LOCUS       NZ_CP118935             9131 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Acinetobacter pittii strain ML4 plasmid pML4_2, complete sequence.
ACCESSION   NZ_CP118935
VERSION     NZ_CP118935.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33440631
            Assembly: GCF_029025705.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Law,M., Leung,K.M., Lai,G.K.K. and Griffin,S.D.J.
  TITLE     Complete genome sequence of Acinetobacter pittii ML4, a
            toluene-degrading strain isolated from Hong Kong soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   Law,M., Leung,K.M., Lai,G.K.K. and Griffin,S.D.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2023) Shuyuan Molecular Biology Laboratory, The
            Independent Schools Foundation Academy, 1 Kong Sin Wan Road,
            Pokfulam, Hong Kong HK SAR, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-FEB-2023
            Assembly Method                   :: Unicycler v. 0.4.8-beta
            Assembly Name :: Marcus_Acinetobacter_pittii_ML4_44Flow_5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 249.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029025705.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:55:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,979
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,806
            CDSs (with protein)               :: 3,806
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 30 of 76
            Pseudo Genes (incomplete)         :: 37 of 76
            Pseudo Genes (internal stop)      :: 18 of 76
            Pseudo Genes (multiple problems)  :: 7 of 76
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118935.1.
            Bacteria and source DNA available from Dr. Simon D. J. Griffin,
            Shuyuan Molecular Biology Laboratory, The ISF Academy, 1 Kong Sin
            Wan Road, Pokfulam, Hong Kong.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9131
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="ML4"
                     /isolation_source="soil"
                     /db_xref="taxon:48296"
                     /plasmid="pML4_2"
                     /geo_loc_name="Hong Kong: Pineapple Dam"
                     /lat_lon="22.38455095 N 114.14407797 E"
                     /collection_date="2022-05-24"
                     /collected_by="Marcus Law"
     gene            complement(join(8967..9131,1..126))
                     /locus_tag="PX335_RS19885"
                     /old_locus_tag="PX335_19880"
     CDS             complement(join(8967..9131,1..126))
                     /locus_tag="PX335_RS19885"
                     /old_locus_tag="PX335_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_000176412.1"
                     /translation="MTLTIRYKKQFKKDFKRVSSNPKLKKGLLILKDLIDEHLVKTGTV
                     PDEYLPHPLIGEWKPHMECHILPDLLLIWDVDEDNNELILARIGSHSELFG"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(110..385)
                     /locus_tag="PX335_RS19890"
                     /old_locus_tag="PX335_19885"
     CDS             complement(110..385)
                     /locus_tag="PX335_RS19890"
