LOCUS       NZ_CP119124             9763 bp    DNA     circular CON 05-MAY-2024
DEFINITION  Escherichia coli strain GENOMIC22-004 plasmid pCFSAN126949_04,
            complete sequence.
ACCESSION   NZ_CP119124
VERSION     NZ_CP119124.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33426038
            Assembly: GCF_029094485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Hoffmann,M., Luo,Y., Sorensen,L.H., Pedersen,S.K. and
            Hendriksen,R.S.
  TITLE     Results of the 2020 Genomic Proficiency Test for the network of
            European Union Reference Laboratory for Antimicrobial Resistance
            assessing whole genome sequencing capacities
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   Hoffmann,M., Luo,Y., Sorensen,L.H., Pedersen,S.K. and
            Hendriksen,R.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2023) CFSAN, US FDA, 5001 Campus Drive, College
            Park, MD 20740, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbial Genome Analysis v. SMRT Link
            11.0.0.146107
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Pacbio Sequel IIe system
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029094485.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 06:49:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,128
            CDSs (total)                      :: 5,008
            Genes (coding)                    :: 4,729
            CDSs (with protein)               :: 4,729
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 279
            CDSs (without protein)            :: 279
            Pseudo Genes (ambiguous residues) :: 0 of 279
            Pseudo Genes (frameshifted)       :: 97 of 279
            Pseudo Genes (incomplete)         :: 178 of 279
            Pseudo Genes (internal stop)      :: 45 of 279
            Pseudo Genes (multiple problems)  :: 40 of 279
            Pseudo Genes (short protein)      :: 4 of 279
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119124.1.
            Bacteria and source DNA available from Sorensen, Lauge Holm,
            lahoso@food.dtu.dk.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GENOMIC22-004"
                     /isolation_source="EQA test strain"
                     /db_xref="taxon:562"
                     /plasmid="pCFSAN126949_04"
                     /geo_loc_name="Denmark"
                     /collection_date="2022"
                     /collected_by="DTU National Food Institute"
     gene            join(9188..9763,1..51)
                     /locus_tag="PYZ88_RS25240"
                     /old_locus_tag="PYZ88_25200"
     CDS             join(9188..9763,1..51)
                     /locus_tag="PYZ88_RS25240"
                     /old_locus_tag="PYZ88_25200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="WP_275289566.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGADGGMTTGVGLMYQDH"
                     /name="TonB-dependent receptor plug domain-containing
                     protein"
     gene            84..521
                     /locus_tag="PYZ88_RS25245"
                     /old_locus_tag="PYZ88_25205"
     CDS             84..521
                     /locus_tag="PYZ88_RS25245"
