LOCUS       NZ_CP119126             7938 bp    DNA     circular CON 05-MAY-2024
DEFINITION  Escherichia coli strain GENOMIC22-004 plasmid pCFSAN126949_06,
            complete sequence.
ACCESSION   NZ_CP119126
VERSION     NZ_CP119126.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33426038
            Assembly: GCF_029094485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Hoffmann,M., Luo,Y., Sorensen,L.H., Pedersen,S.K. and
            Hendriksen,R.S.
  TITLE     Results of the 2020 Genomic Proficiency Test for the network of
            European Union Reference Laboratory for Antimicrobial Resistance
            assessing whole genome sequencing capacities
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   Hoffmann,M., Luo,Y., Sorensen,L.H., Pedersen,S.K. and
            Hendriksen,R.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2023) CFSAN, US FDA, 5001 Campus Drive, College
            Park, MD 20740, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbial Genome Analysis v. SMRT Link
            11.0.0.146107
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Pacbio Sequel IIe system
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029094485.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 06:49:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,128
            CDSs (total)                      :: 5,008
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 279
            CDSs (without protein)            :: 279
            Pseudo Genes (ambiguous residues) :: 0 of 279
            Pseudo Genes (frameshifted)       :: 97 of 279
            Pseudo Genes (incomplete)         :: 178 of 279
            Pseudo Genes (internal stop)      :: 45 of 279
            Pseudo Genes (multiple problems)  :: 40 of 279
            Pseudo Genes (short protein)      :: 4 of 279
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119126.1.
            Bacteria and source DNA available from Sorensen, Lauge Holm,
            lahoso@food.dtu.dk.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GENOMIC22-004"
                     /isolation_source="EQA test strain"
                     /db_xref="taxon:562"
                     /plasmid="pCFSAN126949_06"
                     /geo_loc_name="Denmark"
                     /collection_date="2022"
                     /collected_by="DTU National Food Institute"
     gene            complement(289..480)
                     /locus_tag="PYZ88_RS25535"
                     /old_locus_tag="PYZ88_25490"
     CDS             complement(289..480)
                     /locus_tag="PYZ88_RS25535"
                     /old_locus_tag="PYZ88_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            545..868
                     /locus_tag="PYZ88_RS25540"
                     /old_locus_tag="PYZ88_25495"
     CDS             545..868
                     /locus_tag="PYZ88_RS25540"
                     /old_locus_tag="PYZ88_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1077..>1508
                     /locus_tag="PYZ88_RS25545"
                     /old_locus_tag="PYZ88_25500"
                     /pseudo=""
     CDS             1077..>1508
                     /locus_tag="PYZ88_RS25545"
                     /old_locus_tag="PYZ88_25500"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1546..2064
                     /locus_tag="PYZ88_RS25550"
                     /old_locus_tag="PYZ88_25505"
     CDS             1546..2064
                     /locus_tag="PYZ88_RS25550"
                     /old_locus_tag="PYZ88_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            2071..2304
                     /locus_tag="PYZ88_RS25555"
                     /old_locus_tag="PYZ88_25510"
     CDS             2071..2304
                     /locus_tag="PYZ88_RS25555"
                     /old_locus_tag="PYZ88_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3015..3254
                     /locus_tag="PYZ88_RS25560"
