LOCUS       NZ_CP118556             4665 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00206 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP118556
VERSION     NZ_CP118556.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907141
            Assembly: GCF_028891505.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00206
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease Submission Group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028891505.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 07:07:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,070
            CDSs (total)                      :: 4,955
            Genes (coding)                    :: 4,867
            CDSs (with protein)               :: 4,867
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 36 of 88
            Pseudo Genes (incomplete)         :: 52 of 88
            Pseudo Genes (internal stop)      :: 18 of 88
            Pseudo Genes (multiple problems)  :: 17 of 88
            Pseudo Genes (short protein)      :: 1 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118556.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00206"
                     /isolation_source="other"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2020-06-03"
     gene            complement(160..405)
                     /locus_tag="PWP98_RS25285"
                     /old_locus_tag="PWP98_25285"
     CDS             complement(160..405)
                     /locus_tag="PWP98_RS25285"
                     /old_locus_tag="PWP98_25285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050497065.1"
                     /translation="MQPQSAVHDGLPGIDAWTVGAIVIRLEPSAGQQLRVRDQKIQLKP
                     PLVTMLHPQDAVLVFIKSGHQNPLKACHQLFPLAGR"
                     /name="hypothetical protein"
     gene            656..1141
                     /locus_tag="PWP98_RS25290"
                     /old_locus_tag="PWP98_25290"
     CDS             656..1141
                     /locus_tag="PWP98_RS25290"
                     /old_locus_tag="PWP98_25290"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012565228.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1145..1360
                     /locus_tag="PWP98_RS25295"
                     /old_locus_tag="PWP98_25295"
     CDS             1145..1360
                     /locus_tag="PWP98_RS25295"
                     /old_locus_tag="PWP98_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_012565230.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMLRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1706..3262
                     /locus_tag="PWP98_RS25300"
                     /old_locus_tag="PWP98_25300"
     CDS             1706..3262
                     /locus_tag="PWP98_RS25300"
                     /old_locus_tag="PWP98_25300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589620.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_032993376.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVLV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPLKSVTGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHNLFGDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTYIKVMMERVDFLNEVALKDPLTGVNNRLGFLSKIQNHKRDSGYSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            4320..4643
                     /locus_tag="PWP98_RS25305"
                     /old_locus_tag="PWP98_25305"
     CDS             4320..4643
                     /locus_tag="PWP98_RS25305"
                     /old_locus_tag="PWP98_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4034..4207
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(3558..4110)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.741"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          2..1464
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.984"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          4321..4640
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.458"
                     /coverage="100.0"
                     /evalue="3.26e-62"
ORIGIN
        1 ggtggcgccg ggccggtgga ctatctgctg ggtaaagacc ggcagcgtga aggcgccacc
       61 gtcctgcagg ggaagccgga agaagtccgg gagctgattg acgcgtcgcc ttacgttaag
      121 aaatacacct ccggggtact gtcgtttgcg gaagcggatt taccttccgg ccagcgggaa
      181 aagctgatgg caagctttga gcgggttctg atgcccggac ttgataaaga ccagtacagc
      241 atcctgtggg tggagcatcg tgacaagggg cggcttgagc tgaattttct gatcccgaac
      301 acggagctgc tgaccggcag acggctccag ccgtattacg atcgcgccga ccgtccacgc
      361 atcgatgcct ggcagaccat cgtgaacggc agactggggc tgcacgaccc gaacgcaccg
      421 gagaaccggc gggcactggt cacaccgtct gcactgccta aagcgaagca ggaggctgca
      481 gaagcgatta cacgcggtct gcttgctctt gcctcatccg gggagctgaa aacccgtcag
      541 gatgtcactg aggcgctgga aagcgcaggt tttgaggtgg tccgcaccac aaaaagcagt
      601 atcagcattg ccgacccgga cggggggcga aacatccgac tgaagggagc catttatgaa
      661 cagtctttta acgctggcga aggacttaga gcagaaatcg aaagcgcagc agcagaatac
