LOCUS       NZ_CP118703             9512 bp    DNA     circular CON 04-APR-2024
DEFINITION  Aeromonas hydrophila strain K522 plasmid pK522-B-KPC, complete
            sequence.
ACCESSION   NZ_CP118703
VERSION     NZ_CP118703.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33408175
            Assembly: GCF_028994095.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas hydrophila
  ORGANISM  Aeromonas hydrophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Luo,X.
  TITLE     The sequence of Aeromonas hydrophila K522
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   Luo,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) Department of Clinical Laboratory, Taizhou
            Municipal Hospital, No.381-1, Zhongshan East Road, Jiaojiang,
            Taizhou 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JAN-2023
            Assembly Method                   :: Unicycler v. v0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028994095.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 06:58:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,081
            CDSs (total)                      :: 4,923
            Genes (coding)                    :: 4,828
            CDSs (with protein)               :: 4,828
            Genes (RNA)                       :: 158
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 119
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 15 of 96
            Pseudo Genes (incomplete)         :: 74 of 96
            Pseudo Genes (internal stop)      :: 13 of 96
            Pseudo Genes (multiple problems)  :: 7 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118703.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Aeromonas hydrophila"
                     /mol_type="genomic DNA"
                     /strain="K522"
                     /host="Homo sapiens"
                     /db_xref="taxon:644"
                     /plasmid="pK522-B-KPC"
                     /geo_loc_name="China: Zhejiang"
                     /collection_date="2020"
     gene            1..945
                     /locus_tag="PWO56_RS25350"
                     /old_locus_tag="PWO56_25350"
     CDS             1..945
                     /locus_tag="PWO56_RS25350"
                     /old_locus_tag="PWO56_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_215270799.1"
                     /translation="MGLLIRPQATALGYSHIQFNPPPHVAMLVFDVDKKHQHDPQRDGY
                     HEWQDRGLPAPHWISINPENGNYHLGYLLASPVARSSVARLGPLRYLAAIEHVLAKRLG
                     ADLRYAGLVTKNPVHSDWMTFWHDIEPYTLDYLAEFCPDADLAAFSGRKRKEASGLGRN
                     IEVFDNVREWAYKAVRRFWRPNGYDAWADAVLAACESANAFGLEQGGPLPVSEIKSTAK
                     SIARWVWRNLTPSAFADYVDRTHTSEIQARRGAKGGKVSKGGGRPSNSGKDKSDLLPEV
                     LRLKAQGYTNRDIADDLQISPSTVSVYLKRDHP"
                     /name="replication initiation protein"
     gene            complement(1144..1425)
                     /locus_tag="PWO56_RS25355"
                     /old_locus_tag="PWO56_25355"
     CDS             complement(1144..1425)
                     /locus_tag="PWO56_RS25355"