                     /old_locus_tag="PYZ88_25205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_275289567.1"
                     /translation="MDGENSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYN
                     NHWWYDLPISGTGHHLNLPRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQN
                     WRNADLLGTATYRHKDTRQSMYQQVWGSLREYNSKSKYGDH"
                     /name="hypothetical protein"
     gene            540..1649
                     /locus_tag="PYZ88_RS25250"
                     /old_locus_tag="PYZ88_25210"
     CDS             540..1649
                     /locus_tag="PYZ88_RS25250"
                     /old_locus_tag="PYZ88_25210"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="WP_275289568.1"
                     /translation="MSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYIENVNIF
                     DHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYTSKGTSI
                     NTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGKNYETGI
                     KGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGIDFELQG
                     MVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGKLTNWRA
                     GVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITNVFDKVY
                     YTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent receptor"
     gene            3233..5194
                     /locus_tag="PYZ88_RS25255"
                     /old_locus_tag="PYZ88_25215"
     CDS             3233..5194
                     /locus_tag="PYZ88_RS25255"
                     /old_locus_tag="PYZ88_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5194..5925
                     /gene="mobC"
                     /locus_tag="PYZ88_RS25260"
                     /old_locus_tag="PYZ88_25220"
     CDS             5194..5925
                     /gene="mobC"
                     /locus_tag="PYZ88_RS25260"
                     /old_locus_tag="PYZ88_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5932..6462
                     /locus_tag="PYZ88_RS25265"
                     /old_locus_tag="PYZ88_25225"
     CDS             5932..6462
                     /locus_tag="PYZ88_RS25265"
                     /old_locus_tag="PYZ88_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6490..6669)
                     /locus_tag="PYZ88_RS25270"
                     /old_locus_tag="PYZ88_25230"
     CDS             complement(6490..6669)
                     /locus_tag="PYZ88_RS25270"
                     /old_locus_tag="PYZ88_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6738..7115
                     /locus_tag="PYZ88_RS25275"
                     /old_locus_tag="PYZ88_25235"
     CDS             6738..7115
                     /locus_tag="PYZ88_RS25275"
                     /old_locus_tag="PYZ88_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(7177..7476)
                     /locus_tag="PYZ88_RS25280"
                     /old_locus_tag="PYZ88_25240"
     CDS             complement(7177..7476)
                     /locus_tag="PYZ88_RS25280"
                     /old_locus_tag="PYZ88_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7466..7729)
                     /locus_tag="PYZ88_RS25285"
                     /old_locus_tag="PYZ88_25245"
