LOCUS       NZ_CP118386            12166 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00224 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP118386
VERSION     NZ_CP118386.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907159
            Assembly: GCF_028872235.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 12166)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00224
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12166)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease Submission Group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028872235.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 07:03:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,023
            CDSs (total)                      :: 4,908
            Genes (coding)                    :: 4,818
            CDSs (with protein)               :: 4,818
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 37 of 90
            Pseudo Genes (incomplete)         :: 53 of 90
            Pseudo Genes (internal stop)      :: 18 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            Pseudo Genes (short protein)      :: 1 of 90
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118386.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12166
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00224"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2020-06-23"
     gene            join(11145..12166,1..4)
                     /gene="istA"
                     /locus_tag="PWH45_RS24930"
                     /old_locus_tag="PWH45_24930"
     CDS             join(11145..12166,1..4)
                     /gene="istA"
                     /locus_tag="PWH45_RS24930"
                     /old_locus_tag="PWH45_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490293.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="WP_004199214.1"
                     /translation="MLTQEQTVEIRVLARQGHSIRHIARTLGVSRNTVRRYLRDPSVAR
                     YQPREPRPTKLGPFESYLRQRVEQAHPIWLPATVLNREIRAQGYGGGLSLLRAFLATLK
                     PARREAGPAVRFETEPGRQLQADFVVLRRARSPMSAFVATLGYSRMTFVTFVPDESFES
                     VRDSLLLAFDYLGGVPREVLFDNMKTVVLERDAYGDGKHRFHPGLLQLADDLGFRIRLC
                     RPYRAQTKGKVERFNRYFRESFYNPLLTRMKGTGLLLDCAAANRRVRDWLADEANVRVH
                     ATLNERPIDRWRQEREHLQPLPSRVRRDEAPLLDNSLRPVPLESLQHPLSVYDAIGEAC
                     R"
                     /name="istA"
     gene            1..780
                     /gene="istB"
                     /locus_tag="PWH45_RS24935"
                     /old_locus_tag="PWH45_24935"
     CDS             1..780
                     /gene="istB"
                     /locus_tag="PWH45_RS24935"
                     /old_locus_tag="PWH45_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISKpn7 family helper ATPase
                     IstB"
                     /protein_id="WP_004152394.1"
                     /translation="MNLQHERIDALCQQLKLERVATHYPVLAQQAVREDFSFTDFLEHL
                     LRHEAGCRQQRSRELLTRMACFPGIKTLEDYDFSFNPGVPKALVQELGSLAFVERAENV
                     VLIGPSGIGKTHLAIALGYKAAQAGVKTRFITAADLMLQLSTAQRQGRLKEYFNRAILG
                     PRLLVIDEIGYLPFGREEANLFFQVVAKRYEKGAMILTSNLPFSQWADAFAGDTTLTAA
