LOCUS       NZ_CP118193             3674 bp    DNA     circular CON 27-MAR-2024
DEFINITION  Klebsiella grimontii strain 2020CK-00211 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP118193
VERSION     NZ_CP118193.1
DBLINK      BioSample: SAMN15907146
            Assembly: GCF_028748965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00211
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028748965.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 02:05:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,171
            CDSs (total)                      :: 6,050
            Genes (coding)                    :: 5,863
            CDSs (with protein)               :: 5,863
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 64 of 187
            Pseudo Genes (incomplete)         :: 132 of 187
            Pseudo Genes (internal stop)      :: 31 of 187
            Pseudo Genes (multiple problems)  :: 37 of 187
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP118193.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00211"
                     /isolation_source="Tracheal Aspirate/Wash"
                     /host="Homo sapiens"
                     /db_xref="taxon:2058152"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2019-09-04"
     gene            complement(294..575)
                     /locus_tag="PU992_RS30760"
                     /old_locus_tag="PU992_30760"
     CDS             complement(294..575)
                     /locus_tag="PU992_RS30760"
                     /old_locus_tag="PU992_30760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032440460.1"
                     /translation="MIKNIKHKGLRSFAEEGSTAGIQPSMAKRLRMILTTLSTATCIQD
                     VVDVRALGCHPLKGERKGEYSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            913..1206
                     /locus_tag="PU992_RS30765"
                     /old_locus_tag="PU992_30765"
     CDS             913..1206
                     /locus_tag="PU992_RS30765"
                     /old_locus_tag="PU992_30765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609279.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023317864.1"
                     /translation="MINLYDPVHPGEFIREMFMEPFEIPAAELADKLDVSQSTLSRLLN
                     GKTDLSYEMALRLSKVVGRSAESWVNMQTQFSLSKSRKSLAAELEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            1270..1572
                     /locus_tag="PU992_RS30770"
                     /old_locus_tag="PU992_30770"
     CDS             1270..1572
                     /locus_tag="PU992_RS30770"
                     /old_locus_tag="PU992_30770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="WP_023317863.1"
                     /translation="MVDFYISAIKMDSAGQHIEFVKVHKINRDGSWNTAGSISSRKFIS
                     ELISMGKASFMTVVKNSSTGKWNLGAKVEAMNDGYITTDPNKTKLDNLGNLPKFV"
                     /name="DUF3892 domain-containing protein"
     gene            complement(2356..2757)
                     /locus_tag="PU992_RS30775"
                     /old_locus_tag="PU992_30775"
     CDS             complement(2356..2757)
                     /locus_tag="PU992_RS30775"
                     /old_locus_tag="PU992_30775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_023317862.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKEGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQGEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2757..2948)
                     /locus_tag="PU992_RS30780"
                     /old_locus_tag="PU992_30780"
     CDS             complement(2757..2948)
                     /locus_tag="PU992_RS30780"
                     /old_locus_tag="PU992_30780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023317861.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGY"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3364..3510
                     /locus_tag="PU992_RS30785"
                     /old_locus_tag="PU992_30785"
     CDS             3364..3510
                     /locus_tag="PU992_RS30785"
                     /old_locus_tag="PU992_30785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_073545459.1"
                     /translation="MHEPPVQSDCCTLSGNYHLESNPVRHAQAPLAAATGKKVYKRLLI
                     QSS"
                     /name="replication initiation protein"
     Typing          3070..3473
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.309"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcaaagcagt caaggaagaa ccaagacagc tcactcacca gcaggctcct ttttattggt
