LOCUS       NZ_CP119009             8401 bp    DNA     linear   CON 14-JUL-2023
DEFINITION  Escherichia coli strain CE0011b plasmid pCE0011b1.
ACCESSION   NZ_CP119009
VERSION     NZ_CP119009.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33425307
            Assembly: GCF_030419105.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Burgess,S.A., Mwenifumbo,M., Cookson,A., Benschop,J. and Fayaz,A.
  TITLE     Whole genome sequencing of antimicrobial resistant E. coli isolated
            from dairy calves
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Burgess,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2023) School of Veterinary Science, Massey
            University, Tennent Drive, Palmerston North 4474, New Zealand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2022
            Assembly Method                   :: Unicycler v. 0.4.4
            Assembly Name                     :: CE0011b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/14/2023 11:44:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,076
            CDSs (total)                      :: 4,953
            Genes (coding)                    :: 4,723
            CDSs (with protein)               :: 4,723
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 88 of 230
            Pseudo Genes (incomplete)         :: 149 of 230
            Pseudo Genes (internal stop)      :: 58 of 230
            Pseudo Genes (multiple problems)  :: 60 of 230
            Pseudo Genes (short protein)      :: 4 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP119009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CE0011b"
                     /isolation_source="feces"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /plasmid="pCE0011b1"
                     /geo_loc_name="New Zealand"
                     /collection_date="2014-09-15"
     gene            898..1713
                     /gene="sul2"
                     /locus_tag="PY721_RS25330"
                     /old_locus_tag="PY721_25330"
     ARG             899..1710
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1774..2577
                     /gene="aph(3'')-Ib"
                     /locus_tag="PY721_RS25335"
                     /old_locus_tag="PY721_25335"
     ARG             1775..2574
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2577..3413
                     /locus_tag="PY721_RS25340"
                     /old_locus_tag="PY721_25340"
     CDS             2577..3413
                     /locus_tag="PY721_RS25340"
                     /old_locus_tag="PY721_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            3719..3961
                     /locus_tag="PY721_RS25345"
                     /old_locus_tag="PY721_25345"
     CDS             3719..3961
                     /locus_tag="PY721_RS25345"
                     /old_locus_tag="PY721_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(3993..4643)
                     /gene="tetR(A)"
                     /locus_tag="PY721_RS25350"
                     /old_locus_tag="PY721_25350"
     CDS             complement(3993..4643)
                     /gene="tetR(A)"