                     MLDRLLHHAHILTLSGESYRLKDKRKAGVVRKNSKPE"
                     /name="istB"
     gene            1167..2048
                     /locus_tag="PWH45_RS24940"
                     /old_locus_tag="PWH45_24940"
     CDS             1167..2048
                     /locus_tag="PWH45_RS24940"
                     /old_locus_tag="PWH45_24940"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-3"
                     /protein_id="WP_004152396.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKYSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-3"
     gene            complement(2298..3617)
                     /locus_tag="PWH45_RS24945"
                     /old_locus_tag="PWH45_24945"
     CDS             complement(2298..3617)
                     /locus_tag="PWH45_RS24945"
                     /old_locus_tag="PWH45_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /protein_id="WP_004152397.1"
                     /translation="MAASYLPYRPDQSYLLPPSLGEWLPEGHLAYFISETVDTLDLSAF
                     HARYAGGGPGNQPFHPAMMVKVLIYGYASGVFSSRKLARKLYEDVALRVLAAGNFPAHR
                     TLSDFRALHLTELENLFVQVVQLARECGLVKLGTIAVDGTKVKANASRHKAMSYKRMKP
                     AEDELHCEIKALLDRAKATDDQERNEPELDIPAEISRREKRLEAIQAAKARLEARQREA
                     DQARGRSEDDGRRPRHPDGSDKGGGSYKREFGVPDDRDQESFTDPDSRIMKHAGGGSEQ
                     SYNGYTAVDAEHQIIVAAELTNCAADSQALLGMLAAVQANTGEMPAQTLADAGFRSEAV
                     LAKVADHHGDVIVALGREGREDAKVNAKTHPHTAAIAAKLKTEQGDAAYRRRKSIVEAP
                     NGWIKAVMGLRQFSMRGLDKVQAEWKLVCMALNLRRMAYL"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            3990..4217
                     /locus_tag="PWH45_RS24950"
                     /old_locus_tag="PWH45_24950"
     CDS             3990..4217
                     /locus_tag="PWH45_RS24950"
                     /old_locus_tag="PWH45_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224019247.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(4265..4453)
                     /locus_tag="PWH45_RS24955"
                     /old_locus_tag="PWH45_24955"
     CDS             complement(4265..4453)
                     /locus_tag="PWH45_RS24955"
                     /old_locus_tag="PWH45_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4708..5010)
                     /locus_tag="PWH45_RS24960"
                     /old_locus_tag="PWH45_24960"
     CDS             complement(4708..5010)
                     /locus_tag="PWH45_RS24960"
                     /old_locus_tag="PWH45_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_023207139.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGVHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            complement(5318..5431)
                     /locus_tag="PWH45_RS24965"
                     /old_locus_tag="PWH45_24965"
     CDS             complement(5318..5431)
                     /locus_tag="PWH45_RS24965"
                     /old_locus_tag="PWH45_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072202106.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(6184..7899)
                     /gene="tnpR"
                     /locus_tag="PWH45_RS24970"
