LOCUS       NZ_CP118702             4673 bp    DNA     circular CON 04-APR-2024
DEFINITION  Aeromonas hydrophila strain K522 plasmid pK522-NR, complete
            sequence.
ACCESSION   NZ_CP118702
VERSION     NZ_CP118702.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33408175
            Assembly: GCF_028994095.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas hydrophila
  ORGANISM  Aeromonas hydrophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Luo,X.
  TITLE     The sequence of Aeromonas hydrophila K522
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Luo,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) Department of Clinical Laboratory, Taizhou
            Municipal Hospital, No.381-1, Zhongshan East Road, Jiaojiang,
            Taizhou 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JAN-2023
            Assembly Method                   :: Unicycler v. v0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028994095.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 06:58:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,081
            CDSs (total)                      :: 4,923
            Genes (coding)                    :: 4,828
            CDSs (with protein)               :: 4,828
            Genes (RNA)                       :: 158
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 119
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 15 of 96
            Pseudo Genes (incomplete)         :: 74 of 96
            Pseudo Genes (internal stop)      :: 13 of 96
            Pseudo Genes (multiple problems)  :: 7 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118702.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Aeromonas hydrophila"
                     /mol_type="genomic DNA"
                     /strain="K522"
                     /host="Homo sapiens"
                     /db_xref="taxon:644"
                     /plasmid="pK522-NR"
                     /geo_loc_name="China: Zhejiang"
                     /collection_date="2020"
     gene            8..361
                     /locus_tag="PWO56_RS25315"
                     /old_locus_tag="PWO56_25315"
     CDS             8..361
                     /locus_tag="PWO56_RS25315"
                     /old_locus_tag="PWO56_25315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_270697790.1"
                     /translation="MKFTFAVAKMSKADIGRAQGHNLRLHETSSQLPKRAWFTDRQMYT
                     KVEWDQGKLDTASALAKRKDAVVAIEFIIQVGNQTDWREEPTPECPEGPPIGDGRENGQ
                     NRTLSMRPVRLRP"
                     /name="hypothetical protein"
     gene            817..1512
                     /locus_tag="PWO56_RS25320"
                     /old_locus_tag="PWO56_25320"
     CDS             817..1512
                     /locus_tag="PWO56_RS25320"
                     /old_locus_tag="PWO56_25320"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_270697792.1"
                     /translation="MGQFFTPFGEQVKAWADKEFGPDNVVSIQLHMDESTPHFHVIATP
                     IKDGKLQAKAWLDGGRKLAGIRRRCHATINEKIPCVYTPNGLGGNPHRPDLRAGTAPAP
                     TLIDKMSGHAKAQKLERENAALQEENAQLKQLLFSRQKGRYSADNVELAQQATAEAQKA
                     QSELQKAQGEVKRLTMALDSAQSVIARQGAEIDKLKGYNRTLSEQNNELEEKLQELQPK
                     RSRGKGMGR"
                     /name="plasmid recombination protein"
     gene            complement(1697..1936)
                     /locus_tag="PWO56_RS25325"
                     /old_locus_tag="PWO56_25325"
     CDS             complement(1697..1936)
                     /locus_tag="PWO56_RS25325"
                     /old_locus_tag="PWO56_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015056833.1"
                     /translation="MQEKEAKTVFDMGGFSKAMIVPTPMGSGFHLHLVRFGKAGTEVVE
                     RQRGGWRVFSSIDAAANTAHGIGFRRIEIDLSSR"
                     /name="hypothetical protein"
     gene            complement(2045..2251)
                     /locus_tag="PWO56_RS25330"
                     /old_locus_tag="PWO56_25330"
     CDS             complement(2045..2251)
                     /locus_tag="PWO56_RS25330"
                     /old_locus_tag="PWO56_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017780732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042888847.1"
