LOCUS       NZ_CP118571             5194 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00199 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP118571
VERSION     NZ_CP118571.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907134
            Assembly: GCF_028898905.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00199
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028898905.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 22:06:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,948
            Genes (coding)                    :: 4,841
            CDSs (with protein)               :: 4,841
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 44 of 107
            Pseudo Genes (incomplete)         :: 75 of 107
            Pseudo Genes (internal stop)      :: 11 of 107
            Pseudo Genes (multiple problems)  :: 22 of 107
            Pseudo Genes (short protein)      :: 1 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118571.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00199"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2020-05-06"
     gene            501..737
                     /locus_tag="PXV21_RS25260"
                     /old_locus_tag="PXV21_25260"
     CDS             501..737
                     /locus_tag="PXV21_RS25260"
                     /old_locus_tag="PXV21_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(1239..1883)
                     /locus_tag="PXV21_RS25265"
                     /old_locus_tag="PXV21_25265"
     CDS             complement(1239..1883)
                     /locus_tag="PXV21_RS25265"
                     /old_locus_tag="PXV21_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(1977..>2132)
                     /locus_tag="PXV21_RS25270"
                     /old_locus_tag="PXV21_25270"
                     /pseudo=""
     CDS             complement(1977..>2132)
                     /locus_tag="PXV21_RS25270"
                     /old_locus_tag="PXV21_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            3147..3365
                     /locus_tag="PXV21_RS25275"
                     /old_locus_tag="PXV21_25275"
     CDS             3147..3365
                     /locus_tag="PXV21_RS25275"
                     /old_locus_tag="PXV21_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(3656..3841)
                     /locus_tag="PXV21_RS25280"
                     /old_locus_tag="PXV21_25280"
     CDS             complement(3656..3841)
                     /locus_tag="PXV21_RS25280"
                     /old_locus_tag="PXV21_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4207..4434)
                     /locus_tag="PXV21_RS25285"
                     /old_locus_tag="PXV21_25285"
     CDS             complement(4207..4434)
                     /locus_tag="PXV21_RS25285"
                     /old_locus_tag="PXV21_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     ARG             complement(1243..1883)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          163..336
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          3522..3609
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(5..190)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.513"
                     /coverage="99.0"
                     /evalue="4.15e-69"
     Typing          complement(2392..2883)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.093"
