LOCUS       NZ_CP118228             8441 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Proteus mirabilis strain CY32 plasmid pCY32-1, complete sequence.
ACCESSION   NZ_CP118228
VERSION     NZ_CP118228.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33299137
            Assembly: GCF_028768505.1
KEYWORDS    RefSeq.
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Pan,y. and Yu,l.
  TITLE     Characterization of a blaCMY-2-carrying Proteus mirabilis isolate
            with coexistence of a genomic resistance island PmGRI1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   Pan,y. and Yu,l.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2023) College of Animal Science and Veterinary
            Medicine, Henan Agricultural University, Wenhua Road No. 95,
            Zhengzhou, Henan 450002, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycle v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 498.3x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028768505.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 22:39:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,780
            Genes (coding)                    :: 3,713
            CDSs (with protein)               :: 3,713
            Genes (RNA)                       :: 110
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 18 of 67
            Pseudo Genes (incomplete)         :: 51 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118228.1.
            Bacteria and source DNA available from Yushan Pan.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="CY32"
                     /host="chicken"
                     /db_xref="taxon:584"
                     /plasmid="pCY32-1"
                     /geo_loc_name="China:changchun"
                     /collection_date="2009-10-30"
                     /collected_by="Yushan Pan"
     gene            1..840
                     /locus_tag="PU712_RS19390"
                     /old_locus_tag="PU712_19390"
     CDS             1..840
                     /locus_tag="PU712_RS19390"
                     /old_locus_tag="PU712_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_010904471.1"
                     /translation="MATHKPIDILASFTELPPPIDYVLPNMVAGTVGALVSPGGAGKSM
                     LVLQLAAQIAGGPDLLDVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLTDEQRQVVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSGMTAAEAEE
                     WGVDDSQRPYFVRFGVSKANYGAPFKDRWFRRHDGGVLKPAVLEKQRKSKGVRRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="PU712_RS19395"
                     /old_locus_tag="PU712_19395"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="PU712_RS19395"
                     /old_locus_tag="PU712_19395"
                     /function="putative replication protein"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_010895881.1"
                     /translation="MVKPKNKYSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFKGPEPLGADDLRILQGLVAMAGPNGLVLSPEPKTEGGQQLRLFLEPKWEAV
                     TADAMVVKGSYRALAREIGYAEDGGSQFKAIRECIERLWTVSIIAQNGRKRQGFRLLAE
                     YASDEAGGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSEAARLLHQRLCGWIDPGK
