     gene            7713..8093
                     /locus_tag="P0D82_RS15760"
                     /old_locus_tag="P0D82_15755"
     CDS             7713..8093
                     /locus_tag="P0D82_RS15760"
                     /old_locus_tag="P0D82_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_010782660.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8075..8989
                     /locus_tag="P0D82_RS15765"
                     /old_locus_tag="P0D82_15760"
     CDS             8075..8989
                     /locus_tag="P0D82_RS15765"
                     /old_locus_tag="P0D82_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010782659.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8949..9227
                     /locus_tag="P0D82_RS15770"
                     /old_locus_tag="P0D82_15765"
     CDS             8949..9227
                     /locus_tag="P0D82_RS15770"
                     /old_locus_tag="P0D82_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369881.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDSQ
                     VKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            9234..9923
                     /locus_tag="P0D82_RS15775"
                     /old_locus_tag="P0D82_15770"
     CDS             9234..9923
                     /locus_tag="P0D82_RS15775"
                     /old_locus_tag="P0D82_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369882.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            10767..11711
                     /locus_tag="P0D82_RS15780"
                     /old_locus_tag="P0D82_15775"
     CDS             10767..11711
                     /locus_tag="P0D82_RS15780"
                     /old_locus_tag="P0D82_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            11732..12265
                     /locus_tag="P0D82_RS15785"
                     /old_locus_tag="P0D82_15780"
     CDS             11732..12265
                     /locus_tag="P0D82_RS15785"
                     /old_locus_tag="P0D82_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002363576.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     BGC             1..11956
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4595..5538
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          10768..11711
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1903..2813
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8076..8986
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tatcagtggc caacaaagga aataaaaaag gggaactaaa actcaatcct aattactaca
       61 acagtcaaaa tgaggaagta tttgtagaaa aacttgataa ataagaaaag actgctcgta
      121 cgagcagtct ttctgaaaga gattatccta ttattaacct tagaaaagaa taataaatga
      181 atatgagata tgtaaaaagc ataacaaaca caaacaaaaa taacaagtat ttaaaaaatg
      241 accgatcaat attcacaaag aattagaaag aaacaaccgc agaaaaccaa gccttaaaaa
      301 gagcttttaa cctagaccaa taaaaattca caaaatcagc gcctgtatct attgctacag
      361 gcgttacttg gtagtataca cattatgtct actagctgtt taaataactg atcttataat
