LOCUS       NZ_CP118778             8331 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Mammaliicoccus lentus strain 7074 plasmid pML7074-2, complete
            sequence.
ACCESSION   NZ_CP118778
VERSION     NZ_CP118778.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33407050
            Assembly: GCF_030013925.1
KEYWORDS    RefSeq.
SOURCE      Mammaliicoccus lentus
  ORGANISM  Mammaliicoccus lentus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Mammaliicoccus.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Belhout,C., Boyen,F., Vereecke,N., Theuns,S., Taibi,N., Stegger,M.,
            de la Fe-Rodriguez,P.Y., Bouayad,L., Elgroud,R. and Butaye,P.
  TITLE     Prevalence and Molecular Characterization of Methicillin-Resistant
            Staphylococci (MRS) and Mammaliicocci (MRM) in Dromedary Camels from
            Algeria: First Detection of SCCmec-mecC Hybrid in
            Methicillin-Resistant Mammaliicoccus lentus
  JOURNAL   Antibiotics (Basel) 12 (4), 674 (2023)
   PUBMED   37107036
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Belhout,C., Stegger,M., Vereecke,N., Elgroud,R. and Butaye,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 324x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030013925.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 04:35:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,551
            Genes (coding)                    :: 2,509
            CDSs (with protein)               :: 2,509
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 11 of 42
            Pseudo Genes (incomplete)         :: 18 of 42
            Pseudo Genes (internal stop)      :: 15 of 42
            Pseudo Genes (multiple problems)  :: 2 of 42
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118778.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Mammaliicoccus lentus"
                     /mol_type="genomic DNA"
                     /strain="7074"
                     /isolation_source="Nose"
                     /host="Camelus dromedarius"
                     /db_xref="taxon:42858"
                     /plasmid="pML7074-2"
                     /geo_loc_name="Algeria: Ouargla"
                     /collection_date="2022-06-06"
     gene            1..168
                     /locus_tag="PYH61_RS13075"
                     /old_locus_tag="PYH61_13075"
     CDS             1..168
                     /locus_tag="PYH61_RS13075"
                     /old_locus_tag="PYH61_13075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_158258750.1"
                     /translation="MNLDKNSKKLIPFKNMSNFDGLFEFCPFIEFEVNSNWEEYLNYLE
                     YVKSINQKIG"
                     /name="hypothetical protein"
     gene            190..618
                     /locus_tag="PYH61_RS13080"
                     /old_locus_tag="PYH61_13080"
     CDS             190..618
                     /locus_tag="PYH61_RS13080"
                     /old_locus_tag="PYH61_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="WP_107566102.1"
                     /translation="MAYDLKPFGKDYFDMTASELFRDFGRQFFSNLPDQKTIKTDINEK
                     EDRYELTAELPGFSKDQIDISYENGMLIISGENNQVNEEKDNEGKVIQKERSYSNVKRM
                     YSLNNIDEDNIEAKFEDGILSVDLPKTENNQRKVIDIK"
