LOCUS       NZ_CP118905             3681 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Klebsiella pneumoniae strain CRKP plasmid pCRKP-4, complete
            sequence.
ACCESSION   NZ_CP118905
VERSION     NZ_CP118905.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33420447
            Assembly: GCF_029025965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Tang,B.
  TITLE     Outer membrane vesicles transmitting blaNDM-1 mediate the emergence
            of carbapenem-resistant hypervirulent Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Tang,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2023) Department of Clinical Laboratory, Xiangya
            Hospital, Central South University, Xiangya road, changsha, hunan
            410000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 198.0x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029025965.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 05:10:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,826
            CDSs (total)                      :: 5,700
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 67 of 190
            Pseudo Genes (incomplete)         :: 124 of 190
            Pseudo Genes (internal stop)      :: 31 of 190
            Pseudo Genes (multiple problems)  :: 27 of 190
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118905.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCRKP-4"
                     /geo_loc_name="China: changsha"
                     /collection_date="2018-02-23"
     gene            1..258
                     /locus_tag="PXG74_RS29050"
                     /old_locus_tag="PXG74_29050"
     CDS             1..258
                     /locus_tag="PXG74_RS29050"
                     /old_locus_tag="PXG74_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            366..521
                     /locus_tag="PXG74_RS29055"
                     /old_locus_tag="PXG74_29055"
     CDS             366..521
                     /locus_tag="PXG74_RS29055"
                     /old_locus_tag="PXG74_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(679..840)
                     /locus_tag="PXG74_RS29150"
     CDS             complement(679..840)
                     /locus_tag="PXG74_RS29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049165871.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            1245..1436
                     /locus_tag="PXG74_RS29060"
                     /old_locus_tag="PXG74_29060"
     CDS             1245..1436
                     /locus_tag="PXG74_RS29060"
                     /old_locus_tag="PXG74_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1436..1858
                     /locus_tag="PXG74_RS29065"
                     /old_locus_tag="PXG74_29065"
     CDS             1436..1858
                     /locus_tag="PXG74_RS29065"
                     /old_locus_tag="PXG74_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2606..3151)
                     /locus_tag="PXG74_RS29070"
                     /old_locus_tag="PXG74_29070"
     CDS             complement(2606..3151)
                     /locus_tag="PXG74_RS29070"
                     /old_locus_tag="PXG74_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048289822.1"
                     /translation="MPLPERMKPARVTRKQMKELISQLNGILDHIDNGMDENNEELKQM
                     MADWNAKVVMPCGFSDFRDFSSWTSALDFTRMALNQEKYLDDFTWKELNDVINFIRKAE
                     GSASDHSYALQLLEINFKANPLDLIYHPDCWFNDEALFHARLTTEEIGGYLMKKSGRWL
                     NDAPDIQLVYAIPQDVYD"
                     /name="hypothetical protein"
     gene            <3189..3680
                     /locus_tag="PXG74_RS29075"
                     /old_locus_tag="PXG74_29075"
                     /pseudo=""
     CDS             <3189..3680
                     /locus_tag="PXG74_RS29075"
                     /old_locus_tag="PXG74_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /name="S-type pyocin domain-containing protein"
     Typing          complement(728..1197)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.834"
                     /coverage="92.0"
                     /evalue="2.73e-170"
ORIGIN
        1 atggctggta ataaaatttc agattttacg gaagaagaat ttactgcttt tgttagtaaa
       61 ataatcgctg ctgattttcc aactgacaaa gagaacgatg atgctattta ttcgttcgcc
      121 caactaacag agcatcctgc tggttggaac ctcatcttca agcctaaggt aggcgaagat
