LOCUS       NZ_CP118401            12224 bp    DNA     circular CON 29-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2020CK-00223 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP118401
VERSION     NZ_CP118401.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907158
            Assembly: GCF_028872175.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12224)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00223
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12224)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028872175.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 05:49:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,523
            CDSs (total)                      :: 5,398
            Genes (coding)                    :: 5,226
            CDSs (with protein)               :: 5,226
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 61 of 172
            Pseudo Genes (incomplete)         :: 105 of 172
            Pseudo Genes (internal stop)      :: 24 of 172
            Pseudo Genes (multiple problems)  :: 16 of 172
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118401.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12224
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00223"
                     /isolation_source="Tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2020-06-03"
     gene            join(11203..12224,1..4)
                     /gene="istA"
                     /locus_tag="PWK27_RS27470"
                     /old_locus_tag="PWK27_27470"
     CDS             join(11203..12224,1..4)
                     /gene="istA"
                     /locus_tag="PWK27_RS27470"
                     /old_locus_tag="PWK27_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490293.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="WP_004199214.1"
                     /translation="MLTQEQTVEIRVLARQGHSIRHIARTLGVSRNTVRRYLRDPSVAR
                     YQPREPRPTKLGPFESYLRQRVEQAHPIWLPATVLNREIRAQGYGGGLSLLRAFLATLK
                     PARREAGPAVRFETEPGRQLQADFVVLRRARSPMSAFVATLGYSRMTFVTFVPDESFES
                     VRDSLLLAFDYLGGVPREVLFDNMKTVVLERDAYGDGKHRFHPGLLQLADDLGFRIRLC
                     RPYRAQTKGKVERFNRYFRESFYNPLLTRMKGTGLLLDCAAANRRVRDWLADEANVRVH
                     ATLNERPIDRWRQEREHLQPLPSRVRRDEAPLLDNSLRPVPLESLQHPLSVYDAIGEAC
                     R"
                     /name="istA"
     gene            1..780
                     /gene="istB"
                     /locus_tag="PWK27_RS27475"
                     /old_locus_tag="PWK27_27475"
     CDS             1..780
                     /gene="istB"
                     /locus_tag="PWK27_RS27475"
                     /old_locus_tag="PWK27_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISKpn7 family helper ATPase
                     IstB"
                     /protein_id="WP_004152394.1"
                     /translation="MNLQHERIDALCQQLKLERVATHYPVLAQQAVREDFSFTDFLEHL
                     LRHEAGCRQQRSRELLTRMACFPGIKTLEDYDFSFNPGVPKALVQELGSLAFVERAENV
                     VLIGPSGIGKTHLAIALGYKAAQAGVKTRFITAADLMLQLSTAQRQGRLKEYFNRAILG