                     TGKAAIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            <1727..2182
                     /locus_tag="PU712_RS19400"
                     /old_locus_tag="PU712_19400"
                     /pseudo=""
     CDS             <1727..2182
                     /locus_tag="PU712_RS19400"
                     /old_locus_tag="PU712_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            2213..3097
                     /locus_tag="PU712_RS19405"
                     /old_locus_tag="PU712_19405"
     CDS             2213..3097
                     /locus_tag="PU712_RS19405"
                     /old_locus_tag="PU712_19405"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="WP_001447541.1"
                     /translation="MLRSQRRGRDPPCWLSEKEPLVGKRRVNFQRCWLPVSRIGLHRRG
                     VERVNCGPKLLDLGEKGVYSSYTCRVPGGDYEGYVDAHVRRLEALRRAGIVERIDADQW
                     RIPDDLVSRAAAHDAGRDSQASVRVLSPVDLNKQIGSDGATWLDRRLIHGETADLAPTG
                     FGQQVREAMDQRREHHIEQGDATRSRDSRVFYRRNLLAILREREVAGVGSDMALSKGLP
                     FRAATDGESVSGKFTGTVHLSSGKFAVVEKSHEFTLVPWRPIIDRQLGREVMGIVQGGS
                     VSWQLGRQRGLER"
                     /name="DUF3363 domain-containing protein"
     gene            3314..4528
                     /gene="floR"
                     /locus_tag="PU712_RS19410"
                     /old_locus_tag="PU712_19410"
     ARG             3315..4525
                     /name="floR"
                     /gene="floR"
                     /gene_name="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4556..>4675
                     /locus_tag="PU712_RS19415"
                     /old_locus_tag="PU712_19415"
                     /pseudo=""
     CDS             4556..>4675
                     /locus_tag="PU712_RS19415"
                     /old_locus_tag="PU712_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /name="LysR family transcriptional regulator"
     gene            complement(4630..4980)
                     /locus_tag="PU712_RS19420"
                     /old_locus_tag="PU712_19420"
     CDS             complement(4630..4980)
                     /locus_tag="PU712_RS19420"
                     /old_locus_tag="PU712_19420"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_223424540.1"
                     /translation="MAFAIMRAKKLNSMGTVAAALQHCYRDRETPNADQERTPDNDHLA
                     ARSTDEAMGKLRERLPEKRRKDAVLAVEYVMSASPEWWQTASADQQREFFKRSTEWLAA
                     CRKFRCSATAMN"
                     /name="plasmid recombination protein"
     gene            complement(5480..5779)
                     /locus_tag="PU712_RS19425"
                     /old_locus_tag="PU712_19425"
     CDS             complement(5480..5779)
                     /locus_tag="PU712_RS19425"
                     /old_locus_tag="PU712_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010904468.1"
                     /translation="MAKTAERLAKLEEQRARINAEIQRIRAREQQQKRKENTRRKVLVG
                     AWILGKVENGEWPEQRLLDGLDSYLERDHDRALFGLPPKQKQQPQEHGQPEPQG"
                     /name="hypothetical protein"
     gene            5961..>6602