                     /old_locus_tag="PWH45_24970"
     CDS             complement(6184..7899)
                     /gene="tnpR"
                     /locus_tag="PWH45_RS24970"
                     /old_locus_tag="PWH45_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family resolvase TnpR"
                     /protein_id="WP_004152391.1"
                     /translation="MKTGQTTASSSTPVGFPDADSLAALRGWYAGLSSRAAVEHYLAAR
                     KAAGQSARGMLGHIRRQLIVFANSRHRPDLADVFQHAPEERQGRAGAVERAIRLLPTLP
                     APKPQISDSIDQWLSPRTVRALQAYGIHTLSALTVRIPRRRGWWKAIEGLGMTGARQVE
                     AFFAAHPQLTERARALIPIIQPSGIVPWEQLRLPNEVDGSQGNFRAPRNACTLNATNDY
                     EAVQAWLALHETVATQRAYRKEAARLILWAIVERGRALSSLTTDDAIAYRAFVRHPTPR
                     ERWVGPPRPRDSVEWRPFSGGLSARSAAYALTVLSALFRWLIEQRYVLANPFAGVKVRG
                     HALRPALDTARGFTEGEWLLLRAIADGLEWSYGWSEPAAQRLRFMLDFGYATGLRASEL
                     VGAVLGNVRLDEHGDHWLHLVGKGGKPGKVALPPLAYTALDQYLAQRQLPVSRERWNPK
                     TPLVGSLGEDGNAGITGPHLWCVLRRFFTQAAEAIEADHPVAAEKLRRATPHWMRHSHA
                     SHALARGAQLTAVRDNLRHASIATTSMYLHGDELERARQMRQAFGARHVKRGVD"
                     /name="tnpR"
     gene            8009..11038
                     /gene="tnpA"
                     /locus_tag="PWH45_RS24975"
                     /old_locus_tag="PWH45_24975"
     CDS             8009..11038
                     /gene="tnpA"
                     /locus_tag="PWH45_RS24975"
                     /old_locus_tag="PWH45_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152392.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family transposase"
                     /protein_id="WP_004152392.1"
                     /translation="MSKKNKLLSVLSDAEQEALYGLPEFDDAQQLEYLAVTETELALAN
                     SRPSLHAKVYCLLQIGYFKAKHAFFSFDWDEVEDDCAFVLSRYFHGEVFEPKAITKHER
                     YTQREQIAKLFGYWPWSAAFLPQLEQQAARTVRRDVTPGFVAAELIVWLNEHKIIRPGY
                     TTLQELVSETLSAERRRLGNLLEQALDESAKAALSQLLVRDDTLSQLAALKQDAKNFGW
                     RQMVREREKRATLEPLHAIAKTLLPKLDVSQQNLLYYASLANFYTVHDLRNLKADQTQL
                     YLLCYAWVRYRQLSDNLVDAMAYHMKQLEDESSAGAKQSFADEQVRRQQETPRVGRLLS
                     LYVDDSVADPTPFGEVRQRAYKIMPRDTLQTTAQRMSVKPASKLALQWQAVDGLADRMR
                     RHLRPLYVALDLSGSNPDSPWLAALAWAKGVFAKQQRLSQRPLDEWPAATLPKRLRPYL
                     LTFDADGKPTGLHADRYEFWLYRQIRKRLQSGELYLDDSLQHRHFSDELVEMDRKIDVL
                     AKMEIPFLQQPVHAQLDALTAELRTQWLAFNRELKQGKLTHLEYNKDTQKLTWRKPKSE
                     NEKAREKAFYEKLPFCDVADVFRFVNDECKFLSAFTPMQPRYAKKVADADSLMAVIIAQ
                     AMNHGNHVMARTSDIPYHVLDSGYQQYLRQATLHAANDCISNAIATLPIFPYYSFDLET
                     LYGAVDGQKFSVERPTVKARYSRKYFGRGKGVVAYTLLCNHIPLNGYLIGAHDYEAHHV
                     FDIWYRNTSDIMPTAITGDMHSVNKANFAILHWFGRRFEPRFTDLNAQLKELYCADDPA
                     QYQACLIRPVGKIDCDLIHREKPNIDRIVATLGLKEMTQGTLIRKLCTYTTTNPTRRAI
                     FEFDKLIRSIYTLRYLRDPQLERNVHRSQNRLESYHQLRSAIAQVGGKKELTGRTDIEI
                     EISNQCARLIANTIIFYNSAILSRLVTKYEAAGNSKALALITKISPAAWRHILLNGHYT
                     FQSSGKTIDLDAIVAGLELE"
                     /name="tnpA"
     ARG             1168..2045
