LOCUS       NZ_CP118389             4047 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00224 plasmid unnamed6,
            complete sequence.
ACCESSION   NZ_CP118389
VERSION     NZ_CP118389.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907159
            Assembly: GCF_028872235.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00224
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease Submission Group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028872235.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 07:03:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,023
            CDSs (total)                      :: 4,908
            Genes (coding)                    :: 4,818
            CDSs (with protein)               :: 4,818
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 37 of 90
            Pseudo Genes (incomplete)         :: 53 of 90
            Pseudo Genes (internal stop)      :: 18 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            Pseudo Genes (short protein)      :: 1 of 90
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118389.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00224"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA"
                     /collection_date="2020-06-23"
     gene            complement(201..1619)
                     /locus_tag="PWH45_RS25075"
                     /old_locus_tag="PWH45_25075"
     CDS             complement(201..1619)
                     /locus_tag="PWH45_RS25075"
                     /old_locus_tag="PWH45_25075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137464447.1"
                     /translation="MYHKIIGGKKSKGQRNIKKSIEYLLREKNPEQQKDIKILSTTTKQ
                     DLINFENYILDKNKSNPYVCGVLSFEEKSISEELKTKLITEFEDILFAGIEQENRPPVM
                     WVEHNDKGRVELNYLTFNSLQDRRSYTVYLDKRDRGLVNNYSEIINYENNLSSPFEEKE
                     NKNTLTNKPKTNIPEDKKQFIENINTEIMALIINEELENREQTIKYIENMKGVKINRIR
                     KNQISIKSDLFDDDKPIVLKGDIYEEGRDYSDYRKEFNPKPTRDPEFVREKLTELKRTF
                     AERIEKREIRNRSKFKKPSFKNIPENRQSVQREDQDIGYKYTNGDFINVSNNDGFSGMY
                     IRTDNMEIVNNESINTEYKTKTATEETAGTTRTRDIEKINQQQQFVNSRLEEAGRKQQQ
                     FRKSIDTTEGRFGVCRRSVHSIISTFGRYIRTFSAYTRKNEHRQKFEKRRKIEEEKLKI
                     KNQGSYNRRRYKPY"
                     /name="hypothetical protein"
     gene            complement(1621..1968)
                     /gene="mobC"
                     /locus_tag="PWH45_RS25080"
                     /old_locus_tag="PWH45_25080"
     CDS             complement(1621..1968)
                     /gene="mobC"
                     /locus_tag="PWH45_RS25080"
                     /old_locus_tag="PWH45_25080"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_048295903.1"
                     /translation="MSYKDRYNRKHSITLCFNDEEFAQVEELASMLKKPRATAVRDLIL
                     NKDFKLKKEIAKNKTGDVYFELHKIGVNINQIARAINTDIDKFISHEFSEDFLNLFEEI
                     TNEIEEIKRRI"
                     /name="mobC"
     gene            complement(2221..3633)