                     /old_locus_tag="PX335_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140357.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_005140357.1"
                     /translation="MNTLAKKDEYVRARVPNELKVQAERILKEVGLNTTDAIRLLLTQV
                     VNRGGFPLELRTPNRTTIEAMNSVAEPKKFSSAQELFTDLEDDTDD"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            complement(390..533)
                     /locus_tag="PX335_RS19895"
                     /old_locus_tag="PX335_19890"
     CDS             complement(390..533)
                     /locus_tag="PX335_RS19895"
                     /old_locus_tag="PX335_19890"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162899358.1"
                     /translation="MCYNGFSQINELTTELTTELTTELTTELTTELTTELTTELTTELT
                     TG"
                     /name="hypothetical protein"
     gene            complement(637..861)
                     /locus_tag="PX335_RS19900"
                     /old_locus_tag="PX335_19895"
     CDS             complement(637..861)
                     /locus_tag="PX335_RS19900"
                     /old_locus_tag="PX335_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002072934.1"
                     /translation="MPYEEFQRLIGKSGLSIKEFAALLDMNANSITNYKKNGKVPTTIA
                     VIAVVISDMKDDGLDFYPIFEKVRAYSDQ"
                     /name="hypothetical protein"
     gene            1010..1252
                     /locus_tag="PX335_RS19905"
                     /old_locus_tag="PX335_19900"
     CDS             1010..1252
                     /locus_tag="PX335_RS19905"
                     /old_locus_tag="PX335_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001015364.1"
                     /translation="MNHFVQPKLLISSQAKKIGLALINDLELCITARLAFYLDVNPSYD
                     GHEATFIWIEYFLQDHPQAEFSEVREAFLQLIPGC"
                     /name="hypothetical protein"
     gene            complement(1325..2524)
                     /locus_tag="PX335_RS19910"
                     /old_locus_tag="PX335_19905"
     CDS             complement(1325..2524)
                     /locus_tag="PX335_RS19910"
                     /old_locus_tag="PX335_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002072939.1"
                     /translation="MTPKILCIKSLELEVEKIMSIQEFAFQLSKKIQEQHSVKISRSHI
                     YELIALDQDYRTYSSFVAQNLLLDAQYDDSEEYYQHELVDALTLEILENPPETDYLNYS
                     EDDLHWDDYEGNVLLEQIRKIIIRLKVLLKLDASEEFYLSISKTVYRELLWLNLEVINF
                     NAVREELSYINFENGKVDDLELGGDYFEFSKIGAIFEKILSYAQDRENADAYALLGAYY
                     RYLANQIAPYGRHGSNFGSRWDNNKQKYINSDETKKNKEKYEEYIKQAEHFESYIKNSP
                     MNLHEINLDADKETVYNQILYLCNRGDTSAIEYFLYEEIFKNHGEAWLYIYLAQMCGVD
                     FTQDDFRDYNAYTGEDYDDYGPIEIAGREAIQYAIDLSELDVDKDNLARKIAAELFEKI
                     "
                     /name="hypothetical protein"
     gene            complement(2749..3525)
                     /locus_tag="PX335_RS19915"
                     /old_locus_tag="PX335_19910"
     CDS             complement(2749..3525)
                     /locus_tag="PX335_RS19915"
                     /old_locus_tag="PX335_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000191151.1"
                     /translation="MTQEKAKKRGRPAQLLQMAELHAFVEFLLEKDPRSELQNQVIAAL