                     /old_locus_tag="PWO56_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_082020691.1"
                     /translation="MKDAADQGTGDMFGIRRGRGRPKTGTAKTGAERQATYRAKQQEIN
                     VTVTLNRNDLRAVKTILAFAKVKGITEMVDAETLERIERAIYAIPADE"
                     /name="hypothetical protein"
     gene            1637..2038
                     /gene="mobC"
                     /locus_tag="PWO56_RS25360"
                     /old_locus_tag="PWO56_25360"
     CDS             1637..2038
                     /gene="mobC"
                     /locus_tag="PWO56_RS25360"
                     /old_locus_tag="PWO56_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_039168860.1"
                     /translation="MTKPELKTTTASLRLDPATLAKWRQGASDEGLTLSDWLRAKVDAD
                     QQTHISPARSRRERDPRHLADPALMRQLAALGSNLNQIARAVNECRLVGSSVHLVELLT
                     LLRSIEESAGRLLPQLPPPPNREDDGHDD"
                     /name="mobC"
     gene            2031..>2711
                     /locus_tag="PWO56_RS25365"
                     /old_locus_tag="PWO56_25365"
                     /pseudo=""
     CDS             2031..>2711
                     /locus_tag="PWO56_RS25365"
                     /old_locus_tag="PWO56_25365"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2743..3444
                     /locus_tag="PWO56_RS25370"
                     /old_locus_tag="PWO56_25370"
     CDS             2743..3444
                     /locus_tag="PWO56_RS25370"
                     /old_locus_tag="PWO56_25370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223938277.1"
                     /translation="MEKGGELKTRLSERLAQRQAEELAEVATLTEANLTQLSERLTKHV
                     SAELSTTRDAIRQQSEQITTLLGSQQSALEQQQATVDALKCLSLTDLEQIQKGLNDAIT
                     LAQGKAIEQAIDPLRTALEQLKTDGEALSLVTFRGWLKPLLISMAVILGAGGSTWGVAA
                     YLANEITGQLTELQSLNQQISTAQRTLDSLPKGVTFVRDRDGQYIVARQIDTPFKMDEG
                     TYKGLMAVGVK"
                     /name="hypothetical protein"
     gene            3445..3786
                     /locus_tag="PWO56_RS25375"
                     /old_locus_tag="PWO56_25375"
     CDS             3445..3786
                     /locus_tag="PWO56_RS25375"
                     /old_locus_tag="PWO56_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011116720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039168756.1"
                     /translation="MTELERLLTAQFERMEQRHQDETMDLRAQLDAQQRALKSLQAQLQ
                     STLGEYEKHCASLHTAFVALEKSTRSELASMGRSLDQTNRATGAALGSLNSSVSIVKSS
                     LESLNKAQS"
                     /name="hypothetical protein"
     gene            <4099..4293
                     /locus_tag="PWO56_RS25380"
                     /old_locus_tag="PWO56_25380"
                     /pseudo=""
     CDS             <4099..4293
                     /locus_tag="PWO56_RS25380"
                     /old_locus_tag="PWO56_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            4460..5341
                     /locus_tag="PWO56_RS25385"
                     /old_locus_tag="PWO56_25385"
