LOCUS       NZ_CP118411             6210 bp    DNA     circular CON 18-SEP-2023
DEFINITION  Escherichia coli strain GABEEC10 plasmid pGABEEC10_4, complete
            sequence.
ACCESSION   NZ_CP118411
VERSION     NZ_CP118411.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33321545
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   McDonagh,F., McEvoy,N., O'Connor,A., Lonappan,A.M., Hallahan,B. and
            Miliotis,G.
  TITLE     Complete genome sequence of E.coli ST127 strain GABEEC10 identified
            in human saliva
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   McDonagh,F., McEvoy,N., O'Connor,A., Lonappan,A.M., Hallahan,B. and
            Miliotis,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2023) Bacteriology, Medicine, University of
            Galway, University Road, Galway H91 TK33, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                :: 31-JAN-2023
            Assembly Method              :: Unicycler v. 0.5.0
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 681.0x
            Sequencing Technology        :: DNBseq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 09/18/2023 06:28:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118411.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GABEEC10"
                     /isolation_source="Unstimulated saliva"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pGABEEC10_4"
                     /geo_loc_name="Ireland: Galway"
                     /collection_date="2022-05-05"
     gene            28..963
                     /locus_tag="PVF01_RS00005"
                     /old_locus_tag="PVF01_24795"
     CDS             28..963
                     /locus_tag="PVF01_RS00005"
                     /old_locus_tag="PVF01_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_021553019.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLGELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(1158..1556)
                     /locus_tag="PVF01_RS00010"
                     /old_locus_tag="PVF01_24800"
     CDS             complement(1158..1556)
                     /locus_tag="PVF01_RS00010"
                     /old_locus_tag="PVF01_24800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021553020.1"
                     /translation="MRISNVEWLKKRIGFIRKLGEQTARQRQIIDLLDNESGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILHALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1734..2660
                     /locus_tag="PVF01_RS00015"
                     /old_locus_tag="PVF01_24805"
     CDS             1734..2660
                     /locus_tag="PVF01_RS00015"
                     /old_locus_tag="PVF01_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_021553015.1"
                     /translation="MATYHLSVKFGGKGNAANHADYIERKGEYAKRKDLEYTEHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERDFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSSHLEQARTVGHDIGEWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2663..3196
                     /locus_tag="PVF01_RS00020"
                     /old_locus_tag="PVF01_24810"
     CDS             2663..3196
                     /locus_tag="PVF01_RS00020"
                     /old_locus_tag="PVF01_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021553014.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISEGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            3417..5177
