LOCUS       NZ_CP118387            12020 bp    DNA     linear   CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00224 plasmid unnamed4.
ACCESSION   NZ_CP118387
VERSION     NZ_CP118387.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907159
            Assembly: GCF_028872235.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 12020)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00224
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12020)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease Submission Group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028872235.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 07:03:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,023
            CDSs (total)                      :: 4,908
            Genes (coding)                    :: 4,818
            CDSs (with protein)               :: 4,818
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 37 of 90
            Pseudo Genes (incomplete)         :: 53 of 90
            Pseudo Genes (internal stop)      :: 18 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            Pseudo Genes (short protein)      :: 1 of 90
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118387.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12020
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00224"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2020-06-23"
     gene            complement(<1..2420)
                     /locus_tag="PWH45_RS24980"
                     /old_locus_tag="PWH45_24980"
     CDS             complement(<1..2420)
                     /locus_tag="PWH45_RS24980"
                     /old_locus_tag="PWH45_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612398.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_274753236.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREILSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIVENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLADTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPD"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            2515..3120
                     /locus_tag="PWH45_RS24985"
                     /old_locus_tag="PWH45_24985"
     CDS             2515..3120
                     /locus_tag="PWH45_RS24985"
                     /old_locus_tag="PWH45_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            3557..3652
                     /gene="dqlB"
                     /locus_tag="PWH45_RS24990"
                     /old_locus_tag="PWH45_24990"
     CDS             3557..3652
                     /gene="dqlB"
                     /locus_tag="PWH45_RS24990"
                     /old_locus_tag="PWH45_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_004206884.1"
                     /translation="MINLLIVVLRAVVAVSNAVIAVLELIRQYFD"
                     /name="dqlB"
     gene            complement(3703..5790)
                     /locus_tag="PWH45_RS24995"
                     /old_locus_tag="PWH45_24995"
     CDS             complement(3703..5790)
                     /locus_tag="PWH45_RS24995"
                     /old_locus_tag="PWH45_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="WP_004206885.1"
                     /translation="MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFT
                     MLLSILILPPLMVAFTDRKFRPPLRMPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRER
                     KKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFGTTGSGKTETFYGFIVNFLLWCRG
                     YCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVKQESIPAQSNSVN
                     LFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRTI
                     QKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHN
                     YMSRQFLKTLSLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGR
                     LYVTLQRMTISKDLGYQLEGKKEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQM
                     RSLEKMGVFSSQDHPSLARGANGEVDSLIANTRVKYFESIEDRKTFEILRETVGQDYYS
                     ELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQGVISFQDALVRSAAFYI
                     PDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIKLFGYD
                     KAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIE
                     M"
                     /name="conjugal transfer protein TrbC"
     gene            complement(5803..6753)
                     /locus_tag="PWH45_RS25000"
                     /old_locus_tag="PWH45_25000"
     CDS             complement(5803..6753)
                     /locus_tag="PWH45_RS25000"
                     /old_locus_tag="PWH45_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="WP_004206886.1"
                     /translation="MKLTVKTDRNTLYLSADGNYQEQALHSFPLSACHFGLGHDAGSGH
                     ILTHNTFSGEAVQLFRFENEYQAIEALNNIRKKLKQQRGLNAVRRLSNFIFWPVAALGF
                     IVSINGALMNMHQPAQPLQANSYAPAPGLQQPATAPEQLQVQAAPEAPKKLADNDLLSL
                     KDAVTSGRFTISLSTGHKHTLYVFSDPMCPHCRALEPRLQELSKRYNVEIFPVSVYGDL
                     RSMVRAAPVLDTGKDTRLKSWEDTINMGYTTMNPKIDDPKTAPDTTGARLVDANNMAFA
                     KFGFIGTPSIVADSGDVIPVSVVKDDARLQQAIGE"
                     /name="DsbC family protein"
     gene            complement(6764..8071)
                     /locus_tag="PWH45_RS25005"
                     /old_locus_tag="PWH45_25005"
     CDS             complement(6764..8071)
                     /locus_tag="PWH45_RS25005"
                     /old_locus_tag="PWH45_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059988.1"
                     /translation="MQNQPQRKGGGDDGLMIIGALLIMITVMWFGFRDYVIYGVCRLLY
                     WIYGVLPDAITPGNAAERRDMLLSAARYNHQVSLWDFISVMNDTASVLFPLFILIIGLF
                     VWVISRNPYYRLTRKLTIRNLPWVMVKNYPGIAHILARFGHLDQLLLNEDPEEARSAQS
                     PVEFAQEHNLIDTAKRRLRKKAAHKVFLSQVNLAPEGAALSLAPWEKALAASFVHLRFM
                     GDRPTAKSILDDLNRSCLRTKDGFPDYSLAAGFWTVASQCEAFREFAKGRRSSRSLLYA
                     LFDDDLQLPSANFRWLKGVDRTLWYALSSAGRGKFFIEGGGVIAWSQCESYRLTLDEEK
                     AAKFPPTVRAAVLGLELDLLHYRHIDRRDPAIPVAHWQEDVIPPFAFESDAEAEDEPAT
                     PPAAPHDTPNNSLRLTDDEPTPAKPQPATRPDHLEL"
                     /name="hypothetical protein"
     gene            complement(8071..8466)
                     /locus_tag="PWH45_RS25010"
                     /old_locus_tag="PWH45_25010"
     CDS             complement(8071..8466)
                     /locus_tag="PWH45_RS25010"
                     /old_locus_tag="PWH45_25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_004187436.1"
                     /translation="MVDAPPSIPLPTCMVEAAQIYHIPLRGFLALWLTEGGKPGMKNKN
                     KNGTYDYGAFQINTIWANKLASDFGVKAEQLQHDFCASAMASAYILKYNIILEGGDFWQ