     CDS             4460..5341
                     /locus_tag="PWO56_RS25385"
                     /old_locus_tag="PWO56_25385"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="WP_004199234.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-2"
     gene            complement(5591..>6166)
                     /locus_tag="PWO56_RS25390"
                     /old_locus_tag="PWO56_25390"
                     /pseudo=""
     CDS             complement(5591..>6166)
                     /locus_tag="PWO56_RS25390"
                     /old_locus_tag="PWO56_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            complement(<6333..6497)
                     /locus_tag="PWO56_RS25395"
                     /old_locus_tag="PWO56_25395"
                     /pseudo=""
     CDS             complement(<6333..6497)
                     /locus_tag="PWO56_RS25395"
                     /old_locus_tag="PWO56_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /name="MFS transporter"
     gene            complement(6507..6764)
                     /locus_tag="PWO56_RS25400"
                     /old_locus_tag="PWO56_25400"
     CDS             complement(6507..6764)
                     /locus_tag="PWO56_RS25400"
                     /old_locus_tag="PWO56_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="WP_019396551.1"
                     /translation="MEDMHKHTTHPDLVKRLKRAEGHLRHVIGMIEGGETCLDIARQLA
                     AVESAVTAAKRVLIHDHIDHCLSHDEDSVLTEMKALTKLL"
                     /name="metal-sensing transcriptional repressor"
     gene            6989..8086
                     /locus_tag="PWO56_RS25405"
                     /old_locus_tag="PWO56_25405"
     CDS             6989..8086
                     /locus_tag="PWO56_RS25405"
                     /old_locus_tag="PWO56_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821153.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]; GO:0008324 - monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_008786738.1"
                     /translation="MSQSHAHDHGHDHDHDHDHTPTVTSANERKVLLSFFLIFGFMVVE
                     AVGGVLSGSLALLADAGHMLTDAAALALAYAAFRFGRRAADSKRTFGYLRFEVIAGFLN
                     AVTLFAIVAWIAYEAWERLQAPPVILAGPMMIVAVLGLLVNVLVLWIMTRGETDHVNVK
                     GAILHVMGDLLGSVGAIVAAIVIYLTGWTPIDPILSVLVAALILRSAWKLLAKSIHILL
                     EGAPEDASPEKVEQGLMGAVPGLAAVSHVHVWQLTSGRTMATLHVRPNVDEEARAVVKR
                     VEAVLREQFSIEHATVGIDWNSGTDENVCSLQPTSERQDHSGHDHSGHDHSGHDHSGHD
                     HGNHKHGGGHSHGHDHDHSAPGHRH"
                     /name="cation diffusion facilitator family transporter"
     gene            8130..8507
                     /locus_tag="PWO56_RS25410"
                     /old_locus_tag="PWO56_25410"
     CDS             8130..8507
                     /locus_tag="PWO56_RS25410"
                     /old_locus_tag="PWO56_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821152.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_247141920.1"
                     /translation="MTSRKTTSADTAQCSTTSHDAATDALAMSQSDVAVLAETFRLLGD