      421 tttatttaaa cgattatgaa ttcaaaacta ctttttatga cgttaatcat tgttatataa
      481 taaaatgtta tataataaaa ttactaagat tatttaatat aacaagcaat tttaaaaaaa
      541 cgaaaaggaa aaacaatcat gaaaaagaaa gtattaaaac attgtgttat tctaggaata
      601 ttaggaactt gtctagctgg catcggtaca ggaataaaag ttgatgcagc tacttactat
      661 ggaaatggtc tttattgtaa caaagaaaaa tgttgggtag attggaatca agctaaagga
      721 gaaattggaa aaattattgt taatggttgg gttaatcatg gtccatgggc acctagaagg
      781 tagtggtagg gaggttatta taatggattt tactaaagaa gaaaaacttt taaatgcaat
      841 tagtaaagta tacaatgaag caactataga tgactatcct gacttaaaag aaaagctctt
      901 tctttattct aaagaaatca gtgagggaaa aagtgttggt gaagttagta tgaaattaag
      961 tagttttctt ggaagatata ttttaaaaca taaatttgga ttacctaaat ctttaataga
     1021 attacaagaa attgttagta aggaatctca agtatataga ggatgggctt ctattggtat
     1081 ttggagttaa ttctttggaa taaaatgaaa atgggactga agaatcaatt ctgattcatc
     1141 agtcccataa attatagagc cgaaaaatta tcatatttat agatttatta atagtaaatg
     1201 gcataagaat ctttgaacaa cgaaaaaaat gtagctctac ctaattattg atttgcaaca
     1261 atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt tgctagtctt
     1321 ttttgtattg tggagagtgg gggggagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtga gctttagtga gctttactta ttgtaccttt ttaggtacaa tggtattgcg
     1501 aacgatacgt ccgtaggacg tactgaaagg gtgggactta tgagcgaaca aaatcaatat
     1561 ttggctagca atcattctaa aaaatatacc tatcgatccg agccaaaaca aatcagtttt
     1621 cgagtgagcg aatccgaatt tgcaaagctg aagcagtcag ctgaagcttt gcaaatgagt
     1681 gtgccggctt tcgtgaaagc caaggcacaa aacgcgcgtc tggtgacacc gaaagtggcg
     1741 ccagacattg ctcaggcaat cgcgcgcgat ttagccaaag ctggcgggaa tatcaatcaa
     1801 attgccaagt ggtgcaacac acaccaacac gacgttgatc ctggcgacgc ccaacgctta
     1861 tccgaaaacc taaaaatcat gcaaaaggag ctacaaaaga tatggcaaca gttaaagtaa
     1921 gccgaaccac ttcttgtagc cgagcgatca attacgccga accacgagca accgtcaaaa
     1981 cagggataaa ctgtgacatt gattatgcca aaagcgaaat gaaacaaatt cgcatgctgt
     2041 atggaaaaga cgatcgtgta caagcacact tattgatcca gtcttttcga ccaggagaaa
     2101 ttaccgctga aaaagccaat caactagggg cagagtatgc gaaaaaaata gcccctgacc
     2161 accaaatcgc gatttacaca catacagaca aagaccatat tcataaccac attgtcatta
     2221 actccgtcaa tctggaaact gggaacaaat ttcaagccca tggtcaagca tttttaaaca
     2281 aatgttatga cctaaatgac gagatttgtc ttgaacatga cttaacaatc actgaacgag
     2341 ggaagaaacc cgaaaaacga accatgtctg aaatcaaatt aaaagaaaaa aatgaacctg
     2401 tttggaaaga cgaaattcgg tttgcgattg atcaaacaat gaaaaacaaa aaaactcgaa
     2461 cgtataacca attttgtgat tctcttaaaa cattcggcat tcattgcttg aatcgtggga
     2521 aaaattttac ttacgaattg atccacaaaa agaaaaaagt acgatccaac aaactaggaa
     2581 aggattacga aaaggagacg attttacatg agctggacag acgagaacga gcctacaatc
     2641 aacaacgaac caacgagcta cgaagagctc gagctagcat tcgcgaaact gcaaaagaag
     2701 tacgaagaga acaacgaact cttacgactg aacaaccgga aattagaccg actcgaagaa
     2761 accatgacca acacgttgaa agaggagcag actaccaaca agaattatcg ttctaaaacg