                     /locus_tag="PVF01_RS00025"
                     /old_locus_tag="PVF01_24815"
     CDS             3417..5177
                     /locus_tag="PVF01_RS00025"
                     /old_locus_tag="PVF01_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_021554946.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGNGNGGGNGNSSGGGNNSSVAAPVAFGFPALAAPGAGTLGIAVS
                     GEALSAAIADIFAALKGPFKFSAWGIALYGILPAEIAKDDPNMMSKIVTSLPAETVTDA
                     QVSSLPLDQATVSVTKRVTDVVKDERQHIAVVSGVPMSVPVVDAKPTNTPGVFSASFPG
                     VPSLTVSTVNDAPVSTSLPAGVTEDKDRTAFPAGFTFGGGSHEAIIRFPADSGQKPVYV
                     SVTDVLTPEQVKQRQDEDSRRQQEWDARHPVEVAEREYESAKAELEAENKKVQSFQATL
                     DGLKNTPEGLALSDAGQHPLTSSESRFVAVPGYSGGGVHFDATATVDSRDRLNNLINMG
                     ATAYVNNVLELGDVSAPTEDGVKVGNAIKNATIEVYDKLRQRLLDRQNEINNAQASLNA
                     AVESRNQKEENKRDAEGKLNDELEKNKGKIPDLKIDQKIRDQMPERGWTEDDIKNTVSN
                     GATGTSFDKRSPKKTPPDYLGRNDPATVYGSPGKYVVVNDRTGEVTQISDKTDPGWVDD
                     SRIQWGNKNG"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5170..5499
                     /locus_tag="PVF01_RS00030"
                     /old_locus_tag="PVF01_24820"
     CDS             5170..5499
                     /locus_tag="PVF01_RS00030"
                     /old_locus_tag="PVF01_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5 immunity protein"
                     /protein_id="WP_021553017.1"
                     /translation="MANKLFEHTVLYDSGDAFFELKGNASMKLSPKAAIEVCNEAEEKG
                     LWILGIDGGHWLNPGFRIDSSASWTYDMPEEYKSKIPENNRLAIENIKDDIENGYTAFI
                     ITLKM"
                     /name="colicin E5 immunity protein"
     gene            5597..5740
                     /locus_tag="PVF01_RS00035"
                     /old_locus_tag="PVF01_24825"
     CDS             5597..5740
                     /locus_tag="PVF01_RS00035"
                     /old_locus_tag="PVF01_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_021553018.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAELTGVE
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..6210
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          29..963
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.158"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1735..2657
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.13"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcataaccc ggagagaatc gtaacacatg agcgccgcgc ttcaatactt cgacgaaaat
       61 ttaccccacc gtccctatca cacggatgat ctcgcttttg gtcttcgcat ctccggcaaa
      121 gggcgtgcgc ttcttgcgcg gtacatacag cagaaccagc ctcatgcgca gttctggctg
      181 gtttttgacg ttgaccgcga gggggcagcg attgactgga gtgaccggaa cgctcccgcg
      241 ccgaacatca ccgtaaaaaa tcctgtgaac ggtcatgctc acctgctcta cgcgctcaat
      301 atcgccgtga gaaccgcgcc ggacagctcg gttaaggcgc tgaaatacgc cgctgcgatt
      361 gaacgttcgc tgtgtgagaa actttgtgct gatgtgaact acagcggact gatttgtaaa
      421 aatccgttcc acctggaatg gcaggtgatg gagtggcgag aggaagccta taccctcggt
      481 gaactggctg attatctcga tttgagcgct tcagcgcgcc gtagcatcga taaacattac
      541 gggatggggc gaaactgcca tctgttcgaa atgacgcgca aatgggctta cagggcgatt
      601 cgtcagggct ggccagcatt ctcacagtgg cttgatgccg tgattcagcg tgtcgaaatg
      661 tacaacgcat cgcttcccgt tccgctttca cctcctgaat gtcgggctat tggcaagagt
      721 attgcgaaat acacgcacag gaacttcacg ccggaaactt tcgcacagta tgtggctgat