                     QSRLKILLQCMHGSVAVGDIAEALDLSQSLVSHHLRLLRGARLVRGERQAKHIFYSIAD
                     QHVSQVLQDMVVHISEDKADD"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     ARG             4461..5338
                     /name="blaKPC-2"
                     /gene="blaKPC-2"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 atggggctgc tcattcgtcc gcaggctact gcgctgggct atagccacat ccagttcaac
       61 ccgccgccgc atgtcgccat gctggtgttc gacgtggaca agaagcacca gcatgacccc
      121 cagcgcgatg gctaccacga gtggcaggat agggggctgc ctgccccgca ctggatctcg
      181 atcaacccag agaacggcaa ttaccacctt ggttacctgc tggcctcgcc cgtggcgcga
      241 tccagtgtcg ccagattggg gccgttgcgt tacctggctg ccatcgagca cgttctagcg
      301 aaacgattgg gggccgatct gaggtatgca gggctggtca caaaaaaccc cgtccacagc
      361 gattggatga cgttctggca cgacattgaa ccctacaccc tcgactatct cgccgagttt
      421 tgcccagatg ctgatctggc ggcctttagt ggccgtaagc gcaaagaagc gagcgggttg
      481 gggcgtaaca tagaggtgtt cgataacgtg cgggaatggg cctataaggc cgtcagacgg
      541 ttttggcggc ccaacggcta tgacgcatgg gccgatgccg tgcttgcggc ctgtgagagc
      601 gccaatgcgt ttggcttgga gcaaggcggt ccgctgccag tgtccgagat taagtctacg
      661 gccaagagca ttgcccgctg ggtctggcgg aacctaactc cgtccgcgtt tgcggattac
      721 gttgatcgga cacatacctc cgagatccaa gcgcggagag gggctaaggg cggtaaggta
      781 tccaagggag ggggaaggcc gagcaatagc ggtaaggaca agtccgatct attgccggag
      841 gttctgcggc ttaaggctca ggggtacacc aaccgcgata tagcggacga tcttcaaatc
      901 tcccccagta cggtgagcgt ttacctaaag cgtgatcatc cgtgatcgaa acaagcctat
      961 atcagataac agcgcccgaa agggcgttct cgagcttttg ccttttagtc ttaaaaatcc
     1021 ttttagagcg gatccttggc gaggtgggga acggccggac cgaaggccgt ccatcgcccc
     1081 ccggctctat cggggggtgt gggcggggcc gttgtccacg gagcgatctt gctctggccc
     1141 cggttactcg tctgccggga tcgcgtagat cgcccgctct atccgctcca gcgtttccgc
     1201 gtccaccatc tccgtgatcc ccttgacctt agcgaaggcc agaatggttt ttactgccct
     1261 cagatcattc ctgtttaacg tcacggtaac gtttatttcc tgttgcttcg cccggtaggt
     1321 agcctgccgt tctgcccccg tcttggctgt ccctgtcttt ggtctaccac gacctcgcct
     1381 gatgccgaac atgtccccag ttccctgatc cgctgcgtcc ttcatgatgc tctttcctgt
     1441 cactgttacg gtaattcatt atctgtcacg gtgacgttta ttgcaagttg ccgaccccct
     1501 cgttttctgc tttactggta ttaagccagc cgaccccgta ttacattacg cccgcttcgc
     1561 ggtctccatg tatccggggg ctggtgaggg gctccgcccc ttcaaacccc acgctgctac
     1621 gccgcttgga gagccaatga caaagccaga gctaaagaca acaacggcca gccttcgcct
     1681 cgatcctgcc accctggcca agtggcgcca gggggccagc gacgaggggc tgaccctctc
     1741 cgactggctg agagccaagg tggatgccga ccagcaaacc catatatccc ccgcccggtc
     1801 gcggcgggaa cgagaccccc gccacctggc agatcctgcg ctaatgcgcc agctcgcggc
     1861 gttgggatcg aacctcaacc agatcgccag ggcggtgaac gaatgccgcc tggtagggag
     1921 ctcggttcac ctggtggagc tgctgacgtt gctacgtagc atcgaggaga gcgccgggag
     1981 actactgccg cagcttccgc ctccgccgaa ccgggaggat gacgggcatg atgattaagt
     2041 tcctggctca tggcaccggt aaggccagca gtgcggcgac ctatttgctg gacagtcacg
     2101 accacaaggg cgaggaacgt gccggggtgg cggtgttgcg gggtgatccg cagcagttcg
     2161 ccgccgtggc ggactcgctc gattttcagc atcgataccg gagtgcggtg atctcctggt