                     /locus_tag="PWH45_RS25085"
                     /old_locus_tag="PWH45_25085"
     CDS             complement(2221..3633)
                     /locus_tag="PWH45_RS25085"
                     /old_locus_tag="PWH45_25085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_113519630.1"
                     /translation="MIRLKKVFNLTEEEIVLTNEIAEAISKEPTVETHEVNINFIDVLP
                     IIPNPHNLGLSKDIDPLSLIYSKFITQTDIPPALPVFSFLSYLSAFSVNNNIMYKHPTS
                     PADYLNTWTLILAPSGAAKTTAAKIISSSIPKDIEENPMIKPNFEGADGSAAFISELAK
                     AEKKIDNFGKPIQPIFWIEDEYSQFMKKLMPGGSMVETRKTMLKIHDNDKARRVTKNDT
                     IETESIVMSGLFLNTIDSFARNFDQESINDGLGRRHNFVYAERGEKVVPTWTVDQIIES
                     LKEGLDDFFTTVKTNVIYTYSPECRKIYDHFYMVYKEKFDHILGEETNGTFFRTYFMLS
                     WKYAAIYHILLKQEGTEIQAKSFDYGIKVSLMFLSSIKRFLDYKVDLKSGAKQVRKEYL
                     QKAAEKELNNLQKYKEFLLDNIEISLRDFNRKFNIKKIESLKILKTIRENKLIKYHPLF
                     EVLAKEEAKAKK"
                     /name="hypothetical protein"
ORIGIN
        1 ttttaaggtg tcggatcttt tttggaaaat atctgacaag ttttcttggt tttttctgac
       61 actctgacag atttctgaca gaaaaccgtc ctaccggaca ggtcaacccc tcacaagcaa
      121 gaaccagcct gacggctggc tctcacttgt tcacggttaa agttttatca tagtgatcta
      181 gtagtgttgt catcatattt ttaatatggt ttatatcgtc ttctgttgta acttccctga
      241 tttttgattt tgagtttttc ttcttcgatt tttcgtcttt tttcgaattt ttgtctgtgt
      301 tcattcttcc ttgtatatgc tgaaaacgtt cgtatgtatc ttccaaaagt agaaataatt
      361 gagtgtacag atcttctaca tactccaaat cttccctcag ttgtatcaat cgactttctg
      421 aattgctgtt gttttcttcc agcttcttca agtctcgaat taacaaattg ttgttgctga
      481 ttaatttttt caatatctct tgttcttgta gttcctgctg tttcttctgt tgctgtttta
      541 gttttatatt ctgtattaat tgactcatta tttacaatct ccatattatc agtgcgaata
      601 tacatacctg aaaaaccatc gttattacta acattgataa aatcgccgtt agtatattta
      661 tatccaatat cttgatcttc tctttgaaca ctttgtctat tttctggtat atttttgaac
      721 gatggttttt taaactttga tctatttctg atttctcgtt tttcaattct ttctgcaaat
      781 gttcttttaa gctctgtgag tttttctcta acaaactctg gatctcttgt aggttttggg
      841 ttgaattctt ttctataatc gctgtaatct cttccctctt catatatatc tcctttcagg
      901 acaatgggtt tatcgtcatc aaacaaatca gatttaattg atatttgatt ttttcgtatt
      961 ctgtttattt taacaccttt catattttca atatatttga tggtttgttc tctattttcc
     1021 aactcttcat taataattaa tgccattatt tcagtattaa tattttcaat aaattgtttt
     1081 ttatcttctg gaatattggt ttttggtttg ttggttaatg tgtttttatt ttctttctct
     1141 tcgaatggac ttgataaatt attttcataa ttaattattt cagagtagtt atttactaat
     1201 ccacgatctc ttttatctaa atatacagtg tatgatcttc tgtcttgtaa actgttaaat
     1261 gttaaataat ttaactctac cctgcccttg tcgttgtgtt ctacccacat aactggaggt
     1321 ctgttttctt gttcaatccc tgcaaataaa atatcttcaa actctgtaat taatttagtt
     1381 tttaattctt ctgatatgga tttttcttca aatgataaaa caccacacac atatgggttt
     1441 gatttgtttt tatctaaaat ataattttca aaattgatta ggtcttgttt tgtggttgtt
     1501 gataatattt ttatgtcttt ctgttgttct gggtttttct ctctcaataa atattcaata
     1561 ctttttttta tatttctttg acctttactt tttttacctc ctattatttt atggtacata