                     /name="Hsp20/alpha crystallin family protein"
     gene            1007..1135
                     /locus_tag="PYH61_RS13085"
                     /old_locus_tag="PYH61_13085"
     CDS             1007..1135
                     /locus_tag="PYH61_RS13085"
                     /old_locus_tag="PYH61_13085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259342030.1"
                     /translation="MISIIDVMVGLTNEEKKELENMEMSKVEFKYKLVLTEKMMKC"
                     /name="hypothetical protein"
     gene            1371..2408
                     /locus_tag="PYH61_RS13090"
                     /old_locus_tag="PYH61_13090"
     CDS             1371..2408
                     /locus_tag="PYH61_RS13090"
                     /old_locus_tag="PYH61_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019236158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="WP_107591119.1"
                     /translation="MKAAVWYGQKDVRVEDRKSKELQDNEVKVKVSWAGICGTDLHEYL
                     EGPIFISTDKPDPFLGQKAPVTLGHEFAGVVDDIGSKVTKFNKGDRVVINPTVSNREKE
                     ENIDLYDGYSFIGLGSDGGFAEFTNAPEENVYKLPDNVSDKEGALVEPTAVAVQAIKEG
                     EVLFGDTVAIFGAGPIGLLTVIAAKAAGASKIFVFDLSEERLNKAKAIGATHTINSGES
                     NPSDVINKHTDNGVDVAFEVAGVAPTLKSAIDVTKARGTVVIVSIFGHPIEWNPMQLTN
                     TGVKLTSTIAYTPTTFQQTIDLINEGNLKVKDVITDEIELDNIVESGFEQLVNDKSQAK
                     ILVKL"
                     /name="2,3-butanediol dehydrogenase"
     gene            complement(2496..3047)
                     /locus_tag="PYH61_RS13095"
                     /old_locus_tag="PYH61_13095"
     CDS             complement(2496..3047)
                     /locus_tag="PYH61_RS13095"
                     /old_locus_tag="PYH61_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508689.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_025904408.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVIYKLDRLGRTTKQLIELSQWLDDNSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKHTGE
                     EIAKQTGVSRSTVYRIIKMK"
                     /name="recombinase family protein"
     gene            complement(3453..4190)
                     /gene="erm(B)"
                     /locus_tag="PYH61_RS13100"
                     /old_locus_tag="PYH61_13100"
     ARG             complement(3457..4190)
                     /name="erm(B)"
                     /gene="erm(B)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4315..4398)
                     /locus_tag="PYH61_RS13105"
                     /old_locus_tag="PYH61_13105"
     CDS             complement(4315..4398)
                     /locus_tag="PYH61_RS13105"
                     /old_locus_tag="PYH61_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]; GO:0031556 - transcriptional attenuation by ribosome
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="WP_001814874.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
                     /name="23S rRNA methyltransferase attenuation leader
                     peptide"
     gene            complement(4447..>4530)
                     /locus_tag="PYH61_RS13110"
                     /pseudo=""
     CDS             complement(4447..>4530)