     2221 cggaggaaga ggcccccagt gacgccgaga tcggcgcggt gctggacgac ttcgaggcgc
     2281 tgtcctttgc cggactggat aggcaggacg tgcatctgac cgccgtgcag caccgggagg
     2341 ccgggggcgg tgtccacgtc cacatcctgg tgccccgtgt tcacctgggc acgggtaaat
     2401 ctctgaacat tgccccgccc ggacatagaa aagactttga cgccctgcgg gataaatgga
     2461 actacggcaa gggctgggcc agaccagacg acccgctccg ggcacggctg atacagccct
     2521 cctttgacgc ctacaagggc cagcaggaca ggactgccat caagcggcag ataggggatc
     2581 acctgcttgg ggcggtggct caggggctga tccgcgaccg gggcgaatta ctcgagtacc
     2641 tgggcgaggt gctcgaattc gagatcaccc gccagggcaa ggactacatc acgatcaggc
     2701 cggagggata cccgcagggc atccggctca aaggagagct gtatggagaa gggtggcgag
     2761 ctgaaaacac gcttgagcga gagactagcg cagcggcaag ccgaggagct ggccgaggtg
     2821 gccacgttga ccgaggccaa tctgacacag ctcagcgagc gtttgacaaa gcatgtgagc
     2881 gccgagctga gtacaaccag ggacgctatt cggcagcaga gcgagcagat caccacgttg
     2941 ttaggttcac agcagagcgc cttggaacaa caacaggcga ccgtggacgc gctcaaatgc
     3001 ttgagcttga cggacttgga gcagatccaa aaggggttaa acgacgcgat cactctcgcc
     3061 caggggaagg caatcgagca ggcgatcgat ccgctcagga cggccctaga gcaactcaag
     3121 acggacgggg aggccctgag tctggtgacg ttccggggat ggctcaaacc gctgctgata
     3181 tcgatggcgg tgatcctagg ggccgggggc agtacctggg gggtggcagc gtacctggcg
     3241 aacgagatca ccgggcaact gaccgagctt cagagcctaa accagcagat cagcacagcc
     3301 caacggaccc tagacagctt gcccaaaggg gtgacgttcg tgcgcgaccg agatgggcag
     3361 tacatcgtgg cgaggcagat cgatacaccg ttcaagatgg acgaagggac gtacaagggc
     3421 ctgatggccg tgggggtgaa gtagatgacc gaattagagc ggctgctgac agctcaattc
     3481 gagcggatgg aacaacggca ccaggacgag acgatggact tgcgggcgca gctcgacgcg
     3541 caacagcgcg ctttgaagag cttgcaggcg cagttgcaga gcacattggg cgagtacgag
     3601 aagcattgcg ccagcttaca caccgccttc gtggccttgg agaagagtac caggagcgag
     3661 ctagccagca tgggccggag tttggaccag accaaccggg ctaccggagc agcacttggg
     3721 tcattgaaca gcagtgtgag catagtcaaa agctcgctgg agagcttgaa caaggcgcag
     3781 agttgatcgt cgatagggcc gcggtgctgc gaaaagagca gagccagagt agcgggttga
     3841 gtatgtaata taacgtcaca gtgacaataa ttaagtagat cgggtatgaa atcaggtcgg
     3901 ttgggtactc ctgacgcggt ggcgcatatt ggggttctag ggattttccg tccaaatgcg
     3961 acattcagac tgctaagtga ttgatttatt gtgactggct atgtgaagcg ccaaaaaagt
     4021 aatacccctc tggaggccgc ggacggcgtg gcctccagag cactttgtcg tttttggacg
     4081 gaaaatccct agaaccccat cctagagcgc acgaatgagg gccgacagga agcaaagctg
     4141 aaaggaatca aatttggccg caggcgtacc gtggacagga acgtcgtgct gacgcttcat
     4201 cagaagggca ctggtgcaac ggaaattgct catcagctca gtattgcccg ctccacggtt
     4261 tataaaattc ttgaagacga aagggcctcg tgatacgcct atttttacat ataggttaat
     4321 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcgttccac tgagcgtcag
     4381 acccctaatc ccagctgtag cggcctgatt acatccggcc gctacaccta gctccacctt
     4441 caaacaagga atatcgttga tgtcactgta tcgccgtcta gttctgctgt cttgtctctc
     4501 atggccgctg gctggctttt ctgccaccgc gctgaccaac ctcgtcgcgg aaccattcgc
     4561 taaactcgaa caggactttg gcggctccat cggtgtgtac gcgatggata ccggctcagg