                     PRLLVIDEIGYLPFGREEANLFFQVVAKRYEKGAMILTSNLPFSQWADAFAGDTTLTAA
                     MLDRLLHHAHILTLSGESYRLKDKRKAGVVRKNSKPE"
                     /name="istB"
     gene            1167..2048
                     /locus_tag="PWK27_RS27480"
                     /old_locus_tag="PWK27_27480"
     CDS             1167..2048
                     /locus_tag="PWK27_RS27480"
                     /old_locus_tag="PWK27_27480"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="WP_004199234.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-2"
     gene            complement(2298..3617)
                     /locus_tag="PWK27_RS27485"
                     /old_locus_tag="PWK27_27485"
     CDS             complement(2298..3617)
                     /locus_tag="PWK27_RS27485"
                     /old_locus_tag="PWK27_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /protein_id="WP_004152397.1"
                     /translation="MAASYLPYRPDQSYLLPPSLGEWLPEGHLAYFISETVDTLDLSAF
                     HARYAGGGPGNQPFHPAMMVKVLIYGYASGVFSSRKLARKLYEDVALRVLAAGNFPAHR
                     TLSDFRALHLTELENLFVQVVQLARECGLVKLGTIAVDGTKVKANASRHKAMSYKRMKP
                     AEDELHCEIKALLDRAKATDDQERNEPELDIPAEISRREKRLEAIQAAKARLEARQREA
                     DQARGRSEDDGRRPRHPDGSDKGGGSYKREFGVPDDRDQESFTDPDSRIMKHAGGGSEQ
                     SYNGYTAVDAEHQIIVAAELTNCAADSQALLGMLAAVQANTGEMPAQTLADAGFRSEAV
                     LAKVADHHGDVIVALGREGREDAKVNAKTHPHTAAIAAKLKTEQGDAAYRRRKSIVEAP
                     NGWIKAVMGLRQFSMRGLDKVQAEWKLVCMALNLRRMAYL"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            complement(4367..4603)
                     /locus_tag="PWK27_RS27490"
                     /old_locus_tag="PWK27_27490"
     CDS             complement(4367..4603)
                     /locus_tag="PWK27_RS27490"
                     /old_locus_tag="PWK27_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001676031.1"
                     /translation="MNRLTNLTPAEKKFLDDAIAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSEFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            5302..5433
                     /locus_tag="PWK27_RS27660"
     CDS             5302..5433
                     /locus_tag="PWK27_RS27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032159035.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDKAPLAAAIGNWISRAG"
                     /name="hypothetical protein"
     gene            5888..6190
                     /locus_tag="PWK27_RS27495"
                     /old_locus_tag="PWK27_27495"
     CDS             5888..6190
                     /locus_tag="PWK27_RS27495"
                     /old_locus_tag="PWK27_27495"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_274759741.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIVPVLLRVWSTELALTNQRVIAKVGLIRRNTVELRIDKVESLGIHQGILGRIFN"
                     /name="PH domain-containing protein"
     gene            complement(6242..7957)
                     /gene="tnpR"
                     /locus_tag="PWK27_RS27500"
                     /old_locus_tag="PWK27_27500"
     CDS             complement(6242..7957)
                     /gene="tnpR"