     CDS             complement(7466..7729)
                     /locus_tag="PYZ88_RS25285"
                     /old_locus_tag="PYZ88_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            8106..9191
                     /locus_tag="PYZ88_RS25290"
                     /old_locus_tag="PYZ88_25250"
     CDS             8106..9191
                     /locus_tag="PYZ88_RS25290"
                     /old_locus_tag="PYZ88_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     Typing          complement(1855..2435)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5195..5922
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 gcggacggtg gaatgactac cggggtgggg ttgatgtatc aggaccactg aatgacagcg
       61 cgaccttacg cggaagggca gttgtggatg gcgaaaacag tgagaccttc agggatagtg
      121 agaaacgcag gaataacgtc atttacggaa ttatggaggc agatctgact gataataccc
      181 tacttacgat tgggggatat cagcagtatg aatataacaa tcattggtgg tatgacttac
      241 ctatttctgg taccggacac caccttaatc ttcctcgctc gacgtttaca ggcaatgact
      301 gggagtttga aagaagtaaa agtaaaactg catttgcaac tcttgagcag aagttcagta
      361 atagctggaa gctgaaattt tcaacgctgc aaaactggag aaatgctgac ctgttaggga
      421 cggcaacata ccggcataaa gatacccgac aaagtatgta tcagcaagta tggggatcac
      481 tgagggaata taattccaaa agcaagtatg gggatcactg aggcgaatat aattatgacc
      541 tgtcgcttaa tggggactat gctttatttg gtcgtgttca tcaattcgtg gctggaacaa
      601 ctcagcaaaa atacaccgat aaagatacag gttatacata ttccccaata tatattgaga
      661 atgttaatat atttgatcat ggttcacatt cttcgaaaaa acccatgaag atgtcaggtg
      721 atgcatccta cgacgtcact gaacaaaaaa gcgcctacgc agcaactcat tttctactta
      781 gtgataactg gaaaatattg ctgggtggcc gtcttgactg gtatgaatat acaagtaaag
      841 gtacatccat taatacaggt tataaagtta ccagaaatat caccaaatat gcaggttcaa
      901 catacaatct tgacgatcat cataccattt atgggagcta taccgatata ttcaatccac
      961 agtcaagcac cgacctcaat ggtaaagtga tcaagcctat tgttggtaag aattatgaga
     1021 caggtattaa gggtgagtat tttaacggct tgcttaatgc ctctctggcg gtatttcagg
     1081 tagaccagga aaatcgagcc aggctgcttc ccgatcagac agggtgtgtt actaatggca
     1141 atcctcaatg ctatgaggct agcggtaaag tacgcacaaa agggatagat ttcgaacttc
     1201 agggcatggt tacgccactc tgggaaattg gggtgggata tacatatgca gatacgcgat
     1261 atataaaaga tggagatcgt agtaatgaag gcaagcgatt caatactgcc gtgtcgccac
     1321 aaaatttatt taagatttcg agcatgtaca cctttgacgg aaaattaaca aactggcgag
     1381 caggagtaaa cacgtactgg caaagtaaaa tatatgctaa cataacagac acaagggatg
     1441 gcaatccggt tattaataaa caaaaggctt attcaacaac tgacattatt attggctata
     1501 ctccaatgcc acaactggat ttacagctca atataactaa tgtctttgat aaggtttatt
     1561 atacatccat aggtgaatcg attggatatg gcacggaaac atatggagaa ccaagaaatg
     1621 ttaaaattag tgcaacctat agattctaac catgcggatt gggtcttctc ctcggatttg
     1681 cccctataaa tccgtctttt gtggttccgg cgtacaaccc cctgtttgcc acttcagttg
     1741 atggttaatc aaatcatgta gaggatcttc ttgagatcct ccatggttcg tcgtaatctt
     1801 ttgccctgta aacgaaaaaa ccacctgggg aggtggtttg atcgaaggtt aagtcagttg
     1861 gggaactgct taaccgtggt aactggcttt cgcagagcac agcaaccaaa tctgtccttc
     1921 cagtgtagcc ggactttggc gcacacttca agagcaaccg cgtgtttagc taaacaaatc
     1981 ctctgcgaac tcccagttac caatggctgc tgccagtggc gtttttgcgt gtctttccgg