                     /name="blaKPC-3"
                     /gene="blaKPC-3"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-3"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          5794..5966
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.954"
                     /coverage="100.0"
                     /evalue="2.04e-62"
     Typing          complement(5323..5869)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.625"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 atgaacctcc agcatgagcg gatcgacgcc ctgtgccagc aactcaaact cgaacgggtg
       61 gcaacgcatt accccgtcct ggcgcagcag gcggtgcgcg aggacttcag cttcaccgac
      121 ttcctggaac acctgttgcg gcatgaagcg gggtgtcgcc agcagcgcag ccgcgaactg
      181 ctgacgcgga tggcttgctt cccgggcatc aagaccctgg aggactatga cttcagcttc
      241 aaccccggcg tgcccaaggc actggtgcag gagctgggca gtctggcgtt tgtcgaacgg
      301 gccgagaacg tggtgctgat cggaccctct ggcatcggca agacccacct cgccatcgcc
      361 ttgggttaca aggccgcgca agccggggtc aagacacgct tcatcacggc agccgacctg
      421 atgctgcaac tgagcacggc gcagcgacaa gggcggctga aggaatactt caaccgcgcc
      481 atcctcggtc cacggctgct ggtgatcgat gagatcggct acctgccttt cggacgcgag
      541 gaagcgaacc tgttctttca ggtggtggcg aagcgctatg agaaaggggc gatgatcctg
      601 acctcgaacc tgccgttcag ccagtgggcc gacgcgtttg ccggcgatac caccctgaca
      661 gccgcgatgc tggatcggct gctgcaccat gcccatatcc tgaccctgag cggcgaaagc
      721 taccgcttga aggacaagag gaaggcggga gtggtcagga aaaattccaa acccgaatga
      781 tccaggtggg tcagtattac tttggtgatt caggggtaaa gtgggtcagt tttcagttgg
      841 tgttgacacc ggcgtaccct cggtgctatc ttcgcgcccc aatagtcggg gcttggccag
      901 gacttcctga ggccgtccgt aacgtggatg ccgaggtcag gcgaggtggc cgacccatga
      961 acgccgacct gattcgtttt tcaatagcgc tggacgttgt ggtgccaggg acttaccaac
     1021 ccgatgtgtg cccatccggg gcagttacag ccgttacagc ctctggagag ggagcggctt
     1081 gccgctcggt gataatccca gctgtagcgg cctgattaca tccggccgct acacctagct
     1141 ccaccttcaa acaaggaata tcgttgatgt cactgtatcg ccgtctagtt ctgctgtctt
     1201 gtctctcatg gccgctggct ggcttttctg ccaccgcgct gaccaacctc gtcgcggaac
     1261 cattcgctaa actcgaacag gactttggcg gctccatcgg tgtgtacgcg atggataccg
     1321 gatcaggcgc aactgtaagt taccgcgctg aggagcgctt cccactgtgc agctcattca
     1381 agggctttct tgctgccgct gtgctggctc gcagccagca gcaggccggc ttgctggaca
     1441 cacccatccg ttacggcaaa aatgcgctgg ttccgtggtc acccatctcg gaaaaatatc
     1501 tgacaacagg catgacggtg gcggagctgt ccgcggccgc cgtgcaatac agtgataacg
     1561 ccgccgccaa tttgttgctg aaggagttgg gcggcccggc cgggctgacg gccttcatgc
     1621 gctctatcgg cgataccacg ttccgtctgg accgctggga gctggagctg aactccgcca
     1681 tcccaggcga tgcgcgcgat acctcatcgc cgcgcgccgt gacggaaagc ttacaaaaac
     1741 tgacactggg ctctgcactg gctgcgccgc agcggcagca gtttgttgat tggctaaagg
     1801 gaaacacgac cggcaaccac cgcatccgcg cggcggtgcc ggcagactgg gcagtcggag
     1861 acaaaaccgg aacctgcgga gtgtatggca cggcaaatga ctatgccgtc gtctggccca
     1921 ctgggcgcgc acctattgtg ttggccgtct acacccgggc gcctaacaag gatgacaagt
     1981 acagcgaggc cgtcatcgcc gctgcggcta gactcgcgct cgagggattg ggcgtcaacg
     2041 ggcagtaagg ctctgaaaat catctattgg cccaccaccg ccgcccttgc gggcggcatg