                     GVGRYHSNTPARKAWYIGKVYQNSLRF"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(8571..8954)
                     /locus_tag="PWH45_RS25015"
                     /old_locus_tag="PWH45_25015"
     CDS             complement(8571..8954)
                     /locus_tag="PWH45_RS25015"
                     /old_locus_tag="PWH45_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1496 domain-containing protein"
                     /protein_id="WP_004206889.1"
                     /translation="MIADFFNGESEMKFTTKIALILAASVISCSAFAQSVSAPKTDSLL
                     SSILGEQVQTNQHLVQLLAKDQDAGITLTPEQKKTSCLYESHYYSSGAPLTVGKETLRC
                     TLSGEIPTWENVNEGNIVSVEGK"
                     /name="DUF1496 domain-containing protein"
     gene            9034..9687
                     /locus_tag="PWH45_RS25020"
                     /old_locus_tag="PWH45_25020"
     CDS             9034..9687
                     /locus_tag="PWH45_RS25020"
                     /old_locus_tag="PWH45_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="WP_004206890.1"
                     /translation="MIQSRNKYLQFMLVLLAAWAIAWGAMFVMGQAVLLYGLGKNTLFF
                     SHGAVLVYLLCVFLVYRRWVAPLPVVGQLSNVGALWLVGAMAVVYVGEFLLGKVLDLPA
                     EPFMTKLFADKSIPDVILTLLAVFILAPLNEEILFRGVMLNVFRSRYNWTMWLGALITS
                     LLFAAVHMQYQNLLTLAEMFLVGLITSAARIRSGGLLLPVLLHMEATALGLLLG"
                     /name="type II CAAX endopeptidase family protein"
     gene            9779..10036
                     /gene="pemI"
                     /locus_tag="PWH45_RS25025"
                     /old_locus_tag="PWH45_25025"
     CDS             9779..10036
                     /gene="pemI"
                     /locus_tag="PWH45_RS25025"
                     /old_locus_tag="PWH45_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="WP_004187429.1"
                     /translation="MHTTRLKKVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVE
                     PHRRPQYSLAELLAQCDPNAEISAEEREWLDAPAAGQEEI"
                     /name="pemI"
     gene            10038..10370
                     /gene="pemK-mt"
                     /locus_tag="PWH45_RS25030"
                     /old_locus_tag="PWH45_25030"
     CDS             10038..10370
                     /gene="pemK-mt"
                     /locus_tag="PWH45_RS25030"
                     /old_locus_tag="PWH45_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="WP_004206891.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVPV
                     TSGGNFARTAGFAVSLDGAGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMDDVLGC
                     LATILT"
                     /name="pemK-mt"
     gene            10463..10897
                     /gene="umuD"
                     /locus_tag="PWH45_RS25035"
                     /old_locus_tag="PWH45_25035"
     CDS             10463..10897
                     /gene="umuD"
                     /locus_tag="PWH45_RS25035"
                     /old_locus_tag="PWH45_25035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187425.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="WP_004187425.1"
                     /translation="MALDLVYPRPSVEGDALPFFAEPVAAGFPSPAAGYEANELNLHDY
                     CVRRPAATYFLRVSGDSMRDARIHDGDVLVVDRAEEPRHGSIVIASIDNEFTVKQLQLR