                     /locus_tag="PYH61_RS13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /name="peptide-binding protein"
     gene            complement(5050..5775)
                     /locus_tag="PYH61_RS13115"
                     /old_locus_tag="PYH61_13110"
     CDS             complement(5050..5775)
                     /locus_tag="PYH61_RS13115"
                     /old_locus_tag="PYH61_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_119480185.1"
                     /translation="MKSVKMLSEELKVSKQTIFNNIKRLNIETIKQENTSFIKDETDIE
                     RIIQRVNENKKKYGFESNTADIKNNESDNVESENDTQIIEILKHQIDTLNRQIEKQESR
                     HETTLEFYRKELQERSKLLENQQILALESNKKIQKLENQLEEERHLNYPFDTSNNDRKN
                     VDAQETTDTTDSVNINQDQESEMQYEDISERQSHESTQDDVAPKEDDLSNSNDNDSDIE
                     EKSEETQHKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            6195..7175
                     /locus_tag="PYH61_RS13120"
                     /old_locus_tag="PYH61_13115"
     CDS             6195..7175
                     /locus_tag="PYH61_RS13120"
                     /old_locus_tag="PYH61_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_282822282.1"
                     /translation="MSRKNVNNQASQNFYILHKALFINEKYKKLSDSSKIGYAVLNDRV
                     SLSIKNNWIDQNGDIYFIFTNENLQEVLGKSKNTITKIKKELEEVGLLEQVKTGFNRPN
                     KLYLHEIETNINVEKQIQKNTENNDELSNSKDSQTLGVQNPKVWESRIPNFGSPESQSL
                     DSNDTELSNTKLNNTEYNDMNDLNDIEHKHNYHSDHSYQFVTESDDKDYKEILLQEFPE
                     ELTNYLMNYHYKDLEVIKTVILKAKKSFNSRHEDMHYMLEDIEDEILISLKRVKKAIHD
                     RGVKGQKETIISMQGYLMRTILSELEELYSADMRRQNMTKYNIFN"
                     /name="replication initiator protein A"
     gene            <7192..7485
                     /locus_tag="PYH61_RS13125"
                     /old_locus_tag="PYH61_13120"
                     /pseudo=""
     CDS             <7192..7485
                     /locus_tag="PYH61_RS13125"
                     /old_locus_tag="PYH61_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568503.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     ARG             complement(3454..4190)
                     /name="ErmB"
                     /gene="ErmB"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="97.96"
                     /coverage="98.79"
     Typing          complement(5051..5775)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.819"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaatttag ataaaaattc aaaaaaacta attcctttta agaatatgtc taattttgat
       61 ggtttatttg agttttgtcc ttttatcgaa tttgaagtta atagtaattg ggaagaatat
      121 ttaaattatt tggagtatgt caaaagtatt aatcaaaaaa taggttaatt attataagga
      181 gtgattatca tggcttatga tttaaaacca tttggaaaag attattttga tatgactgct
      241 agtgaattgt ttagagactt tggaagacaa ttttttagta atttacctga ccaaaaaact
      301 ataaaaacgg atattaatga aaaagaagat agatatgaat taacagctga gcttccaggc
      361 ttttcaaaag atcaaataga tatttcttat gaaaatggta tgttaatcat ttctggagaa
      421 aataatcagg tgaatgaaga aaaagataat gaaggtaaag taattcaaaa agaaagatca
      481 tatagtaatg tgaagagaat gtattcactt aataatatag acgaagataa tattgaagct
      541 aaatttgaag atggtatttt atctgtagat ttaccaaaaa ctgaaaataa tcaaagaaaa
      601 gtaatagata tcaagtgata gttaaaaaga gatgtgaatc tgatttcaca tctctttttt