     2101 gattaccaac cactgtcaca tttaggctag gagtctgcgc ggcagagccg tgtgaccggt
     2161 tttctgtaga gcactgacga tggcggcggc gctctctgca attggcaagg cgtcggcgcc
     2221 aaggatacca atcttgcggc gcgcggcgtg ttatgacgac tggggtgcat ttgagccgcc
     2281 ccatttaacc ttcgccctca cagatacgcc attcgcctca gatttagcgc catgcagacg
     2341 agcttccact cggcttgcac cttgtccagg cccctcatgc tgaactgacg caatcccatc
     2401 accgccttga tccaaccatt cggagcctcc acgatcgact tgcgccggcg gtaagctgca
     2461 tcgccttgct ccgttttcaa tttcgccgca atcgccgccg tatgcggatg ggtcttggca
     2521 ttgaccttgg catcttcacg tccctcgcgg ccgagggcaa cgatgacatc gccgtggtga
     2581 tcggcgacct ttgccagaac agcctcacta cggaatcccg catccgccag cgtctgggcc
     2641 ggcatttctc cggtgttggc ctgaactgct gccagcatgc ccagcagcgc ctgactgtcc
     2701 gcggcgcagt tggtcaactc cgccgccacg atgatctggt gctcggcatc gaccgctgtg
     2761 tacccgttgt agctctgctc ggagccacca ccggcgtgtt tcatgatccg gctgtccgga
     2821 tcggtgaagc tttcctgatc acggtcatcc ggcacaccaa actcgcgttt gtacgagcca
     2881 ccgcccttgt ccgagccatc cggatggcga ggccggcggc catcgtcttc gctgcgcccc
     2941 cgggcctggt ccgcttcacg ctggcgcgct tccaggcgcg cctttgccgc ctggatcgcc
     3001 tccaggcgct tctcgcggcg agaaatctcg gcaggaatgt ccagctccgg ctcgttacgc
     3061 tcctggtcgt cggtagcctt ggcgcgatca agcagcgcct tgatctcgca atgcaattcg
     3121 tcctcggccg gcttcatgcg cttatagctc atcgccttgt ggcggctggc gttggctttt
     3181 accttggtgc cgtcgaccgc gatcgtgcca agcttcacca gcccacattc gcgcgccagt
     3241 tgcaccacct gaacgaacaa attctcaagc tcggtcaggt gtagggcacg gaagtcactc
     3301 agcgtgcggt gggccgggaa gtttccagcg gccagcacac gcaacgcgac atcctcgtac
     3361 agcttcctgg ctagtttgcg cgaagagaag acgccgctcg cgtaaccata gatcagcact
     3421 ttgaccatca tcgccggatg aaacggctga ttgcccggac caccgccggc ataccgggcg
     3481 tggaatgcgc tcaagtccag cgtatcgaca gtctcgctga tgaagtaggc aagatgccct
     3541 tcaggtagcc actctcccag agaagggggc agcagatagg attggtcggg tcggtaagga
     3601 aggtaactgg cagccatccc cgtatcgtcc tcgatcttcc gccgatttgg cttctgccgc
     3661 gcaggctcct aggccaccgt agctaaacat acgagaaaaa ccgcttcttg agcgctctct
     3721 gagaccacct tgccgacaac gcagaaacgg ttgcggaaaa atcggcgttt ccggcgtacg
     3781 ccacccgacc tggacgaact attgcggacc tggaactgga gtagcggaat tccccggggt
     3841 ttcagcgtcg cgccacagcg cctcagatag atgcggtagc ctttggcggg tcgtcatgcc
     3901 gcggaccacc cccccaaaaa attccacaat gcggaatggc ggaattttct ggggttcccg
     3961 cttacccccc ctaatagatg ggtgctttca tgtttggttt ttcaccagga gaacttatga
     4021 acctttgccc cgatgaacgt ctgctctttg tgcggatgat ttccgctatg cttcgccgtt
     4081 cgggcggtga tgcgggtgct gttatgtttg aggcgtatcg ccatattgtg tccgatacca
     4141 atcaagctcg tcgttcctac atgctcgacc tgctcgaaag cgtcaggcat gactatgtac
     4201 acggtggata tacatagttg ccctggatac gcttttcgtc taagtgagcg ttcttttcgt
     4261 ctctttattc gccgtcctgt ataccgaagc gtgcgctcag cttctgataa agcgcttctg
     4321 cctgttcgtg cattcgctca cagtcggctg cctcgtcatc aagatcgagc acgtcgagtt
     4381 tttcgagcag cagcagcgac tgcgcccgga taaattttgc catgttcagt gcggctgatt
     4441 cctgttttgt catacggctt tttcccggtt ttatggtgat cgcagactga aatcttactt
     4501 cattcacttg agtgcatagc catgcaggta cgcagaaatc atcaggggcg ttttttctcg
     4561 atatgggcac agaaaggtgt atgtttcagg ccggagtcct cagaaacgcg ctgagggcgt