                     /locus_tag="PY721_RS25350"
                     /old_locus_tag="PY721_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            4749..5948
                     /gene="tet(A)"
                     /locus_tag="PY721_RS25355"
                     /old_locus_tag="PY721_25355"
     ARG             4750..5945
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<5915..6103)
                     /locus_tag="PY721_RS25360"
                     /old_locus_tag="PY721_25360"
                     /pseudo=""
     CDS             complement(<5915..6103)
                     /locus_tag="PY721_RS25360"
                     /old_locus_tag="PY721_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(6147..6536)
                     /locus_tag="PY721_RS25365"
                     /old_locus_tag="PY721_25365"
     CDS             complement(6147..6536)
                     /locus_tag="PY721_RS25365"
                     /old_locus_tag="PY721_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            6754..7194
                     /locus_tag="PY721_RS25370"
                     /old_locus_tag="PY721_25370"
     CDS             6754..7194
                     /locus_tag="PY721_RS25370"
                     /old_locus_tag="PY721_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(7961..8110)
                     /locus_tag="PY721_RS25375"
                     /old_locus_tag="PY721_25375"
     CDS             complement(7961..8110)
                     /locus_tag="PY721_RS25375"
                     /old_locus_tag="PY721_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     ARG             2578..3410
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(8146..8319)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          2..447
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.949"
                     /coverage="99.0"
                     /evalue="1.5000000000000002e-168"
ORIGIN
        1 gaagacactt agcaaagaag agataaggcc acggcgaagc cgtttttcca taggctccgc
       61 ccccctgaca agcatcgcaa aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga
      121 ctataaagat cccaggcgtt tccccctggt agctccctcg tgcgctctcc tgttcctgcc
      181 tttcggttta ccggtgtcat tccgctgtta tggccgcggt ttctcattcc acgcctgaca
      241 ctcagttccg tgaaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
      301 tccgaccgct gcgccttgtc cggtaactat caacttgagt ccaacccgga aagacacgac
      361 aaaacgccac tggcagcagc cattagtaac aggagtgcac gagaagatag attaagaggt
      421 cttgaagtag aggcctgact gcggctacac tgaaagaaca gttttggtaa tggcggtctc
      481 gtgaaccctg ttaccgggtt aagcagttcc ccaactgact taaccttcga acaaaccgcc
      541 tccccaggcg gttttttcgt ttacagagca agagattacg attcattgaa aaggatctca
      601 agaagatcct ttgtagatta ctatcaatta atcttccgaa catgacacca gtcactgttg
      661 attacggtta aaccgctccg taacaggttt ccctcgtatt ggtgacttgc agcaccaaag
      721 aagcaaaaaa gataaatata tctacttatt gagagatgat atatttattt ccaaaatgct
      781 acagatacat catcatgaaa cggatcaccg caaacaggtt actcgcctgg ggattccctt
      841 tcgacccgag catccgtatg agactcatgc tcgattatta ttattataga agcccccatg
      901 aataaatcgc tcatcatttt cggcatcgtc aacataacct cggacagttt ctccgatgga