                     PYPCLMPLNPAFPPIRFDPEALQIWGVVTFTVMRHRPCTP"
                     /name="umuD"
     gene            10912..11391
                     /locus_tag="PWH45_RS25040"
                     /old_locus_tag="PWH45_25040"
     CDS             10912..11391
                     /locus_tag="PWH45_RS25040"
                     /old_locus_tag="PWH45_25040"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpB/MucB/SamB family protein"
                     /protein_id="WP_274753237.1"
                     /translation="MYCSCEEAFRPDLRGRPVVVLSNNDGALVAVNRAAKALGVRRGEP
                     YFRCRRLLEQHNVAVFNSNYTLYASFSARFATVVESLAPRTFVYSIDELFADASHMEGV
                     MTPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAAKTWPATGGLWRSVIL"
                     /name="ImpB/MucB/SamB family protein"
     gene            11406..>12020
                     /locus_tag="PWH45_RS25045"
                     /old_locus_tag="PWH45_25045"
                     /pseudo=""
     CDS             11406..>12020
                     /locus_tag="PWH45_RS25045"
                     /old_locus_tag="PWH45_25045"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="binds processed UmuD protein to form functional DNA
                     pol V (UmuD'2UmuC); involved in translesion polymerization;
                     incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V subunit UmuC"
                     /name="DNA polymerase V subunit UmuC"
     Typing          complement(3707..5790)
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.424"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atatcaggcc agttcgcaac gataagatcc tcccgggctt ttccacctac caacttgcgt
       61 aactccctgg gggtcgtatc gggattaaat acgtacaacc gcttcgatgg cagatccctg
      121 attcgcagaa cgagattgta gccgagcagg ctactggctc cgaacaaatg gtcggtgaat
      181 cctgctgtat cggcatactg ttcgcgaaca tggcgaccga cctcgttcat cagtagtcca
      241 tcgagaatat acggtgcctc gctcacggtc gccgggatcg actgacaagc gaatggcgcg
      301 aactggtcgc ttacgtgagt atacgctttg aggccgggaa cagaaccata tttggcattg
      361 accatgttca tggcttcgcc atgccgcgct gtcgggaaaa actgaccatc gctcgatgct
      421 gacgtgccca tcccccagac gcgtgacatc ggcagtttac cctgcgcggc caccacaatt
      481 gccaatgcct ggttcatggc ttcgctttca acatgccagc gggcaaggcg tgagagctgc
      541 cagtaatcat gcgtgtttgt agcttccgcc atcttacgca ggcccagatt gagcccttca
      601 gcgagcagga cgttgagcag accgatccgg tcgcgacatg gagccccggt tctcagatgg
      661 gtaaacgcat ctgtgaaacc aagggctgca tcaacttcaa gcagcatgtc ggtaatccga
      721 acggacggca ttcggcgata cagatccagt atgagtgcct cggcaccatc cggcacgtct
      781 gctgtcaacc tgtcgatccg caacgttcca tcttctatgc taccgtgcgg aatagtgccg
      841 ttacgggcag cccgggccag ccgcttaaga gcgatcgtga gtcgcgcctt tctgtctgcc
      901 agccaatcct gtgggttgga aggcacggcc agttttgcat tttcctgcgc cgcgatcatc
      961 ggcaccagta cctgcttgag gtcaccatag cggcgcgaat gagcgagcca gacatctccg
     1021 gaacgaaaag catcccggag gtgaaagagt accgccactt cccaaagacg ggtatctcct
     1081 ttttcctgag ctcgtaaatg acggttccat ttggagctgg gccgcaggaa acgcctttct
     1141 ggcgatgcaa cacctttcat ctctccgatc gacaaagctg ctgctaccaa tggtccggcg
     1201 accggcgcgg cttcgagctt cagacagcgc aacatgcggg gcgcataacg acgaaagcga
     1261 tggtatccct gcccgacata tgcaagaggc tcatcggcta gcgtgttgct gagttgagtc
     1321 cctgtcgcta ccagttgagc gagccggtcc catgcaaccg aactggcgac agccatctcc
     1381 agcggggttc cgtcactgcg ggcctcaagc aacgaagctc ccagcgcggt gaaggtacgg