     4621 cgcaactgta agttaccgcg ctgaggagcg cttcccactg tgcagctcat tcaagggctt
     4681 tcttgctgcc gctgtgctgg ctcgcagcca gcagcaggcc ggcttgctgg acacacccat
     4741 ccgttacggc aaaaatgcgc tggttccgtg gtcacccatc tcggaaaaat atctgacaac
     4801 aggcatgacg gtggcggagc tgtccgcggc cgccgtgcaa tacagtgata acgccgccgc
     4861 caatttgttg ctgaaggagt tgggcggccc ggccgggctg acggccttca tgcgctctat
     4921 cggcgatacc acgttccgtc tggaccgctg ggagctggag ctgaactccg ccatcccagg
     4981 cgatgcgcgc gatacctcat cgccgcgcgc cgtgacggaa agcttacaaa aactgacact
     5041 gggctctgca ctggctgcgc cgcagcggca gcagtttgtt gattggctaa agggaaacac
     5101 gaccggcaac caccgcatcc gcgcggcggt gccggcagac tgggcagtcg gagacaaaac
     5161 cggaacctgc ggagtgtatg gcacggcaaa tgactatgcc gtcgtctggc ccactgggcg
     5221 cgcacctatt gtgttggccg tctacacccg ggcgcctaac aaggatgaca agcacagcga
     5281 ggccgtcatc gccgctgcgg ctagactcgc gctcgaggga ttgggcgtca acgggcagta
     5341 aggctctgaa aatcatctat tggcccacca ccgccgccct tgcgggcggc atggattacc
     5401 aaccactgtc acatttaggc taggagtctg cgcggcagag ccgtgtgacc ggttttctgt
     5461 agagcactga cgatggcggc ggcgctctct gcaattggca aggcgtcggc gccaaggata
     5521 ccaatcttgc ggcgcgcggc gtgttatgac gactggggtg catttgagcc gccccattta
     5581 accttcgccc tcacagatac gccattcgcc tcagatttag cgccatgcag acgagcttcc
     5641 actcggcttg caccttgtcc aggcccctca tgctgaactg acgcaatccc atcaccgcct
     5701 tgatccaacc attcggagcc tccacgatcg acttgcgccg gcggtaagct gcatcgcctt
     5761 gctccgtttt caatttcgcc gcaatcgccg ccgtatgcgg atgggtcttg gcattgacct
     5821 tggcatcttc acgtccctcg cggccgaggg caacgatgac atcgccgtgg tgatcggcga
     5881 cctttgccag aacagcctca ctacggaatc ccgcatccgc cagcgtctgg gccggcattt
     5941 ctccggtgtt ggcctgaact gctgccagca tgcccagcag cgcctgactg tccgcggcgc
     6001 agttggtcaa ctccgccgcc acgatgatct ggtgctcggc atcgaccgct gtgtacccgt
     6061 tgtagctctg ctcggagcca ccaccggcgt gtttcatgat ccggctgtcc ggatcggtga
     6121 agctttcctg atcacggtca tccggcacac caaactcgcg tttgtggggt tctagggatt
     6181 ttccgtccaa atgcgacatt cagactgcta agtgattgat ttattgtgac tggctatgtg
     6241 aagcgccaaa aaagtaatac ccctctggag gccgcggacg gcgtggcctc cagagcactt
     6301 tgtcgttttt ggacggaaaa tccctagaac cccatcttga tggccagcgc cgtcccgagc
     6361 accgcgcctg catccgcgcc ggccagctcg taggccagca ggccgagcgc caccgtcatc
     6421 aggccggtgc cgacgagcgc gatcacctgg gcggtgaaca ggtggcggta ggtgcggtta
     6481 ttcaggacgg agagcatcgg acggcctcag agcagtttgg tcagcgcctt catttcggtc
     6541 agaacggagt cctcgtcatg ggacaggcag tggtcgatgt ggtcgtggat caggacgcgc
     6601 ttggccgccg tcaccgcgct ttccaccgcc gcgagctggc gggcgatgtc gaggcaggtt
     6661 tctccccctt cgatcatccc gatgacgtgc cgcagatgcc cctcggcgcg cttgagccgc
     6721 ttcaccaggt cgggatgggt ggtgtgctta tgcatgtctt ccatgctgaa atcatatccc
     6781 cccagggggg atactgccac tggatgttag atcaacggct gcttgccagg cggggggaat
     6841 ggtcgccgag ccaggctaag gcaattgccc ggagcggcat cccggccgtg gtgagacgcg
     6901 aacacaaatg tgtacaacaa ttcacatgag aacatgttca tgtgttatat tttgagccac
     6961 agtcaaagac ccagtttccc gaggccccat gtcccaatcc cacgcacatg accatggtca
     7021 cgatcacgac catgatcatg accacacccc gaccgtgacc agcgccaacg agcgcaaggt