                     QAEDFNFEMLSEAQQILVKEALKPYREHLKLQLLFDELVITPGRTEYEEKFLELYQDYK
                     KDDLDPAEMNILKTMCTRYLNFKAQKLEYSDLELYLSQIQKKYAGKKRKAENQRKFEVG
                     GGVLSLYKVKDDENLDSKKFLKRIEIDRFLAGKFRQSQLYKKVWALERPNTEKYQLLFE
                     VLHQLSGYSLKGQTLTLLDLVIINAQKKIKSRSEKE"
                     /name="hypothetical protein"
     gene            3832..4992
                     /locus_tag="PX335_RS19920"
                     /old_locus_tag="PX335_19915"
     CDS             3832..4992
                     /locus_tag="PX335_RS19920"
                     /old_locus_tag="PX335_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_016803209.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIEFWQAADSHERANAKAYMEYEIALPNEFTPEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHENYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQYEYFYSA
                     ENHPFSSLEVMYYLNYLIKNAKNKEKLEKDFSEIIKIQEPILHQEGWNLNNMNRKLRDS
                     EHNSTPRFEFEVHSQPNYPLPMQNKPPQPEQPKPKSDDYDYGGPGF"
                     /name="MobA/MobL family protein"
     gene            5551..6432
                     /gene="repM"
                     /locus_tag="PX335_RS19925"
                     /old_locus_tag="PX335_19920"
     CDS             5551..6432
                     /gene="repM"
                     /locus_tag="PX335_RS19925"
                     /old_locus_tag="PX335_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004734502.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005804946.1"
                     /translation="MNKNHVVKSNQVIEASYQLSAVEQRIVLAAISRIPKNQPITDDEL
                     YPVSVDELKQLGVHEKTAYRDLKEGINRLYERSINLSIDDKSIKMRWVQEIQFLDSQSV
                     IGIRFSKPILPFISNLSREFTKYALSDIAGINSGYGIRIYELLVQYRQIGKREISVESL
                     RTMLELGKKYPLFADFKKRVIDTAIDQINEYSPLKVTYEQKKTGRKVTHIIFSFKEKSK
                     SITHTSKDFYQLSDAQINLFGNQLSRLHELSHLASEGESYEILAAKIKDMLRNPHQQQQ
                     FLPHLKNLGFKA"
                     /name="repM"
     gene            6496..7062
                     /locus_tag="PX335_RS19930"
                     /old_locus_tag="PX335_19925"
     CDS             6496..7062
                     /locus_tag="PX335_RS19930"
                     /old_locus_tag="PX335_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000858429.1"
                     /translation="MKTSTKYSVSDAATLYKKSRATLYKDIKNGVLSRDHDGSIDFSEL
                     LRVYGEPFSGKHSKHTDTSPTQTINTPEYTDNTVIELKNQIDFLKKQLEKAEDREAKAN
                     SRIDTLLTLIEMKPTQVHPTPTSDQSSVQSSPSQVEPTLNVDNDKHDGLTTPEKTENKR
                     IPVPEPVEPEQPKRGWLSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            7413..7730
                     /locus_tag="PX335_RS19935"
                     /old_locus_tag="PX335_19930"
     CDS             7413..7730
                     /locus_tag="PX335_RS19935"
                     /old_locus_tag="PX335_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065053.1"
                     /translation="MLGENHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            8255..8776
                     /locus_tag="PX335_RS19940"
                     /old_locus_tag="PX335_19935"
