LOCUS       NZ_CP119010             2590 bp    DNA     linear   CON 14-JUL-2023
DEFINITION  Escherichia coli strain CE0011b plasmid pCE0011b2.
ACCESSION   NZ_CP119010
VERSION     NZ_CP119010.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33425307
            Assembly: GCF_030419105.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Burgess,S.A., Mwenifumbo,M., Cookson,A., Benschop,J. and Fayaz,A.
  TITLE     Whole genome sequencing of antimicrobial resistant E. coli isolated
            from dairy calves
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Burgess,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2023) School of Veterinary Science, Massey
            University, Tennent Drive, Palmerston North 4474, New Zealand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2022
            Assembly Method                   :: Unicycler v. 0.4.4
            Assembly Name                     :: CE0011b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/14/2023 11:44:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,076
            CDSs (total)                      :: 4,953
            Genes (coding)                    :: 4,723
            CDSs (with protein)               :: 4,723
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 88 of 230
            Pseudo Genes (incomplete)         :: 149 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 60 of 230
            Pseudo Genes (short protein)      :: 4 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CE0011b"
                     /isolation_source="feces"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /plasmid="pCE0011b2"
                     /geo_loc_name="New Zealand"
                     /collection_date="2014-09-15"
     gene            551..1540
                     /locus_tag="PY721_RS25380"
                     /old_locus_tag="PY721_25380"
     CDS             551..1540
                     /locus_tag="PY721_RS25380"
                     /old_locus_tag="PY721_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_301221568.1"
                     /translation="MQNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYEHYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKAESD"
                     /name="protein rep"
     Typing          1544..1627
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.941"
                     /coverage="93.0"
                     /evalue="1.22e-26"
     Typing          1753..2008
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.574"
                     /coverage="89.0"
                     /evalue="2.65e-108"
     Typing          complement(2488..2569)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="93.0"
                     /evalue="3.4000000000000004e-27"
     Typing          complement(1267..1458)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.446"
                     /coverage="100.0"
                     /evalue="2.86e-91"
ORIGIN
        1 aaaaaattcc gcgtaccgct catcgttccc gaactcgtca aacagaaaat cgcggggccc
       61 cgcgcctctg gcgtgggttc cgattttgtg attgacgacg gcacggggaa aacactgggg
      121 atggtggttg gtgtcacgga gtgaggccaa ccacctgcaa gacggttttt tgttgttcac
      181 aaaatcgggg tgaaagtctg tggatgagat gggtgtaagt gggtggggtt gggaggtttt
      241 tggttgctga aaatccagct caaaattcaa gcaaatctcg cgcgtgatcc ttgtatttat
      301 acttaaggga taactctagg attcaaattc gcctccagcc cttgtgccgc aaggcctgag
      361 gggggatttg ccggaaaatg gttttggagt tattctgtcg ggagtggttg cgttaaaagt
      421 gacccccgca acgacgggca tcgttcgggg gtgtgaccac ctgcagaaac tggattcatg
      481 atgagagcaa caggtgatat ggcaatgata acacgtcaat cgagccatcg cccagtatgg
      541 ggcggtgtgc atgcaaaacc ctaaactcca gtacctcact gattattccc cctctgatgc
      601 gccctgggac gcacacagaa gcgtttctga cgacgtggga gggatttacc tgcttgcggc
      661 cgaatatgag cactacgggg ctcgtatggc ttcctgtggc ggtttactgc gtttcgggtg
      721 gtctaccctc aaggaaaccg gagaaacgcg tctgaggctg cgtgaggcac atttctgccg
      781 tgtccggcac tgtcctgtct gccagtggag gcgctccctg atgtggcaag cccgtttcta
      841 ccagtccctg ccgcgtatcg tggcggatta tcccgatgcc cgctggatgt tcctgaccct
      901 gaccgtgcgc aactgtgcga ttggggaact gggcgagatg ctgaaccgga tgaatgcagc
      961 ttttcagcgt atgaaggttc gcaaggagtt cctgcctgtt cagggctgga tccgcacaac
     1021 ggaagttacc cgcggcagcg acggcagcgc acacccgcac ttccatacct tgatgatggc
     1081 tcctcctggc atgctaaatg ggaaaagtta cgtcaggcat gaacgctggg ttgagctatg
     1141 gcgggagtgc ctgcgggttg attatgaccc gaatgttgat gtccgggcag tgaaaccccg
     1201 caagccgaag gatggcgagt ccttagcctg cgcgaccgct gaactggtgc gcggcgcagt
     1261 ggccgaaacc ctgaaatatt ccacgaagcc ggctgacatg gtggccgatc ctgaatggtt
     1321 cctcgaactg actcgccaga cgcacaagcg ccgctttgtg gcgaccggcg gcgcactcaa
     1381 agatgtgctg aaactcgatc aggaaaccga cgccgacatg gtgatcggag atgatatttc
     1441 cgagggcgac gatgacggtt cacgcatcgc gttcgaatgg aaaaccgagg tcagaaagta
     1501 ccgccgctca ccgacgaaag ataaagctga atcagactag cagggtgtcg gggcgcagcc
     1561 ctgaccaggt ggtaattgtg atagcgtcgc gtgtgacggt attacaatta cacatcctgt
     1621 cctgtttcaa ctgataaata atccctgtgc agtacagccg ctgttgcata aaatttcgct
     1681 ctgcgtcgag tgtttcttaa tcaagaattc cgctgatact ccggaaaaac gcgagtctgt
     1741 tctcatgcct gaaatgccca caccccacgc aaaaacaatt ttttgctgat ttttctttat
     1801 aaatagagag ttatgacaaa ttagtttctc ttgctctctt tgtgatattt aaaaaagcgg
     1861 tgtcggcgcg gtgttgtagc tgcgccgaca ccgctttgta ggggtggtac tgactatttt
     1921 cataaaaaac ataattttat attaggggtg ctgctagcgg cgcggtgtgt ttttttacag
     1981 gacgcccctg ggggcgctgc taggggtgtc tgttcagata tgtagcaatg agtcagggat
     2041 tcacgaattg aaaatgtccg aaaatatgtc tcatgggcac tgtatgcgcc caaaccgatc
     2101 gccattcagt ttcatgcata gtcatgcaac gcgctgaaaa cgttcctgac gcatttttcc
     2161 ggtttaccct ggggaaaaca tccctttttg cggtgttcgc ggcagaatcg cgttcagcgc
     2221 gttttagcgg tgcgcgtaac aagtccttac accacgcggc ccaccccgcg gtcgttcccg
     2281 ttccgtacag ccatcgccac cgacgcgcca aattctggcg accggacagc aaatcatttt
     2341 gccgtttttg tgccgcagcc gttccgcgtg atcgacaaac tggcagtcag ggaatctcac
     2401 gaagtgcgca cttatacaaa cttcacttcg tttcgtttgt cgtgcggtct gcgacgctca
     2461 aaaaaccgag aaacggcggg aattttcggg acaggatgtg taattgtaat accgtcacac
     2521 gcgacgctat cacaattacc acctggtcag ggctgcgccc cgacaccccg ccaacgcctc
     2581 acggcccgcg
//
