LOCUS       NZ_CP118855             4431 bp    DNA     linear   CON 15-MAY-2024
DEFINITION  Mammaliicoccus lentus strain 7047 plasmid pML7047-5.
ACCESSION   NZ_CP118855
VERSION     NZ_CP118855.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33407026
            Assembly: GCF_030012925.1
KEYWORDS    RefSeq.
SOURCE      Mammaliicoccus lentus
  ORGANISM  Mammaliicoccus lentus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Mammaliicoccus.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Belhout,C., Stegger,M., Vereecke,N., Elgroud,R. and Butaye,P.
  TITLE     Diversity and characterization of Methicillin Resistant
            Staphylococci (MRS) and Mammaliicocci (MRM) isolated from camels in
            Algeria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Belhout,C., Stegger,M., Vereecke,N., Elgroud,R. and Butaye,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 206x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030012925.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 04:23:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,635
            CDSs (total)                      :: 2,554
            Genes (coding)                    :: 2,509
            CDSs (with protein)               :: 2,509
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 14 of 45
            Pseudo Genes (incomplete)         :: 18 of 45
            Pseudo Genes (internal stop)      :: 15 of 45
            Pseudo Genes (multiple problems)  :: 2 of 45
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118855.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Mammaliicoccus lentus"
                     /mol_type="genomic DNA"
                     /strain="7047"
                     /isolation_source="Nose"
                     /host="Camelus dromedarius"
                     /db_xref="taxon:42858"
                     /plasmid="pML7047-5"
                     /geo_loc_name="Algeria: M'sila"
                     /collection_date="2021-12-13"
     gene            <1..1380
                     /gene="tet(K)"
                     /locus_tag="PYH49_RS13150"
                     /old_locus_tag="PYH49_13145"
     ARG             2..1377
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1567..2809
                     /gene="mobV"
                     /locus_tag="PYH49_RS13155"
                     /old_locus_tag="PYH49_13150"
                     /pseudo=""
     CDS             1567..2809
                     /gene="mobV"
                     /locus_tag="PYH49_RS13155"
                     /old_locus_tag="PYH49_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            3338..4327
                     /locus_tag="PYH49_RS13160"
                     /old_locus_tag="PYH49_13155"
     CDS             3338..4327
                     /locus_tag="PYH49_RS13160"
                     /old_locus_tag="PYH49_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009385482.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_282840369.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYNRELKKEIS
                     PVDLTDLMKSTLKANEKQLQKILKINLKIKIKKKIIKKKKKNKKNINY"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          3339..4243
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.448"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          1568..2442
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.973"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtttagtt tatataaaaa atttaaaggt ttgttttata gcgttttatt ttggctttgt
       61 attctttcat tttttagtgt attaaatgaa atggttttaa atgtttcttt acctgatatt
      121 gcaaatcatt ttaatactac tcctggaatt acaaactggg taaacactgc atatatgtta
      181 actttttcga taggaacagc agtatatgga aaattatctg attatataaa tataaaaaaa
      241 ttgttaatta ttggtattag tttgagctgt cttggttcat tgattgcttt tattggtcac
      301 aatcactttt ttattttgat ttttggtagg ttagtacaag gagtaggatc tgctgcattc
      361 ccttcactga ttatggtggt tgtagctaga aatattacaa gaaaaaaaca aggcaaagcc
      421 tttggtttta taggatcaat tgtagcttta ggtgaagggt taggtccttc aataggggga
      481 ataatagcac attatattca ttggtcttac ctacttatac ttcctatgat tacaatagta
      541 actatacctt ttcttattaa agtaatggta cctggtaaat caacaaaaaa tacattagat
      601 atcgtaggta ttgttttaat gtctataagt attatatgtt ttatgttatt tacgacaaat
      661 tataattgga cttttttaat actcttcaca atcttttttg tgatttttat taaacatatt
      721 tcaagagttt ctaacccttt tattaatcct aaactaggga aaaacattcc gtttatgctt
      781 ggtttgtttt ctggtgggct aatattttct atagtagctg gttttatatc aatggtgcct