     1441 atcgtatccg cgagtgtggc tttagagccg gaaattgttt cgtcatgctg gcgcttcgct
     1501 tcccgccagg tttttcctac gatcctgtca tgggtttcga ctatggcatc agcaatcgcc
     1561 gcttcccact ccacaacaca gacggcaagg atcgcccagc ggcggtccga agtgatgtca
     1621 cgcaaaccgt cggtgaagta gcgttcaccc tgccgacgca gccgggcaat gcgatgggca
     1681 ggtatgctgg ccaaagcact atgattgata ttcagggtac gcagaaattc gagcctgtcg
     1741 agcaaacggt tagcagcagc cgagttgtta ccaacctcga agttgcgaag ccagatgaaa
     1801 cgactgatat tgccggcgag catttcactc agaagtttgt ccaggtgatc gcgaacatcc
     1861 gctgttaaat tttccacaat ccgcgtttca atccgccgct cagcggcgac cagagcatcc
     1921 gcgcacaagc gctcgattgt cgatactgcg ggcagaatgg tggaagtttc ccgacaccgc
     1981 acaataaaac gatgagcaag atcctcgttt gatctggcat cttcggcctg gccgaaagtc
     2041 cactcccgca gatcacgggc accacggccc gtgaaggtct tgtagccgta aatttcgcgc
     2101 agcgtgtcca tgtgctgctg acgggtttgg cgccgtgtgg cataagtgag aagcgcatca
     2161 gccggaactc caagctgagc accgacgaag gaaaggattt cacgcgggat catctcacca
     2221 ggagccagtg cacggcccgg atatcgtaag gcacaaagtt gcagggcaaa gccaatcctg
     2281 ttttccggtc tgcggcgctg cctaatgttt tccaggtcat catcgcccag cgtgtagaac
     2341 ttcagtagcg acagttcgtc cgtgggcaga tcgaacagcg ctgctcgctg ccgttcggtg
     2401 aaaatatggc gtcgtgacat acaaattcgt cccttttgaa gtatagtctg ttttggacaa
     2461 cagccagccc atataaatca gggcgttccg atacaaaaat ccaggagggt tcaattggga
     2521 catcgtgccg ccatttactg ccgggtttca acagcggatc agtcttgtga acgccaggaa
     2581 tttgatctgc gagccttcgc cggccgtgcc ggctacgacg tggtgggaat atttaaggaa
     2641 acaggttcag gaactaaact cgaccgggcc gagcgaaaga aagtcctggc gcttgcccag
     2701 tccagacaaa ttgatgcaat cctggtcact gagctttccc ggtgggggcg ctcgacgctc
     2761 gatctgctca atacgctacg tgaactggag aactggaagg tttccgtgat agccatgaat
     2821 ggaatggcgt tcgatctttc gtcgccgtat ggacgaatgc tggcgacgtt tctttccggc
     2881 attgcggagt ttgagcggga tctcatcagc gagcgggtca agtcaggcct tgctgttgcg
     2941 aaggcacgtg gtaagaggct tggtcgtcag gccggagtgc gaccaaaatc agaccgactt
     3001 ttgcctaagg tggttgcgat gagggccgag ggacgcagct atcgctggat cgcacgcgag
     3061 ctcggtatca gcaagaatac cgtcgctgac atcgtgcaac gacacagagc taacgcttag
     3121 ggtgtcattt cgccctcagc cggaaccgac cccgtgaagg ccacagagag cgtttcaggc
     3181 gcttatttga ggaaatgcga tttctgccag aatggaggca caaaaaaacc cgactggtga
     3241 gatcgggttt ttatgttttg ccccggatgg aggcgggaac aaccaaaaca attgacggtt
     3301 aaatattacg ccatgagtgc agtaaatgca aaccaccggg aatagggtat actgcgcggg
     3361 tcaatctgtt gacgttcttc tccagccccc cgcttatgcg gcatgaacgg attaaagtcc
     3421 tggttgaaaa acaggtggtg ctgtttcaat gccctggctg gagtgggaac ggccaaaaca
     3481 attgacgtga ggtagccgct gtgacaggca gtgtaagggc gatcagcggt gttcctgctc
     3541 tgtatggagc taacggatga ttaacttgtt aatcgtcgtt ctgcgggcag ttgttgcagt
     3601 gtctaacgct gtgatagcgg tcctggagct gatccggcag tattttgact gaccggaaac
     3661 gtaactaaag gcggggggac cactccccgc cttttaacag ggttacatct cgatcatcgg
     3721 gaacagatcg tcttcctggt ggtaaagcac gtcgtccgtt tccgttccat caagccattc
     3781 agcgaacagt tcaaacaggc acaccgacat ttcctccggt gaaattgcgc cacactgcag
     3841 ctcgtagaag acctcaattt tttccagcag gctgttggcc gctttgtcgt agccgaacag
     3901 cttgatcggc gggaatcgca ttgcatccgg gttggtttcc gccagttcat catccttgcg
     3961 ttccggataa atgctgtaca gcgttgccgg cgatggcggc aatacctcga taaagcggtt
     4021 tatccgcatc ggcagttcgc tggaaaactt ctcattgtcc ggaatgtaaa acgcggcgct