      661 catttttatt cctcttttgc tttttatttt ttaaacgagt gaaacgagtt tcttcttgtc
      721 ttgatatgac tgagttcaac ttctttctta tcgttaatga atttgaccat aagaaaagtc
      781 gttaactctc aaattaagga gagttaacga ctaaagcaaa agttactcat actgtataat
      841 gtttatttta tatataaata ttaatcttaa tggatgtatg tatcaagata tatacttgct
      901 tgtgtatatc ttgtaaattt atggttatga taatagtcga gatatactgg aattttggtt
      961 tattttcgtt tttttagaaa cgagtgaaaa cgagtttctt cttttcttga tatcaataat
     1021 agatgttatg gttggcttaa ccaatgaaga gaaaaaagaa ttagaaaata tggaaatgag
     1081 taaagttgaa tttaaatata aactcgttct tacagaaaaa atgatgaaat gctaggacaa
     1141 taagcagtta aaatagaagt tcaacacatg tgcaataaac acttgtgttg agtgtacatg
     1201 tgttagttaa ctaaattatc tatatgaggt atgattaaaa gtaattgtat tattgaggta
     1261 cactctaatc atataataat ttagaaacac tatattaaaa ggttagttta tttgaaagta
     1321 atttttaaaa ggcatattat taaattaact aaaacaagga ggttttatca atgaaagcag
     1381 cagtttggta tggtcaaaag gatgtacgag ttgaagatcg aaaatctaaa gagttacaag
     1441 ataatgaggt taaagttaaa gtgtcttggg ctggcatttg tggtacagat ctacatgaat
     1501 acttagaagg ccctatattt atttcaacag acaaaccaga cccgttcctt ggccaaaaag
     1561 cgcccgttac attaggccac gaatttgcag gcgtagtaga tgatattggt tctaaggtta
     1621 caaaatttaa caaaggcgat cgtgttgtta ttaatcctac agtttcaaat cgtgaaaaag
     1681 aagaaaatat tgacctttat gatggttatt catttatagg cttaggttct gatggtggat
     1741 ttgcagaatt tacaaatgcc ccagaagaga atgtttataa attaccagat aatgtttctg
     1801 ataaagaagg cgcccttgta gaaccgacgg ctgttgcagt tcaagcaatt aaagaaggcg
     1861 aagttctatt tggcgatact gttgctattt ttggtgcagg tccaatcggt ttattaacag
     1921 tcatagcagc taaagctgca ggtgcaagta agatatttgt ttttgattta tcagaagaaa
     1981 gattgaataa agctaaagca ataggtgcta ctcatactat caattctggt gaatcaaacc
     2041 caagtgatgt tattaataaa catacagata atggcgtgga tgtagcattt gaagtagctg
     2101 gtgtagcacc aacacttaaa tctgctatag acgttactaa agcaagaggt actgtcgtta
     2161 tagtgtctat atttggtcat cctattgaat ggaatccaat gcaattaact aatacaggtg
     2221 taaaacttac ttcaacgatt gcttatacac ctactacatt tcaacaaaca atagatttaa
     2281 ttaatgaagg taatttaaaa gttaaagatg ttattactga tgaaatagag ctagataata
     2341 ttgtggagtc aggatttgaa caacttgtaa acgataaatc acaagcaaaa attctcgtta
     2401 aattataata tagattataa aaactaatga aaggagaatc tctaatttga agattctcct
     2461 ttcattagtt ttctttctgt gattatttgt ttttactact tcatttttat tattctataa
     2521 acagtagacc tgctaacacc tgtttgcttc gcaatttctt cacccgtatg tttttgttca
     2581 tcatataaga acttgatttc tcttttcttg tggtcaggta atgaagggcg accacctttt
     2641 cgacctcttg ctcttgctgc ttctagtcct tttttagttc gttcacttaa taagttagct
     2701 tcaagttccg caaatgcact catcatagta aagaacatct tgcccatcgc atctttggtt
     2761 gatacgttca tatctataat atgtaaatca atactattat catctaacca ttgagataac
     2821 tcaattaatt gcttggttgt tcggccaaga cgatctagtt tatagataac taaaatatcg
     2881 ccttcacgta aataatctaa gcatttatca agttccgttc gtttcgtttt gcgaccactc
     2941 gctttctcgc taaagatacg ttcacaacca tattcctcaa gtgtatcaat ctgtccatca
     3001 agactttgat cttgtgttga tacacgtgca taaccaattt tagccatttg aataccacct
     3061 cttttaatcg ttttatgagt acttactaat gaaatctagt tctcgatgca aataaacgcg
     3121 gctctcatcg tctgaaaact catatttttt ggtatttatg atacattgat tataacacaa