      841 tatatgatga aaactattta tcatgtaaat gtagcgacaa taggtaatag tgttattttt
      901 cctggaacca tgagtgttat tgtttttggt tattttggtg gttttttagt ggatagaaaa
      961 ggatcattat ttgtttttat tttaggatca ttgtctatct ctataagttt tttaactatt
     1021 gcattttttg ttgagtttag tatgtggttg actactttta tgtttatatt tgttatgggc
     1081 ggattatctt ttactaaaac agttatatca aaaatagtat caagtagtct ttctgaagaa
     1141 gaagttgctt ctggaatgag tttgctaaat ttcacaagtt ttttatcaga gggaacaggt
     1201 atagcaattg taggaggttt attgtcacta caattgatta atcgtaaact agttctggaa
     1261 tttataaatt attcttctgg agtgtatagt aatattcttg tagccatggc tatccttatt
     1321 attttatgtt gtcttttgac gattattgta tttaaacgtt ctgaaaagca gtttgaatag
     1381 ttatattata ttttggttta gaactatgag tggctagcat tttgccactc attttttgcg
     1441 ttagcaaaaa caggtttaag cctcgcagag cacacgtatt aacgacttat tgtaaaataa
     1501 gtctagtgtg ttagacttaa actattaaat acacatgaaa cctttgtgct taggagtgat
     1561 ttttatatgt cttattccat tgttagagtt tcaaaagtta aatctggaac aaatacaacg
     1621 ggcatacaaa aacatgttca aagagaaaat aataattatg aaaatgaaga tatagaccat
     1681 agtaaaactt acttaaatta tgatttggta aatgctaata aacagaattt taataacttg
     1741 attgatgaaa aaatcgaaca gaattataca ggcaaaagaa aaattagaac agacgcgatt
     1801 aaacacattg atggtttaat tacatcagac aatgatttct ttgataatca aacgccagaa
     1861 gatacaaagc agttttttga atatgctaaa gagtttttag aacaagaata cggtaaagat
     1921 aatttattat atgcaacagt tcacatggac gaaaaaacac cacatatgca ttatggcgtt
     1981 gttccaataa ctgatgatgg tcgtttaagt gctaaagaag ttgtaggtaa taaaaaagct
     2041 ttaacagcgt ttcaagatag atttaatgag catgttaaac aacgaggata tgatttagaa
     2101 cgtgggcaat caagacaagt aacaaatgct aaacatgagc aaataagtca gtataaacaa
     2161 aaaacagaat atcataagca agaatatgaa cgtgagagcc aaaaaacaga ccatataaag
     2221 caaaagaacg ataaattaat gcaagagtac caaaaatcgt taaatacgct taaaaagcct
     2281 ataaatgttc cgtatgagca agaaactgaa aaagtaggtg gtttatttag caaagaaata
     2341 caagaaactg gaaatgttgt aataagccaa aaagatttca atgaatttca gaaacagata
     2401 aaagctgctc aagatatttc ggaagattac gatatatata aagtctggta gagccttaga
     2461 tgataaagat aaggaaatac gagagaaaga tgatttatta aataaagcag ttgagcgtat
     2521 tgaaaacgca gacgataatt ttaaccaact ttacgaaaat gcaaagccac ttaaagagaa
     2581 tatagaaata gcgttaaagc ttttaaaaat cttactaaaa gagttagaac gagttttagg
     2641 aagaaatacc tttgcggaaa gagttaataa gttaacagaa gatgaaccaa aactaaatgg
     2701 tttagcagga aacttagata aaaaaatgaa tccagaatta tattcagaac aggaacagca
     2761 acaagaacaa caaaagaatc aaaaacgaga tagaggtatg cacttataga acatgcattt
     2821 atgccgagaa aacttattgg ttggaatggg ctatgtgtta gctaacttgt tagcgagttg
     2881 gttggacttg aattgggatt aatcccaaga aagtaccaac tcaacaacac ataaagccct
     2941 gtaggttccg accaataagg aaattggaat aaagcaataa aaggagttga agaaatgaaa
     3001 ttcagagaag cctttgagaa ttttataaca agtaagtatg tacttggtgt tttagtagtt
     3061 ttaactgttt accagataat acaaatgctt ataaaaaaaa aagacttgat ctgattagac
     3121 caagtctttt gatagtgtta tattaataac aaaataaaaa ggagtcgctc acgccctgac
     3181 caaagtttgt gaacgacatc attcaaagaa aaaaacactg agttgttttt ataatcttgt
     3241 atatttagat attaaacgat atttaaatat acatcaagat atatattttg ggtgagcgat
     3301 tccttaaacg aaattgagat taaggagtcg attttttatg tataaaaaca atcatgcaaa
     3361 tcattcaaat catttggaaa atcacgattt agacaatttt tctaaaaccg gctactctaa
     3421 tagccggttg gacgcacata ctgtgtgcat atctgatcca aaattaagtt ttgatgcaat
     3481 gacgatcgtt ggaaatctca accgagacaa cgctcaagcc ctttctaaat ttatgagtgt
     3541 agagccccaa ataagacttt gggatattct tcaaacaaag tttaaagcta aagcacttca
     3601 agaaaaagtt tatattgaat atgacaaagt gaaagcagat agttgggata gacgtaatat
     3661 gcgtattgaa tttaatccaa acaaacttac acgagatgaa atgatttggt taaaacaaaa
     3721 tataataagc tacatggaag atgacggttt tacaagatta gatttagcct ttgattttga
     3781 agatgatttg agtgactact atgcaatgtc tgataaagca gttaagaaaa ctatttttta
     3841 tggtcgtaat ggtaagccag aaacaaaata ttttggcgtg agagatagta atagatttat
     3901 tagaatttat aataaaaagc aagaacgtaa agataatgca gatgctgaag ttatgtctga
     3961 acatttatgg cgtgtagaaa tcgaacttaa aagagatatg gtggattact ggaatgattg
     4021 ctttagtgat ttacatatct tgcaaccaga ttggaaaact atccaacgca ctgcggatag
     4081 agcaatagtt tttatgttat tgagtgatga agaagaatgg ggaaagcttc acagaaattc
     4141 tagaacaaaa tataatagag aattgaaaaa agaaatttcg ccagtcgatt taacggactt
     4201 aatgaaatcg actttaaaag cgaacgaaaa acaattgcaa aaaatattaa aaataaattt
     4261 aaaaataaaa ataaaaaaaa aaataattaa aaaaaaaaaa aaaaataaaa aaaatataaa
     4321 ttattaagat ttttttttta ttaagtattt aataaaaaaa gggaaatata taaaaggaga
     4381 aaaaaaaata tacccctata aactttagtc accccaagta aaaaggtaaa a
//
