LOCUS       NZ_CP118403             4665 bp    DNA     circular CON 29-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2020CK-00223 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP118403
VERSION     NZ_CP118403.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907158
            Assembly: GCF_028872175.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00223
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028872175.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 05:49:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,523
            CDSs (total)                      :: 5,398
            Genes (coding)                    :: 5,226
            CDSs (with protein)               :: 5,226
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 61 of 172
            Pseudo Genes (incomplete)         :: 105 of 172
            Pseudo Genes (internal stop)      :: 24 of 172
            Pseudo Genes (multiple problems)  :: 16 of 172
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118403.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00223"
                     /isolation_source="Tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="2020-06-03"
     gene            400..723
                     /locus_tag="PWK27_RS27560"
                     /old_locus_tag="PWK27_27560"
     CDS             400..723
                     /locus_tag="PWK27_RS27560"
                     /old_locus_tag="PWK27_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            932..>1366
                     /locus_tag="PWK27_RS27565"
                     /old_locus_tag="PWK27_27565"
                     /pseudo=""
     CDS             932..>1366
                     /locus_tag="PWK27_RS27565"
                     /old_locus_tag="PWK27_27565"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1401..1886
                     /locus_tag="PWK27_RS27570"
                     /old_locus_tag="PWK27_27570"
     CDS             1401..1886
                     /locus_tag="PWK27_RS27570"
                     /old_locus_tag="PWK27_27570"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012565228.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1890..2105
                     /locus_tag="PWK27_RS27575"
                     /old_locus_tag="PWK27_27575"
     CDS             1890..2105
                     /locus_tag="PWK27_RS27575"
                     /old_locus_tag="PWK27_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_012565230.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMLRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2451..4007
                     /locus_tag="PWK27_RS27580"
                     /old_locus_tag="PWK27_27580"
     CDS             2451..4007
                     /locus_tag="PWK27_RS27580"
                     /old_locus_tag="PWK27_27580"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589620.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_032993376.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVLV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPLKSVTGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHNLFGDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTYIKVMMERVDFLNEVALKDPLTGVNNRLGFLSKIQNHKRDSGYSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     Typing          114..287
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(3..190)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.435"
                     /coverage="91.0"
                     /evalue="6.33e-53"
     Typing          complement(4303..4665)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.247"
                     /coverage="91.0"
                     /evalue="3.28e-155"
     Typing          401..717
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="1.81e-61"
     Typing          714..2209
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggccttctc gcttccctgt tacgttcctt cctggcatct tccaggaaat ttccgcccca
       61 cctgtaagcc ggcaccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      121 ggaagcggaa tatatcctgt atcacatatt ctgctgacgc gccggtgccg ccttttttct
      181 cctgccacat gaagcacttc actgacaccc tcatccgtgc caacatatta agccagtata
      241 cactccgcta gcgctacgtg actggttcag ggctgcgccc cgaaacccgc caaaagcctg
      301 acgcgcctgc ggcttgtcca accccgtgcc gaccgggaaa ggttttcccg cccgtcccgg
      361 cgttatcagg agaccggttt gtcagagaaa cggaacaaga tgctcaccat gtgggtgacg
      421 gaggacgagc accggcggct gcttgagcgc tgtgacggca ggcagctggc ggcgtggatg
      481 cggcagacct gcctggacga aaagccggca cgttccggaa agctcccctc aatctcgccg
      541 gcgctgctgc gccagcttgc cggcatgggc aataacctga accagatagc ccgccgtgtt
      601 aacgccggcg gcggtaccgg ccatgacagg gtgcagattg ttgcggcgct gatggccatc
      661 gatgccggac ttgagcggct gcggcacgcc gttcttgaga aaggaacgga cgatgatcgt
      721 taaatttcat ccacgcggtc gcggcggtgg cgccgggccg gtggactatc tgctgggtaa
      781 agaccggcag cgtgaaggcg ccaccgtcct gcaggggaag ccggaagaag tccgggagct