     2041 gttggactca agtgaacagt taccggataa ggcgcagcag tcgggctgaa cggggagttc
     2101 ttgcttacag cccagcttgg agcgaacgac ctacaccgag ccgagatacc agtgtgtgag
     2161 ctatgagaaa gcgccacact tcccgtaagg gagaaaggcg gaacaggtat ccggtaaacg
     2221 gcagggtcgg aacaggagag cgcaagaggg agcgacccgc cggaaacggt ggggatcttt
     2281 aagtcctgtc gggtttcgcc cgtactgtca gattcatggt tgagcctcac ggctcccaca
     2341 gatgcaccga aaaagcgtct gtttatgtga attctggcag gagggcgaag cctatggaaa
     2401 aacgccaccg gcgcggccct gccgttttgc ctcacatgtt agtcccctgc ttatccacgg
     2461 aatctgtggg taactttgcg agagttagca gcgcccgccg cagtctcacg gccggagcgt
     2521 agcgactgag tgaggggagg cgcaatatcc cccctcccgc agacggccac cggacgcgcg
     2581 gatgtgacat attgcaccgt gttaccgcct ttcccttccc tgccgataaa tcattcagat
     2641 aggtgaagtc tgtcggtttt ctgctgaata ttttacgtta acctatctct gttgcttttt
     2701 tctccttgcg gagaaatgca ggtcatcccc gctggggcgt ggctgacttc aggaggaaat
     2761 cccctgctta cgctggctgg acgccaccga cgcacggtct ttccccctct ttctggctca
     2821 gctcggctgg acgccggcca cgccagaaat tcacccggcc cccgtcaggc tcacgcctga
     2881 ccacccacaa aaacccacgg tgctgaacgc acctgacgaa gcagcaaagc tgctcctgat
     2941 tttgcgggtg gtcagccgct cgctatggcg aactctgacg ggggccggaa caccggaaaa
     3001 gaccgcactg gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt tgttcctccg
     3061 gggctgcgcc ttttccgctt ggggcggaaa gaggctggcc tccgtggtga aagctgaatg
     3121 caatcaccac ggacgtgacc gccgccgcca tgcctcgggg catgtccgcg gcttgtgcag
     3181 caggggctgc acaccacgcc ggtaaacccg tcgtctgaag gagagagatt gcatgtttaa
     3241 tacggattcg cttgcctggc agtggcatat cccggagctg gcccggcaga tttacccgtg
     3301 ggtcagctgg ctgtacaggg agtattacgg tctggccggg gctttcctgg ttatcggcct
     3361 gtttttcggt ctggctgaac tctgggccgg acggatgcgt tttgtggggc tgagtcataa
     3421 ggagcgacgg gagtatgccg atcgctataa gcgcgataat aaccggactg cgccattttc
     3481 ggtgctggct tcgctggatt cgcccgggcg gatggtcagg gcacggcggc atgtctggaa
     3541 ctacgcgtac tggctgaccg gcgctgccgg tctggccctt ctgggggggc tggttgtcgc
     3601 cgtgtttatc gagagctggg gagaaaccgt cctgcgtccg gacctctacg cgaacgcaga
     3661 ttattacctg ctgcagccgg ctaacctgtt cgggattgtc tggtgtgccg gagggcttgt
     3721 cgccggcatc cttctggcgc ggctgctggc atttctgctg cttgacggtc attttgcggc
     3781 cgctgacgag gcggtgaatg cgaaactgaa ccaggagagc cggcgcagca gccagcggac
     3841 cggagaaatg accgacgtcc gccacctgca tttcggggag ccggtaccgg ttaacgcgct
     3901 ggccgatttt tccacagagc aggcccggaa acagcaggcg gtgtttctgg gtaaggatga
     3961 gcagggccag ccggtgctgg tcccgcgtga tacctggcgc aagaccaata ttcagatcct
     4021 cggcctgccc gggagcggca agagcgtgat gggcaccaac gcgctcattc gctgcgtacg
     4081 cgatttcggg gacgcggtgg tgtacttcga ccccaaaggg gacgcgtggg cgccgcacgt
     4141 cttccgggcg cactgcccgg acttcaccct gctggatctg cggccgggga aaccggcgca
     4201 gctgaatctg tttcgcgatc tcgaccagta cgccctgaag aatctgctgg ttgccgggtt
     4261 taacctgagt gagaccggcg acgtggcgga ccactaccgc atcagcgagc agaaagcggc
     4321 gaagctgatt gcggagcagt tcccgcaggg ggcaaacatt cagcaggtgc tggcggcggc
     4381 atacgcgctg ccggaggccc tgaaaaagga cgtgaagggg ctgatcacca agctggagaa
     4441 cgtggccgac ctgagcgtcc tgcagacgga cagcggcatc gacgtggcgg ggataatcaa
     4501 cggcggcggc tgcctgtacg tcatcggctc gatggacgat gaggcggtga tccgggtgca
     4561 gaagatgctg tttgcccgct gcgcgcaaat catcattgcg cgggatgagt tccggaggtg
