LOCUS       NZ_CP118388             4665 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00224 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP118388
VERSION     NZ_CP118388.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907159
            Assembly: GCF_028872235.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00224
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease Submission Group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028872235.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 07:03:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,023
            CDSs (total)                      :: 4,908
            Genes (coding)                    :: 4,818
            CDSs (with protein)               :: 4,818
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 37 of 90
            Pseudo Genes (incomplete)         :: 53 of 90
            Pseudo Genes (internal stop)      :: 18 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            Pseudo Genes (short protein)      :: 1 of 90
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118388.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00224"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="2020-06-23"
     gene            97..420
                     /locus_tag="PWH45_RS25050"
                     /old_locus_tag="PWH45_25050"
     CDS             97..420
                     /locus_tag="PWH45_RS25050"
                     /old_locus_tag="PWH45_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            629..>1063
                     /locus_tag="PWH45_RS25055"
                     /old_locus_tag="PWH45_25055"
                     /pseudo=""
     CDS             629..>1063
                     /locus_tag="PWH45_RS25055"
                     /old_locus_tag="PWH45_25055"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1098..1583
                     /locus_tag="PWH45_RS25060"
                     /old_locus_tag="PWH45_25060"
     CDS             1098..1583
                     /locus_tag="PWH45_RS25060"
                     /old_locus_tag="PWH45_25060"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012565228.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1587..1802
                     /locus_tag="PWH45_RS25065"
                     /old_locus_tag="PWH45_25065"
     CDS             1587..1802
                     /locus_tag="PWH45_RS25065"
                     /old_locus_tag="PWH45_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_012565230.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMLRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2148..3704
                     /locus_tag="PWH45_RS25070"
                     /old_locus_tag="PWH45_25070"
     CDS             2148..3704
                     /locus_tag="PWH45_RS25070"
                     /old_locus_tag="PWH45_25070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589620.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_032993376.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVLV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPLKSVTGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHNLFGDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTYIKVMMERVDFLNEVALKDPLTGVNNRLGFLSKIQNHKRDSGYSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     Typing          4476..4649
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(4000..4552)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.741"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          98..414
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="1.81e-61"
     Typing          411..1906
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcctgcggc ttgtccaacc ccgtgccgac cgggaaaggt tttcccgccc gtcccggcgt
       61 tatcaggaga ccggtttgtc agagaaacgg aacaagatgc tcaccatgtg ggtgacggag
      121 gacgagcacc ggcggctgct tgagcgctgt gacggcaggc agctggcggc gtggatgcgg
      181 cagacctgcc tggacgaaaa gccggcacgt tccggaaagc tcccctcaat ctcgccggcg
      241 ctgctgcgcc agcttgccgg catgggcaat aacctgaacc agatagcccg ccgtgttaac
      301 gccggcggcg gtaccggcca tgacagggtg cagattgttg cggcgctgat ggccatcgat
      361 gccggacttg agcggctgcg gcacgccgtt cttgagaaag gaacggacga tgatcgttaa
      421 atttcatcca cgcggtcgcg gcggtggcgc cgggccggtg gactatctgc tgggtaaaga
      481 ccggcagcgt gaaggcgcca ccgtcctgca ggggaagccg gaagaagtcc gggagctgat
      541 tgacgcgtcg ccttacgtta agaaatacac ctccggggta ctgtcgtttg cggaagcgga
      601 tttaccttcc ggccagcggg aaaagctgat ggcaagcttt gagcgggttc tgatgcccgg
      661 acttgataaa gaccagtaca gcatcctgtg ggtggagcat cgtgacaagg ggcggcttga
      721 gctgaatttt ctgatcccga acacggagct gctgaccggc agacggctcc agccgtatta
      781 cgatcgcgcc gaccgtccac gcatcgatgc ctggcagacc atcgtgaacg gcagactggg
      841 gctgcacgac ccgaacgcac cggagaaccg gcgggcactg gtcacaccgt ctgcactgcc