      721 cggcgagatg ctgaaagccg cattcagcga gcacgagaaa tctgtaaaag cggaactgaa
      781 cgaaagcgcg aagagaatca gcgccgccat cctcgaccac gaccggaaac tgtcctcagc
      841 catgagccag cgcacgaaag gcatggtgcg catggtcagc cagacgtggc tgaccatcgt
      901 cctggtctcc gcgctgctga tcgcctcgag cgcgggcatt ctgtggtggc aggggcagca
      961 gatactcgac aactacacga ccatccggga gcagaagagc acgcaggcca tgctgtcaga
     1021 gaggaacagc ggcgtgcagc tctcgacctg cggcgagcag agacgccgct gcgtgaaggt
     1081 gaacccggaa gcgggacggt tcggagagga ttcgagctgg atgatactgg cggggaaata
     1141 gcacatgacg gagctggaaa aacagttgct gagcgcatta gagcagctac agcaggacta
     1201 ctcgaaaagg ctggacgagt gggagagcgc cttcgcggaa tggcggacga tgtctggtct
     1261 tatgctacgg gagaacgcag cgctgagcga gcgcgtcacg ggcttgagca ggcaggtgca
     1321 gagtttgagc gagcagctgc gccggttgtc gagcgactga atgccgtcga gctgcaccgg
     1381 gagcaggagc gcgtggtgca gcatcagaaa gcgctggagc tggagcgatc tcagcgacag
     1441 cggacgtata acagcccttc actgtgataa cctgataaaa cggggctttc ggcggacatc
     1501 tgcgctgaaa gccggagcca ttcacccgac tgcatagcca tgcagccggg tgaaatttat
     1561 ctggcgcaga atgcgcggtt tagcggagag tttgctgccg gttttaccgg gctgacgccg
     1621 catttgttcc gaacccgtcc ggggcggtgc tgataaggga tgttatggta aatgcggcat
     1681 tttttattat atggagtgta tttttatgag aattaaaaag cctcaccttc gtccgatagt
     1741 cataaccttg tccgttggtg ggattttgtt aacttctatt tttctgattg ccgttattat
     1801 ggtttttcag cgcgaaaata tagaaaaaga tttgctttat gctaattcat cgtacgctat
     1861 gaaaatgtca gatgtaatga gtggttatat tgaaatggct caaggtgaat tagcatatgg
     1921 agcaaaaaaa ataaaatcaa ctaatgatat tgactcactt aaagatgagg cggataggtt
     1981 aagattgcag tctggtatgt ttaattcagt tcttgttgtt agtaatgatg ctgttgttct
     2041 ggcgacttcg ccagaatctc ttgatttagt tggtacccgc ttgaattcaa gtgccagcaa
     2101 attggcgatt gaggaaaaga aaccattcat atcttcaccg ttaaaatcag ttactgggaa
     2161 tttgattatt ttgttgtcac atcctattta tgataagggt ggcaattaca tagggtatgt
     2221 cggtgggtct atatatctaa aaaaacataa tttatttggc gatgttttga gcaggcattt
     2281 tttcaagaac aacactgaaa ttagcattgt tagtgatgat gggaatgtga tctttaataa
     2341 cgataaaagt gctgttgatc ggcctatggt tatgtctgat gatttgaaat caaagttaac
     2401 tgcgtctgat agaggtgagg gggcattttt ttcttcgggg catagatatc ttcttggtta
     2461 tgctagtatg aaaaatactg cttggaatgt gttggtttat agtcgtgctg acagtgtgac
     2521 tactattctt attgcttatg ctaaaagtgt acttgttctg ctaattataa taattattgt
     2581 gttttcactt atttcatatt tcattgcctc cagtatttca cttcctttag aaaagcttgc
     2641 tatatctacc aatgctaaag atatcggagc ttcgctagtt tatattaaag ctatcaatgc
     2701 ttggtatgcc gaagctgata ggcttaaaaa ggcgcttttt acgtatatta aggtaatgat
     2761 ggaaagagtt gattttctaa atgaggtggc gcttaaagat ccccttaccg gcgtgaacaa
     2821 tagattgggg tttttaagta aaatacagaa tcataagcgg gattcagggt attcagttat
     2881 tgtaattgat gttgattttt ttaaagagat aaatgacagt ttcgggcatg gggtcggaga
     2941 tgaggtgctt gtttctttgg ctcatattat taattcctgt tgccgtagtg aagatgttgt
     3001 ttgtcggttt ggtggcgaag agtttgttgt tttcctgccg aatacatctg tggtaacagc
     3061 taagtgtgtt gcggagcgta ttaggagtgt tattgaaagt actgtcttcc ctaataactt
     3121 gcgggtcacc atatctgctg gtgtggcaac acaagatgat cctcttggta ggatcgattt
     3181 tttgctgaaa aatgctgacg atgctcttta tcaagccaaa agggaggggc gtaataaagt
     3241 gattgtatat agcgctattt gagttttttg atgatgcttt acgcgctagt gtgtcgtttt
     3301 ttttgtggcg aagagtgggc aagaagcgga tcctcttgtt atcccttcga tgtcgtcgta
     3361 atctcatacc ctgtaaacaa aaaaaccacc gctaccaacg gtggttcgtt tgccggaatt
     3421 cagagctacc aactctttct tccgaaggta actggctttc acggagcgca ggtaccaaaa
     3481 ctgtccttcc agtgtagccg cagtcaggcc accacttcaa gaactctgta gaacactcta
     3541 ctactccgct ccgtgaatcc tcttactaat ggctaccgcc actggcgatt tttcgtgtct
     3601 gttccgggtt ggactcaaga tgatagttac cgggacaacg tggtagtcgg gctgaacggg
     3661 gggttcgtgc atacagtcca gcttggagcg aactgccttc ccggaaccga gtgtcaggcg
     3721 tggaatgaga taaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     3781 caggagagcg cacgagggag ccaccagggg gaaacgcctg gtatctttaa gtcctgtcgg
     3841 gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct
     3901 atggaaaaac ggcttcgccg cggccttctc gcttccctgt tacgttcctt cctggcatct
     3961 tccaggaaat ttccgcccca cctgtaagcc ggcaccgctc gccgcagccg aacgaccgag
     4021 cgcagcgagt cagtgagcga ggaagcggaa tatatcctgt atcacatatt ctgctgacgc
     4081 gccggtgccg ccttttttct cctgccacat gaagcacttc actgacaccc tcatccgtgc
     4141 caacatatta agccagtata cactccgcta gcgctacgtg actggttcag ggctgcgccc
     4201 cgaaacccgc caaaagcctg acgcgcctgc ggcttgtcca accccgtgcc gaccgggaaa
     4261 ggttttcccg cccgtcccgg cgttatcagg agaccggttt gtcagagaaa cggaacaaga
     4321 tgctcaccat gtgggtgacg gaggacgagc accggcggct gcttgagcgc tgtgacggca
     4381 ggcagctggc ggcgtggatg cggcagacct gcctggacga aaagccggca cgttccggaa
     4441 agctcccctc aatctcgccg gcgctgctgc gccagcttgc cggcatgggc aataacctga
     4501 accagatagc ccgccgtgtt aacgccggcg gcggtaccgg ccatgacagg gtgcagattg
     4561 ttgcggcgct gatggccatc gatgccggac ttgagcggct gcggcacgcc gttcttgaga
     4621 aaggaacgga cgatgatcgt taaatttcat ccacgcggtc gcggc
//