                     /old_locus_tag="PYZ88_25515"
     CDS             3015..3254
                     /locus_tag="PYZ88_RS25560"
                     /old_locus_tag="PYZ88_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            3251..3613
                     /locus_tag="PYZ88_RS25565"
                     /old_locus_tag="PYZ88_25520"
     CDS             3251..3613
                     /locus_tag="PYZ88_RS25565"
                     /old_locus_tag="PYZ88_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(3638..4531)
                     /locus_tag="PYZ88_RS25570"
                     /old_locus_tag="PYZ88_25525"
     CDS             complement(3638..4531)
                     /locus_tag="PYZ88_RS25570"
                     /old_locus_tag="PYZ88_25525"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(4534..6126)
                     /locus_tag="PYZ88_RS25575"
                     /old_locus_tag="PYZ88_25530"
     CDS             complement(4534..6126)
                     /locus_tag="PYZ88_RS25575"
                     /old_locus_tag="PYZ88_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(6165..6863)
                     /locus_tag="PYZ88_RS25580"
                     /old_locus_tag="PYZ88_25535"
     CDS             complement(6165..6863)
                     /locus_tag="PYZ88_RS25580"
                     /old_locus_tag="PYZ88_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     gene            complement(7411..7569)
                     /locus_tag="PYZ88_RS25665"
     CDS             complement(7411..7569)
                     /locus_tag="PYZ88_RS25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     Typing          134..213
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          complement(7498..7938)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.619"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          513..862
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
     Typing          859..2408
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgccgcagcc gaacgaccga gtgtagcgag tcagtgagcg aggaagcgga agagcgccgg
       61 aacgtgcatt ttctccttac gcatctgtgc ggcatttcac atcggacatg gtgcgctttc
      121 catacaattc gtactgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
      181 actggttcag ggcttcgccc cgaaaccccc tgacgcgccc tgaggggctt gtctgctccc
      241 ggcatccgct cacagacaag ctgttaccgt ctccgggagc tgtatgtgtc agaggttttc
      301 accgtcatcc ccgaagcgtg cgaggcagct gcggtaaagc tcgtcggcgt ggtcgtgaag
      361 tgattcacaa atatcggcct gttcatctgc gtccagttcg ttgagtttct ccagcagcgt
      421 taatgtctgg cttctgataa aacgggccat gttaagggcg gttttttcct gtttagtcac
      481 tgatgcctcc gtgtaagggg gatttctgtt ttatggggta atgataccga tgaaacgcga
      541 gaggatgctc accatacggg ttactgatga tgaacatgcc cggttactgg aacgctgtga
      601 gggtaaacag ctggcggtat ggatgcggcg ggtctgcctg ggtgagccgg ttgcacgctc
      661 cgggaagctg ccgacactgg caccgccgtt actgcgtcag ctggccgcca tcgggaataa
      721 cctgaatcag acagcgagga aggtgaacag cgggcagtgg tcttccggtg atcgggttca
      781 ggtgttggct gcgctgatgg ccatcgggga ggagctgcgt cggctgcgtc tggccgtcag
      841 ggaacagggc gcgcgggatg attgttaaat ttcatgccag gggaaaaggc ggtggcagtg
      901 gtccggttga ttacctgctg ggaagggagc gaaaccggga aggggcaacg gtgcttcagg
      961 gtaatccgga agaagtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     1021 caggtgttct gtcgttcgcg gagaaggagc tgccgccggg aggacgtgaa aaggtgatgg
     1081 cgagctttga gcgtgtactg atgcccggtc tcgaaaagaa tcagtacagc atcctgtggg
     1141 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
     1201 agaccggaaa acgcctccag ccgtactacg accgggcaga caggccgaga attgatgcct
     1261 ggcagacgct ggtgaatcac cattacgggc tgcatgaccc caacgccccg gagaaccgcc
     1321 ggatactgac tctccctgat aacctgccgg aaacgaaaca ggcgcttgca gagagcgtca
     1381 cacgcgggat agacgctctt tatcatgtcg gggagataaa aggtcgtcag gatgtgattc
     1441 aggcgctcac agaggcaggg cttgaagtgg tcagggtgat gcgaagcagt atcagcattg