                     /locus_tag="PWK27_RS27500"
                     /old_locus_tag="PWK27_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family resolvase TnpR"
                     /protein_id="WP_004152391.1"
                     /translation="MKTGQTTASSSTPVGFPDADSLAALRGWYAGLSSRAAVEHYLAAR
                     KAAGQSARGMLGHIRRQLIVFANSRHRPDLADVFQHAPEERQGRAGAVERAIRLLPTLP
                     APKPQISDSIDQWLSPRTVRALQAYGIHTLSALTVRIPRRRGWWKAIEGLGMTGARQVE
                     AFFAAHPQLTERARALIPIIQPSGIVPWEQLRLPNEVDGSQGNFRAPRNACTLNATNDY
                     EAVQAWLALHETVATQRAYRKEAARLILWAIVERGRALSSLTTDDAIAYRAFVRHPTPR
                     ERWVGPPRPRDSVEWRPFSGGLSARSAAYALTVLSALFRWLIEQRYVLANPFAGVKVRG
                     HALRPALDTARGFTEGEWLLLRAIADGLEWSYGWSEPAAQRLRFMLDFGYATGLRASEL
                     VGAVLGNVRLDEHGDHWLHLVGKGGKPGKVALPPLAYTALDQYLAQRQLPVSRERWNPK
                     TPLVGSLGEDGNAGITGPHLWCVLRRFFTQAAEAIEADHPVAAEKLRRATPHWMRHSHA
                     SHALARGAQLTAVRDNLRHASIATTSMYLHGDELERARQMRQAFGARHVKRGVD"
                     /name="tnpR"
     gene            8067..11096
                     /gene="tnpA"
                     /locus_tag="PWK27_RS27505"
                     /old_locus_tag="PWK27_27505"
     CDS             8067..11096
                     /gene="tnpA"
                     /locus_tag="PWK27_RS27505"
                     /old_locus_tag="PWK27_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152392.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family transposase"
                     /protein_id="WP_004152392.1"
                     /translation="MSKKNKLLSVLSDAEQEALYGLPEFDDAQQLEYLAVTETELALAN
                     SRPSLHAKVYCLLQIGYFKAKHAFFSFDWDEVEDDCAFVLSRYFHGEVFEPKAITKHER
                     YTQREQIAKLFGYWPWSAAFLPQLEQQAARTVRRDVTPGFVAAELIVWLNEHKIIRPGY
                     TTLQELVSETLSAERRRLGNLLEQALDESAKAALSQLLVRDDTLSQLAALKQDAKNFGW
                     RQMVREREKRATLEPLHAIAKTLLPKLDVSQQNLLYYASLANFYTVHDLRNLKADQTQL
                     YLLCYAWVRYRQLSDNLVDAMAYHMKQLEDESSAGAKQSFADEQVRRQQETPRVGRLLS
                     LYVDDSVADPTPFGEVRQRAYKIMPRDTLQTTAQRMSVKPASKLALQWQAVDGLADRMR
                     RHLRPLYVALDLSGSNPDSPWLAALAWAKGVFAKQQRLSQRPLDEWPAATLPKRLRPYL
                     LTFDADGKPTGLHADRYEFWLYRQIRKRLQSGELYLDDSLQHRHFSDELVEMDRKIDVL
                     AKMEIPFLQQPVHAQLDALTAELRTQWLAFNRELKQGKLTHLEYNKDTQKLTWRKPKSE
                     NEKAREKAFYEKLPFCDVADVFRFVNDECKFLSAFTPMQPRYAKKVADADSLMAVIIAQ
                     AMNHGNHVMARTSDIPYHVLDSGYQQYLRQATLHAANDCISNAIATLPIFPYYSFDLET
                     LYGAVDGQKFSVERPTVKARYSRKYFGRGKGVVAYTLLCNHIPLNGYLIGAHDYEAHHV
                     FDIWYRNTSDIMPTAITGDMHSVNKANFAILHWFGRRFEPRFTDLNAQLKELYCADDPA
                     QYQACLIRPVGKIDCDLIHREKPNIDRIVATLGLKEMTQGTLIRKLCTYTTTNPTRRAI
                     FEFDKLIRSIYTLRYLRDPQLERNVHRSQNRLESYHQLRSAIAQVGGKKELTGRTDIEI
                     EISNQCARLIANTIIFYNSAILSRLVTKYEAAGNSKALALITKISPAAWRHILLNGHYT
                     FQSSGKTIDLDAIVAGLELE"
                     /name="tnpA"
     ARG             1168..2045
                     /name="blaKPC-2"