       61 agacacatgc ttctggacag tatcagcccc tgcacaaagt acagcagcta acaagaacca
      121 tccccagcca tcaataccac gaaaagctag gactccagca atagttacgc taatcaacgg
      181 gaacaataaa accataaagg atctcatact ttctccagag gttacttttt taataaagtc
      241 cctaatccat ccgtactttc acttggatgg attagcaact tacttattga taactaatga
      301 taatcttcaa gatcaacgtc taagacgttt ggttcctcaa ccttgaaaac tacccgccag
      361 ttcccattaa cttcaacgga atactcgcct ttacgctccc cctttagggg gtggcaccct
      421 aacgccctaa cgtcaacaac gtcttgaatg caagttgctg tgcttagcgt agtcaaaatc
      481 atgcgaagtc ttttcgccat actgggctga ataccggcgg tgctaccttc ttcagcaaag
      541 ctcctaagtc ctttatgttt tatgtttttt atcatctaca atccccatat ttttttcgtc
      601 tatacctgta tctatatttt aaaatcattt taattagttc cattttgccc ccttgcttta
      661 ttaaaacaat tgacgtaagt ttgttcttga ggtcaatcac ctcatttgac ttcaacgaat
      721 cctaagggta aaatactgcg ccttgaaaag gccataaaag cccatacata cgcagtagct
      781 ttgactccaa cgagtatcct aaagctgact gctttttttg tcaatatcat gaacttgcac
      841 attgtgcaat gcgcatactg aaaggcagta attttttttg tataatcaaa tacaaaacaa
      901 acagaggaag gcatgataaa tctttacgat cctgttcatc ctggtgagtt tatcagagaa
      961 atgttcatgg aaccgttcga aatacctgcc gcagagttag ccgataaact tgatgtatcc
     1021 cagtctacct taagtagact gttaaatggg aaaacagact taagctatga aatggcactt
     1081 agattgtcga aggtcgtagg ccgctctgca gagtcgtggg taaacatgca gacccaattc
     1141 agcctgtcaa aaagtcgcaa atctttagcg gcagagctgg aacacttgca tccaatgttt
     1201 ccatgaaaaa agcccgcaga acgcgggctt ttgttacgta aaacacccag cagccatcag
     1261 gaggcaacca tggtagattt ctatatctca gcaatcaaaa tggacagtgc tggtcagcac
     1321 atcgaattcg taaaagttca taaaataaac cgagatggct catggaacac tgccggctca
     1381 atcagctctc gcaaattcat cagcgagctg atcagcatgg gaaaagccag ctttatgaca
     1441 gttgtaaaga acagctctac tggtaagtgg aacctaggag ccaaagtgga agcgatgaac
     1501 gatggctaca taaccacaga tccaaacaaa actaaactcg ataacttggg aaatctgccg
     1561 aaatttgtct gatatgcaat aacatccact aagttccagc agaatcccct ccctaatgat
     1621 ttaacataaa gtcataattg tacgcaccgc taaaacggcc tcatggcgtt tctgacggga
     1681 aaccggtaaa acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt
     1741 ttacggcatg catgagtatg cacgcaagtg catagcgcga aaattcacgc cattcagcca
     1801 gggtaagtcc tgtttccggc gttctttcca tccgaaaacg gtgaacgggg acgcaattta
     1861 ttgcgtggtc gtgagccgtt gccggcgcag ccggaaaacc cgttttcagc acgttcagga
     1921 atcgggttcg tcgacggccg gcaatcacgt tatggcaagt cgccggacga ctcaccacac
     1981 gattcaccgg cgattcgact cgtgaattga atcaagagtc accatgaaca aaaaacaccc
     2041 aaagtgatga attgaatcaa gagtcaattc atcataacca tatgataata aagcgtgatt
     2101 cacatatgaa tcgtgggcaa ttcgtcagcg attcgccata gagtcgccca cgactcggat
     2161 tcaccttaca aaaaataaaa ttgcgtaatt tacaatagat aactaattga aactaaaggt
     2221 ctatctgcaa aattttaatt ttgcgtgggg tgtgggtatt tttcgtggtg aggagtgctc
     2281 agagatctgg cgatttttgg gaaggttcag gaactccccg gaatgaccgg agaggaaaac
     2341 tgaagcatga cgcggtcagg attttttcca gaatttccac catggcgccg gggattcagt
     2401 taattttgcc tggtgatttt caagaaggag catggcctgt ttcaggctgt caatgtgcga
     2461 atccttggac aaaagtaagg tattcaggtg ttcaatctcc tgtttcagat gacgaatcac
     2521 ctgttcaccc tggtcagcag aggctgtatc acgctgttca gagacctgtc cagaatggtg
     2581 ttcacccttc ggttgctcaa agggaccaaa gacacgtaaa agctctgaag tatctaccat
     2641 ttttgcatta cgctcaccgc tacaggtgga caggtcacct tctttaatta acctgtgcag
     2701 tgttgttcta ctcttacccg tcagtcgggc agcctcacta atagacactc tggccatcag
     2761 taaccatcct gtaatgcatc cagtctcgag gacagtgtac ggaaaagatg ctccgcatca
     2821 tcgtggagtt tctcacaaag gtcggcctcg acatcgagat caagctcgtt aagcttctcc
     2881 agcaggagca gagactgatt ctggatgaat ttagccatgt tcagcgctgt tttctgttgt
     2941 ttcgtcataa gaggcctcag agcgtcagtg ctcagtgtag ggatgttctc ttttccgctt
     3001 cctcgctcac tcagtcgcta tgctcctgcc gttcggctgc ggcgagcggc aggagcatgc
     3061 gtgtaaaagc tcgagaaaac cgagcggtcg cgtagcgggg aaaaggaaag gccgcaaagc
     3121 ggccgttttt ccataggctc cgcccccctg acaagcatca cagaaacgga cgctcaaatc
     3181 agtggtggcg aaacccgaca ggactataaa gatatcagga gtttccccct gatggctccc
     3241 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     3301 gtttatctca ttccacgcca gacactcagt tccgggtagg cagttcgctc caagctggac
     3361 tgtatgcacg aaccccccgt tcagtccgac tgctgcacct tatccggtaa ctatcatctt
     3421 gagtccaacc cggtaagaca cgcacaagcg ccactggcag cagccactgg taagaaggtg
     3481 tacaagagat tactgataca gagttcttga agtctcagcc caactacggc taaactgaaa
     3541 ggacagaatt tggtttcagc ggtcttgtac accctgttac cacggttcag aagttcccca
     3601 acttactgaa ccctcgaaaa aaccgcctcc ccaggcggtt ttttcgtctc ggcgagtaag
     3661 atacgcttta tcat
//