     4621 ctcaggcggt gcgggcaacg gatgttatgg taaatctgcc tcacagactg gtacaaaaaa
     4681 agccccgcag ggcttttttg ctatgagtta gccatatttt cttgcgaatt tttcatcgga
     4741 atcgcataga taaataataa actcaataaa cgcgatgatc gcgggtatga atgtccagca
     4801 aaaaattaga tacaggaatc cctgtcctat tttacctaag taaaatttat gtacgccaaa
     4861 tccaccaagg aagaatgcta atagcgcggc tgcgattcta cttttttcac ctgaacctga
     4921 agaggcgttt gtggctccgc agtgtgggca tgactttgct gtttcgtgta tttccttgcc
     4981 gcatcctcgg cagtaaacca ttgaactcat tttttatcct cgagcttatt tttagttggg
     5041 atgcataata tccgatgttt tataacgata tgcatcctat atgctcgggt gggatagatt
     5101 tccattatgg tgaaggatct tcttgagatc ctttctgtag cgtcgtaatc tcttgctctg
     5161 taaacgaaaa aaccgcccag caaggcggtt tttcgaaggt tctcagagca gcaactcttt
     5221 gaaccgtggt aactggctta cgaggagcgc aggaaccaaa tctgttcttt cagtttagcc
     5281 ttaacaggcg cataacttca agactaactc ctctaaatca gttaccagtg gctgctgcca
     5341 gtggcgcttt gtcgtgtctt tccgggttgg actcaagttg atagttaccg gaaaaggcgc
     5401 agcagtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgccttcc
     5461 cggaactgag tgtcaggcgt ggaatgagat aaacgcggcc ataacagcgg aatgacaccg
     5521 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc taccaggggg aaacgcctgg
     5581 gatctttata gtcctgtcgg gtttcgccgc cactgatttg agcgtcagat ttcgtgatgc
     5641 ttgtcagggg ggcggagcct atggaaaaac ggcttcgccg cggccttatc gtttccctgt
     5701 taagtttctt cctggcatct cccaggagat ttccgcccca ttcgtaagcc cgcaccgctc
     5761 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa tatctcctgt
     5821 ctcatatatt ccgcttacgc tctgctgctg cattttcctc ctgccacatg aagcacttca
     5881 ctgattcccg cacccgtgcc tacatagtaa gccagtatac actccgctag cgctacgtga
     5941 ctggttcagg gctgcgcccc gacacccgct aaaagcactg acgcgcctgc ggcttgccca
     6001 accccgcgcc gtccgggaaa gaatttcccg atcgacccga cgtttctgtt tacctgtgat
     6061 gttaacgttt ggatcttttt cccgggttta cctattttct tctaatgaag cgttcacagc
     6121 tgacgttaat aggggttcta agcgggaatc ccagaaaatt ccgtcattcc gtatgatgaa
     6181 aattcagtcg actcctcgct tgacgtgacg agcgccaaaa gcctgccgca tctgcctggc
     6241 gcgctcgagc tcatcgccat gcaaatacat cgacgtcgtg gcgatcgacg catggcgtag
     6301 gttgtcgcgc accgcggtga gctgagctcc ccgggccaac gcatggctgg catgactgtg
     6361 acgcatccaa tgaggcgtgg cacggcgcag cttttcggcg gcgaccggat gatcggcctc
     6421 aatggcctca gcggcttgcg taaagaatct tcgcaacacg caccaaaggt gagggccggt
     6481 gatgccggca ttaccgtctt ccccaagact gcccaccagg ggcgttttcg gattccaccg
     6541 ttcccggctg actggcagtt gacgctgtgc caagtattgg tccagcgccg tatacgcgag
     6601 cggcggcaag gccaccttac ctggtttgcc acctttgcca accaaatgca gccagtggtc
     6661 accgtgctcg tcgaggcgga cattacccag gacggcgccg accagttcgc tagcgcgcag
     6721 gcccgtggca tagccgaagt cgagcatgaa gcgcaggcgt tgtgctgccg gctcggacca
     6781 gccgtaggac cattccaggc cgtctgcaat ggcgcgcagc agtagccatt ctccctcggt
     6841 aaagcctcgc gccgtatcca gtgcgggccg caaggcgtga ccgcgtacct tgacgccggc
     6901 gaaagggttg gccagcacat agcgctgctc gatgagccac cggaacagcg ccgagagcac
     6961 cgtcagcgca tacgctgccg agcgtgcgga caagccgccc gagaaggggc gccattccac
     7021 cgaatcgcgt ggtcgaggtg gccccaccca acgctcgcgc ggggtcgggt gccggacaaa
     7081 ggcccggtac gcgattgcat cgtccgtggt cagggatgat agcgcccgtc cgcgttcgac