      841 gattgacgcg tcgccttacg ttaagaaata cacctccggg gtactgtcgt ttgcggaagc
      901 ggatttacct tccggccagc gggaaaagct gatggcaagc tttgagcggg ttctgatgcc
      961 cggacttgat aaagaccagt acagcatcct gtgggtggag catcgtgaca aggggcggct
     1021 tgagctgaat tttctgatcc cgaacacgga gctgctgacc ggcagacggc tccagccgta
     1081 ttacgatcgc gccgaccgtc cacgcatcga tgcctggcag accatcgtga acggcagact
     1141 ggggctgcac gacccgaacg caccggagaa ccggcgggca ctggtcacac cgtctgcact
     1201 gcctaaagcg aagcaggagg ctgcagaagc gattacacgc ggtctgcttg ctcttgcctc
     1261 atccggggag ctgaaaaccc gtcaggatgt cactgaggcg ctggaaagcg caggttttga
     1321 ggtggtccgc accacaaaaa gcagtatcag cattgccgac ccggacgggg ggcgaaacat
     1381 ccgactgaag ggagccattt atgaacagtc ttttaacgct ggcgaaggac ttagagcaga
     1441 aatcgaaagc gcagcagcag aataccggcg agatgctgaa agccgcattc agcgagcacg
     1501 agaaatctgt aaaagcggaa ctgaacgaaa gcgcgaagag aatcagcgcc gccatcctcg
     1561 accacgaccg gaaactgtcc tcagccatga gccagcgcac gaaaggcatg gtgcgcatgg
     1621 tcagccagac gtggctgacc atcgtcctgg tctccgcgct gctgatcgcc tcgagcgcgg
     1681 gcattctgtg gtggcagggg cagcagatac tcgacaacta cacgaccatc cgggagcaga
     1741 agagcacgca ggccatgctg tcagagagga acagcggcgt gcagctctcg acctgcggcg
     1801 agcagagacg ccgctgcgtg aaggtgaacc cggaagcggg acggttcgga gaggattcga
     1861 gctggatgat actggcgggg aaatagcaca tgacggagct ggaaaaacag ttgctgagcg
     1921 cattagagca gctacagcag gactactcga aaaggctgga cgagtgggag agcgccttcg
     1981 cggaatggcg gacgatgtct ggtcttatgc tacgggagaa cgcagcgctg agcgagcgcg
     2041 tcacgggctt gagcaggcag gtgcagagtt tgagcgagca gctgcgccgg ttgtcgagcg
     2101 actgaatgcc gtcgagctgc accgggagca ggagcgcgtg gtgcagcatc agaaagcgct
     2161 ggagctggag cgatctcagc gacagcggac gtataacagc ccttcactgt gataacctga
     2221 taaaacgggg ctttcggcgg acatctgcgc tgaaagccgg agccattcac ccgactgcat
     2281 agccatgcag ccgggtgaaa tttatctggc gcagaatgcg cggtttagcg gagagtttgc
     2341 tgccggtttt accgggctga cgccgcattt gttccgaacc cgtccggggc ggtgctgata
     2401 agggatgtta tggtaaatgc ggcatttttt attatatgga gtgtattttt atgagaatta
     2461 aaaagcctca ccttcgtccg atagtcataa ccttgtccgt tggtgggatt ttgttaactt
     2521 ctatttttct gattgccgtt attatggttt ttcagcgcga aaatatagaa aaagatttgc
     2581 tttatgctaa ttcatcgtac gctatgaaaa tgtcagatgt aatgagtggt tatattgaaa
     2641 tggctcaagg tgaattagca tatggagcaa aaaaaataaa atcaactaat gatattgact
     2701 cacttaaaga tgaggcggat aggttaagat tgcagtctgg tatgtttaat tcagttcttg
     2761 ttgttagtaa tgatgctgtt gttctggcga cttcgccaga atctcttgat ttagttggta
     2821 cccgcttgaa ttcaagtgcc agcaaattgg cgattgagga aaagaaacca ttcatatctt
     2881 caccgttaaa atcagttact gggaatttga ttattttgtt gtcacatcct atttatgata
     2941 agggtggcaa ttacataggg tatgtcggtg ggtctatata tctaaaaaaa cataatttat
     3001 ttggcgatgt tttgagcagg cattttttca agaacaacac tgaaattagc attgttagtg
     3061 atgatgggaa tgtgatcttt aataacgata aaagtgctgt tgatcggcct atggttatgt
     3121 ctgatgattt gaaatcaaag ttaactgcgt ctgatagagg tgagggggca tttttttctt
     3181 cggggcatag atatcttctt ggttatgcta gtatgaaaaa tactgcttgg aatgtgttgg
     3241 tttatagtcg tgctgacagt gtgactacta ttcttattgc ttatgctaaa agtgtacttg
     3301 ttctgctaat tataataatt attgtgtttt cacttatttc atatttcatt gcctccagta
     3361 tttcacttcc tttagaaaag cttgctatat ctaccaatgc taaagatatc ggagcttcgc
     3421 tagtttatat taaagctatc aatgcttggt atgccgaagc tgataggctt aaaaaggcgc
     3481 tttttacgta tattaaggta atgatggaaa gagttgattt tctaaatgag gtggcgctta
     3541 aagatcccct taccggcgtg aacaatagat tggggttttt aagtaaaata cagaatcata
     3601 agcgggattc agggtattca gttattgtaa ttgatgttga tttttttaaa gagataaatg
     3661 acagtttcgg gcatggggtc ggagatgagg tgcttgtttc tttggctcat attattaatt
     3721 cctgttgccg tagtgaagat gttgtttgtc ggtttggtgg cgaagagttt gttgttttcc
     3781 tgccgaatac atctgtggta acagctaagt gtgttgcgga gcgtattagg agtgttattg
     3841 aaagtactgt cttccctaat aacttgcggg tcaccatatc tgctggtgtg gcaacacaag
     3901 atgatcctct tggtaggatc gattttttgc tgaaaaatgc tgacgatgct ctttatcaag
     3961 ccaaaaggga ggggcgtaat aaagtgattg tatatagcgc tatttgagtt ttttgatgat
     4021 gctttacgcg ctagtgtgtc gttttttttg tggcgaagag tgggcaagaa gcggatcctc
     4081 ttgttatccc ttcgatgtcg tcgtaatctc ataccctgta aacaaaaaaa ccaccgctac
     4141 caacggtggt tcgtttgccg gaattcagag ctaccaactc tttcttccga aggtaactgg
     4201 ctttcacgga gcgcaggtac caaaactgtc cttccagtgt agccgcagtc aggccaccac
     4261 ttcaagaact ctgtaggaca ctctactact ccgctccgtg aatcctctta ctaatggcta
     4321 ccgccactgg cgatttttcg tgtctgttcc gggttggact caagatgata gttaccggga
     4381 caacgtggta gtcgggctga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     4441 ccttcccgga accgagtgtc aggcgtggaa tgagataaac gcggccataa cagcggaatg
     4501 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccacc agggggaaac
     4561 gcctggtatc tttaagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt
     4621 gatgcttgtc aggggggcgg agcctatgga aaaacggctt cgccg
//