      781 acgcacacgc cagaaataca ggccaagaga ggcaggaaag gtggcatcgc taaaggcgaa
      841 gcctacgatg acaagcgttt catggcgcta tgtatgctgg agaatggata ttctcagaaa
      901 gctattgcgg cgatgttgga ggtttctact cgaaccattc gaaactggaa aagcggaaaa
      961 tagcctatat cagataacag cgcctttctg gcgttttttt gagccgaagg tttttttgcc
     1021 gtttttcccc gttcagtcct gcaatggtgc tgttttccgc atgattgcgc tggtagtgct
     1081 cgccgcagtc tcatgaccga gcgtagcgag cgaatgagcg aggaagcacc agggaaccgg
     1141 aacacggatg gaggcactta cgcgtctggg tttggggatg tctccagatc gtacttaatt
     1201 tccttaagtg catgtaatat cctgtctttt ttatagcata gctcgcctgt ttttgtgtct
     1261 accagtcctg cttcgatcag tagcccagcc gcgagaaaga gtcggtgatt gcgttctcgt
     1321 cgttgttttt ttccctcgat tcgtttttgg accgcctgtt ttcgttcgct ctcctgtgcc
     1381 tgtaagtcgt ttttttccgc tgttgccagt acgtggagaa gtttgcgttc ctgttctgtg
     1441 agtccggatt cgttgtctag caggtcaata atctgtctct gtcgcgcggt ctgttctccg
     1501 agcttcctga tgaatccgat ccgtttcttc agccattcga cgttgctgat gcgcatgttt
     1561 gtggtactcc ctggtgtttt ttttacggcg gtttgtggtc gtgcagtata tcgcttgcgg
     1621 tcggcgggtg caggggttat cttccgtact aattcgaata attagaagga agcgcactta
     1681 tatgtttctc tccttcgtcg agaaacagtg cgctcctgcg gagggcgggg gtaatggcaa
     1741 cctaccactt aagtgttaaa tttggcggta agggaaatgc tgcaaaccac gccgattaca
     1801 tcgagcgcaa aggggagtat gcgaaacgga aagatctgga atacacggag cacggaaaca
     1861 tgcctgaatg ggccagggat aatccttccc atttctggca ggcggctgac cagtttgaac
     1921 gtgcgaacgg ttcgacttat cgggaactgg aaatcgcgct gccccgtgaa ctgacaccgg
     1981 aacagcgcct tgaactggtg caggattttg tacgtcagga agccggcgaa cggcatgcct
     2041 ggagttttgc cattcacaat ccgaaagcga gcattgacgg cggcgaacag cctcatgcgc
     2101 atatcatgat gagccagcgt gtgaatgatg gcattgaccg tacaccggaa cagtatttcc
     2161 ggcgctataa cgccaggtat cccgagcggg gaggtgcgaa aaaggattcc ggaagcctga
     2221 ctcccactca gcaaaaagag cagttgcggg aattgcgaaa acgctgggag gtgaagcaca
     2281 acgaacatat gagaaagcat ggctttgaaa gggacttcat tgactgccgg acgctgaaag
     2341 aacagggcat tgaacgacgt cccgaagtgc atctgggttt tgaagctgcc ggacggcttg
     2401 atgatgccca gcgtcatgac atcatgcaaa aacggtctga accggactat tccagccatc
     2461 ttgaacaggc gcgaacggtc gggcatgaca tcggggaatg gctggacact ccggttaagc
     2521 aacatgaact ggtcagaagg ctgcaacatg atgttgcgcg ggagcatggc tttgacatga
     2581 gagacagagt gagcagacag aaccgaactg aacctgaacg ggttaaatca ccgtcacgtg
     2641 gattttctat gggattctga acatggccag cacagaagaa aaattactca cgctcctgtt
     2701 atcggtggag catctgcaac ataccgcaat ggagcaacag aaagctgttg ctgattctct
     2761 ggctgtggca agggaaacac ttcgcggata tcgggatatt tctgaaggtg tggcaaaaca
     2821 ggtcagggat gaagtcagaa atgaggtgca gaagctggac gtggcagggc tgataggtga
     2881 gcgcatcagc caggattttc agcggctgga agagtcagtc gcaaacatga aggcaaacgc
     2941 ggatgccctg aacagggata ttgcgacaat tcacagcaat atcgtgcagg aatacaacac
     3001 ccttaaaggg tatagctgga aatggctggg tggggtgtca gtggcttttc tggttatgtt
     3061 ctgtgtgctc ggatggggaa tgaaaacaat actcgacagg aactacaacg ccacagcggc
     3121 ggtgtatcag aaagttgtga cactggaaga taaactggcg gagaaacctg tcacgggtaa
     3181 gagccggaaa aactgacgcg cttcgcttgt ccaacgcctc acgacccgcg aaaggatttc
     3241 gcgttccgtt caccgttttc atgagtgatt cctccgaact gccaaaaaat atccccccct
     3301 gacttgacag aaaaaataat ggcgagtact ttttgatctg tacataaaac cagtggtttt
     3361 atgtacagta ttaactatgt aatcaattgt tttaacgctt aaaagaggga atttttatga
     3421 gcggtggaga tggacgcgac cacaacagtg gcgcacataa cactggtggt aacattaatg