     4081 gcgtaccagg gcatcctgga aagaaattac cccctgacct tcggtctgct tacgtagctc
     4141 acgaatgttc acgcggtcct tttcacggat ttggtacaga tccccttccc ggaccgtttt
     4201 ggaaaacgtg ccgtgttcgg cttcctgccc ggagagctca gaataataat cctggccgac
     4261 ggtttcgcgc agtatttcaa aggttttccg gtcttcaatc gactcaaaat atttcacgcg
     4321 ggtattggcg atcagcgaat ccacctcgcc gttcgcgccg cgggcgagcg acgggtgatc
     4381 ctgggaggaa aatacgccca ttttttccag tgaacgcatc tgcgcactca gcgtatccat
     4441 accgctggtg aaatactggc ccagctcgtc ataaatcagc ccgtatggcg cctgattatt
     4501 cagcgcgtga gtgagcagga cttcctcttt tttcccttcc agctggtagc ccaggtcctt
     4561 actgatggtc attcgctgca gcgtcacata taaacggcca agcgtggccg cttcaccgcg
     4621 tgacagctcc agcgacggga tcatgacgat gagaatgcgg tcattgtgga aaatatcgtc
     4681 catccggata tcgccgctgt cttccggaaa aacatggcca taagtttcat tgaacaggga
     4741 gagcgtcttc aggaactgac ggctcatgta gttatgctgt tcgaaggcgc gggcttccca
     4801 cgtttccggg tattccgcgt tggccagcag gaagcctggt gtattttcca gataactttc
     4861 cagcgcggcg taaccctcgc tgtgccagcc gtttttcttt gcctccacgt acagcgcagc
     4921 cattgccggc agcgacatgt ttttctgaat agtgcgctgt gacaaaagca gttcgccgcg
     4981 ggcgcgctta taacacaggg cattgatcag cgccgacatc atggcttttg ccgtatcctg
     5041 ccactgtgcc gaatccccgc ccacctgggg gagcatggat tccatcaact ggatgatgaa
     5101 ggtcggcggg gcaacgctga agaggttgac tgagttggat tgcgcgggga tgctttcctg
     5161 cttcaccagg ttcacgaacc tgtcaatact gccggtcagc aggttgagaa cgtaataatc
     5221 atcctcacgg ccgaaacggc gcgccagcga ccaggtggcg aaggccagct tgttatctgc
     5281 tttaccgtct gagaggcagt agccacggca ccacagcagg aagttaacga taaacccata
     5341 gaaggtttcg gttttacccg acccggtggt accaaaaaat accatgtggc gcagcaggtc
     5401 cgtcatgttc agccacagct cgcggccgaa caggcgtccc cgctcatacc cgacatataa
     5461 aattcccttc gctttctttc gctcccggac cttatgcggg atgcgaatgg gccccagacg
     5521 gtattccgcc tgggtttccg tggtcagggt gtcgtcgaac atttcgcagt ctttcggcat
     5581 tcgcagcggt ggccggaatt tacgatccgt gaatgccacc atcagcggcg gaaggatgag
     5641 gatgctcagc agcatggtaa aggggagaaa cagacccagc acggtcagta tggccagcag
     5701 gccaaactgt acgttcggag tgaggaacca ctccatgaac gaaccatctg ggcggaggac
     5761 ttttttactg tctacagatt cctgttgcat agcggctccg ggttattctc ctattgcctg
     5821 ctgcaggcgg gcatcgtctt tgacgacgga aaccgggatc acatcacccg aatcggcaac
     5881 gatggacggg gtaccgataa acccgaattt cgcaaatgcc atattgttcg cgtcaaccag
     5941 gcgggcgccg gtggtatccg gggctgtttt cggatcgtca attttcgggt tcatggtggt
     6001 gtatcccatg ttaatcgtgt cttcccagct tttcagtcgg gtatctttcc cggtatcgag
     6061 tacaggagcg gcgcggacca tcgagcgcag gtcgccataa acggacacgg ggaaaatctc
     6121 gacgttatag cgtttgctca gttcctgcag tcggggttcc agcgcgcggc agtgcgggca
     6181 catcggatcg gaaaacacat acagggtgtg tttatgcccg gttgaaagcg agattgtaaa
     6241 gcgtccgctg gtgacggcat ctttcagtga cagcaagtcg ttatcagcca gttttttagg
     6301 ggcttcaggt gctgcctgaa cctgcagctg ctccggcgca gtggcaggct gctgcagacc
     6361 cggcgcggga gcatacgagt ttgcctgcag tggctgtgcc ggctgatgca tattcatcaa
     6421 cgcgccgttg atggagacga taaagcccag cgcggcgact ggccagaaga taaagttaga
     6481 cagccggcgc acggcattga gtccgcgctg ctgtttcagc tttttacgga tattgttcag
     6541 cgcctcgatg gcctgatatt cgttttcaaa gcggaacagc tgcaccgctt caccagaaaa
     6601 cgtattgtgc gtcaggatgt ggccgctgcc ggcatcatgg ccaagtccaa aatgacaggc
     6661 tgacaggggg aaggagtgca gggcttgttc ctggtagttt ccgtcagcgg aaagataaag