     2821 ttacaagaca tagaaaatga aataaagaaa caacgtgctt ttcttgcacc ttataccaaa
     2881 gaaagaatac aaactacaat tgattcacaa gtcaaaaaat tccaccatgc gctattaaac
     2941 cagcgaaata aagaacagaa aaaagtaatt atgcagcgat caatggatat tcttgccccc
     3001 ttatgttgct taatcatgac actgttagcc ttttggctgg tatggggaga ataaatagaa
     3061 atggcattct atttacccta tctactcatt tttgccagta ttttaggaat tatttggctt
     3121 agctataagg cttatcaaac aagatattca ttaaaaggct ctaaaattcg atttaagagg
     3181 tttttattgc caggggttat atttacatta tcaatcgttt taagtggttt attcggcgtt
     3241 ttagagggaa ataaacgtat ttcaagaagt attttattag gggatatcac tcaaaagtat
     3301 gaagcagcat gtgataaaaa gaaaaaggag caagtgttaa cacaaaaaat ggaagagttt
     3361 accacttgtt atgaggaaat gaacgatatt tttgtgaatc aagaaaaacg tttgacggat
     3421 aaaaacatgg aaaaactcac tcgtctttat caaaacttgc cagaaaaaca gcaaaaagag
     3481 gttcaagaca actatgagcg aaccaaaaaa gacgtgcaat attttaaaga tacgcagaca
     3541 gaagaagctt gttataattt attcagtgat acaatccctt tttcaactag tgaacaggaa
     3601 cgaaaagaac ggcaacaatc agttacctac gaacgttata aagcactgct tcagcaagca
     3661 acgaacatac aaaatccaac gaaaaaagaa acggcattaa attatctaaa aagtgtcaag
     3721 gaatggttag atcagcaaca gcaaaattga atttgatcgg caatcatgtt aaactaaaca
     3781 tgcaaatgat caaacattag atacacgaaa gccccagaga ggtcggattc tctggggctt
     3841 tttttcaggt tgatgtcgcc tttattgatt ttacttcttg tttcggttat tcaaccaata
     3901 actgaataaa gagacacaaa cttctataag aagggggcaa atgaacaatt caaacattag
     3961 atacacctcc ttccagtcgc aagactgtcg gataggcgac aagttattat atcttaaaaa
     4021 attaaacttg tgtggtgttt ttaagtatcg cttattttag ggttgttagg gcttgccctg
     4081 accgcctgta aggcgcttta aagctagata aggattttta tccaagactg tcttaaaacg
     4141 gcttagataa gcattcagaa ccgctcaaag gcctctttac caacatgttt ttagcaaagg
     4201 agcctttagg cgactgcgca gtacaatgtg agagcgtagc gatcacacct ttttggcgac
     4261 cgtaggaagc aaggttttgg attggcagtt ttgaaaaatg cgcgaacctg tttaaagaaa
     4321 aaagaagtgg tttttaggcg ggaaaattag cccccctctt tttatatctc tttttaaacc
     4381 tctttttaaa cctctttacc gtaagctgtg tccttactct accaatggat acagaatgtt
     4441 atgaaggaca ttttgtctgt ttatgaagga cattttgtct gtttatgaag gacattttgt
     4501 ctgtttatga aggacatttt gtctgtttga acgtcaaata tgaatgtctt gacatttaaa
     4561 aaatgtcatt cataattgga ttgaggtgat tatatggcta atgagattgt aaaataccac
     4621 catgaattaa atacaattcc tttaagaaaa tttacaccgg ttgaaatgaa tttgtttttt
     4681 tccatcgtgt ctagaatgcg tgaaaaaggg gataagacag ttcgttttac gtttgaccaa
     4741 ctaaaagatt taagtaacta caaagcaact gcaaatgtac gatttgtaga agatttaaaa
     4801 gcaacttatg aaaaaatctt atctttacgc tttggtcgta gaagtgagtc tgggctttct
     4861 tacgatatgt ttgtaatgtt tacagaattt aaaatcaacg gggacgtaga aaatccttac
     4921 gtagatatac gaatttacga aaaagcactg cctttgttaa ataatttaga cgagtgggta
     4981 cgctattctt tacaacaatt caacgagcta caaagtggct atgcaaagac tatgtttcgt
     5041 ctgttaaagc aatacagaac caaaggtttc gcatatttct ccaaagaaga ctttcttgaa
     5101 ttattagata ttcctaaaag ttataagcaa cctgatatag ataaacgagt tataaaacct
     5161 atacgtcaag aactaactgc aatcttcaaa ggactaacaa tcaaaaagaa atacggaaaa