                     /gene="blaKPC-2"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(4768..4941)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          4865..5411
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.364"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 atgaacctcc agcatgagcg gatcgacgcc ctgtgccagc aactcaaact cgaacgggtg
       61 gcaacgcatt accccgtcct ggcgcagcag gcggtgcgcg aggacttcag cttcaccgac
      121 ttcctggaac acctgttgcg gcatgaagcg gggtgtcgcc agcagcgcag ccgcgaactg
      181 ctgacgcgga tggcttgctt cccgggcatc aagaccctgg aggactatga cttcagcttc
      241 aaccccggcg tgcccaaggc actggtgcag gagctgggca gtctggcgtt tgtcgaacgg
      301 gccgagaacg tggtgctgat cggaccctct ggcatcggca agacccacct cgccatcgcc
      361 ttgggttaca aggccgcgca agccggggtc aagacacgct tcatcacggc agccgacctg
      421 atgctgcaac tgagcacggc gcagcgacaa gggcggctga aggaatactt caaccgcgcc
      481 atcctcggtc cacggctgct ggtgatcgat gagatcggct acctgccttt cggacgcgag
      541 gaagcgaacc tgttctttca ggtggtggcg aagcgctatg agaaaggggc gatgatcctg
      601 acctcgaacc tgccgttcag ccagtgggcc gacgcgtttg ccggcgatac caccctgaca
      661 gccgcgatgc tggatcggct gctgcaccat gcccatatcc tgaccctgag cggcgaaagc
      721 taccgcttga aggacaagag gaaggcggga gtggtcagga aaaattccaa acccgaatga
      781 tccaggtggg tcagtattac tttggtgatt caggggtaaa gtgggtcagt tttcagttgg
      841 tgttgacacc ggcgtaccct cggtgctatc ttcgcgcccc aatagtcggg gcttggccag
      901 gacttcctga ggccgtccgt aacgtggatg ccgaggtcag gcgaggtggc cgacccatga
      961 acgccgacct gattcgtttt tcaatagcgc tggacgttgt ggtgccaggg acttaccaac
     1021 ccgatgtgtg cccatccggg gcagttacag ccgttacagc ctctggagag ggagcggctt
     1081 gccgctcggt gataatccca gctgtagcgg cctgattaca tccggccgct acacctagct
     1141 ccaccttcaa acaaggaata tcgttgatgt cactgtatcg ccgtctagtt ctgctgtctt
     1201 gtctctcatg gccgctggct ggcttttctg ccaccgcgct gaccaacctc gtcgcggaac
     1261 cattcgctaa actcgaacag gactttggcg gctccatcgg tgtgtacgcg atggataccg
     1321 gctcaggcgc aactgtaagt taccgcgctg aggagcgctt cccactgtgc agctcattca
     1381 agggctttct tgctgccgct gtgctggctc gcagccagca gcaggccggc ttgctggaca
     1441 cacccatccg ttacggcaaa aatgcgctgg ttccgtggtc acccatctcg gaaaaatatc
     1501 tgacaacagg catgacggtg gcggagctgt ccgcggccgc cgtgcaatac agtgataacg
     1561 ccgccgccaa tttgttgctg aaggagttgg gcggcccggc cgggctgacg gccttcatgc
     1621 gctctatcgg cgataccacg ttccgtctgg accgctggga gctggagctg aactccgcca
     1681 tcccaggcga tgcgcgcgat acctcatcgc cgcgcgccgt gacggaaagc ttacaaaaac
     1741 tgacactggg ctctgcactg gctgcgccgc agcggcagca gtttgttgat tggctaaagg
     1801 gaaacacgac cggcaaccac cgcatccgcg cggcggtgcc ggcagactgg gcagtcggag
     1861 acaaaaccgg aacctgcgga gtgtatggca cggcaaatga ctatgccgtc gtctggccca
     1921 ctgggcgcgc acctattgtg ttggccgtct acacccgggc gcctaacaag gatgacaagc
     1981 acagcgaggc cgtcatcgcc gctgcggcta gactcgcgct cgagggattg ggcgtcaacg
     2041 ggcagtaagg ctctgaaaat catctattgg cccaccaccg ccgcccttgc gggcggcatg
     2101 gattaccaac cactgtcaca tttaggctag gagtctgcgc ggcagagccg tgtgaccggt
     2161 tttctgtaga gcactgacga tggcggcggc gctctctgca attggcaagg cgtcggcgcc
     2221 aaggatacca atcttgcggc gcgcggcgtg ttatgacgac tggggtgcat ttgagccgcc