     CDS             8255..8776
                     /locus_tag="PX335_RS19940"
                     /old_locus_tag="PX335_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000999915.1"
                     /translation="MNDSDDRNHKVILEYLQKVLERERDRFYKLDDKVSKLFTTVSTVI
                     TGFLIIVNILLDHNKISIFCSSEITILSLLSLTTFCFLCMSWNFLFKVFNLKKTAHLQH
                     QGFWKYYKNREEIDFYERIIKDHETVIDTYIESNDQKVLLLKKSLSNIYLSAIMFVILV
                     IIYLIFKYLK"
                     /name="hypothetical protein"
     gene            8773..8919
                     /locus_tag="PX335_RS19945"
                     /old_locus_tag="PX335_19940"
     CDS             8773..8919
                     /locus_tag="PX335_RS19945"
                     /old_locus_tag="PX335_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000192351.1"
                     /translation="MTQKNSNTSTNPANQQPHKDIKSEVPPPPVRYGLDEKIKAEVPKS
                     NKK"
                     /name="hypothetical protein"
     Typing          5552..6430
                     /name="rep_cluster_1364"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.922"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3833..4923
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.703"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtttttacc aaatgctcat caataagatc cttcaaaatt aagagtcctt tcttaagctt
       61 tgggtttgag cttacccgtt taaaatcttt tttaaattgt ttcttgtacc taatcgtcag
      121 tgtcatcttc taaatccgta aacaattcct gagcagaact aaatttttta ggttctgcaa
      181 cagagttcat agcctcaatt gttgtacggt taggggttct taactctaaa ggaaatccac
      241 ctcgatttac tacttgcgtt aataataagc gaatagcatc tgtagtattt aaacccactt
      301 cttttagaat tcgctccgct tgaaccttta attcatttgg aaccctagca cgtacatact
      361 catctttttt tgccaaagta ttcatatgtt tatccagtag tcagttcagt agtcagttca
      421 gtagtcagtt cagtagtcag ttcagtagtc agttcagtag tcagttcagt agtcagttca
      481 gtagtcagtt cagtagtcag ttcattgatt tggctaaagc cattgtagca cacatgtgct
      541 acacaacaac taacttaaca taaaatctca tctacgaaat gcagcagcac gtgctaggat
      601 ccaattaaat ctttaattgg gttcacatca aatactctat tgatcactat aagctcgaac
      661 tttctcaaaa attggataaa agtccagacc atcatccttc atatctgaaa ttacgactgc
      721 aattactgca atcgtagtgg gcactttacc gttcttttta tagttggtaa ttgaatttgc
      781 gttcatatct aaaagcgctg caaattcttt aatagaaagg ccagacttac ctataagtct
      841 ttgaaattct tcgtaaggca tagttttaaa agcagaattg ttctgatgga aaagtatagc
      901 acagatcatt tatttacatt taaacacata aataatgtgt ttaacacatt atttatgtgt
      961 ttaaatggtc ttatcaagca attttactct tttgatagga cttgttttta tgaaccattt
     1021 cgtacagccc aagcttctta tttcttctca agcaaaaaag attggattgg cattaattaa
     1081 cgatcttgaa ctttgtataa cagcacgatt ggctttttat ctggatgtaa atccttccta
     1141 tgacggccat gaggcaacat tcatttggat cgaatatttt ttacaagatc atcctcaagc
     1201 cgaattttct gaagttagag aagcctttct acaacttatc ccaggatgtt aaacgatgat
     1261 aatgaaatct gtattttatg agtcaaatac agatttcaaa ttctcttttt tataagacta
     1321 tgaattaaat tttctcaaat aactctgcag caatcttcct agctaaatta tctttatcaa
     1381 catccagttc tgataaatct atagcatatt gaattgcctc acgacctgcg atttcaatag
     1441 gtccatagtc atcataatcc tcacctgtat aagcattgta gtctctaaaa tcatcctgcg