                     /locus_tag="PU712_RS19430"
                     /old_locus_tag="PU712_19430"
                     /pseudo=""
     CDS             5961..>6602
                     /locus_tag="PU712_RS19430"
                     /old_locus_tag="PU712_19430"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            6709..7119
                     /locus_tag="PU712_RS19435"
                     /old_locus_tag="PU712_19435"
     CDS             6709..7119
                     /locus_tag="PU712_RS19435"
                     /old_locus_tag="PU712_19435"
                     /function="putative role in conjugative DNA replication"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010895877.1"
                     /translation="MSAIDRVRKSRGINELAAEIEPLAQSMATLADEARQRIAEVQQAS
                     EEQAASWTSQQQQAMSAWRQAAKDMRAAAGELAKAGQTARSAARGWTWRLWAGVLIASV
                     MPILALLIASWLWLEPQIIEQQGSIWLIFKLK"
                     /name="IncQ-type mobilization protein MobB"
     gene            <7218..8072
                     /locus_tag="PU712_RS19440"
                     /old_locus_tag="PU712_19440"
                     /pseudo=""
     CDS             <7218..8072
                     /locus_tag="PU712_RS19440"
                     /old_locus_tag="PU712_19440"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /name="DNA-primase RepB domain-containing protein"
     gene            8140..8376
                     /locus_tag="PU712_RS19445"
                     /old_locus_tag="PU712_19445"
     CDS             8140..8376
                     /locus_tag="PU712_RS19445"
                     /old_locus_tag="PU712_19445"
                     /function="putative role in regulation of gene expression"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010895879.1"
                     /translation="MDIGKAKEYFGLGLIQGAVVRSSSVLHNGWTIELSGNIGSAQPTL
                     HTARGDVRQFKTLDAAAKVVREIGLREWRVITD"
                     /name="hypothetical protein"
     Typing          5812..5899
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.81"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5962..8069
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.573"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctactc ataagcctat cgatattctg gcgagcttca cagagttacc gccgccgatt
       61 gactacgtac tgcccaacat ggtggccggt accgtggggg cgctggtgtc ccccggtggg
      121 gccggcaagt ccatgctggt tctacagctg gccgcacaga tcgcaggcgg ccccgacctg
      181 ctggacgtgg gagagctgcc caccggcccg gtgatctact tgcccgccga agacccgccc
      241 accgctatac atcaccgcct gcacgcattg ggggcgcacc tgaccgacga gcaacggcaa
      301 gtcgtggctg acggtctgct aatccagccg ctgatcggca gcctgcccaa tatcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgttggat
      421 acgctgcgtc ggttccatat cgaggaagag aacgccagtg gccccatggc gcaggtgatt
      481 ggccgcatgg aggccatcgc cgccgatacc gggtgttcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggatcgtcc
      601 gtgctggtgg ataacatccg ctggcagtcc tacctgtccg gcatgacggc cgccgaggcc
      661 gaggaatggg gcgtggacga ctcacagcgg ccctatttcg tccgctttgg tgtcagcaag
      721 gccaactatg gggcgccgtt caaagaccgc tggttcaggc ggcatgacgg cggggtgctc