     1621 ttatattctc ctttttattt cttctatctc atttgttatt tcctcaaaca aatttaaaaa
     1681 atcttctgaa aactcatggg aaataaattt atcaatatca gtatttattg ctcttgcaat
     1741 ctggttaatg tttactccta ttttatggag ttcaaaatat acatcaccag ttttattttt
     1801 tgctatttct tttttgagtt taaaatcttt attgagaata agatctctaa cggctgttgc
     1861 tctgggtttt ttcaacatag aagccagttc ttcaacctgt gcaaattctt catcattgaa
     1921 gcacagggtt atgctgtgct tcctgttgta tctatcctta tagctcatcc tatgcctcct
     1981 gtgttgggtt ttaaggggat tgaaggggga acccctcaca agcgacaata cgaagtattg
     2041 tctgagcttg ctgttatata gttttacact tatatattaa ttaatacata taaaaattaa
     2101 gtcaatacct gaataaaaaa taatcaaatc aaatttgggt tttatatata accgtgaaca
     2161 aataaagcca tttgtaaaat ggcttttttg tgaggggttg acctgtccgg taggacggtt
     2221 ttattttttt gctttggctt cttcttttgc taacacttca aacaatggat gatattttat
     2281 taatttattt tctctgattg ttttaagtat ttttagactt tcgatctttt taatattaaa
     2341 ttttctattg aagtctctta atgaaatttc aatattatca agcaaaaact ctttgtattt
     2401 ttgtaaatta tttaattctt tttctgctgc tttttgtaaa tattctttcc ttacttgttt
     2461 agcaccagat tttaaatcta ctttataatc taaaaacctt tttatactgg ataaaaacat
     2521 taatgaaact ttaatcccat aatcaaaaga ttttgcctga atctcggttc cttcttgttt
     2581 taataaaata tgatatattg ctgcatattt ccaactcaac ataaaataag ttctgaaaaa
     2641 tgttccgttt gtttcttctc ctaatatatg atcaaatttt tccttataaa ccatataaaa
     2701 atgatcataa atctttctac attctgggga atatgtatag attacgtttg ttttaacagt
     2761 agtgaagaaa tcatctaatc cctctttgag agattcgatg atctgatcta ctgtccaagt
     2821 tgggacaact ttttcccctc tttcagcata aacaaaatta tgtcttcttc ctaacccatc
     2881 attaatactt tcctgatcaa agtttctcgc aaaactatca atagtattca aaaataaacc
     2941 actcataacg atactttcag tttcaatagt atcgtttttc gtaactctac gggctttatc
     3001 attatcatgg atttttaaca tcgtttttct ggtttcaacc atagaacctc ccggcattaa
     3061 ttttttcata aactgggagt attcgtcttc tatccagaaa atcggctgta ttggttttcc
     3121 gaaattatca attttctttt ctgcttttgc caactctgaa atgaatgctg ccgaaccgtc
     3181 agcaccttca aaattgggtt taatcattgg gttttcttct atatcttttg gtatagagct
     3241 tgaaataatt ttggctgctg tggtttttgc tgcacctgac ggtgcaagta ttaaagtcca
     3301 agtattcaag taatccgctg ggcttgtagg gtgtttatac attatattat tattaacact
     3361 aaaagcactt aaataactta aaaaagagaa tacaggtaaa gcaggtggta tatctgtttg
     3421 agtaataaac ttagaatata ttaaagataa tggatctatg tcttttgata aacctaaatt
     3481 atgggggttt ggtattatag gtaaaacgtc aataaaattt atattcactt cgtgggtttc
     3541 tactgttggc tctttgctaa ttgcttctgc tatttcgttt gttaaaacta tttcttcttc
     3601 tgttaaatta aaaacttttt ttaatcttat cataataaaa tctcctatta tatctattat
     3661 ataattagtt ataacataag gttttaactt gtcaataata tttttacaaa aaaatatgac
     3721 acattccaaa atgtcttatt ttttggttgg tttttgcgaa gccaaaaaca taccaccccc
     3781 cccctgtttg acactatgac attatgacaa atcacgtaac atactgattt taaagggtta
     3841 aaaaaaattt ttgtcataga tttgtcataa aaaatctaac aacctttgac aaaagagaag
     3901 acatttttcg taatgtattg atgtataaag aaaaaataat ctttctcggt gtcattagtt
     3961 ttatgacaaa atcaaaaatt aaaaaacagg caattttata acctgttttg tcataaaata
     4021 tcccgttttt atttttgact caatgga
//