     3181 gtttttagac ttcgatatcc aagaaaaccc tcttaatatt aagttaagag ggtttgtatc
     3241 aaaaacgatc gtttatggta ccattaaaat ttatccttat tgtacaaaat aacagcgaaa
     3301 tttttaaatc tattccttat cgatacaaat tccccgtagg cgctagggac ctctttagct
     3361 ccttggaagc tgtcagtagt atacctaata atttatctac attcccttta gtaacgtgta
     3421 actttccaaa tttacaaaag cgactcatag aattatttcc tcccgttaaa taatagataa
     3481 ctattaaaaa tagacaatac ttgctcataa gtaacggtac ttaaattgtt tactttggcg
     3541 tgtttcattg cttgatgaaa ctgattttta gtaaacagtt gacgatattc tcgattgacc
     3601 cattttgaaa caaagtacgt atatagcttc caatatttat ctggaacatc tgtggtatgg
     3661 cgggtaagtt ttattaagac actgtttact tttggtttag gatgaaagca ttccgctggc
     3721 agcttaagca attgctgaat cgagacttga gtgtgcaaga gcaaccctag tgttcggtga
     3781 atatccaagg tacgcttgta gaatccttct tcaacaatca gatagatgtc agacgcatgg
     3841 ctttcaaaaa ccactttttt aataatttgt gtgcttaaat ggtaagggat attcccaaca
     3901 attttatacc tctgtttgtt agggaattga aactgtagaa tatcttggtg aattaaagtg
     3961 acacgaatgt tcagttttaa tttttctgac gataagttga atagatgact gtctaattca
     4021 atagacgtta cctgtttact tattttagcc agtttcgtcg ttaaatgccc tttacctgtt
     4081 ccaatttcgt aaacggtatc ggtttctttt aaattcaatt gttttattat ttggttgagt
     4141 actttttcac tcgttaaaaa gttttgagaa tattttatat ttttgttcat gtaatcactc
     4201 ctgaagtgat tacatctata aataaataca gaagttaaac gatttgtttg taattttagt
     4261 tatctgttta aaaagtcata agattagtca ctggtaggaa ttaatctaac gtatttattt
     4321 atctgcgtaa tcactgtttt tagtctgttt caaaacagta gatgttttat ctacattacg
     4381 catttggaat accaacatga cgaatccctc cttcttaatt acaaattttt agcatctaat
     4441 ttaacttcaa ttcctattat acacaatttt aagataatgc actatcaaca cactcttaag
     4501 tttgcttcta agtcttattt ccataacttt agggttaacc atacgcaaga ccaatcactc
     4561 tcggacaata ctcatgattt taatgataaa atccatgtta aacccataga taagaaatac
     4621 acctgcaata accgttacct gtttgtgcca atttaaaaat gcaccacttt tttataatta
     4681 aaacggttga aaactgtacc actaataact cacaatagag agatgtcacc gtcaagttaa
     4741 atgtacaaaa taacagcgaa atttttaaat ctatttctta tcgatacaaa ttcctcgtag
     4801 gcgctcggga cccctaattg tgtttcgttg cgcaataata cacgctcata ccccaaatct
     4861 tgtagctgtt taatcacatt ttcttctaaa ccatattcac tttggtatcc caaattaatc
     4921 gctccaccct attaaattta agtataatta aatattaaca tatcaaaaat taaatatatg
     4981 atataaaaaa cgctgcaagt tctttaactt gtagcgtttt tttaataaat atagatttta
     5041 aaacaataat tagctaccaa ataaacggct ccagaatcct tttttatgtt gcgtttcttc
     5101 acttttttct tcaatatcac tatcattgtc atttgaatta gatagatcat cttcctttgg
     5161 tgctacatca tcttgtgtac tttcatgact ttgtctctca gatatatcct catattgcat
     5221 ctctgattct tggtcttgat taatgtttac agaatcagta gtatctgtag tttcttgtgc
     5281 atcaacgttt tttctatcgt tattagatgt atcaaaggga taattaaggt gcctctcttc
     5341 ctctaattgg ttttctagtt tttgaatttt tttattgctt tctaaagcta atatttgttg
     5401 attttcaagc aactttgatc tttcctgtaa ctcttttcta tagaattcta atgttgtttc
     5461 gtgacgactt tcttgctttt caatctgcct atttaaggtg tcaatttgat gtttaagtat
     5521 ttctattatc tgagtatcat tttcagactc aacattatcc gattcattat tttttatatc
     5581 cgctgtatta ctttcgaaac catatttctt tttattttca tttacccttt gaatgattct
     5641 ttctatatca gtttcatctt taataaatga agtgttttcc tgttttattg tctcaatatt
     5701 tagtcgttta atattattaa aaattgtttg cttactaacc tttaattcct cacttaacat