     2281 ccatttaacc ttcgccctca cagatacgcc attcgcctca gatttagcgc catgcagacg
     2341 agcttccact cggcttgcac cttgtccagg cccctcatgc tgaactgacg caatcccatc
     2401 accgccttga tccaaccatt cggagcctcc acgatcgact tgcgccggcg gtaagctgca
     2461 tcgccttgct ccgttttcaa tttcgccgca atcgccgccg tatgcggatg ggtcttggca
     2521 ttgaccttgg catcttcacg tccctcgcgg ccgagggcaa cgatgacatc gccgtggtga
     2581 tcggcgacct ttgccagaac agcctcacta cggaatcccg catccgccag cgtctgggcc
     2641 ggcatttctc cggtgttggc ctgaactgct gccagcatgc ccagcagcgc ctgactgtcc
     2701 gcggcgcagt tggtcaactc cgccgccacg atgatctggt gctcggcatc gaccgctgtg
     2761 tacccgttgt agctctgctc ggagccacca ccggcgtgtt tcatgatccg gctgtccgga
     2821 tcggtgaagc tttcctgatc acggtcatcc ggcacaccaa actcgcgttt gtacgagcca
     2881 ccgcccttgt ccgagccatc cggatggcga ggccggcggc catcgtcttc gctgcgcccc
     2941 cgggcctggt ccgcttcacg ctggcgcgct tccaggcgcg cctttgccgc ctggatcgcc
     3001 tccaggcgct tctcgcggcg agaaatctcg gcaggaatgt ccagctccgg ctcgttacgc
     3061 tcctggtcgt cggtagcctt ggcgcgatca agcagcgcct tgatctcgca atgcaattcg
     3121 tcctcggccg gcttcatgcg cttatagctc atcgccttgt ggcggctggc gttggctttt
     3181 accttggtgc cgtcgaccgc gatcgtgcca agcttcacca gcccacattc gcgcgccagt
     3241 tgcaccacct gaacgaacaa attctcaagc tcggtcaggt gtagggcacg gaagtcactc
     3301 agcgtgcggt gggccgggaa gtttccagcg gccagcacac gcaacgcgac atcctcgtac
     3361 agcttcctgg ctagtttgcg cgaagagaag acgccgctcg cgtaaccata gatcagcact
     3421 ttgaccatca tcgccggatg aaacggctga ttgcccggac caccgccggc ataccgggcg
     3481 tggaatgcgc tcaagtccag cgtatcgaca gtctcgctga tgaagtaggc aagatgccct
     3541 tcaggtagcc actctcccag agaagggggc agcagatagg attggtcggg tcggtaagga
     3601 aggtaactgg cagccatccc cgtatcgtcc tcgatcttcc gccgatttgg cttctgccgc
     3661 gcaggctcct aggccaccgt agctaaacat acgagaaaaa ccgcttcttg agcgctctct
     3721 gagaccacct tgccgacaac gcagaaacgg ttgcggaaaa atcggcgttt ccggcgtacg
     3781 ccacccgacc tggacgaact attgcggacc tggaactgga gtagcggaat tccccggggt
     3841 ttcagcgtcg cgccacagcg cctcagatag atgcggtagc ctttggcggg tcgtcatgcc
     3901 gcggaccacc cccccaaaaa attccacaat gcggaatggc ggaattttct ggggttcccg
     3961 cttacccccc caattatggg tcgatcgtaa ttaaaggtac cggcggaacc aacacgccaa
     4021 ttccaaacat caaggcacca atgcaattca gatctattgt gaacaatcac attgaggaaa
     4081 tggattctaa gcagcagtag tttcctcata agcattacaa aaaaagcccc gcagggcttt
     4141 ttttgtacct gccagtgagt cagatttacc ataacatccg ttgtctgcac tgcctgagag
     4201 gccctcagcg cgtttctgcg gcctgacagc cgtttccggc acctttcacc gcaccaaccc
     4261 ctgaaaaacg cccctgatga tttctgcgcg tctgcatggc tattcacagg agtgaatggc
     4321 gggcaatttc ggagtgtcag caccactaaa ccgccaaaag gcgcaatcag cggcgcggcg
     4381 cgcgcggacg tgaccaggca aactcggact gctgccgctc atcctcacgt aatgcacgct
     4441 ggcggtcggc gtaacgctgc gactcaatct gcgcacgcgc tctgttcagg acgatatgcc
     4501 ggttcggctg atttagcttt ttgcctgacg ctctctcagc tgcggcaata gcatcatcaa
     4561 gaaatttttt ctctgcaggt gtcaggtttg tcagtctgtt catagcattc tcccatcaga
     4621 ataacgccgc acagtctgcc agacacagca gaaccgatga agcgctggcg gtgctgaaag
     4681 acagccagaa acggtgaacg ggagcggatt ttaatccggt gacgtgagac gttgggcaag
     4741 ccgcaggcgc gtcagtggtt ttagcgggtt tcggggcgca gccctgaacc agtcacgcag