                     /coverage="88.0"
                     /evalue="0.0"
     Typing          complement(4670..5176)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.774"
                     /coverage="99.0"
                     /evalue="6.33e-132"
ORIGIN
        1 tcatgatgtt tgtcaggggg gcggagccta tggaaaaacg gcttcgccgc ggccttatct
       61 cttccctgtt aagctccttc ctggtatctc ccaggaaatt tccgccccgc ctgtaagccg
      121 gcaccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaat
      181 atatcctgta ctacatatcc tgctgacgcg ctggtgccgc cttttttctc ctgctacatg
      241 aagcacttca ctgatttcca catccgtgcc aacatagtca gccagtatac actccgctag
      301 cgctacgtga ctggttcagg gctgcgcccc gaaaccggct aaaagctaat gacgcgcctg
      361 cggcttgtcc aacgcctcac ggcaccggat taaaatccgc tcccgttcac cgtttctgac
      421 tgcctttcac cgtcgctggc gcttcatcgt ttctactgtg tctggcagac tgtgctgatt
      481 tattctgact ggagaacgtt atggccagac tgacaaactt gacgcctgca gagaaaaaat
      541 ttcttgatga tgctgttgcc gcagctgaaa gagcgtcagg taaaaagcta aatcagccta
      601 accggcacat cgtcctgaac agagcgcgtg cgcaaattga gttgcagcgt tacgccgacc
      661 gccagcgtgc attacgtgag gatgagcggc agcagtccga ttttgcctgg tcacgtccgc
      721 gcgcgccgcg ccgctgattt tggcttttgg cggtttagtg gtgctgacgc tgcgaaatca
      781 cctgccattc acctgcgtgc atagccatgc agtcgcgcag aaatcatcag gggcgttttt
      841 ccgggatact ggtgaggaaa ggtgctggaa acggccctga tgcagcagga ttgcgctgag
      901 ggcgcctgcg ggggtgcggg caagggatgt tatgtttaat cggtctgacc gtcaggtaca
      961 aaaaaaagcc cttagggggc tttttttttg ctatgagtta gccatatttt cgttaagaca
     1021 tggcttcatg attcgggagt ggttattcgt tgttctcgcg aaaatgaatt ggttagatat
     1081 tcatcaaatt cctgtccaga atgcagcatt agatggcatg tcgttgatgc ggtaggtcag
     1141 gatattcgtc cactggagaa aacgtttcac aatgcttgcc aggagtcgta ctggttaaaa
     1201 gtaaatcgtc aaacgcatct cccggtgtaa acgctgaact aaccaatcac agcgatgcca
     1261 agccgctcca tgagcaacga tgcctggtag ctgtctaact taacgccttg taaatcaaca
     1321 ccccgaatat ctaagtcacc taactccgaa ttggtcagat cgcaatgtgt gaagtttgct
     1381 gctcgccagt cgaaagtcga aaactcgccg ccggagagat ctgaaccact gaacgtcgca
     1441 cccagtacct gagtccccat ccagcggttt tcccacagct cacacttttc caacacgact
     1501 ttcgaaaaat tggcgtagct tagattagta ttagtgatat atgcgctgca aaaccaggtg
     1561 cgcgtggtga tcatattcat aaagcttgca ccgcggaaat ctgcgccttg tgcgcggcag
     1621 tggcgaattt caatgcccaa ggcactgacg ttgcggaaat ctgccattga taaatcacag
     1681 cttttgaaaa tggcatcttt cagcattgcg cgactaaaat tgcacccttt ctgactttcg
     1741 cgatcataga actggcagcc gataaattca gtgccgctca ggtcggcacc tgaaaaatcg
     1801 cagttaaaaa atgtactatt ttcaactttc tcaccggtga agcgatttct gtcaattttt
     1861 tcgccaacta atgccagagt catatctatg cctgtgtttt tatacagtaa tgacgccatg
     1921 gtaaacctta tgacgttatg cgtcaaaccc gccagtatga cacctgcaaa tgcgtattag
     1981 atctggtgtt tcttcagcaa ggcacgtagc tgatgataag tcagacccag taattcagcg
     2041 gcttttttct ggttaaattt agcctgctgt aagctggttt gtagaaagtc tttctcttgc
     2101 tgctgctgga attcacgcag atccagcgtt ataacgatgt gtatcctatg tgctcggatt
     2161 ggatagattt ccattacggt gaaggatctt cttgagatcc tttctgtggc gtcgtaatct
     2221 cttgctctgt aaacgaaaaa accgccctgc aaggcggttt ttcgaaggtt ctcagagcag
     2281 caactctttg aaccgtggta actggcttac gaggagcgca gtaaccaaat ctgttctttc
     2341 agtttagcct taacaggcgc ataacttcaa gactaactcc tctaaatcag ttaccagtgg
     2401 ctgctgccag tggcgataag tcgtgtcttt ccgggttgga ctcaagatga tagttaccgg
     2461 ataaggcgca gcagtcggac tgaacggggg gttcgtgcat acagtccatc caggagcgaa