      181 tcttcggcga aaggaattgt taaaaccgta aaggaatggc gagctgctaa cggtaagcca
      241 ggttttaaaa aagcgtgatt gaatttgcgg gttcaagtta tgagcccgct catttgtaac
      301 tctggatttg aagaataatt actagatatc catttgccta gaataataaa aataaaaggt
      361 gatatatgaa aaaattactt ttcctagggg ttgttaccgt ttctgtggtt ctggcagcat
      421 gtcaggtcaa taatgttcga gacaccggag gtggggctgt ttcaccttca tcgacggtga
      481 ccggagtcac gcttgatagc ggtacgaacg gcaatccgta aaggatcttc ttgagatccc
      541 atttggatcg tcgtaatctc ttgctctgta aacgaaaaaa ccgccttgca gggcggtttt
      601 tccgaaggtt caaacacttg caggtgattg aaccaaggta actggcttgg aggagcgcag
      661 tcaccaaaac ttgttctttc agtttagcct taacaggcgc atgacttcaa gaccaactcc
      721 tctaaatccg ttaccagtgg ctgctgccag tggcgctttg tcgtgtcttt ccgggttgga
      781 ctcaagatga tagttaccgg ataagacgca gcagtcggac tgaacggggg gttcgtgcat
      841 acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca
      901 aacgcggcca taacagcgga atgacaccgg taaaccgaat ggcaggaaca ggagagcgca
      961 cgagggagcc atcaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1021 actgatttga gcgtcaaatt ctgtgatgct tgtcaggggg gcggagccta tggaaaaacg
     1081 gccgctgtgc tgccttctcc ctctccgagc tgtctgctct atgtcgttat gtgcgcgttg
     1141 cgaccgctcg ccgcagccga acggcaggag cgtagcgact gagtgagcga ggaagcggaa
     1201 aagagatctg tctgacattg agcactgacg ctctgaggcc ctttatgacg aaacaacaga
     1261 aaacagcgct gaacatggct aaattcatcc agaatcagtc tctgctcctg ctggagaagc
     1321 ttaacgagct tgatctcgat gttgaggccg acctttgtga gaaactccac gatgatgcgg
     1381 agcatctttt ccgtacactg tcctcgagac tggatgaatt acaggatggt aattgatggc
     1441 cagagtgtct attagtgaag cggcacggct tgttaaggtt agccgtccca caatttacaa
     1501 gatgattaac agtgggaagt taagttacac ctctgttgta aagcacggga aggctattaa
     1561 agttattgat acttcagagc ttattagagt gtttggttct cttgatggtg tcatcgatac
     1621 tgtaaaatat gatgtaaaaa gtgacgcaga atctacaagt attaacagcg ttggtttaca
     1681 tgatttacaa catagaatcg ctctacttga agctgaaaac gatggtctta aaggggctgt
     1741 taaggctcga gatgagcata ttgattccct tcgtcaggct atgcagcttc tggagcacaa
     1801 gcgcgagcct tcttcaccac cccattcccc gtggtggaaa ttctggaaaa aatcctgatc
     1861 ctgccatgct tgagttttcc tctccggcca tcccagggag ttcctgaacc ttcccaaaaa
     1921 tcgccagatc tctgaacgct cctcacgact aaaaataccc acacactacg caaaattaaa
     1981 attttgcaga ttccttattt aaaacaattg gttaatgaaa aattaatgtg cagtgatgtg
     2041 ttacaaagtg cagatccaag tcgtggacga ctctatggcg aatcgtttac gaattgccca
     2101 cgattcatat gtgaatcacg ctttattatc atgttgttat gatgaatcga ctcttgattc
     2161 aattcatcaa tttgggcgtt ttttgttcat ggtgactctt gattcaattc acgattcgaa
     2221 tcgccggtga atcgtgtggt gagtcgtccg gcgacttgcc ataacgtgat tgccggccgt
     2281 cgacgaaccc gattcctgaa cgtgctgaaa accggttttc cggctgcgcc ggcaacgcct
     2341 cacggccacg caataaattg cgtccccgtt caccgttttc ggatcagaag aacgccggaa
     2401 acaggactta ccctggctga atggcgtgaa tttccgcgct atgcacttgc gcgcatactc
     2461 atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc
     2521 tgttttaccg gtttcccgtc agaaacgtcc tgaggccgtt ttagcggtgc gtacaattaa
     2581 gggattatgg taaatggtga cttggttaat cataaacatc ctgggggatg gcatacacca
     2641 gttggatatc cggtgcatcg ttgagccatc gacctgattt tttcattaag tagccaccaa
     2701 tttcttcggt agtcagccgg gcatgaaaca atgcttcatc attgaaccag cagtcagggt
     2761 gatagatgag atccagtggg tttgccttga aattaatctc cagtaactgg agtgcatagc
     2821 tgtggtcaga tgcactacct tctgctttgc gtatgaagtt gataacgtcg ttcagttctt
     2881 tccatgtaaa gtcatcgagg tatttttcct gattaagcgc catcctggtg aaatctagtg
     2941 cacttgtcca ggatgaaaaa tcacgaaaat cagagaaccc gcagggcatg acgacctttg
     3001 cattccagtc tgccatcatc tgttttagtt cttcattgtt ttcgtccatc ccgttatcga
     3061 tgtgatcgag aatgccattc agctgactga tcagttcttt catctgttta cgggtgaccc
     3121 gtgcgggttt cattcgctca ggtaatggca tcagaaatac tctctttttt atcgtgcact
     3181 cgggggaact gagtaatcta gcggatgacc tcgattttga cgatattatt ctggtgtttc
     3241 ctccggaatc cggcctaaaa ccgctgtatg tgatgtaccg tagccctcgt aacatgcccg
     3301 gtacagtgag cggcaaaggg cagaacgttg gcaataactg gatggggggg gccagtaccg
     3361 gggatggtgc tcctgttcct tcccagattg cggataaatt acgcgggaag actttcggta
     3421 gttttgattc tttccgtcgg gcgttctgga aagcggttgc tgacgattct gcactgagca
     3481 agcaatttag tgaagctgat attaatcaga tgaaagctgg aagggcacct actgcggact
     3541 ttttagaatc agtagggaaa cgtgttaaaa ttgaattaca ccacgaaaaa gaaatatctc
     3601 aaggaggggc tgttatggat gtcgataaca ttaaggcgct aacacctaaa aatcatattg
     3661 agacacataa aggaaaataa a
//