     4801 cgctagcgga gtgtatactg gcttactatg ttggcacgga tgcgaatatc agtgaagtgc
     4861 ttcatgtggc aagagaaaaa aggcggcacc ggcgcgtcag cagaatatgc gatacaggat
     4921 atattccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4981 gcgggcttac tggcggggcg gaaatttcct ggaaaatgcc aggaagaaac ttaacaggga
     5041 agcgagaagg ccacggcgaa gccgtttttc cataggctcc gcccccctga caagcatcac
     5101 gaaatctgac gctcaaatca gtggtggcga aacccgacag gacttaaaga taccaggcgt
     5161 ttccccctgg tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
     5221 ttccgctgtt atggccgcgt ttatctcatt ccacgcctga cactcggttc cgggaaggca
     5281 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgccttt
     5341 tccggtaact atcaacttga gtccaacccg gaaagacacg acaaagcgcc actggcagca
     5401 gccattggta actggattag cagagcggga taatgtaggc agtgctacag agttcttgaa
     5461 gtggtggcct gagtgcggct acactggaag gacagtttag gtatctgcgc tctgctgaag
     5521 ccagttacca cggttagaaa atctgccagg atttactaac cttcgaaaaa ccacctgcca
     5581 gggtggtttt ttcgtttcca aggcaagaga ttacccgcag agaaagacga tctcaagaag
     5641 atcatctgtg atattcgaca gatatgggat aaaggtttct aacgccagga gtatatgggg
     5701 cgtagcagca gcgttcacca ccgttgaatc aagctacttt catggcaaga aataaaagaa
     5761 ccgagcagtc cgagagtcgc tacgctcccc ttaatttttg tatatctacc acgcggatat
     5821 aaggtttcta ttgccgattc atcttaaaaa gggaaaaatc ctattttttc taaatggatg
     5881 taaccttatg ggaagctacg tagaccaatc attgacgaga aatgaatctg taatatctcg
     5941 cgctcaaacc tcctggattc caacaatcat cccagtaatt atcggaatcc tgttgctacc
     6001 tttttacggc ttaggattac ttataatagt ccctgtgctt cttagagttt ggtcaacgga
     6061 gcttgcattg actaaccaaa gagtcatcgc aaaagtaggg ttgatccgac gtaatacggt
     6121 ggaactccgt attgataaag ttgaaagcct tggcatacat caagggatac taggacgaat
     6181 ctttaattag gggttctaag cgggaatccc agaaaattcc gtcattccgt atgatgaaaa
     6241 ttcagtcgac tcctcgcttg acgtgacgag cgccaaaagc ctgccgcatc tgcctggcgc
     6301 gctcgagctc atcgccatgc aaatacatcg acgtcgtggc gatcgacgca tggcgtaggt
     6361 tgtcgcgcac cgcggtgagc tgagctcccc gggccaacgc atggctggca tgactgtgac
     6421 gcatccaatg aggcgtggca cggcgcagct tttcggcggc gaccggatga tcggcctcaa
     6481 tggcctcagc ggcttgcgta aagaatcttc gcaacacgca ccaaaggtga gggccggtga
     6541 tgccggcatt accgtcttcc ccaagactgc ccaccagggg cgttttcgga ttccaccgtt
     6601 cccggctgac tggcagttga cgctgtgcca agtattggtc cagcgccgta tacgcgagcg
     6661 gcggcaaggc caccttacct ggtttgccac ctttgccaac caaatgcagc cagtggtcac
     6721 cgtgctcgtc gaggcggaca ttacccagga ctgcgccgac cagttcgcta gcgcgcaggc
     6781 ccgtggcata gccgaagtcg agcatgaagc gcaggcgttg tgctgccggc tcggaccagc
     6841 cgtaggacca ttccaggccg tctgcaatgg cgcgcagcag tagccattct ccctcggtaa
     6901 agcctcgcgc cgtatccagt gcgggccgca aggcgtgacc gcgtaccttg acgccggcga
     6961 aagggttggc cagcacatag cgctgctcga tgagccaccg gaacagcgcc gagagcaccg
     7021 tcagcgcata cgctgccgag cgtgcggaca agccgcccga gaaggggcgc cattccaccg
     7081 aatcgcgtgg tcgaggtggc cccacccaac gctcgcgcgg ggtcgggtgc cggacaaagg
     7141 cccggtacgc gattgcatcg tccgtggtca gggatgatag cgcccgtccg cgttcgacga
     7201 tggcccacag gattagccgc gcagcctcct tgcgataggc gcgctgtgtg gcgacagtct