     1501 tgaaatcaac tccacacatt tgagctaagt agatgtataa ccatgcttct ccatgatttt
     1561 tgaaaatttc ttcatacagg aagtattcaa tagcactcgt atcacctcga ttacacaagt
     1621 acagaatctg gttgtaaact gtttccttgt ctgcatccaa attaatttca tgaagattca
     1681 taggtgaatt tttaatataa gattcaaagt gttccgcttg ctttatgtat tcttcatact
     1741 tttctttatt tttcttagtc tcatcagaat tgatatattt ttgtttatta ttgtcccaac
     1801 gacttccaaa atttgagcca tgtcgaccat aaggggctat ctgatttgct aaataacgat
     1861 agtaggcccc gagtagagca taagcatcag cattctcacg atcttgcgca taagataaaa
     1921 tcttctcaaa aatagcccca atttttgaaa actcgaaata atcacctcca agttctaggt
     1981 catccacctt accattttca aaattgatat aacttaattc ctctcttaca gcattgaagt
     2041 taataacttc taaattaagc cataaaagtt ctcgatagac tgttttagaa atcgacaaat
     2101 aaaactcttc tgaggcatct aacttaagta aaaccttaag tcttataata atttttctaa
     2161 tctgctccaa tagaacatta ccttcataat catcccaatg aagatcatct tcactataat
     2221 ttagataatc tgtttcaggc ggattttcca atatttctag agttagagca tcaaccaatt
     2281 cgtgctgata atactcttca gaatcatcat attgagcatc aagtaaaaga ttttgagcaa
     2341 caaaagagct ataggtccta tagtcctgat ctaaagcaat taattcataa atatgtgaac
     2401 ggctaatctt tacagaatgc tgttcctgaa tttttttaga taattgaaat gcgaattctt
     2461 gaatagacat aattttctct acttccaact ctaaactctt tatacaaaga atctttggtg
     2521 tcataagact tctagcaacg catgtttaaa tgctaagaac caaacgagtt gatacttaag
     2581 aaaattagat tttagatatg gctttaaaaa ttaaacctat ggttttttat cccttttcta
     2641 ctcatgcgta agtacggtag gcgaccttat ttttaaaata ataaatttcg cataaattta
     2701 caattgccaa aatagaaaag tcagctaaaa agcggacagt caaaaaattc attctttttc
     2761 agacctactt tttatctttt tttgagcatt tataatcact aaatcgagaa gcgttaaagt
     2821 ctgccctttt aggctatatc cagacagctg atggagaacc tcaaaaagta gctgatactt
     2881 ttctgtattt ggtcgctcta gtgcccaaac ctttttataa agttgagatt gacgaaattt
     2941 cccggcaaga aaacggtcaa tttcgattct ttttagaaac tttttactgt ctaaattttc
     3001 atcatctttt actttataaa gacttaaaac cccaccaccc acttcaaact tacgttgatt
     3061 ttcagccttt cgctttttcc cggcatactt tttctggatc tgactcaaat acagctccaa
     3121 gtcactgtat tcaagctttt gcgctttaaa attcagataa cgcgtacaca tcgttttcag
     3181 gatattcatt tcagccggat ctagatcatc ttttttataa tcttggtaca gctccaggaa
     3241 tttttcttca tactcagtcc ttcctggagt tatcaccaac tcatcaaaca acaactgaag
     3301 tttcaaatgc tcacggtacg gttttaacgc ttctttcacc agaatctgct gagcttctga
     3361 caacatttca aaattaaagt cttcagcttg tagtgcagca ataacctgat tttgcagttc
     3421 agagcgagga tctttttcta acaggaactc aacgaaagcg tgtaactctg ccatttgtaa
     3481 aagttgagct ggtctaccac gcttttttgc tttttcttgg gtcatatcat ctgccgccta
     3541 attttcagaa ctgctaccga tttaaatatg gttttaccat atttaaatcg ggcagtctga
     3601 aaataattat ttaaattaaa aataaaattt cttgaaaaga aattttattt ttaatttaaa
     3661 taattcgcac ttgacagttt cttctaatcg aagaaactag tacgcaatgg ggcgattccc
     3721 ccttgacccc ctttaaccgg atttctcagc cattcaggca tttcaaaatc cggacggttt
     3781 tcattccgtt tcactgcata aaaaccggtc cccaagccac cagaggtgaa catggccagc
     3841 taccatttca gtgtaaaaag caaaaataaa ggctatgcga ccggccacta cctctatatt
     3901 tcccggctga tgcaatatga aggcattcga aaaacaagca atgaaaccat agaacatatt
     3961 gaacctggac gttgcatgcc cgcctttgtc aaagatccga ttgagttttg gcaggcagcc
     4021 gatagtcatg aacgagccaa tgccaaggcc tacatggagt atgaaattgc cctccccaac