     2521 ctgccttcgc ggaactgagt gtcaggcgtg gaatgagata aacgcggcca taacagcgga
     2581 atgacaccgg taaaccgaaa ggcaggaatg cgggggagca cgagggagcc accaggggga
     2641 aacgcctggt atctttaagc cgcatcgggt ttcgccacca ctgatttgag cgtcagattc
     2701 tgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttggcgcg gccttatgct
     2761 ttcttcgtta agtatcttcc tggcatctcc caggaaattt ctgatccatc cgtaagcccg
     2821 tcccgctcgc cgcagccgaa cgaccgagcg gagcgagtca gtgagcgagg aagcggaata
     2881 tattctgtat cacattttct cctgacgcgt tttctttcac tttctgcgcc tgtcttatgt
     2941 ggcattaatg ctatgtgtta ctgccatgct acatcttaag ccagtataca ctccgctagt
     3001 gctccgtgac tggtccggcg ctgcgcccgg aacccgcctg taccggttca gcagccgttc
     3061 cggcctgact gcaatttttt ttttttcatc cctgcccgct accctgtaaa cctttcttct
     3121 gcgttgccgt taacagtagg taagccatgt ttggtttttc ttccggagcc ctgatgactc
     3181 ttagtgatga tgagcgacac ctgcttgtgt ccgttgtttc ggtctggcta cggcgtgccg
     3241 gaggggatgc cggagccatg atgctggatg cataccggca gattttgtct gaaaccgaac
     3301 cggccgtaag gactgtcatg ctcgaatttc ttgaatccgt tcgcattcat tacatttcgt
     3361 cctgagcatt ttctgtattt ctgtccatac ttaaacgagg cggccgtcac ggacggtggt
     3421 cgtcgttcca tgatgctgtt atggacgagt gagagtaatc taatctaact tgattgagtt
     3481 ccgggcgtgc cgcccggttt tttttgctgt ttcccggtgt ccgttccggg acaggatgtg
     3541 catttgtaat accgcgcacg cgccggcatc acaaataccc tggtcagggc tgcgccccga
     3601 caccccgcca tcgcctcacg gccgcgcact gaattgcgca cccgttcacc gattttcacg
     3661 cattttcctc gcctatctgt gcgttcagca tggcgtaaag cgcttcggcc tgatcgtgca
     3721 ggtgctcaca gcagccggca gcctcatcca gatccatctg ctcgagccgc tcaaggagga
     3781 gaagtgtctg tgcgcggata aatttggcca tattgagggc ggcggattcc tgtctggtca
     3841 ctggtgattt tccccgtctg gtgatgattt ttgcgtgaaa agagcatggc gtcattctgg
     3901 tgcatagtca tgcatgccgt taaaatttaa caggcgcgtt tctgacgggt tccggggtgg
     3961 tttgttgtgg tttttgtcat ggtttcgtca ggaacgcgct gaacgcgtct caggcggtgc
     4021 gggcaacgga tgttatggta aattcttggg ggcttgatta taaaataccg cacttcctct
     4081 tatgtcttgt gctttttaga caaccctgaa agcttttagc tttggtgatg caatttgctg
     4141 ggattttggc ggagaaccac aggtaaagaa aaaggccaca ttagcggcct ttttcggaga
     4201 agatgctcag tgcggaattt cggagacttc tttaacgtcg gttttattga tctgctgttt
     4261 gacaccattc gcgtctgtat agccaagcag gcctgagtct gattctttag gtttgccgtc
     4321 gctgacaata gtgcgaccgt cattggtgtg tatggcatag cttgtgcgcg tgcagccggt
     4381 gagggctgaa agcgcaactg ccgctgcgaa cactgaaaca aaaatctttt tcaaagccag
     4441 ctccttttat ccatatcagt tttggtcact aaccatctaa ctataggtca ttttttatgt
     4501 gaaaagaggc ttgatggtgg gctgtatatc gagttaggcg cgaggtgcta tggtcaaagt
     4561 gtggtgtcag gcggggagga ggcttatatg agatgtaatc cggccgatac atataccaag
     4621 atgatttaag tattcctctt tgtgaggttg gatgtctaat ttataaagga tcttcttgag
     4681 atcctttttt tccgcgcgta atctcttgcc ctgtaaacga aaaaaccacc ctggcaggtg
     4741 gtttttcgaa ggttaggtaa tcctggcaga tcccctaacc gtggtaacag tcttgtgcga
     4801 gacatgtcac caaatttgtc ctttcagtgt agcctcacta aggccgccac ttcaagaact
     4861 cttgagacat ctctcgcaca tcctgtttgc caatggccgt tgccaatggc gattagtcgt
     4921 gtctttccgg gttggactca agttgatagt taccggataa ggcgcagcgg tcggactgaa
     4981 cggggggttc gtgcatacag tccatcctgg agcgaactgc cttcccggaa ctgagtgtca
     5041 ggcgtggaat gagaaaccgc ggccataaca gcggagtgac accggtaaac cgaaaggcag
     5101 gaatgcgggg gagcacgagg gagccaccag ggggaaacgc ctggtatctt taagccgcat
     5161 cgggtttcgc caccactgat ttgagcgtca gatt
//