     6721 tgtgttgcga tcggttttta cggtgagttt catacttcct cgcttagagt tccaggtgat
     6781 cgggtcgggt tgccggctga ggtttagccg gcgtcggttc atcgtctgtc agtcgcagac
     6841 tgttgttcgg ggtgtcgtgc ggagctgcag gcggcgtagc cggctcgtct tccgcttcag
     6901 cgtcagattc aaatgcaaat ggcggtataa cgtcttcctg ccagtgagcg accggaatgg
     6961 cgggatcgcg gcggtcaata tggcggtaat ggagcagatc cagctcaagg cccagcacgg
     7021 ccgcccgcac ggtcggcggg aatttcgccg ccttttcttc atcgagcgtc agccggtaac
     7081 tttcgcactg actccaggca ataacaccac ctccctcaat gaagaacttc ccgcggccgg
     7141 cgcttgaaag tgcataccat aacgtgcggt ccacaccttt aagccaccgg aaattagcgg
     7201 atggcagctg caggtcatca tcgaacagcg cgtagagtag cgaacgggag ctgcggcgcc
     7261 cctttgcaaa ttctcggaac gcttcacact ggctggcgac ggtccagaac ccggcggcca
     7321 gcgaataatc agggaaaccg tctttagtcc gcaggcagga acggttgaga tcgtcgagaa
     7381 tggatttagc cgttggccgg tcgcccataa accgcagatg tacaaacgag gctgcgagcg
     7441 ctttttccca gggcgccagg gaaagggccg ccccttcggg ggccaagttt acctgtgaca
     7501 ggaagacttt atgtgcggct tttttacgga ggcggcgctt agcggtatca atcaggttat
     7561 gttcctgggc aaactcaacg ggtgactgcg cgctgcgtgc ttcttccgga tcttcattca
     7621 gcagcagctg atcgaggtgg ccaaaccggg cgaggatatg agctatcccc ggatagtttt
     7681 ttaccatcac ccagggaagg ttccggatgg tcagtttacg ggtcagacgg tagtaaggat
     7741 tgcgcgagat aacccagacg aacaacccga tgattaaaat gaacagggga aagagcacgc
     7801 tggccgtgtc gttcatcacg ctgataaagt cccacagcga aacctggtgg ttataccgcg
     7861 ccgccgacag cagcatgtcc cggcgctcgg ccgcattacc cggtgtaatg gcgtccggca
     7921 gtacaccgta gatccagtaa agcaggcgac agactccgta aatcacataa tcacgaaagc
     7981 caaaccacat cactgtgatc atgatgagaa gggccccgat aatcatcagg ccatcatccc
     8041 ctcccccttt ccgttgtggc tggttttgca tcagaacctc agagaatttt ggtaaacctt
     8101 cccgatatac caggccttac gcgccggcgt gttggagtga tagcggccga caccctgcca
     8161 gaaatccccc ccttcgagaa tgatgttgta tttgaggatg taggcactgg ccattgcaga
     8221 ggcgcagaag tcatgctgca gctgctccgc ttttactcca aaatcggacg ccagcttatt
     8281 cgcccagatg gtgtttatct ggaatgcgcc gtaatcgtaa gtgccgtttt tgtttttatt
     8341 tttcattccc ggttttccgc cttcagtcag ccacaaggcg aggaatccac ggagaggaat
     8401 gtgataaatc tgtgctgctt caaccatgca ggttggcagc gggattgagg gcggggcatc
     8461 aaccataaca atctcctaat gatttctatt tcgagtaaaa aatataccat caaaaggaga
     8521 tttttttaaa tcattttggt gcggataatt gagaggctgt caaagggatt ttatttgcct
     8581 tcgacagaga caatattgcc ctcgttcaca ttttcccatg tcgggatttc gccactcagc
     8641 gtgcagcgga gtgtttcttt tccgacggta agtggtgcgc cactgctgta gtaatgactt
     8701 tcatacaggc aagaggtttt cttttgttcc ggggtcagtg taataccggc atcctgatcc
     8761 tttgccagca gttgtacaag atgttggttt gtctgtacct gctcacccag aatgctgctc
     8821 agtaaagaat cggttttcgg cgcggatacg ctctgagcaa aggcactgca actgattacg
     8881 gatgcagcca gaataagagc aatcttcgtg gtgaatttca tttcactttc tccattaaaa
     8941 aaatcggcaa tcattttctg tatcatttaa attaggtctg aaagccggcc tgagtgtcaa
     9001 gccgattctg tttaaaaatt aacaggggat aaaatgattc agtcacgtaa taaatatctg
     9061 cagtttatgc tggttctgct ggcagcctgg gccatagcct ggggggccat gtttgtcatg
     9121 gggcaggctg ttctgctcta tgggctgggg aaaaatacgc tgttcttcag tcatggtgct
     9181 gtactggtgt acctgctgtg tgttttcctg gtataccgcc gctgggttgc tccgctaccg
     9241 gtcgttggcc agctgagcaa cgttggcgca ctgtggctgg ttggcgcgat ggccgtggtg
     9301 tatgtcggtg aatttctgct gggtaaagtg ctggacctgc ccgctgagcc atttatgacg