      961 ggccggtatc tggcgccaga cgcagccatt gcgcaggcgc gtaagctgat ggccgagggg
     1021 gcagatgtga tcgacctcgg tccggcatcc agcaatcccg acgccgcgcc tgtttcgtcc
     1081 gacacagaaa tcgcgcgtat cgcgccggtg ctggacgcgc tcaaggcaga tggcattccc
     1141 gtctcgctcg acagttatca acccgcgacg caagcctatg ccttgtcgcg tggtgtggcc
     1201 tatctcaatg atattcgcgg ttttccagac gctgcgttct atccgcaatt ggcgaaatca
     1261 tctgccaaac tcgtcgttat gcattcggtg caagacgggc aggcagatcg gcgcgaggca
     1321 cccgctggcg acatcatgga tcacattgcg gcgttctttg acgcgcgcat cgcggcgctg
     1381 acgggtgccg gtatcaaacg caaccgcctt gtccttgatc ccggcatggg gttttttctg
     1441 ggggctgctc ccgaaacctc gctctcggtg ctggcgcggt tcgatgaatt gcggctgcgc
     1501 ttcgatttgc cggtgcttct gtctgtttcg cgcaaatcct ttctgcgcgc gctcacaggc
     1561 cgtggtccgg gggatgtcgg ggccgcgaca ctcgctgcag agcttgccgc cgccgcaggt
     1621 ggagctgact tcatccgcac acacgagccg cgccccttgc gcgacgggct ggcggtattg
     1681 gcggcgctga aagaaaccgc aagaattcgt taactgcaca ttcgggatat ttctctatat
     1741 tcgcgcttca tcagaaaact gaaggaacct ccattgaatc gaactaatat tttttttggt
     1801 gaatcgcatt ctgactggtt gcctgtcaga ggcggagaat ctggtgattt tgtttttcga
     1861 cgtggtgacg ggcatgcctt cgcgaaaatc gcacctgctt cccgccgcgg tgagctcgct
     1921 ggagagcgtg accgcctcat ttggctcaaa ggtcgaggtg tggcttgccc cgaggtcatc
     1981 aactggcagg aggaacagga gggtgcatgc ttggtgataa cggcaattcc gggagtaccg
     2041 gcggctgatc tgtctggagc ggatttgctc aaagcgtggc cgtcaatggg gcagcaactt
     2101 ggcgctgttc acagcctatc ggttgatcaa tgtccgtttg agcgcaggct gtcgcgaatg
     2161 ttcggacgcg ccgttgatgt ggtgtcccgc aatgccgtca atcccgactt cttaccggac
     2221 gaggacaaga gtacgccgca gctcgatctt ttggctcgtg tcgaacgaga gctaccggtg
     2281 cggctcgacc aagagcgcac cgatatggtt gtttgccatg gtgatccctg catgccgaac
     2341 ttcatggtgg accctaaaac tcttcaatgc acgggtctga tcgaccttgg gcggctcgga
     2401 acagcagatc gctatgccga tttggcactc atgattgcta acgccgaaga gaactgggca
     2461 gcgccagatg aagcagagcg cgccttcgct gtcctattca atgtattggg gatcgaagcc
     2521 cccgaccgcg aacgccttgc cttctatctg cgattggacc ctctgacttg gggttgatgt
     2581 tcatgccgcc tgtttttcct gctcattggc acgtttcgca acctgttctc attgcggaca
     2641 ccttttccag cctcgtttgg aaagtttcat tgccagacgg gactcctgca atcgtcaagg
     2701 gattgaaacc tatagaagac attgctgatg aactgcgcgg ggccgactat ctggtatggc
     2761 gcaatgggag gggagcagtc cggttgctcg gtcgtgagaa caatctgatg ttgctcgaat
     2821 atgccgggga gcgaatgctc tctcacatcg ttgccgagca cggcgactac caggcgaccg
     2881 aaattgcagc ggaactaatg gcgaagctgt atgccgcatc tgaggaaccc ctgccttctg
     2941 cccttctccc gatccgggat cgctttgcag ctttgtttca gcgggcgcgc gatgatcaaa
     3001 acgcaggttg tcaaactgac tacgtccacg cggcgattat agccgatcaa atgatgagca
     3061 atgcctcgga actgcgtggg ctacatggcg atctgcatca tgaaaacatc atgttctcca
     3121 gtcgcggctg gctggtgata gatcccgtcg gtctggtcgg tgaagtgggc tttggcgccg
     3181 ccaatatgtt ctacgatccg gctgacagag acgacctttg tctcgatcct agacgcattg
     3241 cacagatggc ggacgcattc tctcgtgcgc tggacgtcga tccgcgtcgc ctgctcgacc
     3301 aggcgtacgc ttatgggtgc ctttccgcag cttggaacgc ggatggagaa gaggagcaac
     3361 gcgatctagc tatcgcggcc gcgatcaagc aggtgcgaca gacgtcatac tagatatcaa
     3421 gcgacttctc ctatcccctg ggaacacatc aatctcaccg gagaatatcg ctggccaaag