      781 aaacccgccg tgctggaaaa gcagcgcaaa agcaaggggg tgcgccgtgg tgaagcctaa
      841 gaacaagtac agtctcagcc acgtccggca cgacccggcg cactgtctgg ctcccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcaaggg gccggagccg ctgggtgctg atgatctgcg
     1021 catcctgcaa gggctggtgg caatggctgg gcctaatggc ctagtgctta gcccagaacc
     1081 caagaccgaa ggcgggcagc agctccggtt gttcttggaa cccaagtggg aggccgttac
     1141 cgctgatgcc atggtggtta agggcagcta tcgggcgctg gcccgtgaaa tcgggtatgc
     1201 agaggatggc ggcagccagt tcaaggcaat ccgggagtgc attgagcgcc tgtggacagt
     1261 ctccatcatc gcccaaaatg gccgcaagcg gcaggggttc cggctgcttg ccgagtacgc
     1321 cagcgacgag gcaggcgggc gtctgtacgt ggccctgaac cccttaattg ctcaggccgt
     1381 catgggtggt ggccagcatg tgcgcatcag catggacgag gtgcgagcgc tggacagcga
     1441 agcggcccgc ctgctgcatc agcgcctgtg tggctggatc gaccccggca agaccggcaa
     1501 ggcggccata gataccctgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggttgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccctgtttaa cagtcacgcc cctccccctc cgcactgtca gattgctacc agctcaagac
     1741 gccgtaccgg gacggcacca cgcacgtcat tttcgaacca ttggatttca ttgcaaggct
     1801 ggccgccctg gtaccgaagc ccagagtcaa cctaacccgc ttccacgggg tgttcgcacc
     1861 caacagtcgg caccgggcgt tggtcacgcc ggcaaaacgg ggcaggggca acaaggtcag
     1921 ggtggctgat gaaccggcaa caccagcaca acggcgagcg tcgatgacat gggcgcaacg
     1981 gctcaagcgt gttttcaata tcgacatcga gacctgcagc ggctgcggcg gcgccatgaa
     2041 agtcatcgcc tgcattgaag accctatagt gatcaagcag atccttgatc acctgaagca
     2101 caaagccgaa accagcggga ccagggcgtt acccgaaagc cgggcgccac cggctgagct
     2161 gctcctgggt ctgtttgact gacgagcctg aaggccaacg ataccaatca aaatgctgcg
     2221 ttcacagcgc cgcggcaggg atccgccgtg ctggttgtcg gaaaaggagc cgctagtggg
     2281 aaagaggagg gtaaattttc agcgttgctg gctccccgtc agccggattg ggttgcatcg
     2341 caggggtgtc gaaagagtca actgcggtcc aaagctgttg gacttgggtg aaaagggcgt
     2401 ttattcttcc tatacgtgcc gcgttccagg cggcgactat gagggctatg tcgatgccca
     2461 tgtgcgccgg ctggaggcgc tacgccgggc cggtatcgtc gagcggatcg acgccgacca
     2521 atggcgcatc cccgatgatc tggtcagccg tgccgccgcc catgacgccg gccgagacag
     2581 tcaggccagc gttcgcgtcc tttccccggt cgatctgaac aaacagatcg gatcggacgg
     2641 cgcgacctgg ctggaccggc ggctgatcca cggcgagacg gccgaccttg cgccaaccgg
     2701 cttcgggcaa caagtccgcg aagccatgga ccagcgccgc gagcaccata tcgaacaggg
     2761 cgacgccacc cgcagccggg acagccgcgt cttctaccgg cgcaaccttc tcgccatcct
     2821 gcgggagcgc gaggtagccg gcgtcggatc ggatatggct ttgagtaagg gcctgccgtt
     2881 ccgcgccgcc acggacggcg agagcgtcag cggcaagttt accggaaccg tgcatctatc
     2941 gagcggcaag ttcgccgtgg tcgagaaatc ccatgagttc acccttgtcc cgtggcggcc
     3001 gatcatcgac cgccaactcg gccgcgaggt tatgggcatc gtgcagggcg ggtcggtgtc
     3061 gtggcagtta gggcggcaga gggggctgga acgctgagtg cgcccatgcc gcattgcgaa
     3121 gcaaaagata atcggataaa atgtagcaat tcatattcgt aagcgtggag taatcagatg
     3181 ggaaattcca agtcagcaga caagtaagcc gcaacaacca gtattgttgt tgcggcgctc
     3241 tgtaaggcta gtctcatctg attgctgacg agcagacgtc gcccggtatt ccttaatcga
     3301 gggttgattc gtcatgacca ccacacgccc cgcgtgggcc tatacgctgc cggcagcact
     3361 gctgctgatg gctcctttcg acatcctcgc ttcactggcg atggatattt atctccctgt