     5761 tttcacactt ttcatacaat caaaccttta taaatagttt gtcaaacgtt tatactctat
     5821 tatataaccg taaccactga aactcaaata tatattaagg aaaataagaa tttttatcaa
     5881 tgctttatct aggattaata aggtttatca atagtttatc aaatctttgt caacctttga
     5941 caaatgtgta ttttctcctt ttacaaccct atttaaagtg atataattaa catatcaaaa
     6001 aaccccaact actggtaata gtcggggggt ggtaataaaa cgttatagca cgtttgttta
     6061 atatcaaatt tgtacttgta ttatataatg aacaacatct tagtgcaagc acctaaatct
     6121 ttaaataaat ttaatgctat ataaaagcgt tttattacct cttaccaaac aaataatgga
     6181 aagaggtatt tattatgtct agaaaaaatg taaataatca agcaagtcaa aatttttata
     6241 ttcttcataa agcattattt attaatgaaa aatacaaaaa gttaagtgat agttcaaaga
     6301 ttggctatgc tgttcttaat gatagagtta gtttatcaat taagaataac tggatagatc
     6361 aaaatggaga tatatatttt atttttacaa atgaaaacct tcaagaagtt ttaggcaaga
     6421 gtaaaaatac catcactaaa ataaaaaaag aattagaaga agtaggattg ttggaacaag
     6481 ttaaaactgg ttttaatcga ccaaacaaac tttacctgca cgaaattgag acaaatatca
     6541 acgttgaaaa acaaatacaa aaaaatactg aaaataatga cgaacttagt aatagcaagg
     6601 actcccaaac tttgggagtc cagaatccca aagtttggga gtccagaatc ccaaactttg
     6661 ggagtccaga atcccaaagt ttagactcta atgatactga acttagtaat actaaattaa
     6721 ataatactga atataatgat atgaatgatt tgaatgatat agaacataag cataattatc
     6781 attcagatca ttcatatcaa tttgtaactg aatctgatga taaagattat aaagaaatat
     6841 tactacaaga atttcctgaa gaattaacta attatttaat gaattatcat tataaagatt
     6901 tagaagttat aaaaactgtt attcttaaag caaaaaaatc atttaattca agacatgaag
     6961 atatgcatta catgttagag gacattgaag atgaaatatt aatatcttta aaaagagtaa
     7021 aaaaagcgat acatgataga ggtgttaaag gccaaaagga aacgatcatt tcgatgcaag
     7081 ggtacttaat gaggacgatt ttaagtgaac ttgaagaact ttattcagcc gatatgagaa
     7141 gacaaaatat gacgaaatat aatattttta attaataaat taaataagct tgcagcaatc
     7201 aaagaaacat caaaatatag tgttaaatca tcagactttc taacaagtaa tcaagaaaaa
     7261 aaccaagaaa tagtcaaaga cttagaacaa ggtttataca gaaaacgtat gctaagctac
     7321 ggtggtttac tcaaacaaaa acacaaaatt ttaaatttag atgactcaga agacgggaac
     7381 ttaattaata catcagacga aaagacaaca gaagaagaac aaaaagcaca ctcaatcatg
     7441 gcaatatgga attatcaaaa acaaaactac tacatcaaaa aataataaaa aaaactgacc
     7501 tcaatcagaa attatgtatt gtgattgagg tcagtttttt tattatgaaa tattattgat
     7561 ttaatagtgt tttgcttttg ataattcttt acttatcgtc aagtcgatgt catgtcgatg
     7621 ttaagtaaaa aaagcttttt tagcctctgc aggaactatg cgattaaacg gcacgtttac
     7681 ccacttttta tgccaatttt taaatttgca tgtaactggg cagtgtctta aaaaatcgac
     7741 actggttttt agaaaaaatc attttgtgaa atcaaaatat atttatttgg ctcatatttg
     7801 cgttttaaga gcgtatataa gtttttggat ataaaactat atgatttacc cctatttctt
     7861 taaaatgacc ccttaaaatt caaaataaag catgtatgag aatgtataaa gtcaaaaaat
     7921 aaagttgatt atagaatcta taagctgttt tttagaatct atcagaatct ataattttat
     7981 aaacgttgat ataagtgact tgtaaagaat ctgaatacaa gttctgacga tgccaggaaa
     8041 cttttataca ggttatacat tttaaaagtg gatcatgtgg cttagttgta tgatttgttt
     8101 ggttttaaca tggcttaaaa gaaatgtata aagcataaga agacacctca gctatgctga
     8161 gtgacacgga gtggcattgt gtccccttat gctcttagga gagaatctga aaagaatctg
     8221 aatacttaaa aatataaagt aaaaaatgaa tagaaaaatt attgaccatt tttgaccttt
     8281 atgttatata tatagtgaaa atagttatat cctttgtagg gaggggttga t
//