                     /translation="MGFDKHGIEVDGDCIWLLDAGGQRLCDLTEMQLLDFGRRISVEGG
                     LLNFDLDAAEWRERLIALGLEPH"
                     /name="hypothetical protein"
     gene            complement(3013..3315)
                     /locus_tag="PWO56_RS25335"
                     /old_locus_tag="PWO56_25335"
     CDS             complement(3013..3315)
                     /locus_tag="PWO56_RS25335"
                     /old_locus_tag="PWO56_25335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_042888848.1"
                     /translation="MNSFSLGWEDAALDDRVAIYEFLVEFNLEAAERTDLLIEAAAESL
                     MQHPNRHPACSGGKGRKMVLTKIPFILIYSVDTKINCVRVLRVFHQAQKIPKLDH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3316..3738)
                     /locus_tag="PWO56_RS25340"
                     /old_locus_tag="PWO56_25340"
     CDS             complement(3316..3738)
                     /locus_tag="PWO56_RS25340"
                     /old_locus_tag="PWO56_25340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087757478.1"
                     /translation="MEGFDVAFKSQMESTKVSSRRGSASPLTVSQSYKQSTKAEELSFR
                     ISAKLLADIGLKNGDKADVLFDDETSRWMIRPSQDGFTISGKAEAPTGLIRYTLKVGHA
                     RLTNKRADLPVKKECDIDTLSISERSIIFALQQEGA"
                     /name="hypothetical protein"
     gene            4105..4401
                     /locus_tag="PWO56_RS25345"
                     /old_locus_tag="PWO56_25345"
     CDS             4105..4401
                     /locus_tag="PWO56_RS25345"
                     /old_locus_tag="PWO56_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013783738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042888850.1"
                     /translation="MDARIQFRVSEETKRLAQMYADKNGTTLSDECRKLAEKLAEEQRK
                     QEDHDSWLEEQITAAFTKLAEGRAVFTSHEDAKAKMLARKNAIKGKHIKSLVY"
                     /name="hypothetical protein"
     Typing          complement(1698..1939)
                     /name="rep_cluster_2020"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.9300000000000005e-124"
     Typing          9..295
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.75"
                     /coverage="100.0"
                     /evalue="7.61e-52"
     Typing          800..1509
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.093"
                     /coverage="100.0"
                     /evalue="1.06e-141"
ORIGIN
        1 acccccgatg aagttcacat tcgccgtagc caagatgagc aaagccgaca ttggcagggc
       61 tcagggacat aacctccgcc tccacgaaac cagcagccag cttcccaagc gggcatggtt
      121 tacggatcgt cagatgtaca ccaaggtcga gtgggatcag ggcaaactcg atacagcctc
      181 ggcactggcc aagcgcaaag atgccgtggt ggcgatcgag ttcatcatcc aggtcggcaa
      241 ccaaacagac tggcgggaag agcccacacc ggagtgccct gaagggcctc ccatcgggga
      301 cggtagagaa aacggacaaa atcgtacgct aagcatgcgg cctgtcaggt tgaggccata
      361 gagtttgacg tttaggcgtc agataatgca gaaagcggcc ctagtctgac gcgatgccag
      421 tgagccgact gacgcccagt tgaggtatag cctagggaag gtgcgaataa gtcaccaaac
      481 ataaaaatgc gcccttgtaa gcatttgaat ttaataaact aaatttttca gtttcgttgt
      541 ggagggtgct tattcgcacc ctccctagcg ctctgagcga actggcaaaa cagttttggg
      601 aaaaacgagg ataacgcttt tcagtatctc gacagggata acttacttta ccatcggtta
      661 tcccttttag ccgcacaaat tttagccatg gcttttcagg aagtcagggc tatcagaatg
      721 gccttagaaa gcctagtcaa agagctgtca cgagaacacc gttagcttag cgtacgattt
      781 tttccgaatt ctgcggttcc ccccatcgag cgtgatatgg ggcagttctt caccccgttc
      841 ggggagcagg tcaaggcatg ggccgataag gagttcggtc ccgataacgt ggtatcgatc
      901 cagctgcata tggacgagag caccccgcac ttccatgtga ttgccacccc gatcaaagac
      961 ggcaagctgc aggcgaaagc gtggttggat ggcggccgaa agctggcggg gatccggcgc
     1021 cgatgccacg ccacgatcaa cgaaaagatc ccctgcgtgt acacccccaa cggtctgggg
     1081 ggaaacccgc accggccaga cctgcgcgcc ggcaccgccc cagccccgac cctgatcgac
     1141 aagatgagcg gccacgccaa agcccagaag ctggagcggg agaacgccgc actgcaggag
     1201 gagaatgccc agctcaagca gctcctgttt agccgccaga agggccgcta tagcgcggac
     1261 aacgtggagc tggcccagca ggctaccgca gaggcacaga aggcacagag cgagctccag
     1321 aaggcacagg gggaggttaa gcggctcacc atggcgctcg atagcgccca gagcgtgata