     3421 cgttccagcg atgcccggca tcctgaacac gacgcccgct atgatccaac tcacgttgag
     3481 cctctatatg gtgatgctcg gcgtgggcca ggtgattttt ggtccgctct cagacagaat
     3541 cgggcgacgg ccaattctac ttgcgggcgc aacggctttc gtcattgcgt ctctgggagc
     3601 agcttggtct tcaactgcac cggcctttgt cgctttccgt ctacttcaag cagtgggcgc
     3661 gtcggccatg ctggtggcga cgttcgcgac ggttcgcgac gtttatgcca accgtcctga
     3721 gggtgtcgtc atctacggcc ttttcagttc gatgctggcg ttcgtgcctg cgctcggccc
     3781 tatcgccgga gtattgatcg gcgagttctt gggatggcag gcgatattca ttactttggc
     3841 tatactggcg atgctcgcac tcctaaatgc gggtttcagg tggcacgaaa cccgccctct
     3901 ggatcaagtc aagacgcgcc gatctgtctt gccgatcttc gcgagtccgg ctttttgggt
     3961 ttacactgtc ggctttagcg ccggtatggg cacctacttc gtcttcttct cgacggctcc
     4021 ccgtgtgctc ataggccaag cggaatattc cgagatcgga ttcagctttg ccttcgccac
     4081 tgtcgcgctt gtaatgatcg tgacaacccg tttcgcgaag tcctttgtcg ccagatgggg
     4141 catcgcagga tgcgtggcgc gtgggatggc gttgcttgtt tgcggagcgg tcctgttggg
     4201 gatcggcgaa ctttacggct cgccgtcatt cctcaccttc atcctaccga tgtgggttgt
     4261 cgcggtcggt attgtcttca cggtgtccgt taccgcgaac ggcgctttgg cagagttcga
     4321 cgacatcgcg ggatcagcgg tcgcgttcta cttctgcgtt caaagcctga tagtcagcat
     4381 tgtcgggaca ttggcggtgg cacttttaaa cggtgacaca gcgtggcccg tgatctgtta
     4441 cgccacggcg atggcggtac tggtttcgtt ggggctggtg ctccttcggc tccgtggggc
     4501 tgccaccgag aagtcgccag tcgtctaacc gacgactggt agcaggcccg ctccgatgcg
     4561 gcgcactaac catcgaaacc tcgtgaatgt cggtatcctg tctggcagga taccgctcat
     4621 ttcccttgtt cagttcatcg ccgtcgccga gcatctgaat tttcggcatg cggccagcca
     4681 ctcggtcgat cgcttgaaga actcccgctg ctggtcggcg cttgcggtct gccaccactc
     4741 cggcgaggcg ctcatcacgt attcgaccgc cagcaccgca tccttgcgcc gcttctccgg
     4801 caaccgctcc ctgagcttgc ccatcgcctc atcggtcgat ctggccgcca ggtgatcgtt
     4861 gtccggtgtg cgctcctgat cggcgttcgg cgtctcgcgg tcgcggtagc agtgctgcaa
     4921 cgcagccgcc accgtgccca tgctgttgag cttcttcgcc ctcatgatcg caaacgccat
     4981 ccgtcccccg gcgcaactgt cacgcccccg gcgcaactgt cacgcccccg gcgcaactgt
     5041 cacgcccccg gcgtaactgt cacgcccccc cggcgtaact gtcacgaacc cccggcgtaa
     5101 ctgtcacgcc cctaaacctg caaacccagg aaggggggga gctggtgggg tgttggaaaa
     5161 atccatccat gattatccaa gaataatcca ctaggcgcgg ttaacagcgc cctagtgggg
     5221 cgctgtttgc ccttgcttaa aatgccggcc agaggccgga taacttctct atccgctgcg
     5281 ctaggctaca caccgccccc acccgctgcg cggcaggggg aaaggcgggc aaagcccgct
     5341 aaaccccata ccaaatcccg cgggaatacg ctggagcgat tttagccgct ttagcggcct
     5401 tttccctacc cgaagggtgg gggcggtgtg cagccccgaa ggggccattc aggggccatt
     5461 ctgaccgctt tgcgcttggc tatccttgcg gctccggctg accatgctcc tgtggctgct
     5521 gtttctgctt gggtggaagt ccgaacagtg cccgatcatg gtcgcgctct aggtagctgt
     5581 ccaacccatc gagcaggcgt tgttctggcc actcgccgtt ttcaactttg ccgagtatcc
     5641 atgcacctac cagcactttg cgccgggtgt tttccttgcg cttttgctgc tgctcccgtg
     5701 ctctgatccg ttggatctcg gcgttgattc gtgcccgttg ttcttccagc ttggcgagcc
     5761 gttccgctgt ttttgccatt ttgataatcc tcttggttat gtgcttgtca ttatgctatc
     5821 atcggagcac agacaccgca aaccccgacc gtcattttac gggagggcgc acttaccggt
     5881 ttctcttcaa gaaactgccc tgggggcacc ccttcggggc tgtgcgctct ccgagggcca