     4621 gccgcacgcg agcatcatgc tcgatgagat taaatacctg ctgtcgaagt acgtgctgaa
     4681 cgcgctgggg acgctgcgca gtcgggactg caacctgctg ctcgcgcacc agtcgctggg
     4741 cgacttcggg cagtgcggcc aggatctgcc cgctgagttt gtgaaaacca cggtgctgga
     4801 taacacgccg atccgctggt tctaccgggc ggccagccag gagtccgcgc agtgggcggc
     4861 gggccagacc ggggaaatcc gggtggacgt ggagcgccgc cgggccagcc gggaggcggg
     4921 gaacgtggag catatcagcg gggacagctt cattcagaag gaggcgcggc cgctgtttga
     4981 cgtgaacacc ctgcagcacc tgcctgacgg gtttgcggtg atgaccgggc tgggcgtggc
     5041 ccggatggcg ttcagcagcc cgctgcgggt tgaacgccgg gaaatcccgc tgcagcgctt
     5101 cccggtgctg gcgaaaacgg acccgctggc ggagtaccag ccggaggccg ggcggcagcg
     5161 gccgggtgat gatgattttg cggggctgta ctgatggcac tggagcgata caacgtcagc
     5221 cacgcgaagc ggcaggcgcg gaacgcggag aaaacccggc tgaccctgcg ctggctgcgg
     5281 gaagagctgt gttccacggc ggagctggtt gcgcgacggc tggggattgc ggcggtgcag
     5341 ccggtttacc gcttcctgga cagcctggtg gcgaaagggc tgctggtccg ggcgaaatac
     5401 ccggttgacg gccgtcaggt cagcgtgtgg ggactgacgc cgcacggggt ggcgttctcg
     5461 ttcgacgagg acgaaccgct gacggacgtt attccgtttc agccgtcaag ggtgtcggcg
     5521 gcgcagctgc cgcaccggct ggccgtgcag tccctgcgtc tcgccatgga ggcccggggg
     5581 gcctccggct ggcggtacct gcaccgtatg gccctgaagg ggatgaaggt gccggacgcg
     5641 ctggcggagc tggacggcag aacggtggcg tttgaggtgg agcgcaccgt gaagagccgc
     5701 aggcgctatc aggaggtggt gtccgggtat ctgttcaatc gcaaagcgaa cggcatcgat
     5761 gaaatctggt acatctgccc ggaccgggcg acgcaggtca gggtgcagcg ggcgatcctg
     5821 tcggtggatg aaatcgtgaa tccacagacc ggcgaggccc gcaaaacggc ggagctggac
     5881 cgggagcggc tgtttgcgtg ctttaaattt atgacaacgg agtgagggat tatggcgctg
     5941 tacatggtat ttattgaaga cgcggaggac cgggacggcg agcgctgcat catgccgacc
     6001 agcgagcatc ttgaagatat ggtggtgctc gcctggcaga ccggggcgta tgttttctgt
     6061 tttgtcgacc gggcccagca tctggcgatg atgaaatcca tcgggatgta tgacgagcgg
     6121 ctcaacagcg acgaaaaagc cacgtacttt ttcaccggta aaaatccgca atgggaacgg
     6181 gacaaacccg caggaatgaa gctgtgtgat tacctctatc gcaaggtgga gcggttcgcc
     6241 atcgagaacg gtgtgcaggt ggttgatgcc gggaagtgct caccggagca gattagcgcg
     6301 atgattgagg tggtcagcga gcggcacggg gaaagcgcgg tgaacgagaa gctgaaagcg
     6361 gagctgatgc gggaatataa cagccgctat tttaacgtgc cggtgcctga actgccggag
     6421 gacaaacccg cccgccggtg gtggccgttc ggcaaaaaat aaacggcagc accggccatg
     6481 ccggtgctgt tattccttcc cgagcttatc ccgcatcacc tgccagagtg tttccgcatc
     6541 ctcgtgcagc cgctcgcaca tgtcggccgg ggtatccata tccagctcgt tcagtttctc
     6601 gagtaacagc agcgactggt ccttaataaa tttagccata ttgagggcag ttttttcctg
     6661 cttagtcatg ttttctgtcc ttccgggtgt ggtgtgcttc ctgtcgggta tactgtctca
     6721 aaaccggagg tgagacaatg gcgtgggtga cggtgagaca ggcgacggaa ctgacgggga
     6781 aagcccggag ttccctgtat agggatatgg cgaaaggccg cgtatcatac cgtactgaag
     6841 ctgatggtgg tcgggtggtg gataccagcg aacttatccg ggtgtacggt gaattaagac
     6901 agattgagac tcacgagcgg gacgggttga gactgtcgga tgagactgaa aaaatggcct
     6961 ccgacgcgtt gatcgcggag ataaaagcac tcagggagga ggtggccgga ctgcggcagg
     7021 aaatgcaggc catgagactg ctggaacaca aaccagagac agcagagaca ccggccggaa
     7081 catcgcgccg atggtggcag tggggaaaac gctgacgcgc tgtgcttgtc caaccggcga
     7141 cggccacgca atgaattgcg cccccatcac cggttatcag cgaacggtat ggggattctg