     1501 cagacccgaa cggtgggaag aatatcaggc tgaaaggagc attttatgag caatctttta
     1561 cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagacaatg
     1621 ctgaacgacg agttcagcaa gctcgaaaaa tctgtaagca aggctgtgac atcaaacgag
     1681 acgaaaatca aagacgctat agccctgttc acagcttcga cagaggaatc actgaaaaaa
     1741 caccgggaag gggtgaaaga ggcgatgatg cagcacagga aggacgtgtt aaagctggca
     1801 gggaatacgg gcatgatgtt actggggatg gtccttttcc tgtttaccgt gagtggcggg
     1861 acgctctggt atcttggcgg gatgatacag gcgaacctgg aagaaatcag gatacaggaa
     1921 gaaacattgc agaaactgaa cgcgaagaca tggggcgtgg agtttgtgca ggacgggaga
     1981 aggaaatttc tggttatccc tcaggggaaa tcggcaacgg tgattcccta tcaggggaag
     2041 gactgggtac aactgacgga gtgacacaga gtgacggagc tggaaagaca tttgctgaac
     2101 gccttagagc aactgcaaca ggactatatg cagcggctga acgaatggga gagcgccttc
     2161 gtggaattgc agaaaatgtt ttcgcttacg caacgggaca acgcgatgct gaacgagcgg
     2221 gtcatgcagt tgagtcagca ggtgcagcac ttgagcgagc agacagaacg cttgagccag
     2281 ttatacagcg agaacaggag ataagggatg aacggctgat acaggaacgc gaacatgagt
     2341 tatccctgga gcgtgagcgc cagctggaaa tacaggaacg gacacaggat ggcccttcgc
     2401 tgggatggta atcagagaca ggctaaaatg ttgtcagcgc gtttccggtg cattttttgg
     2461 gataatggtt ggttcggggg caccgggaac gcgcttcagt cagaccttgc tgaaaacaca
     2521 ggaacgcgtt aaaacgcata cgggcgcgat tctggtgtgt cttttgttgc tgtgttttgc
     2581 ctctattgcc agatcgcgct cattcccctg aacttctgtt ttcccgctcc ggtccccgtc
     2641 ttcttcgctt ccgaaagcat gaaaaaaatc cgtttcagct gtctctggct ggcaattcct
     2701 gcgtgattca catggctgca tagctatgca tgtggttaaa atttaccggg tgcgatcgcg
     2761 gcagtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat cgcgccgaac
     2821 gcgtttcagc ggtgcgtaca ataagggatt atggtaaatt atcttgttcc ttagagaatg
     2881 atttgaggtg gtatcctgca cttatttaaa tatgcttgct tgggaaattc caggttgcag
     2941 tatggttgag ttgtaatttt cctatgatag gaaaaatgtt atattattaa tttatttgtg
     3001 aggcttcata aagaatgaaa atttctgata ttgaaaaaaa catatataaa ggactagctg
     3061 aatttatttc acaaaaaatc agagatgaac atgatatatg ccttggacag tttgaatcag
     3121 aggaattact tgatgagatt cttaaaagag taatcccagt catatataat gtggctattg
     3181 atgatgctat aaaatcagta aggaatgttg gggatagatt agaggaggaa ttagatatga
     3241 ggaaggtagt gtgactaatt ttaaatcaga cttaaatcta ataagaaaag aatggtataa
     3301 tgcattttat tcaggtaata ctgaaatgct agattacttg gaagctgatt ggtttatttc
     3361 aacaaatggt caaaaagtta tctataaaaa acatcagtta cgaaagattg atatgcgtcc
     3421 agcgaggatt ttacctagca tagaaagata tgaatatgat gtcgtaatgc gtgagtttaa
     3481 aggcattgct tgtgtttcag ggaaagctga aataaagcaa aatgatggaa atatattaat
     3541 ggcttttatt gagaattgga taaaggttaa tggtggatgg aaaatgcagt ttatatcgta
     3601 tgaaaaaatc taaggggaag cccccttaga ttaatggtta tatatgttca agttttattt
     3661 ttttatagcg ataagttctg ctttgagctt taagcatgtt ttctgcaaac tgaatatcaa
     3721 aactatttat agcattagtt attatagttt ttattttttc tttatcagat aatgaatcac
     3781 catgctctat aggttcccgc atatatggat tgttttttaa ttcatcaaat aatgttttaa
     3841 atagttctaa tcgacgaggt cttataactg taaaagatat atgaagagag tgtttgctct
     3901 gagtcgtaac attgtgccag tatccagcag gaagatatat ggcctgacct ggggttatat
     3961 caaactcata atctggggag gtattaggtg gtagataatc gaagcttctt tcatcttcga
     4021 gagggtatga ataggtaggg ttgtaaactt tccatttttt tattccctca atttgaacag
     4081 ctattacatc atgattgtca aaatgaagcc caaagcttgg tgttgctgta aatgcagcat
     4141 atagattagc actacatgta cattctaact ctttagataa ccatagtctg ctttcatcta
     4201 cagattcaaa gaatgactgg catcgatcta ctataagggt tgcgccatca cgtaagcatt
     4261 tatatagctg atggcgattt atatgtggtg ttctatcgcc tgtttggcta tatgtgtatc
     4321 ttataaatcc tttatacccc ctaatttcgg gaaagttgtc atttgccatt ctaactctag
     4381 gatagtgcaa tatatctttt tcaagcaatt tattaagatc attccagttg attaagtttt
     4441 tataattaac atccgtgaat acaaacggat atttctggaa attatatttc ttaaattttt
     4501 ctatgatttc cttttttata agattattca tttttaaacc tcatgttttg tgatatctat
     4561 aatctgtgct ttaggtatat tatttataag ttgttcatca tgagatataa caatgatatg
     4621 cttttccttg ttttcctctt ttatataatc actgattatt tttataccaa gtgaatcaaa