     5941 ttgcatggag ctaaacccct atggccatct atcaccttac ggccaagacc ggcagccgga
     6001 acggcggcca atcggccaag gcgaaagccg attacatcca gcgggagggc cgttactccc
     6061 gcgaccgtga agaggtgctg cacacccagt ccggccacct gcccgagtgg gccgagcgtc
     6121 ccgccgacta ctgggacggt gccgacctgt acgagcgggc caacggtcgc ttattcaaag
     6181 agattgagtt tgccctgccg gtcgaactga ccctagatca gcagcgggag ctggtggacg
     6241 aattcgcccg gcatctgact gagggcgagc gcctgccgta tacgctggcc atccatgccg
     6301 gtaacggtga gaacccccat tgccacttga tgatctccga gcggaagaac gacggtatcg
     6361 agcgcccggc tgaccagtgg tttaagcggt acaacggcaa acagccagag cggggcgggg
     6421 cgcagaagtc cgagagcctg aagcccaagg catggcttga gcagacccgc gaggcatgga
     6481 gccactacgc caaccgcgcc cttgaacagg ccgggcacga ggcccgcatt gaccatcgca
     6541 cccttgaggc gcagggcatt gagcgcttgc cgggcatcca tcttggccct aacgtggtgg
     6601 agatggagag ccggggcatc cgcaccgaga gggccgatat cgccctagcc atcgacacgg
     6661 ccaacggcca gatcatcgac ttacaagaat accgggaggt tatcgagcat gagcgcgata
     6721 gacagagtga ggaaatccag agggatcaac gagttagcgg cagagataga gccgctggcc
     6781 cagagcatgg cgacactggc ggacgaagcc cggcagcgga tcgccgaggt acagcaggcc
     6841 agcgaggagc aggccgcgag ctggacgagc cagcaacagc aagccatgag cgcatggcgg
     6901 caggcagcaa aggacatgag ggcagccgca ggggaactgg ccaaggccgg ccagacggcc
     6961 cggagcgccg cccgtggctg gacatggagg ttgtgggccg gggtcttgat cgcttccgtg
     7021 atgcctattc tggcgctgct gatcgcatca tggctttggc tggagccgca aatcatcgag
     7081 caacagggga gcatatggct gattttcaag ctcaagtgaa aggtgaccgc accgcgcaag
     7141 ccatcgcccg gcagctcaag gccatgggct gcgaccggta cgacatcggc atacgggatg
     7201 ccgccagcgg caagatgatg aaccgggaat ggacaccgca ggaagtgcag cagaacgccg
     7261 cctggctcaa gcgcatgaat gcccagggca acgatattta cattcgcccc gccgagcagg
     7321 cccggcatgg tctggtgcta gttgacgacc tcagcagcga cgatctggac gccatgaagc
     7381 aggagggccg ggagcctgcc gccatcatcg agaccagccc caagaattat caggcatggg
     7441 tgaaggtggc tcaggatgcc ccagcagatc ataggggcgt aatcgcccgg aagctggccc
     7501 gggagtatga cgccgacccg gcaagcgccg acagccgtca ctatggccgt ctggcaggct
     7561 ttaccaaccg caaggacaag tacaccagcc gcaccggcta tcagccgtgg gtgctgtgcc
     7621 gggagtccag cggcaagagt gccaccgcag ggccggagct gatgcagcag gccgggcagg
     7681 tgttggacag catcgagcgc cgacaggaac ggacggcacg actggccgag atcaccgccc
     7741 cgcagagtgt gcgtcggtat cgccgcagcg tcgtggacga ctaccgcagc gaaatggccg
     7801 ggctggtcaa gcggtacggt gatgacctca gcaagtgcga cttcatcgcg gccatgaagc
     7861 tggccagcaa gggccgggag ccggacgaga tcagcaaggc catggccgag gctagccccg
     7921 ctatcatgga gcgcaaggcg ggccatgagg ccgattacat caagcgtacg gtgcagaagg
     7981 tgatggagct tccgcaggtg caggaggccc gcgccgagct ggcgaagcag acccagaagc
     8041 agcgcagcaa ggggccagat ttaagcatgt aatgcttgtt ttgataaaac aaaactgcta
     8101 tattgtcaaa acaatttgac tttatagtag gattaaaaaa tggatatagg aaaggcaaaa
     8161 gagtattttg ggcttggatt gattcagggt gccgtggtga gatcgtcaag tgttctgcat
     8221 aacgggtgga ctatcgagct gagcggcaac attggcagcg cacaacctac cttacacacc
     8281 gccagaggtg atgtgcggca gttcaagaca ctggatgcgg cggccaaggt cgtgcgagaa
     8341 attgggctgc gagagtggcg tgttatcact gactaggcgg cgcatggcgg ggatgccccg
     8401 ccagcgcctg accatcaacc gcctgcaaag gaggcaaacc a
//