     4081 gagttcacgc cagaacagcg taaaacgctc atagagacgt ttttagacaa acacatagtc
     4141 ccacagcaat atcctcactc atacgccata cacaacgtaa aatcacgcat cagtggcgaa
     4201 gatcagcctc attgtcacct gatgttctcg ctaaaagcgg atgacggcat tgaacgcagc
     4261 gcagaacagt acttcaaacg ctataaccct caagatccag ccaaaggcgg tgctaaaaaa
     4321 atccaacttc aagatggcca tgaaaactat tccacttttt tgatctatat ccgaaaacaa
     4381 tgggaaaatc atttgaatga tgcccttgca cagcactgcc cgaccgtcac ctacacactg
     4441 gatggccaag acatcaccat caaaaatcag gtttcagcag acagctatga aaaatataac
     4501 gagatccaca gcacgctata ccttccagag ccaaaactag gcgttggcca acaaaatgcg
     4561 acagcggaat atctcgctca aatccaaaaa atccgagaac acaaccagca agaacgagaa
     4621 ctcgaagaac gccaaaagca attagatcaa caatatgaat atttttatag tgctgaaaac
     4681 catccatttt catcattgga agtgatgtat tacctgaatt acttaatcaa aaatgcgaaa
     4741 aataaggaaa aactggaaaa agattttagt gaaattatca aaattcaaga gcctatactg
     4801 caccaagaag gctggaacct gaataacatg aatcgaaaat tgagagattc agaacataac
     4861 tcaacgccac gctttgagtt tgaagtccat tcccagccaa attatccatt acccatgcaa
     4921 aataagcccc ctcaacccga acagccaaag ccaaaatctg atgattatga ttacggtggt
     4981 cctggttttt aaaattatct ggctttattc aggccttaca gagcgagtgt ctacgagcga
     5041 acagacccaa ttaaaatttt cgccatgctt tttccctgat tacaaactcc cctttgctgc
     5101 tgagactgag atacagagca tcccggaggg tgcgaacaga tttaatttgc atctaggtcg
     5161 agcgaagcga gtataaaaaa cttatgagcg aagcgaattc cgagcgaatt ccgagttgct
     5221 tttgcttttt tgaagttatc acgagattaa cccaaaaatt gccccttcga actgagcgta
     5281 agcgaagttc aatagagttt gagcgtagcg aaaacatagg gcaatttttc attctttcct
     5341 ttttaatata ttttaaatct tttaaatctt ttcagtcgtc tctgtgcctt atatagcaaa
     5401 ggtttcaacg tttaaatatg acggattgac tactaactat gacggattga ctactaacta
     5461 tgacggattg actacttaac tatgacggat tgactactaa ctatgacaag tatatatttg
     5521 tcatatatta gaaaaatcaa tggttttgtt ttgaataaaa atcacgttgt aaaatcaaat
     5581 caagtaatag aagcatcgta tcaattaagt gccgtagagc aacgaattgt cttagctgcc
     5641 atttcacgga ttcccaaaaa tcagcctatt acagatgatg agttataccc tgttagcgtc
     5701 gatgaattaa aacaattggg cgtacatgag aaaactgcat acagagattt aaaggaaggt
     5761 attaatagac tctatgagag gtctattaac ctcagtattg atgataaatc tataaaaatg
     5821 agatgggtgc aagaaatcca atttttagat agtcaaagtg tcattggaat ccgtttttca
     5881 aaacccattc tgccattcat ttccaatcta agtagagaat tcaccaaata tgccttatca
     5941 gacattgctg ggatcaatag tggatacggt attcgtattt atgagctact ggttcaatat
     6001 cggcaaatag gtaagcgtga aatttctgtt gagagtttac ggacaatgtt agaactcggt
     6061 aaaaagtacc cattattcgc tgatttcaag aaacgagtaa ttgacactgc tatagaccaa
     6121 atcaatgaat atagcccttt aaaagtcact tatgagcaga aaaaaacagg gcgtaaagtc
     6181 actcatatta tcttttcttt taaagaaaaa tctaaatcca taacacatac ctcgaaagat
     6241 ttttatcagc tatctgatgc tcaaattaat ctattcggga atcaattatc acgtttacat
     6301 gagctttccc atttagcctc agaaggtgaa agttatgaga ttttagctgc taaaattaag
     6361 gatatgctta gaaaccctca ccaacaacag cagtttttgc cacacttaaa gaacttaggg
     6421 tttaaagctt gagtgtattg aagaatacac ttattgtatt cttcaatacg tatcctaaat
     6481 acacagcaat acactatgaa aacaagtaca aaatacagtg ttagtgatgc agccacactg
     6541 tataagaaat ctcgtgctac actttataaa gatataaaaa atggggtgtt aagtcgtgat