     7261 cgtgcagcgc cagccaggcc tgcacggcct cgtagtcgtt ggtcgcattc agcgtgcaag
     7321 cgttgcgcgg ggcccggaaa ttgccctgcg agccgtcgac ttcgttcggc agacgcaact
     7381 gctcccacgg cacgatgccg ctgggctgaa taatcgggat cagggcccgg gcgcgctcgg
     7441 tgagctgcgg atgtgcggca aagaaagcct cgacctgccg ggcgcctgtc atgccgagtc
     7501 cctcgatggc tttccaccag ccacgccggc ggggaatgcg cacggtcagc gccgagaggg
     7561 tgtggatacc gtacgcctgc agtgccctga cggtcctcgg agacagccac tggtcgatgc
     7621 tgtcgctgat ctggggcttc ggcgccggca gcgtgggcaa gagccggatc gcgcgttcga
     7681 cggcacccgc gcgcccctgt cgctcttcgg gggcgtgctg gaacacgtcg gccagatccg
     7741 gccggtggcg gctgttggcg aaaacgatga gctggcggcg gatatgcccc agcatccctc
     7801 gcgctgattg accggcggcc ttgcgtgccg ccaggtagtg ttcaacggcc gcgcgagagc
     7861 tgagtcccgc gtaccagccg cgcaaggcag ccagcgaatc cgcatcggga aagccgactg
     7921 gggtagaact cgaagccgta gtctggcccg ttttcatatg gacaggttgc gattcgaaaa
     7981 gcgagtaacg cgataataac gattatttca gtagaatcga aaatgcccca tgtttctacg
     8041 aaacctcaag atagcaagaa cgggccatga gcaagaagaa caagctactc agcgtcctat
     8101 ccgacgccga acaggaggcc ttgtatggcc tgccggagtt cgatgatgcg cagcagctgg
     8161 aatatctggc tgtgacagaa accgagttgg cgcttgccaa cagccggccc agtcttcatg
     8221 ccaaggtcta ttgcctcttg cagatcggct acttcaaggc caagcatgcc ttcttcagtt
     8281 tcgactggga cgaagtcgag gacgattgcg ctttcgtgct gagccgctat ttccacggcg
     8341 aggtgttcga acccaaggcg atcaccaagc atgaacgcta cacccagcgc gagcagatcg
     8401 ccaaactatt cggctactgg ccgtggtcgg ccgccttcct gccgcagctc gagcagcagg
     8461 ccgcacggac cgtgcggcgt gacgtaacgc cggggttcgt cgccgccgag ctgatcgtct
     8521 ggctcaatga gcacaagatt atccggcccg gctatacgac cttgcaagag ttggtcagcg
     8581 aaacgctatc cgccgagcgc cggcgcctgg gcaatctgct ggagcaagcg ttggacgaat
     8641 ctgccaaggc cgcgctgagc cagcttctgg tccgtgatga caccctgtcg caactggcgg
     8701 cgctcaagca ggacgccaag aatttcggct ggcgccagat ggtccgcgag cgcgaaaagc
     8761 gcgccacgct ggagccgctg cacgcaatcg ccaagacact gctgcctaaa ctcgacgtct
     8821 cgcagcagaa cctgctgtac tacgcgagcc tggcaaactt ctacaccgtc cacgacctgc
     8881 gcaatctgaa ggccgatcag acccaactct acctgctgtg ctatgcctgg gtacgctacc
     8941 ggcagctctc cgacaacctg gtcgatgcga tggcgtacca catgaagcag ttggaggacg
     9001 aaagcagcgc tggtgccaaa caatcctttg ccgacgagca ggtgcgccgt cagcaggaaa
     9061 cgccgcgggt aggccgcctg ctgtcactct acgttgatga tagcgtggcc gatcccacgc
     9121 ccttcggcga ggtgcggcag cgcgcctaca agatcatgcc tcgcgatacg ctgcaaacca
     9181 cggcgcagcg tatgagcgtg aagccggcga gcaagctggc cttgcagtgg caggcggtgg
     9241 acgggctggc cgaccgcatg cgccgccatc ttcgtccact gtacgttgcg ctcgacctct
     9301 ccggctccaa tccagacagt ccatggcttg ccgcgctggc ctgggccaaa ggtgtttttg
     9361 ccaagcagca gcggctgtcg caacggccac tcgacgagtg gcccgcggcg acacttccga
     9421 aacgcttgcg gccgtacctg ctgaccttcg acgccgacgg taaaccaacc ggcctgcacg
     9481 ctgaccgcta cgagttctgg ctgtaccgcc agatcaggaa gcgtctccaa tcgggtgaac
     9541 tctacctcga cgacagcctg caacaccggc acttctcaga tgaactggtt gagatggaca
     9601 ggaagatcga tgtgctcgca aaaatggaga tccccttcct gcagcaaccc gtccatgccc
     9661 aactcgatgc gctgactgcc gaactgcgca cgcagtggct ggcgttcaac cgcgagctga
     9721 aacagggcaa gctgacgcac ctcgaataca acaaggacac gcagaaactg acctggcgca