     5221 ggacggggaa aacctgttat tggctatcaa tttacgttca aacctgagat caaaaaagca
     5281 gacgacttta acaaacatgc accaaccaaa gaagaccaaa ataaagcatt ggctttagct
     5341 gataaacaaa aagaatacga agaacccgct acaacaatta acgaaaaaat caaacagaag
     5401 aaacgagaac ttgaactcct ccaacaaatc aaacaactag aaaaagagaa aaaagctctt
     5461 gaaaaacaaa cagagtccgc actttcggaa aaagagaaaa aaagtttatt atctgattta
     5521 gataatttat tccagtagca acctaggagg caaccagtat gcaaccacaa gaaaccttta
     5581 caataaagga attgtcagat ctttttaaga tgagtaggca agcaatatcg aaacatatcc
     5641 aaaaattaga ttcttccatg atagctaaaa atgagcgtgg ttataaggtt gtattgcgtt
     5701 ctggagtatt gcaacttgct cgcaacttag acaatcaaag gttgctagat aagttgcata
     5761 aacatgaaca aaacaacgat aaaaatgctt atataccaaa caaaaacgtg gttgcaaaca
     5821 agttgctaga tcagttgcgt gaagaaaatg aatttttacg agaacaactt gccctcaaag
     5881 agcaaaataa ttctcatata caaaaactac tcgaccagca acaacaactc accctgcaag
     5941 ccaaccaaca aatcgaaaaa cttcaggagc aactgcagtt gacttatacg gaagagtctt
     6001 cagagaatag ttcaacgact ctttctgaag caacagaaac aatggacgaa caaacaaaga
     6061 attggaaaaa atggtggcag ctatggaaat gaagaaggaa atacttatga caaaaaaaca
     6121 cttgctattc ggttctatcc tactaggaat attaagcttt agcccaatgt tattatcagt
     6181 ggccaacaaa ggaaataaaa aaggggaact aaaactcaat cctaattact acaacagtca
     6241 aaatgaggaa gtatttgtag aaaaacttga taaataagaa aagactgctc gtacgagcag
     6301 tctttctgaa agagattatc ctattattaa ccttagaaaa gaataataaa tgaatatgag
     6361 atatgtaaaa agcataacaa acacaaacaa aaataacaag tatttaaaaa atgaccgatc
     6421 aatattcaca aagaattaga aagaaacaac cgcagaaaac caagccttaa aaagagcttt
     6481 taacctagac caataaaaat tcacaaaatc agcgcctgta tctattgcta caggcgttac
     6541 ttggtagtat acacattatg tctactagct gtttaaataa ctgatcttat aattttattt
     6601 aaacgattat gaattcaaaa ctacttttta tgacgttaat cattgttata taataaaatg
     6661 ttatataata aaattactaa gattatttaa tataacaagc aattttaaaa aaacgaaaag
     6721 gaaaaacaat catgaaaaag aaagtattaa aacattgtgt tattctagga atattaggaa
     6781 cttgtctagc tggcatcggt acaggaataa aagttgatgc agctacttac tatggaaatg
     6841 gtctttattg taacaaagaa aaatgttggg tagattggaa tcaagctaaa ggagaaattg
     6901 gaaaaattat tgttaatggt tgggttaatc atggtccatg ggcacctaga aggtagtggt
     6961 agggaggtta ttataatgga ttttactaaa gaagaaaaac ttttaaatgc aattagtaaa
     7021 gtatacaatg aagcaactat agatgactat cctgacttaa aagaaaagct ctttctttat
     7081 tctaaagaaa tcagtgaggg aaaaagtgtt ggtgaagtta gtatgaaatt aagtagtttt
     7141 cttggaagat atattttaaa acataaattt ggattaccta aatctttaat agaattacaa
     7201 gaaattgtta gtaaggaatc tcaagtatat agaggatggg cttctattgg tatttggagt
     7261 taattctttg gaataaaatg aaaatgggac tgaagaatca attctgattc atcagtccca
     7321 taaattatag agccgaaaaa ttatcatatt tatagattta ttaatagtaa atggcataag
     7381 aatctttgaa caacgaaaaa aatgtagctc tacctaatta ttgatttgca acaatttcac
     7441 cgaaggtgaa acaagcgaaa ctttacaaaa agtggctagc atttgctagt cttttttgta
     7501 ttgtggagag tgggggggag ggggattcaa gggggagctt gcgccccttt gacaagcggt
     7561 tatttgcacc aaatacgtag gtatttggct agcaaatata gagcttgcca agccctttag