     3481 ccttagcgta ggattccgcc ccttcccgca aacgacccct ggaagccccg tagcgacgcg
     3541 gagaggggcg agacaagcca agggcgcagg ctcgatgcgc agcacgacat agccggttct
     3601 cgcaaggacg agaatttccc tgcggtgccc ctcaagtgtc aatgaaagtt tccaacgcga
     3661 gccattcgcg agagccttga gtccacgcta gatctatctc atctgcgcaa ggcagaacgt
     3721 gaagacggcc gccctggacc tcgcccgcga gcgccaggcg cacgaggccg gcgcgcggac
     3781 ccgcgccacg gcccacgagc ggacgccgca gcaggagcgc cagaaggccg ccagagaggc
     3841 cgagcgcggc cgtgaggctt ggacgctagg gcagggcatg aaaaagcccg tagcgggctg
     3901 ctacgggcgt ctgacgcggt ggaaaggggg aggggatgtt gtctacatgg ctctgctgta
     3961 gtgagtgggt tgcgctccgg cagcggtcct gatcaatcgt caccctttct cggtccttca
     4021 acgttcctga caacgagcct ccttttcgcc aatccatcga caatcaccgc gagtccctgc
     4081 tcgaacgctg cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc
     4141 gagagcggag cctgttcaac ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc
     4201 tcctcaagca cggccccaac agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc
     4261 ccggccgaaa aacccgcctc gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc
     4321 ggtgcgcccg gtcgcgtgcc ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc
     4381 tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc
     4441 accgaatgcg tatgattctc cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc
     4501 ttgttcctga agtgccagta aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg
     4561 cgtgtcgtca gaccgtctac gccgacctcg ttcaacaggt ccagggcggc acggatcact
     4621 gtattcggct gcaactttgt catgcttgac actttatcac tgataaacat aatatgtcca
     4681 ccaacttatc agtgataaag aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga
     4741 ggccagacgt gaaacccaac agacccctga tcgtaattct gagcactgtc gcgctcgacg
     4801 ctgtcggcat cggcctgatt atgccggtgc tgccgggcct cctgcgcgat ctggttcact
     4861 cgaacgacgt caccgcccac tatggcattc tgctggcgct gtatgcgttg atgcaatttg
     4921 cctgcgcacc tgtgctgggc gcgctgtcgg atcgtttcgg gcggcggccg gtcttgctcg
     4981 tctcgctggc cggcgctgct gtcgactacg ccatcatggc gacggcgcct ttcctttggg
     5041 ttctctatat cgggcggatc gtggccggca tcaccggggc gactggggcg gtagccggcg
     5101 cttatattgc cgatatcact gatggcgatg agcgcgcgcg gcacttcggc ttcatgagcg
     5161 cctgtttcgg gttcgggatg gtcgcgggac ctgtgctcgg tgggctgatg ggcggtttct
     5221 ccccccacgc tccgttcttc gccgcggcag ccttgaacgg cctcaatttc ctgacgggct
     5281 gtttcctttt gccggagtcg cacaaaggcg aacgccggcc gttacgccgg gaggctctca
     5341 acccgctcgc ttcgttccgg tgggcccggg gcatgaccgt cgtcgccgcc ctgatggcgg
     5401 tcttcttcat catgcaactt gtcggacagg tgccggccgc gctttgggtc attttcggcg
     5461 aggatcgctt tcactgggac gcgaccacga tcggcatttc gcttgccgca tttggcattc
     5521 tgcattcact cgcccaggca atgatcaccg gccctgtagc cgcccggctc ggcgaaaggc
     5581 gggcactcat gctcggaatg attgccgacg gcacaggcta catcctgctt gccttcgcga
     5641 cacggggatg gatggcgttc ccgatcatgg tcctgcttgc ttcgggtggc atcggaatgc
     5701 cggcgctgca agcaatgttg tccaggcagg tggatgagga acgtcagggg cagctgcaag
     5761 gctcactggc ggcgctcacc agcctgacct cgatcgtcgg acccctcctc ttcacggcga
     5821 tctatgcggc ttctataaca acgtggaacg ggtgggcatg gattgcaggc gctgccctct
     5881 acttgctctg cctgccggcg ctgcgtcgcg ggctttggag aaattcttca aattcccgtt