     6601 catgatggct ctatcgactt ctctgagtta ctccgcgttt atggtgaacc ttttagtgga
     6661 aaacactcaa aacacactga tacgtctcca acacaaacta taaatacacc tgaatacaca
     6721 gacaatacag ttattgaact aaagaatcag atcgattttc taaagaaaca gttagaaaaa
     6781 gcagaagatc gcgaagctaa agcaaattct cgtattgata cattacttac cctaattgaa
     6841 atgaagccaa ctcaggttca tcccacacca acttcagatc aatcttcagt gcaatctagt
     6901 ccatctcaag tagaacctac attgaatgta gataacgaca aacatgacgg attgactact
     6961 ccggaaaaaa cggaaaataa gcgtattccg gtgccggagc cggttgaacc ggagcagccg
     7021 aaaagagggt ggttaagccg gtttttcttg ccgaatggct aagggtacta ttcaactgag
     7081 ctaatcaaag ttgtaaggat ttgattaaag aatagccata ttacgtgaaa gatcacaaaa
     7141 aaaagcccgt acagggatgt tcgggctata aactttgaaa taagtatatg aatttcaagg
     7201 taaattatga cacatttcgt ataaggtgta ttatgttaat aatataaatt tttaaaagca
     7261 tattgattta ataatagaaa aattatcaga gtagggcatg tgtaaagtaa acgttcgatt
     7321 attaaccaat aatgattaaa gatttatatc ttgtatgtgt taattttatt aacatatata
     7381 tacgaaatta ataatgcctt aagtgaaata gtatgctagg tgaaaatcat cattttttaa
     7441 aaaaattttc gatcagtgat gctgctatag cgtataaaaa acctcgctcg actatactta
     7501 aatatataaa aagcggagat ttcgaacttg actacaataa attgattgaa tacagtcagt
     7561 tagttagaaa atttggtgaa cttacttacc ctatacagga cgaaagagag caacaaatca
     7621 aaaaactaaa agatgaagtt atgcatttaa aaaaacaact taactttttt aaatatactt
     7681 caatctcaaa caaaaataga ctattaattt ctcataaaca ttccatttaa cataatgggc
     7741 gttatccgaa atcgtctttt ataagttaaa taaaatcatg tacttagcta ttttacataa
     7801 cccgaaacta ccgcaaaaat ccgctttgtt tccaaagcgg attttttttg agcaaggttg
     7861 atacttcttg ccaataaaat tggttaaata atgatcaaaa atagcaattc catagagttt
     7921 ttaagccttt tttacccaaa agaatccaca atttatgcgg ataaccattg ggataaattt
     7981 tgattatttt ttgtacgttt tggcttacag cgttttacga aattgtctgt atctatcccc
     8041 cgatgaagtc cttatgatca atctatatga acaagatgtt caatgactta aaacaacaat
     8101 aataacaata ataatcagtc aggcaccatg accatcaggt agtcagccct acctaggcgc
     8161 tccacaccga aaaatcccga catgatgtcg ggatttttta ttgttaaaat ttaaattaaa
     8221 taaattttgt tatttgaaat aaaggggatt gagtatgaat gactctgatg atcgtaatca
     8281 taaggtaatt cttgagtatc tacagaaagt actagaaaga gagcgagatc gattttataa
     8341 attagatgac aaagtatcta agttatttac gacagtatct actgtgatta caggatttct
     8401 aattatagtt aatatccttt tagatcataa taaaattagt attttttgtt cttccgagat
     8461 tactattcta agtttattgt cacttacaac attctgcttc ttgtgcatgt cttggaactt
     8521 cctttttaaa gtttttaatt taaaaaaaac agctcacctt caacatcaag ggttttggaa
     8581 atattataaa aatagagaag aaatagattt ttatgaaaga atcattaagg atcatgaaac
     8641 tgttatagat acatacatag aatctaatga ccaaaaagtt ttacttttaa agaagtcttt
     8701 aagcaacatc tatttatcag caataatgtt tgttatatta gttattatct atttgatttt
     8761 taagtatcta aaatgaccca aaaaaattcc aatacaagta ccaacccagc taatcaacaa
     8821 cctcacaaag atatcaaatc agaagttcca cctcctcctg ttagatatgg tttagatgaa
     8881 aaaattaaag ctgaagttcc aaaaagtaat aaaaaataat ctctaattat tgacaacaaa
     8941 aagcccgaat atatcgggct ttttacttat ccaaaaagtt cagaatgaga tccaatccga
     9001 gcaagtatta gctcattatt atcttcatcc acatcccaaa ttaataataa atcaggtaaa
     9061 atatggcatt ccatatgggg tttccactca ccaatcaaag gatgaggtaa atactcatct
     9121 ggaacagtcc c
//