      901 taaagcgaag caggaggctg cagaagcgat tacacgcggt ctgcttgctc ttgcctcatc
      961 cggggagctg aaaacccgtc aggatgtcac tgaggcgctg gaaagcgcag gttttgaggt
     1021 ggtccgcacc acaaaaagca gtatcagcat tgccgacccg gacggggggc gaaacatccg
     1081 actgaaggga gccatttatg aacagtcttt taacgctggc gaaggactta gagcagaaat
     1141 cgaaagcgca gcagcagaat accggcgaga tgctgaaagc cgcattcagc gagcacgaga
     1201 aatctgtaaa agcggaactg aacgaaagcg cgaagagaat cagcgccgcc atcctcgacc
     1261 acgaccggaa actgtcctca gccatgagcc agcgcacgaa aggcatggtg cgcatggtca
     1321 gccagacgtg gctgaccatc gtcctggtct ccgcgctgct gatcgcctcg agcgcgggca
     1381 ttctgtggtg gcaggggcag cagatactcg acaactacac gaccatccgg gagcagaaga
     1441 gcacgcaggc catgctgtca gagaggaaca gcggcgtgca gctctcgacc tgcggcgagc
     1501 agagacgccg ctgcgtgaag gtgaacccgg aagcgggacg gttcggagag gattcgagct
     1561 ggatgatact ggcggggaaa tagcacatga cggagctgga aaaacagttg ctgagcgcat
     1621 tagagcagct acagcaggac tactcgaaaa ggctggacga gtgggagagc gccttcgcgg
     1681 aatggcggac gatgtctggt cttatgctac gggagaacgc agcgctgagc gagcgcgtca
     1741 cgggcttgag caggcaggtg cagagtttga gcgagcagct gcgccggttg tcgagcgact
     1801 gaatgccgtc gagctgcacc gggagcagga gcgcgtggtg cagcatcaga aagcgctgga
     1861 gctggagcga tctcagcgac agcggacgta taacagccct tcactgtgat aacctgataa
     1921 aacggggctt tcggcggaca tctgcgctga aagccggagc cattcacccg actgcatagc
     1981 catgcagccg ggtgaaattt atctggcgca gaatgcgcgg tttagcggag agtttgctgc
     2041 cggttttacc gggctgacgc cgcatttgtt ccgaacccgt ccggggcggt gctgataagg
     2101 gatgttatgg taaatgcggc attttttatt atatggagtg tatttttatg agaattaaaa
     2161 agcctcacct tcgtccgata gtcataacct tgtccgttgg tgggattttg ttaacttcta
     2221 tttttctgat tgccgttatt atggtttttc agcgcgaaaa tatagaaaaa gatttgcttt
     2281 atgctaattc atcgtacgct atgaaaatgt cagatgtaat gagtggttat attgaaatgg
     2341 ctcaaggtga attagcatat ggagcaaaaa aaataaaatc aactaatgat attgactcac
     2401 ttaaagatga ggcggatagg ttaagattgc agtctggtat gtttaattca gttcttgttg
     2461 ttagtaatga tgctgttgtt ctggcgactt cgccagaatc tcttgattta gttggtaccc
     2521 gcttgaattc aagtgccagc aaattggcga ttgaggaaaa gaaaccattc atatcttcac
     2581 cgttaaaatc agttactggg aatttgatta ttttgttgtc acatcctatt tatgataagg
     2641 gtggcaatta catagggtat gtcggtgggt ctatatatct aaaaaaacat aatttatttg
     2701 gcgatgtttt gagcaggcat tttttcaaga acaacactga aattagcatt gttagtgatg
     2761 atgggaatgt gatctttaat aacgataaaa gtgctgttga tcggcctatg gttatgtctg
     2821 atgatttgaa atcaaagtta actgcgtctg atagaggtga gggggcattt ttttcttcgg
     2881 ggcatagata tcttcttggt tatgctagta tgaaaaatac tgcttggaat gtgttggttt
     2941 atagtcgtgc tgacagtgtg actactattc ttattgctta tgctaaaagt gtacttgttc
     3001 tgctaattat aataattatt gtgttttcac ttatttcata tttcattgcc tccagtattt
     3061 cacttccttt agaaaagctt gctatatcta ccaatgctaa agatatcgga gcttcgctag
     3121 tttatattaa agctatcaat gcttggtatg ccgaagctga taggcttaaa aaggcgcttt
     3181 ttacgtatat taaggtaatg atggaaagag ttgattttct aaatgaggtg gcgcttaaag
     3241 atccccttac cggcgtgaac aatagattgg ggtttttaag taaaatacag aatcataagc
     3301 gggattcagg gtattcagtt attgtaattg atgttgattt ttttaaagag ataaatgaca
     3361 gtttcgggca tggggtcgga gatgaggtgc ttgtttcttt ggctcatatt attaattcct
     3421 gttgccgtag tgaagatgtt gtttgtcggt ttggtggcga agagtttgtt gttttcctgc
     3481 cgaatacatc tgtggtaaca gctaagtgtg ttgcggagcg tattaggagt gttattgaaa
     3541 gtactgtctt ccctaataac ttgcgggtca ccatatctgc tggtgtggca acacaagatg
     3601 atcctcttgg taggatcgat tttttgctga aaaatgctga cgatgctctt tatcaagcca
     3661 aaagggaggg gcgtaataaa gtgattgtat atagcgctat ttgagttttt tgatgatgct
     3721 ttacgcgcta gtgtgtcgtt ttttttgtgg cgaagagtgg gcaagaagcg gatcctcttg
     3781 ttatcccttc gatgtcgtcg taatctcata ccctgtaaac aaaaaaacca ccgctaccaa
     3841 cggtggttcg tttgccggaa ttcagagcta ccaactcttt cttccgaagg taactggctt
     3901 tcacggagcg caggtaccaa aactgtcctt ccagtgtagc cgcagtcagg ccaccacttc
     3961 aagaactctg tagaacactc tactactccg ctccgtgaat cctcttacta atggctaccg
     4021 ccactggcga tttttcgtgt ctgttccggg ttggactcaa gatgatagtt accgggacaa
     4081 cgtggtagtc gggctgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
     4141 tcccggaacc gagtgtcagg cgtggaatga gataaacgcg gccataacag cggaatgaca
     4201 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccaccagg gggaaacgcc
     4261 tggtatcttt aagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat
     4321 gcttgtcagg ggggcggagc ctatggaaaa acggcttcgc cgcggccttc tcgcttccct
     4381 gttacgttcc ttcctggcat cttccaggaa atttccgccc cacctgtaag ccggcaccgc
     4441 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aatatatcct
     4501 gtatcacata ttctgctgac gcgccggtgc cgcctttttt ctcctgccac atgaagcact
     4561 tcactgacac cctcatccgt gccaacatat taagccagta tacactccgc tagcgctacg
     4621 tgactggttc agggctgcgc cccgaaaccc gccaaaagcc tgacg
//