     4681 cccttttgtc ggttcatcga aaataatcaa gtctacgttt tttccagatg taagagatac
     4741 tcctaatttt tgtttttgtc caccagataa attatgtccg taacctttga tctcataatc
     4801 tagtggtttg tcgaacttaa atctgtgtag cagcttttta attgtattta aattattaat
     4861 gccggaataa tgtaatatat tttcctctat agttccaggc atgagatcta gatcttgagg
     4921 aacgtataag acattaacaa aaggagaatt tgctttttct tgttgtatcc cattgatttt
     4981 tattgtgttt tcttctgttg ttatattgcc agagagaatt tttgcaatag ttgtttttcc
     5041 cgaaccattc tcaccggtta taaaggtaac aacgcctttt gttaatatta atttatggtt
     5101 gaatgatttg ttgtatattt caatattgca aatattgtct tttacatgaa gatattgctt
     5161 gtctgtgtaa tttgtttttt ttatgtcgtt tatttcggca cagtataaat caatttctgt
     5221 tcttgccatt tttatttgtc taaatagata accaatgcga tttaatggga gaaaaacttg
     5281 gaataaaaaa gtggcaagca taataatact accacggctt gctctaccat gaagaaacat
     5341 ccagtatgct gataggataa tcagactact aaaaatgact gaaagaaaag cggagctgat
     5401 accaaacagg aaaaattcat tatttacttt tctttgtttt tctgaatgtg tatttacagc
     5461 ttgaattact cgtttacgac ttttatccgt ggtatggaaa gctttatcca taggatacat
     5521 gtgcatcata tcaaacattc ttccagatgc gaatttggag ctaagtgcaa ctgatttcat
     5581 cattggaaat ctacgttgtg tcatttttat gacaaatatt atatagattg ccattattat
     5641 tataaactca atggctatta atgtattaac acttatacaa attgttatga ttattccaat
     5701 catttgtatt aataatggta ctacatcaga acatattgta taaaataatg atgatgcaga
     5761 agaaagcatg ctttctataa ccttatttag ttctccttta tttttcttta aaaaatgctc
     5821 ttggtgagaa ttttgtatat tatcaataac ttgctctcta agctttacag ataattctat
     5881 atttgttttt tgagtcaagt attccgatat gctatatcca gcaggcataa ggaatctaat
     5941 tgataagaaa catgctataa atattaataa tgagtttatt tctcctattg aatctttatt
     6001 gtcaataact gtttttatta aatatccgaa tagaacgaaa agaaaactac ttatagttga
     6061 taataatata ataaaaaaca aattacattt attaaatgtg atgatgtact tttcaataaa
     6121 attcaattta taatccttaa agcccgtata ttacgggctt tatgttatct agaattacat
     6181 gagcacccag cgccacatcc tttaccgctg caaccacaaa cagaaccatt atcattccaa
     6241 acatcttttc gcattattat ttttacaaat tctgcggctg gaaggtgagt gatactcttt
     6301 actttttcga tgtaagaatc actggatatg gattcttttg gaattcttat agtattaatg
     6361 ttctttatgc tgtatcttac agcataatca ccataccact ttgcgacagt ttcatgacct
     6421 aggtaaaaag cgattttttc aatttgattt ttatattcgt taaaacgata ttcttggaat
     6481 gttataccat gctcattaac atgccttatc tcatttttta gcatgtcatt tgtatttgga
     6541 taatggtgga ggtaagcacc aagatatttt tcacaaaagc tggtataagc atctgtaaat
     6601 tgaataaacg tatgccacat ttcatctata atttgtaatt gctcgtgaac ttttatattg
     6661 tagcattccg ttaggcatgt tgaggctagc cagatgaatt tctttgtttc attaaagata
     6721 tcatcagctt cttctggtgt tacattccat tctttacgaa attttagaag aacatcatca
     6781 ttgtgataac tcatgatatc ttctaatgag ctaacattat gaacttttgc ataagaactg
     6841 ctgttcatct tatgttgtag cataacaccc ccaattatat atttgaatgt agatgagaat
     6901 agttatcatt tgttatggta ttgttaaatt tgtgcttgtg tcaatatgcc taattctata
     6961 aatgagatag taatcaagtt ttttaatggt tataggatcc attagtaata caaggttttt
     7021 gcagccagtt tttacgggta ggggctgcaa ggaaaacaaa attttcccac tctaagagaa
     7081 gagaattgag ttggttgaat gagtggatgc atctagggcc gctaattcac cgagtgtaaa
     7141 ggctgtacga aatgtgttgc agtccgataa tagccccctc cattgacgtt ttacacatgt
     7201 gttgtaggtt ccaggagtgg gcggatctca aaaagatcaa aggatcttct tgagatcctt
     7261 tttttctgcg cgtaatctgc tgccagtaaa cgaaaaaacc gcctggggag gcggtttagt
     7321 cgaaggttaa gtcagttggg gactgcttaa ccgggtaact ggcttggtgg agcacagata
     7381 ccaaatactg tccttctagt gtagccgcag ttaggccacc acttcaagaa ctcttaatat
     7441 ctcaatccac cttgtccagt taccagtggc tgctgccagt ggcgctttgt cgtgtcttac
     7501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gagccgagat acctacagcg
     7621 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttat gatgctcgtc
     7801 aggggggcgg agcctatgga aaaacgccaa cggcgcagcc ttttcctggt tctcgttttt
     7861 tgctcacatg tttcttttgg cgttatcccc tgattctgtg gataaccgca tctccgcttt
     7921 tgagtgagca gacaccgc
//