     7141 gatggcccac aggattagcc gcgcagcctc cttgcgatag gcgcgctgtg tggcgacagt
     7201 ctcgtgcagc gccagccagg cctgcacggc ctcgtagtcg ttggtcgcat tcagcgtgca
     7261 agcgttgcgc ggggcccgga aattgccctg cgagccgtcg acttcgttcg gcagacgcaa
     7321 ctgctcccac ggcacgatgc cgctgggctg aataatcggg atcagggccc gggcgcgctc
     7381 ggtgagctgc ggatgtgcgg caaagaaagc ctcgacctgc cgggcgcctg tcatgccgag
     7441 tccctcgatg gctttccacc agccacgccg gcggggaatg cgcacggtca gcgccgagag
     7501 ggtgtggata ccgtacgcct gcagtgccct gacggtcctc ggagacagcc actggtcgat
     7561 gctgtcgctg atctggggct tcggcgccgg cagcgtgggc aagagccgga tcgcgcgttc
     7621 gacggcaccc gcgcgcccct gtcgctcttc gggggcgtgc tggaacacgt cggccagatc
     7681 cggccggtgg cggctgttgg cgaaaacgat gagctggcgg cggatatgcc ccagcatccc
     7741 tcgcgctgat tgaccggcgg ccttgcgtgc cgccaggtag tgttcaacgg ccgcgcgaga
     7801 gctgagtccc gcgtaccagc cgcgcaaggc agccagcgaa tccgcatcgg gaaagccgac
     7861 tggggtagaa ctcgaagccg tagtctggcc cgttttcata tggacaggtt gcgattcgaa
     7921 aagcgagtaa cgcgataata acgattattt cagtagaatc gaaaatgccc catgtttcta
     7981 cgaaacctca agatagcaag aacgggccat gagcaagaag aacaagctac tcagcgtcct
     8041 atccgacgcc gaacaggagg ccttgtatgg cctgccggag ttcgatgatg cgcagcagct
     8101 ggaatatctg gctgtgacag aaaccgagtt ggcgcttgcc aacagccggc ccagtcttca
     8161 tgccaaggtc tattgcctct tgcagatcgg ctacttcaag gccaagcatg ccttcttcag
     8221 tttcgactgg gacgaagtcg aggacgattg cgctttcgtg ctgagccgct atttccacgg
     8281 cgaggtgttc gaacccaagg cgatcaccaa gcatgaacgc tacacccagc gcgagcagat
     8341 cgccaaacta ttcggctact ggccgtggtc ggccgccttc ctgccgcagc tcgagcagca
     8401 ggccgcacgg accgtgcggc gtgacgtaac gccggggttc gtcgccgccg agctgatcgt
     8461 ctggctcaat gagcacaaga ttatccggcc cggctatacg accttgcaag agttggtcag
     8521 cgaaacgcta tccgccgagc gccggcgcct gggcaatctg ctggagcaag cgttggacga
     8581 atctgccaag gccgcgctga gccagcttct ggtccgtgat gacaccctgt cgcaactggc
     8641 ggcgctcaag caggacgcca agaatttcgg ctggcgccag atggtccgcg agcgcgaaaa
     8701 gcgcgccacg ctggagccgc tgcacgcaat cgccaagaca ctgctgccta aactcgacgt
     8761 ctcgcagcag aacctgctgt actacgcgag cctggcaaac ttctacaccg tccacgacct
     8821 gcgcaatctg aaggccgatc agacccaact ctacctgctg tgctatgcct gggtacgcta
     8881 ccggcagctc tccgacaacc tggtcgatgc gatggcgtac cacatgaagc agttggagga
     8941 cgaaagcagc gctggtgcca aacaatcctt tgccgacgag caggtgcgcc gtcagcagga
     9001 aacgccgcgg gtaggccgcc tgctgtcact ctacgttgat gatagcgtgg ccgatcccac
     9061 gcccttcggc gaggtgcggc agcgcgccta caagatcatg cctcgcgata cgctgcaaac
     9121 cacggcgcag cgtatgagcg tgaagccggc gagcaagctg gccttgcagt ggcaggcggt
     9181 ggacgggctg gccgaccgca tgcgccgcca tcttcgtcca ctgtacgttg cgctcgacct
     9241 ctccggctcc aatccagaca gtccatggct tgccgcgctg gcctgggcca aaggtgtttt
     9301 tgccaagcag cagcggctgt cgcaacggcc actcgacgag tggcccgcgg cgacacttcc
     9361 gaaacgcttg cggccgtacc tgctgacctt cgacgccgac ggtaaaccaa ccggcctgca
     9421 cgctgaccgc tacgagttct ggctgtaccg ccagatcagg aagcgtctcc aatcgggtga
     9481 actctacctc gacgacagcc tgcaacaccg gcacttctca gatgaactgg ttgagatgga
     9541 caggaagatc gatgtgctcg caaaaatgga gatccccttc ctgcagcaac ccgtccatgc
     9601 ccaactcgat gcgctgactg ccgaactgcg cacgcagtgg ctggcgttca accgcgagct