     1381 gccagacagg gggcggagat cgacaagctc aagggctaca accgaacgct ttcggagcag
     1441 aacaacgagc tggaggagaa actgcaagag ctacagccaa agcggagccg cggtaagggg
     1501 atgggccgat gaaggcatgg caggttgtag ggctgatggc agcagtgctg gcagtaaagg
     1561 gggcaagcag ctcatcgagt ggatggatca tggggctgct tggcctgcat gacgtattca
     1621 gctgggcagc ccctgcatgg tgggaagggg cggcatgctt gggggcgctg gccatcctgc
     1681 tgcgatgggc gcgaccctag cggctgctca ggtcgatctc gatgcggcga aagccaatac
     1741 cgtgggccgt gttggcagcc gcatcgatgg aagagaacac ccgccagcct ccgcgctgcc
     1801 gctccacgac ttcggtaccc gccttgccaa agcgcaccaa gtggaggtgg aacccagaac
     1861 ccatgggggt ggggacgatc atcgccttgg agaatccccc catgtcgaac accgtcttag
     1921 cctctttctc ctgcatcatc aaatctccaa tctgagatat caaatagaca atttgatatt
     1981 accccttcgg gagcctaaag caatcaattt ggcgatttga atcatcgaat tcggaaaatg
     2041 attttcaatg cggctcaagg cccaaggcaa tcaggcgttc gcgccattct gctgcgtcca
     2101 aatcgaagtt cagcagcccc ccttcaacgc tgatccgcct cccaaaatcg agcaactgca
     2161 tctccgtcag gtcgcacagg cgctggccgc ctgcgtccag tagccagata cagtccccat
     2221 cgacctcgat cccgtgctta tcgaacccca tacgtacctc cggccagaac caaacccgct
     2281 tccgtggata actgctgccg cccacaagcc gaaccaatgg catgtggacg gacgggcgca
     2341 gttacccacc tcgccaagat catctctctt ttttttgttt gaaagacaga gcgtaaaaca
     2401 caggcgaacc tatctttcgc tgcgcttcaa gataggttcg ccctcctgct tggctacgtt
     2461 aaaaatgacg tagttacgct ggatagctcg ccttgctcag gactattgag gctgcttgcg
     2521 gcagctcccc ctcacgcgct cctgagacac accttgccac tctgcacctg cttcagtccc
     2581 cacctgtggg ccggactgcc gcgccgtttt tatcgagctg gccgtggttg gttccccgtt
     2641 gcgctcggtg ctggtgcccc aaggttgcga accatgggag gctatggtgc gcgtcgcacg
     2701 ctgggccgta tgtgaggatt ccccgttcgg cccgacccct acacccgctc gacaacatca
     2761 acatgcttcc aattcgagcg gatacgacta atcgccattg gcagaggcca acggtaacag
     2821 ggttcgggcg agagactact tgattgatag ggccgtaggc cgctaaactg aaagggtatt
     2881 acggtagctg cttccgcccg aagctgttac caggtacaga gagcgccaac tcaaagtacc
     2941 ttcgaacaaa ccacctgcca gggtggtttt ttcgtttctg gcgaacaggt tacgctgaac
     3001 gcctgaagat cgtcaatgat ctagcttcgg gatcttctgt gcttgatgaa acactcgcag
     3061 cactcgaaca cagttgattt tggtatcaac gctgtaaatc aagataaagg ggatctttgt
     3121 cagaaccatc tttctcccct ttccaccaga gcatgcagga tgccgattgg ggtgctgcat
     3181 caaagactct gcagctgcct caatcagcag atcagtcctc tccgcagcct ccaggttaaa
     3241 ctccaccaga aactcataga tagcgacacg atcatctaac gctgcatctt cccaacctag
     3301 cgagaaactg ttcatttacg ccccttcttg ctgaagagcg aaaatgattg agcgttccga
     3361 tatggacagc gtatcgatat cacattcttt tttgacaggt agatccgctc gcttattcgt
     3421 cagtcgggca tgcccaacct tcagtgtgta ccggatcaac cctgtagggg cttcagcttt
     3481 acccgagata gtgaaaccgt cttgggatgg cctgatcatc caacggctag tctcatcatc
     3541 aaacagaacg tcagctttat cgccattctt cagcccaatg tccgccagca atttagcgga
     3601 tatccggaat gacagctctt cagcctttgt tgattgttta tatgactgag acactgtaag
     3661 tggagaggct gaccctctgc gtgaagatac cttcgtgctt tccatctgac ttttaaacgc
     3721 cacgtcaaaa ccctccattc gatcacatac ctcaattcta ctcccacacc accgatcgat
     3781 caacactcca caacagatcg ccaaccaacc acgaaacgcg ataattaaaa aaatcacata
     3841 aacacaaaaa cactccacac cgcgcgatat cgcacacaac aaaaaatcca taaacgtgac
     3901 aacagaggca tgaataagag aatgagtccg caatgagtcc ttttatgaaa aaaccattta
     3961 aaaacaacaa aataaaaaca acatcagtat gttttcatat gtttttaata ttatttttgt
     4021 gtactacagt aaacaaatgt cagcacatgc gtgctaacat ccgccataga gatccccacc
     4081 agatcaaccc acacgaggcg accaatggat gcaagaattc aattcagagt cagtgaagag
     4141 acaaagcgat tagcccagat gtatgcagat aaaaatggaa ctacgttgag cgacgaatgc
     4201 cgcaagttgg cagaaaagct ggcagaagaa caaagaaagc aagaagacca tgattcatgg
     4261 ctcgaagagc agataacagc agccttcaca aaactggccg aaggaagagc ggtcttcaca
     4321 tcacacgaag acgccaaagc aaaaatgctc gcaagaaaaa atgccattaa gggcaaacac
     4381 atcaaatctc tcgtttactg agagtggggc acaatgcccc accagtttcg catgcgaaat
     4441 tcatagtggg gccagtggcc ccactttcgt aggtagtcag ggatcccccc tcttgttgca
     4501 attacgcttt tcgcggaaac ctcccgaaca gggggaggct agcgcagtct gcctcctcca
     4561 gatcgggagg aggcaagcgt agcgcggcag gtcttttttg ccccgcaggg ctccttcttt
     4621 gcgaagcaaa gtgtaaggag tacactttga actccaaagc cgcaaccctc gaa
//