     7081 cctgctgtcc tttttcctga tcttcggctt catggtcgtg gaggccgtcg gaggcgtgct
     7141 gtcgggctcg ctggccttgc tggccgacgc gggccacatg ctgaccgacg ccgcggcgct
     7201 cgcactggcc tacgcagcat tccggttcgg ccgccgcgcg gccgacagca agcgcacctt
     7261 cggctacctg cgcttcgagg tcatcgccgg tttcctgaac gccgtgaccc tgttcgcgat
     7321 cgtcgcttgg atcgcctacg aggcgtggga gcggctgcag gcgccgcccg tgatcctggc
     7381 cggcccgatg atgatcgtcg ccgtgctcgg cctgctggtg aacgtgctcg tgctgtggat
     7441 catgacccgc ggagagaccg atcacgtcaa cgtgaagggg gccatcctgc acgtgatggg
     7501 cgacctgctc ggctcggtcg gcgccatcgt cgctgcgatc gtcatctacc tcacgggctg
     7561 gacgccgatc gatcccatcc tgtcggtcct ggtcgcggcg ctgatcctgc gcagcgcatg
     7621 gaagctgctc gccaagtcga tccacatcct gctggaaggc gcgccggagg atgcttcgcc
     7681 ggagaaggtg gagcaaggcc tgatgggcgc cgtgccgggc ctggcggccg tcagccacgt
     7741 tcacgtgtgg cagctcacct ccgggcgcac gatggccacg ctgcacgttc gccccaacgt
     7801 ggacgaggaa gcacgcgccg tggtcaagcg cgtcgaagcg gtgcttcgag agcaattcag
     7861 catcgaacac gccacggtcg ggatcgactg gaactcgggc accgacgaga acgtctgcag
     7921 cttgcagccc acgtcggagc gtcaggacca cagcggccac gatcacagcg gtcatgacca
     7981 cagcgggcac gaccacagcg gccatgatca tggcaatcac aagcatggcg gcgggcacag
     8041 ccacggccat gaccacgacc attccgctcc tggtcacagg cattgaggcc gcgcgagccg
     8101 gctcgcactc cacttgagga tttgcatcca tgacgtcccg caaaaccacc agcgccgaca
     8161 ccgcgcaatg ctcgaccacc agccacgacg cggcgaccga tgccctcgca atgtcgcaaa
     8221 gtgacgtcgc tgttctggcc gagaccttcc gcctgctggg cgaccaatcg cgattgaaga
     8281 tcctgctgca gtgcatgcat ggctcggtcg ccgtggggga catcgccgaa gcgctcgacc
     8341 tgtcgcagtc tctggtcagc catcacctgc ggctgctgcg cggtgcgcgc ctcgtgcgcg
     8401 gcgaacgcca agccaagcac atcttctaca gcatcgcgga ccagcacgtc agccaggtgc
     8461 tgcaggacat ggtggtccac atttccgagg acaaagccga cgactgacca ggcccaagca
     8521 cccaggcgaa tggcaggttg cgaggcgccg gtacccacgc cgccggttag cctcaaggac
     8581 gtcctcgatg cggcctgggc gcaacaccct gaatcgcggg ccctgcctgg gcgccgggat
     8641 gccgcgcagg cacagcaatg ggcggcggca gcttggacgc ctgagcctcc cgcgctcgca
     8701 atcacccaca agaccgaccg gttcaaccgc acaacggcac tcgtgagctg gatgtcggag
     8761 tggccacagc gacgacgcgg ggttttccca ccaccttgtc catgcagcct gcccgctagg
     8821 gcaacccaaa gggcttgacc ttgccttcgt tgtaacgcct agcctccaca caaatttctg
     8881 cagagcgctt cggaagggcc ggagtcctgc atccaagaaa ggggcacttc ggggttctag
     8941 ggattttccg tccaaatgcg acattcagac tgctaagtga ttgatttatt gtgactggct
     9001 atgtgaagcg ccaaaaaagt aatacccctc tggaggccgc ggacggcgtg gcctccagag
     9061 cactttgtcg tttttggacg gaaaatccct agaaccccat attcgccagt tccccccggt
     9121 tgagtacgaa ctggcgatct atcaaggctc accgtggcta tgggccgatg gaaagccgct
     9181 gatcgacctc ctcgatgcga cctcttgggg tggtgcagcc atatccgttc ctgctccctt
     9241 atcgggtgca agggggcgtg acgtgttgcg gtagcgaaag cggtgcggta acttgttgtc
     9301 cagaaaagac aaaccccgaa gctctgctga tcaacttggc ggaagactca gagcattcgg
     9361 ggccgttcac aaagaacagg aaaagcataa cacatgcacg atctcagaca accctgcacc
     9421 gccaataagc gcggtgatct ctgactcaga cggcccaaaa cctcttcata gacaggcttc
     9481 cgcgccgccc ctactgcacc aacgacaaat cg
//