     9661 gaaacagggc aagctgacgc acctcgaata caacaaggac acgcagaaac tgacctggcg
     9721 caagcccaag agcgagaatg agaaagcgcg cgaaaaggca ttctacgaga agcttccgtt
     9781 ctgcgacgtg gccgacgtgt ttcgcttcgt caacgacgag tgcaagtttc tgtcggcgtt
     9841 cacgcccatg cagccgcgct acgcgaagaa ggtcgccgac gctgacagcc tgatggcggt
     9901 catcatcgcg caagcgatga accacggcaa ccacgtcatg gcgcgcacca gcgacatccc
     9961 gtaccacgtg ctggatagcg gctaccagca gtacctgcgc caggcaacgc tgcacgcggc
    10021 caacgactgc atcagcaacg ccatcgccac gctaccgatc ttcccatact actcgttcga
    10081 cctcgaaacg ctgtacggtg ccgtcgacgg gcagaaattc agcgtcgaac ggccgaccgt
    10141 gaaggcgcgc tattcgcgca agtacttcgg gcgcggcaag ggcgtggtcg cctacacgct
    10201 gctgtgcaac cacatcccgc tcaacggcta cctgatcggc gcgcacgact acgaggccca
    10261 ccacgtgttc gacatctggt atcgcaacac gtcggacatc atgccgaccg cgatcaccgg
    10321 cgacatgcac agcgtcaaca aggccaactt cgccatcctg cattggtttg gccggcggtt
    10381 cgagccgcgc ttcaccgacc tcaacgcgca attgaaagaa ctctactgcg ctgacgatcc
    10441 agcacagtat caggcgtgcc tgattcggcc agtcgggaaa atcgactgcg atctcatcca
    10501 tcgcgagaag ccgaacatcg accggatcgt cgccacactc gggctgaagg aaatgacgca
    10561 gggcacgctg atccgcaagc tgtgcaccta caccacgacg aacccgacgc ggcgcgcgat
    10621 cttcgagttc gacaagctca tccgcagcat ctacacgctt cgctacctgc gcgatccgca
    10681 actggaacgc aacgtgcacc gctcgcaaaa tcgcctcgag tcctaccatc agctacgctc
    10741 ggccattgcc caggtcggcg gtaagaagga actgaccggt cgcaccgaca ttgaaatcga
    10801 gatcagcaac cagtgcgcac ggctgatcgc caacacgatc attttctaca actcggcaat
    10861 cttgtcgcgg ctggtgacca agtacgaggc ggccggcaac agcaaggcgc tggcactcat
    10921 cacaaaaatt tcgcctgcgg catggcggca catcctgctc aacgggcact acacctttca
    10981 aagcagcggc aagacgatcg atctcgatgc gatcgtcgca ggcctggaac tggagtgacg
    11041 gaattttcag cggttccgtg ttagcagcag tgtaaaactg acccacccca gcgaagtaaa
    11101 actgatccac ctgggctagt ttggcggcct tttcgaggcc gtcgatgttg acccaggagc
    11161 aaaccgtgga aatcagagtt cttgcccgac agggccactc catccggcac atcgcccgca
    11221 cgttgggggt ctcgcgcaat accgtgcgcc gctacctccg cgatccgtcg gtagcgcgat
    11281 accagccccg tgaaccacgc cccaccaaac tggggccatt tgaatcttat ctgcggcaac
    11341 gggtcgagca ggcccacccc atctggctgc ctgccaccgt cctgaatcgg gaaatccgcg
    11401 ctcaagggta tggcggcggt ctctcgttat tgcgcgcctt cctcgccacg ctcaagcccg
    11461 cccgccgcga agcaggtccg gccgtccgtt tcgagaccga gcccggacgg cagttgcagg
    11521 ctgatttcgt ggtcttgcgc cgggccagat cccccatgtc ggccttcgtc gccacgctgg
    11581 gatacagccg catgacgttt gtcaccttcg taccggacga atccttcgag tccgtgcggg
    11641 actccctgtt gctggctttt gactatctgg gcggtgtgcc ccgcgaggtg ttgttcgaca
    11701 acatgaagac cgtggtgctg gaacgcgatg cctacggtga cggcaagcac cgttttcacc
    11761 ccggcttgct tcaactggcc gatgacctgg ggttccggat ccggctgtgt cgcccgtatc
    11821 gggcccagac caaaggcaag gtcgagcgct tcaatcgcta cttccgcgag agcttctaca
    11881 acccgttgct gacccggatg aagggcacgg ggctgctgct cgattgtgct gctgccaacc
    11941 gacgcgtccg tgactggttg gcagacgagg cgaatgtgcg ggtccacgcc accctgaacg
    12001 agcgccccat cgaccgttgg cggcaggagc gggaacacct gcaacccttg ccatcccgtg
    12061 tgcggcgcga cgaggccccc ctgctggata acagtctccg cccggtacca ctggaatctc
    12121 tccagcaccc actgtctgtc tatgacgcaa ttggggaggc ctgccg
//