     9781 agcccaagag cgagaatgag aaagcgcgcg aaaaggcatt ctacgagaag cttccgttct
     9841 gcgacgtggc cgacgtgttt cgcttcgtca acgacgagtg caagtttctg tcggcgttca
     9901 cgcccatgca gccgcgctac gcgaagaagg tcgccgacgc tgacagcctg atggcggtca
     9961 tcatcgcgca agcgatgaac cacggcaacc acgtcatggc gcgcaccagc gacatcccgt
    10021 accacgtgct ggatagcggc taccagcagt acctgcgcca ggcaacgctg cacgcggcca
    10081 acgactgcat cagcaacgcc atcgccacgc taccgatctt cccatactac tcgttcgacc
    10141 tcgaaacgct gtacggtgcc gtcgacgggc agaaattcag cgtcgaacgg ccgaccgtga
    10201 aggcgcgcta ttcgcgcaag tacttcgggc gcggcaaggg cgtggtcgcc tacacgctgc
    10261 tgtgcaacca catcccgctc aacggctacc tgatcggcgc gcacgactac gaggcccacc
    10321 acgtgttcga catctggtat cgcaacacgt cggacatcat gccgaccgcg atcaccggcg
    10381 acatgcacag cgtcaacaag gccaacttcg ccatcctgca ttggtttggc cggcggttcg
    10441 agccgcgctt caccgacctc aacgcgcaat tgaaagaact ctactgcgct gacgatccag
    10501 cacagtatca ggcgtgcctg attcggccag tcgggaaaat cgactgcgat ctcatccatc
    10561 gcgagaagcc gaacatcgac cggatcgtcg ccacactcgg gctgaaggaa atgacgcagg
    10621 gcacgctgat ccgcaagctg tgcacctaca ccacgacgaa cccgacgcgg cgcgcgatct
    10681 tcgagttcga caagctcatc cgcagcatct acacgcttcg ctacctgcgc gatccgcaac
    10741 tggaacgcaa cgtgcaccgc tcgcaaaatc gcctcgagtc ctaccatcag ctacgctcgg
    10801 ccattgccca ggtcggcggt aagaaggaac tgaccggtcg caccgacatt gaaatcgaga
    10861 tcagcaacca gtgcgcacgg ctgatcgcca acacgatcat tttctacaac tcggcaatct
    10921 tgtcgcggct ggtgaccaag tacgaggcgg ccggcaacag caaggcgctg gcactcatca
    10981 caaaaatttc gcctgcggca tggcggcaca tcctgctcaa cgggcactac acctttcaaa
    11041 gcagcggcaa gacgatcgat ctcgatgcga tcgtcgcagg cctggaactg gagtgacgga
    11101 attttcagcg gttccgtgtt agcagcagtg taaaactgac ccaccccagc gaagtaaaac
    11161 tgatccacct gggctagttt ggcggccttt tcgaggccgt cgatgttgac ccaggagcaa
    11221 accgtggaaa tcagagttct tgcccgacag ggccactcca tccggcacat cgcccgcacg
    11281 ttgggggtct cgcgcaatac cgtgcgccgc tacctccgcg atccgtcggt agcgcgatac
    11341 cagccccgtg aaccacgccc caccaaactg gggccatttg aatcttatct gcggcaacgg
    11401 gtcgagcagg cccaccccat ctggctgcct gccaccgtcc tgaatcggga aatccgcgct
    11461 caagggtatg gcggcggtct ctcgttattg cgcgccttcc tcgccacgct caagcccgcc
    11521 cgccgcgaag caggtccggc cgtccgtttc gagaccgagc ccggacggca gttgcaggct
    11581 gatttcgtgg tcttgcgccg ggccagatcc cccatgtcgg ccttcgtcgc cacgctggga
    11641 tacagccgca tgacgtttgt caccttcgta ccggacgaat ccttcgagtc cgtgcgggac
    11701 tccctgttgc tggcttttga ctatctgggc ggtgtgcccc gcgaggtgtt gttcgacaac
    11761 atgaagaccg tggtgctgga acgcgatgcc tacggtgacg gcaagcaccg ttttcacccc
    11821 ggcttgcttc aactggccga tgacctgggg ttccggatcc ggctgtgtcg cccgtatcgg
    11881 gcccagacca aaggcaaggt cgagcgcttc aatcgctact tccgcgagag cttctacaac
    11941 ccgttgctga cccggatgaa gggcacgggg ctgctgctcg attgtgctgc tgccaaccga
    12001 cgcgtccgtg actggttggc agacgaggcg aatgtgcggg tccacgccac cctgaacgag
    12061 cgccccatcg accgttggcg gcaggagcgg gaacacctgc aacccttgcc atcccgtgtg
    12121 cggcgcgacg aggcccccct gctggataac agtctccgcc cggtaccact ggaatctctc
    12181 cagcacccac tgtctgtcta tgacgcaatt ggggaggcct gccg
//