     7621 tgagctttag tgagctttac ttattgtacc tttttaggta caatggtatt gcgaacgata
     7681 cgtccgtagg acgtactgaa agggtgggac ttatgagcga acaaaatcaa tatttggcta
     7741 gcaatcattc taaaaaatat acctatcgat ccgagccaaa acaaatcagt tttcgagtga
     7801 gcgaatccga atttgcaaag ctgaagcagt cagctgaagc tttgcaaatg agtgtgccgg
     7861 ctttcgtgaa agccaaggca caaaacgcgc gtctggtgac accgaaagtg gcgccagaca
     7921 ttgctcaggc aatcgcgcgc gatttagcca aagctggcgg gaatatcaat caaattgcca
     7981 agtggtgcaa cacacaccaa cacgacgttg atcctggcga cgcccaacgc ttatccgaaa
     8041 acctaaaaat catgcaaaag gagctacaaa agatatggca acagttaaag taagccgaac
     8101 cacttcttgt agccgagcga tcaattacgc cgaaccacga gcaaccgtca aaacagggat
     8161 aaactgtgac attgattatg ccaaaagcga aatgaaacaa attcgcatgc tgtatggaaa
     8221 agacgatcgt gtacaagcac acttattgat ccagtctttt cgaccaggag aaattaccgc
     8281 tgaaaaagcc aatcaactag gggcagagta tgcgaaaaaa atagcccctg accaccaaat
     8341 cgcgatttac acacatacag acaaagacca tattcataac cacattgtca ttaactccgt
     8401 caatctggaa actgggaaca aatttcaagc ccatggtcaa gcatttttaa acaaatgtta
     8461 tgacctaaat gacgagattt gtcttgaaca tgacttaaca atcactgaac gagggaagaa
     8521 acccgaaaaa cgaaccatgt ctgaaatcaa attaaaagaa aaaaatgaac ctgtttggaa
     8581 agacgaaatt cggtttgcga ttgatcaaac aatgaaaaac aaaaaaactc gaacgtataa
     8641 ccaattttgt gattctctta aaacattcgg cattcattgc ttgaatcgtg ggaaaaattt
     8701 tacttacgaa ttgatccaca aaaagaaaaa agtacgatcc aacaaactag gaaaggatta
     8761 cgaaaaggag acgattttac atgagctgga cagacgagaa cgagcctaca atcaacaacg
     8821 aaccaacgag ctacgaagag ctcgagctag cattcgcgaa actgcaaaag aagtacgaag
     8881 agaacaacga actcttacga ctgaacaacc ggaaattaga ccgactcgaa gaaaccatga
     8941 ccaacacgtt gaaagaggag cagactacca acaagaatta tcgttctaaa acgttacaag
     9001 acatagaaaa tgaaataaag aaacaacgtg cttttcttgc accttatacc aaagaaagaa
     9061 tacaaactac aattgattca caagtcaaaa aattccacca tgcgctatta aaccagcgaa
     9121 ataaagaaca gaaaaaagta attatgcagc gatcaatgga tattcttgcc cccttatgtt
     9181 gcttaatcat gacactgtta gccttttggc tggtatgggg agaataaata gaaatggcat
     9241 tctatttacc ctatctactc atttttgcca gtattttagg aattatttgg cttagctata
     9301 aggcttatca aacaagatat tcattaaaag gctctaaaat tcgatttaag aggtttttat
     9361 tgccaggggt tatatttaca ttatcaatcg ttttaagtgg tttattcggc gttttagagg
     9421 gaaataaacg tatttcaaga agtattttat taggggatat cactcaaaag tatgaagcag
     9481 catgtgataa aaagaaaaag gagcaagtgt taacacaaaa aatggaagag tttaccactt
     9541 gttatgagga aatgaacgat atttttgtga atcaagaaaa acgtttgacg gataaaaaca
     9601 tggaaaaact cactcgtctt tatcaaaact tgccagaaaa acagcaaaaa gaggttcaag
     9661 acaactatga gcgaaccaaa aaagacgtgc aatattttaa agatacgcag acagaagaag
     9721 cttgttataa tttattcagt gatacaatcc ctttttcaac tagtgaacag gaacgaaaag
     9781 aacggcaaca atcagttacc tacgaacgtt ataaagcact gcttcagcaa gcaacgaaca
     9841 tacaaaatcc aacgaaaaaa gaaacggcat taaattatct aaaaagtgtc aaggaatggt
     9901 tagatcagca acagcaaaat tgaatttgat cggcaatcat gttaaactaa acatgcaaat