     7201 gcgctggcca accacggcca gaacccggac tttcctggtc tggtcatcaa catcatagag
     7261 gacatgctgt cccatcagac tgatccggcg aacgtcctcg ccatagcgtg agtccacttc
     7321 atataggcgc gggaagtcgg ccagtttgtc cgctgcagcg agaactcgat cggtaacgtt
     7381 ctgcgcatcc cacagggatg aattttccgc aatgtagtca aacaatgctg tcacatcctc
     7441 gatggctccc tgcttccatt ccacctcata cggcatcgcg tagttcctga tagcggccat
     7501 gcagtctctg gcggagttgt tccgtggtca tgcccgggcg cgtgtcagcg cgggcggcgg
     7561 tcactttagc gcgaacctga tcttcatatt ctgcctgggc cagttcttcg ttagccaggc
     7621 gctgcaacag tgcagctttg tccgggccac cttcaggcag tatgcggggc agtctctcgg
     7681 ccagttcccg caggaaattg gcgttttcat agtgtgtact gttgctcatt gtggagcctc
     7741 ccgggctgat acgtcgatat gaaggagttt agcagggggc ggtctgaaag aatatgctcg
     7801 tgtcagtcag gctgatgaag acgaaagctg gtaaaaaagg gcttacgaca cccattttac
     7861 gtcaaactgc acgacatgca gttgcgtgca tagccatgca gcgggttaaa atttatcagg
     7921 cgcgtttccg gcggttttcc gggtggtttg ttgccggttt tatcccgtag ccgccggaaa
     7981 tgccctcaga ccgtctgagc ggtgcgcgta atgcgacgtt atggtaaatc atgtgattaa
     8041 tggtagtgat aatcattatc acttttgcta tattcttgtt tcttttattg taaggaaatg
     8101 taagtatggc aatcacgatt aaatccgccg ttaatgtccg tagcagagta ttaatatact
     8161 ctttactact gtttactggt tcagcgtatg cggctgatat agctgaatca acattcctga
     8221 ctcgtgatat cggcaatggt ctgtatgaaa tgtcttttga tggcaaaaat aatagtctct
     8281 gggcagcatc atcgtcttca ttcgacagag ataagggcca cggaattgtt tatcagttag
     8341 aaccatccac tctgaaaaca aaatctgccg ttaaaatcgc acgtcttggg tttgccacag
     8401 cactggatga ggaacatgag cgactttata tcggcaatac tctggacggc tcagtgacgg
     8461 tcattgatac tctaaccgga aaagaacttt cagtaattcc gttaactgag ggacaggatg
     8521 aggctcattt cagaccgacc agagaaatgg tcattgacaa aaaaaatcag cgtctttatg
     8581 tctccagcgc cgcagataaa ggggctctgt gggttattga catggctgtc gggaagaaaa
     8641 ttaaaaccat tgatggactg gggcacgctg ttactggaat tgcgttaaat aaaaatgggg
     8701 ataatattta tctggttaac ggggatggtg agattattaa cctggatgcc agtagctatg
     8761 aaataaaaaa acggtttgtt gtagagcctg agaaaaaaca ctttttcctt aatgtaagtc
     8821 tggatgaaaa aaacggacgt gcatttatta ccgacccgga tattcccggg gtgctggttg
     8881 ttgatgtaaa ctcgggaaaa atactacaca gaattgatgt tataaattca ctggcgattt
     8941 tatttaatcc tttacggaac gaaatctata tcacgcaccg taatgcaaaa caggtcagca
     9001 ttgtggacag taattcttat tacgtaaaaa caacgataaa aacccgcctg atgccaaaca
     9061 gtctgtcttt atcacatgac ggtgaaaatc tgtacgttag tgtaaaacaa gaaaaaaaag
     9121 agatgagtac tatgaaggat tacatactca aaatcgaatt atctaaaatt gctatggagc
     9181 cagaagaatg aataaatatt tagggctgag catagtatat atgatgtata catcatctgt
     9241 tatggctgat gattctggcg cagttagtaa agataaaaat atttcaccag aagaaacatt
     9301 aattgttcat gggaccgctg tttctgaaag cacaacagaa ggaacagatt catacacaac
     9361 ccagtcagcc aatacagcat caaagatgaa tctgtcattc cgggatacac ctcagtcaac
     9421 tacagtcatt acgcgacgaa gaatggatga tgtagcagca aaaaatatta ttgatgtggt
     9481 taaaaccgct cctggattat atgtcagtac agcagaaggt tccggaagac cgaattttat
     9541 gtccagagga tttaatgcta atgtcatgta tgaaggattt agcctgccgt ggaattcata
     9601 cattccaagt tcacaaacta gttcagctat ttttgaccgg gtggagatag tccgcggggc
     9661 aacaggtctt gttcagggtg ccggagaccc ttcagccgcg ataaatatga tacacaagcg
     9721 tccgacccgg caatatcagg ccagcactaa catcggtgcc gga
//