     3481 gcggccctac ggggcttggt ggaaatggtg gggcttctga cggttcagga tggagttcgg
     3541 aaaataaccc atggggtggt ggttccggta gtggtgttca ctggggaggt ggctccggta
     3601 atggcaatgg aggggggaat ggtaattcca gtggtggagg caataattca tccgtagcag
     3661 caccagtggc atttggtttt cctgctttgg cagctcctgg tgccggaaca ctgggtatcg
     3721 ctgtctctgg tgaggcttta tctgcggcaa tagcggatat ctttgcagcc ctgaaaggcc
     3781 cgtttaaatt cagtgcatgg ggtattgcgc tttacggcat tctgccagct gaaatagcaa
     3841 aagatgaccc gaacatgatg tcaaaaatcg tgacgtcatt accggcagaa acggtgacag
     3901 atgcgcaggt cagttctctg ccactggacc aggcaactgt cagcgtgacg aaacgtgtga
     3961 cggatgttgt gaaggacgaa cgacagcata ttgccgttgt ttcaggtgta ccaatgagtg
     4021 tacctgttgt ggatgcaaaa ccaacaaaca ctccgggtgt attcagcgca tcatttcctg
     4081 gtgttccttc cctgactgtc agcactgtca acgatgcgcc tgtatcaacc tcgcttcccg
     4141 ccggagttac ggaggataaa gaccggacgg cttttcctgc aggatttacc ttcggtggtg
     4201 gctctcatga ggcgattatc cgcttccctg ctgacagtgg tcagaaacca gtgtatgtgt
     4261 cggtaaccga cgtcctgact cctgagcagg taaaacagcg tcaggatgag gacagtcgtc
     4321 gccagcagga atgggatgcc cgacatcctg ttgaagtggc ggaacgtgag tatgaaagtg
     4381 caaaagcaga actggaggca gaaaacaaga aagttcagag ttttcaggca acgcttgatg
     4441 ggctgaaaaa tactccggaa ggtcttgctc tgtctgatgc aggacaacac ccgttaacat
     4501 ccagtgaatc ccggtttgta gctgtccccg gatacagtgg gggtggtgta cattttgacg
     4561 ccacagcaac agttgattcg cgtgacaggc tgaacaatct gataaatatg ggggcaaccg
     4621 cttatgttaa taacgttctt gaacttggag acgtcagtgc ccctacagag gatggggtaa
     4681 aagtcggtaa tgcaataaaa aatgcaacta ttgaggtata tgacaaatta cggcagcgcc
     4741 tccttgaccg acagaatgaa ataaataacg cccaggcttc tcttaatgct gcagttgaga
     4801 gtcgtaacca aaaagaggag aataaaagag atgctgaagg caaactaaat gatgagttag
     4861 aaaaaaacaa agggaagata cctgatctta aaatagatca gaaaattagg gatcaaatgc
     4921 cagaaagagg atggacagaa gatgatatca aaaataccgt atctaacggt gcgactggta
     4981 catcttttga taagcgcagt ccgaagaaaa cacctccaga ctatttaggt cgaaatgatc
     5041 cagcaacagt atatggttcg ccaggtaaat atgttgttgt caatgatcgg actggtgagg
     5101 tcactcagat tagtgataag acggatcctg gctgggtgga cgattcgaga attcaatggg
     5161 gaaataaaaa tggctaataa attatttgaa catacggtgt tatatgatag tggtgatgcc
     5221 ttttttgaat taaaaggaaa tgcttctatg aagttatcac caaaagctgc aatagaagtt
     5281 tgtaatgaag cagaggaaaa aggtttatgg attttgggta ttgatggtgg gcattggctg
     5341 aatcctggat tcaggataga tagttcagca tcatggacat atgatatgcc ggaggaatac
     5401 aaatcaaaaa ttcctgaaaa taatagattg gctattgaaa atattaaaga tgatattgag
     5461 aatggataca ctgctttcat tatcacgtta aagatgtaaa tagtgttata gaattttatg
     5521 tttcatggat gatttcaacc tttggatttc aggtttttat ggatgaatgc ctgagtccat
     5581 atatacaagg aacagtatga aaaaaataac agggattatt ttattgcttc ttgcagtcat
     5641 tattcttgct gcatgtcagg caaactatat ccgtgatgtt cagggcggga ctgtatcacc
     5701 gtcgtcaact gctgaactga ccggagtaga aacgcagtaa cccgaaatcc tcatttgaca
     5761 agaacaaagc gtgtcaggct gatcctgacg cgcttttttt tgaagtttca caaaaattcc
     5821 atgcgggaga tgagagctaa aatccccgtg cagaactttc caccctgggg gagaaaactt
     5881 gtcattttga cctgttcgcc cttcggaaca gtcgaaatcg atcgcgcatc gctttcgtgc
     5941 atagttatgc aaccctataa aaacgattct gacgcgtttt tctggtttgg cctggtgttt
     6001 tcttgtcttt ttgcgttttt tgcgtcagaa agcgactgag ggcgttttaa tgggtacgta
     6061 caacgggagt tatggtaaat ggatcggttt ttcgggaagg atcgacagga tttgccgttg
     6121 ggtgtagtgt aagcgactga aaaacaaacg cccctgaaat catgtccaga tccggcaaga
     6181 ttcaacatga aatacagagg gcgtgtaggt
//