     9361 aaactttttg ccgataagtc catacccgat gtgatcctga cgttgctggc cgtctttatc
     9421 cttgccccgc tgaatgagga aatcctgttc cgtggtgtca tgctgaacgt cttccgttcg
     9481 aggtacaact ggacgatgtg gctgggcgcg ctgataacgt ctttgctgtt tgccgccgtg
     9541 catatgcagt atcagaacct gctgaccctg gcagaaatgt tcctggtggg gctgattaca
     9601 tcagctgccc ggatcagaag tggcggcctg ctgctgccgg tattgctgca tatggaagct
     9661 accgcgctgg gtttactgtt gggctgaaag ttatattttt attaaacatt gtgcgttaaa
     9721 ggctggtatg tttttttagt gaatgttata tttaaatata acttttatgg agggaaaaat
     9781 gcataccact cgactgaaga aggttggcgg ctcagtcatg ctgaccgtcc caccggcact
     9841 gctgaatgcg ctgtcgctgg gtacagataa tgaagttggc atggtcattg ataatggccg
     9901 gctgattgtg gagccgcaca gacgcccgca gtattcactg gctgagctgt tggcacagtg
     9961 cgatccgaac gctgaaatct cggcagaaga acgtgaatgg ctggatgcgc cggcggctgg
    10021 tcaggaggaa atctgacatg gaaagagggg aaatctggct tgtctcgctt gaccctaccg
    10081 caggtcatga gcagcaggga acgcggccgg tactgattgt cacgccggct gcttttaacc
    10141 gcgtgacccg cctgcctgtt gttgtgcccg tgaccagcgg aggtaatttt gcccgcacag
    10201 caggctttgc tgtgtcgctt gacggcgccg gcatacgtac caccggcgtt gtgcgttgcg
    10261 atcaaccccg gacgatcgat atgaaagccc gcggcggcaa acgactcgaa cgggtgccag
    10321 agactatcat ggacgacgtt cttggctgtc tggccaccat cctgacctga ccccagcgtg
    10381 ccggcatgaa atatgccggc attgcctgtt tcctgaagcc tgcaatatac tgtatgtata
    10441 aacagttgtt ggaggctaaa ttatggcgct ggatctcgtt tatccccggc cttcagtgga
    10501 aggtgacgca ctcccttttt ttgccgagcc cgttgcggcc ggctttccca gcccggccgc
    10561 aggctatgag gccaatgaac ttaacctgca tgattactgc gtgcggcgtc cggccgcaac
    10621 gtacttcttg cgtgtgtccg gcgattctat gcgtgatgcc cgcattcatg atggcgatgt
    10681 gctggtcgtc gatcgggcag aagagccccg gcacggcagc atcgtgattg ccagcatcga
    10741 taatgaattt acggtcaagc agctgcagct ccgcccttac ccctgcctga tgccgctgaa
    10801 tccggctttt ccgccaatca ggtttgatcc agaggcgctg cagatatggg gtgtggttac
    10861 gttcactgtc atgagacacc gcccgtgtac gccttaatcg atgtcaattc gatgtactgc
    10921 agctgcgaag aagcgttccg ccctgattta cgaggtcgcc ctgtggtcgt tctcagcaac
    10981 aacgacgggg cgcttgttgc ggtcaatcgc gcggcgaaag cgcttggcgt gcgccgcggg
    11041 gagccctatt tccgctgccg ccgcctgctg gaacagcata acgttgccgt attcaattcc
    11101 aactacaccc tttacgcctc attctccgcc agatttgcaa ccgtggtgga gagtctggcg
    11161 ccgcggacat ttgtttactc aattgatgag ctctttgcgg atgcctctca tatggaggga
    11221 gtgatgacgc cgcaggcgtt tggccagctt ttgcgggctg aagtgctgcg ccagacaacg
    11281 ctcacctgtg gagtcggcgt cgcgccaacg aagacgctgg ccaagctgtg caaccatgcc
    11341 gcaaagacgt ggcctgctac cggggggttg tggcgctcag tgatcctgta agactgaaaa
    11401 agctgatgag cctggtggcg gccggcgatg tgtggggcgt tggccatcgt accgaaaaag
    11461 ggctggcggc aatggggatc aaaacggcgc tggcgctcgc agagatggat atccgtcttg
    11521 cacgccggct gtacggtgtg acgctggaaa ggaccattcg tgagctgcgt ggcgaggcct
    11581 gctttgccct ggaggaagga accggcgcga aacagcagct tgttgtatcc cgttcgtttg
    11641 gagcacgggt cacgcaactg gcgcagatgc agcaggcggt gactaaatat gccacacggg
    11701 caggggaaaa gttgcgccag gaaaaccgcc tggcgaaggt ggtaacggtc tttatccgca
    11761 gcagtgctta tgacgccggc ggtgtgccct acagtaacca ggctacggaa gtgcttcccg
    11821 tgccaagcca ggatacccgc gacatcatcg ccgcggcgca gctgggactg tcgcgtatct
    11881 ggcgtgaggg cgtgcggtat gcaaaagccg gcgtgatgct cagcgatctg cagggatatg
    11941 aaacccagct ggatctgttt tcgccggctg ctgtgcggcc gggcagcgaa aaactgatgg
    12001 ccaccatcga caaaataaac
//