     5941 gcacatagcc cggcaattcc tttccctgct ctgccataag cgcagcgaat gccgggtaat
     6001 actcgtcaac gatctgatag agaagggttt gctcgggtcg gtggctctgg taacgaccag
     6061 tatcccgatc ccggctggcc gtcctggccg ccacatgagg catgttccgc gtccttgcaa
     6121 tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc agccgaaatg
     6181 cctgccgttg ctagacattg ccagccagtg cccgtcactc ccactcttac attgttcgca
     6241 gtatctaggt acatgttatt gcgtgcacct tcatgatgta taatgttatt aatgacagca
     6301 gaaagatcga cagaattaat attcttcgta cctgggacag ttatcgggtg tcggccagtg
     6361 atgagaccaa aggatgcagt ataagatgaa ccactactat tatcatggcc accattacca
     6421 ccaccgttaa aactacctcc cgaacttcca gagccgctat gaccatcatt gtaggggtca
     6481 tttggtccca tgtgaccaaa accaccgaca gtaccgctat ttaactctgt actcataaaa
     6541 ttatcctctt tgacttttaa aaacagcaaa aactgtattt ataacaatgt gtttatatac
     6601 agtattcgaa atactacgcc cgtcaaaacc acaccgtcaa caggtttttt gtgtattttt
     6661 cacgcctcac gggcagcctc agaccttttg tgttaaaaca gtaatatgtt ctgctgttac
     6721 aatacggtac acacaggaga cactgtcact tttatggcat ggcacacagt tcaagagaca
     6781 ctgaaattaa caggaaagtc gcgctcgcaa ttatatcgtg acatggctaa aggccttgtc
     6841 agctatcgca caggaaaaaa tgatcgccgg gagttcgaga ccagtgaact gattcgggcc
     6901 tatggcgagc tgcgcacttc tgagacacca aaaaggcaca aggagggaca ctccaaatct
     6961 gtcgttagcc aggcggaaaa tgctcagcta gacgtcattc agcagaaact ggacgctctg
     7021 cagcagactg ttaccctaat gcttgaagac aagatggcgc gggaagcaga aaaccgcctg
     7081 cgaaatgagg agagggaaca actacaggcc gaaattagcc gtctggctga cgcgctggca
     7141 caggaaaaaa agcggggatt ctggtcgcgg ctgtttcgct ccggttcaga gtaacagtgc
     7201 aggtgactct gatttaccat aacatctgtt atcagcaccg cctgagaggc cctcagggcg
     7261 tttctgacgg gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg
     7321 caggctctgt ttttacggca tgcatgagta tgcgcacaag tgcatagcgc ggaaattcac
     7381 accattcagc cggggtaagt cctgtttccg gcgttcttcc catccgaaaa cggtgaacgg
     7441 ggacgcaatt tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa cccgctgtca
     7501 gcacgttcag gaagggggtg atgtacaaat ggcacagagg atgaaagtga cgtatgagct
     7561 gaaggaatga tgtaaaagtg atgtataagt gatgtaaaag tgatgtataa gtgatgtaaa
     7621 agtgatgtat gaactgaagg agcgatgtaa ttctgtagtc aggaagaata acccgttcat
     7681 gcaggcatga acgatacagt gaggaacgct ttttttgtca cgatctcccc ttaacctgcg
     7741 taaatcaccc gccatggttg attatttttc aacctcaaaa tggtcataca cctaacgcag
     7801 ccgcgatttt catcgcaact gcatgaatta aaagaaaaaa aaggaatatt tcgttttcac
     7861 gagtaatacc cgggtattaa aaatccactg taattacatg aaaataaaga aataaaaggt
     7921 aataccggag tattaccacg tacgcgggca agcgaaaaag ctataacgct tcccggccga
     7981 caggcgaccc aaaatccaca ggggcatgca ggttctcttc agtaacctgc gccattaaat
     8041 caaaaaggtc atattcgccg ggaagctgcg tatcagtcga atccagttgt tcatcatatt
     8101 cagatggcat ggggtgatat ccagtagctg agaggttgtt agcgggtttc ggggcgcagc
     8161 cctgaaccag tcatgtagcg ctagcggagt gtatactggc ttactatgtt gccacgattg
     8221 cgaaaatcag tgaagtgctt catgtggcag gagaaaaaag gcagcaccgg cgcgtcagca
     8281 gaatatgcga tacaggagat attccgcttc ctcgctcact gactcgctac gctcggtcgt
     8341 tcggctgcgg cgagcggtac cggcttacgg acggggacgg aaatttcctg gaagatacca
     8401 g
//