     9961 gatcaaacat tagatacacg aaagccccag agaggtcgga ttctctgggg ctttttttca
    10021 ggttgatgtc gcctttattg attttacttc ttgtttcggt tattcaacca ataactgaat
    10081 aaagagacac aaacttctat aagaaggggg caaatgaaca attcaaacat tagatacacc
    10141 tccttccagt cgcaagactg tcggataggc gacaagttat tatatcttaa aaaattaaac
    10201 ttgtgtggtg tttttaagta tcgcttattt tagggttgtt agggcttgcc ctgaccgcct
    10261 gtaaggcgct ttaaagctag ataaggattt ttatccaaga ctgtcttaaa acggcttaga
    10321 taagcattca gaaccgctca aaggcctctt taccaacatg tttttagcaa aggagccttt
    10381 aggcgactgc gcagtacaat gtgagagcgt agcgatcaca cctttttggc gaccgtagga
    10441 agcaaggttt tggattggca gttttgaaaa atgcgcgaac ctgtttaaag aaaaaagaag
    10501 tggtttttag gcgggaaaat tagcccccct ctttttatat ctctttttaa acctcttttt
    10561 aaacctcttt accgtaagct gtgtccttac tctaccaatg gatacagaat gttatgaagg
    10621 acattttgtc tgtttatgaa ggacattttg tctgtttatg aaggacattt tgtctgttta
    10681 tgaaggacat tttgtctgtt tgaacgtcaa atatgaatgt cttgacattt aaaaaatgtc
    10741 attcataatt ggattgaggt gattatatgg ctaatgagat tgtaaaatac caccatgaat
    10801 taaatacaat tcctttaaga aaatttacac cggttgaaat gaatttgttt ttttccatcg
    10861 tgtctagaat gcgtgaaaaa ggggataaga cagttcgttt tacgtttgac caactaaaag
    10921 atttaagtaa ctacaaagca actgcaaatg tacgatttgt agaagattta aaagcaactt
    10981 atgaaaaaat cttatcttta cgctttggtc gtagaagtga gtctgggctt tcttacgata
    11041 tgtttgtaat gtttacagaa tttaaaatca acggggacgt agaaaatcct tacgtagata
    11101 tacgaattta cgaaaaagca ctgcctttgt taaataattt agacgagtgg gtacgctatt
    11161 ctttacaaca attcaacgag ctacaaagtg gctatgcaaa gactatgttt cgtctgttaa
    11221 agcaatacag aaccaaaggt ttcgcatatt tctccaaaga agactttctt gaattattag
    11281 atattcctaa aagttataag caacctgata tagataaacg agttataaaa cctatacgtc
    11341 aagaactaac tgcaatcttc aaaggactaa caatcaaaaa gaaatacgga aaaggacggg
    11401 gaaaacctgt tattggctat caatttacgt tcaaacctga gatcaaaaaa gcagacgact
    11461 ttaacaaaca tgcaccaacc aaagaagacc aaaataaagc attggcttta gctgataaac
    11521 aaaaagaata cgaagaaccc gctacaacaa ttaacgaaaa aatcaaacag aagaaacgag
    11581 aacttgaact cctccaacaa atcaaacaac tagaaaaaga gaaaaaagct cttgaaaaac
    11641 aaacagagtc cgcactttcg gaaaaagaga aaaaaagttt attatctgat ttagataatt
    11701 tattccagta gcaacctagg aggcaaccag tatgcaacca caagaaacct ttacaataaa
    11761 ggaattgtca gatcttttta agatgagtag gcaagcaata tcgaaacata tccaaaaatt
    11821 agattcttcc atgatagcta aaaatgagcg tggttataag gttgtattgc gttctggagt
    11881 attgcaactt gctcgcaact tagacaatca aaggttgcta gataagttgc ataaacatga
    11941 acaaaacaac gataaaaatg cttatatacc aaacaaaaac gtggttgcaa acaagttgct
    12001 agatcagttg cgtgaagaaa atgaattttt acgagaacaa cttgccctca aagagcaaaa
    12061 taattctcat atacaaaaac tactcgacca gcaacaacaa ctcaccctgc aagccaacca
    12121 acaaatcgaa aaacttcagg agcaactgca gttgacttat acggaagagt cttcagagaa
    12181 tagttcaacg actctttctg aagcaacaga aacaatggac gaacaaacaa agaattggaa
    12241 aaaatggtgg cagctatgga aatgaagaag gaaatactta tgacaaaaaa acacttgcta
    12301 ttcggttcta tcctactagg aatattaagc tttagcccaa tgttat
//
