LOCUS       NZ_CP117755             6237 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Enterobacter hormaechei strain 2022CK-00700 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP117755
VERSION     NZ_CP117755.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32093416
            Assembly: GCF_028622735.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease Submission Group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622735.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 02:00:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,271
            CDSs (total)                      :: 5,156
            Genes (coding)                    :: 5,050
            CDSs (with protein)               :: 5,050
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 22 of 106
            Pseudo Genes (incomplete)         :: 79 of 106
            Pseudo Genes (internal stop)      :: 15 of 106
            Pseudo Genes (multiple problems)  :: 9 of 106
            Pseudo Genes (short protein)      :: 1 of 106
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117755.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00700"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="2022-11-04"
     gene            complement(join(5359..6237,1..615))
                     /locus_tag="PTC84_RS26275"
                     /old_locus_tag="PTC84_26275"
     CDS             complement(join(5359..6237,1..615))
                     /locus_tag="PTC84_RS26275"
                     /old_locus_tag="PTC84_26275"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_273961873.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELID
                     ASPYAKKYTSGVLSFAEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLE
                     LNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSAL
                     PETKQEAAQAITRGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRN
                     IRLKGAIYEQSFNAGEGLRAEIESAAAEYRRDAESRIQRAREVCQSGTERKREENQRRH
                     PRPRPEAVLSHEPAYERDAAHGQPDVADHRPGLRAADRLERGHSVVAESADTRQLREHP
                     GAEEHAGDAVRAEQRRAALDLRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDRAGKT
                     VAERIRAATAGLLEKAGRVGERLRGMADDVWSYATGERGAERARHGLEQAGAEFERSAA
                     PVVERLNAVELQQVREARHQKALELERSQRQRVYHGPSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(605..928)
                     /locus_tag="PTC84_RS26280"
                     /old_locus_tag="PTC84_26280"
     CDS             complement(605..928)
                     /locus_tag="PTC84_RS26280"
                     /old_locus_tag="PTC84_26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1572..1682
                     /locus_tag="PTC84_RS26285"
                     /old_locus_tag="PTC84_26285"
     CDS             1572..1682
                     /locus_tag="PTC84_RS26285"
                     /old_locus_tag="PTC84_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(1937..2398)
                     /locus_tag="PTC84_RS26290"
                     /old_locus_tag="PTC84_26290"
     CDS             complement(1937..2398)
                     /locus_tag="PTC84_RS26290"
                     /old_locus_tag="PTC84_26290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(2481..3071)
                     /locus_tag="PTC84_RS26295"
                     /old_locus_tag="PTC84_26295"
     CDS             complement(2481..3071)
                     /locus_tag="PTC84_RS26295"
                     /old_locus_tag="PTC84_26295"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_023343079.1"
                     /translation="MPSLSVERWLALATAGDSQARFHLGKIFSCGLGVHQDYKQAIYWF
                     QLAAEQGHDDAQFSLGWAYHLGLGGYKDAQKASEWYQKAAEQGHAEAQFQLGRLYAFSD
                     LRNDALAVEWFLCAAKQDHSSAQFKLGWMYHLGMGVEQSDRQAFHWFILAAEQDHAGAQ
                     ASLGEMYKNGWGVEQDDVAAQDWFYRARTMHYP"
                     /name="tetratricopeptide repeat protein"
     gene            complement(3076..3612)
                     /locus_tag="PTC84_RS26300"
                     /old_locus_tag="PTC84_26300"
     CDS             complement(3076..3612)
                     /locus_tag="PTC84_RS26300"
                     /old_locus_tag="PTC84_26300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023343080.1"
                     /translation="MLDTDCITVLTARGITEILNSGGSQAWRLDATHVAKLPYLVCVQN
                     SKKDWGSQEAKHHHAFMIGKISGVSRAHENPKRWIINIDSYAEIDIPGQWDGNRNPVSY
                     RKLEDMGIDISKVDFQPLPKEDSLVLNTIKGGDEYDEYEEDSNRVRPINLKEAKAGLAL
                     YFGVNEDDIQITIKG"
                     /name="hypothetical protein"
     gene            complement(4275..5189)
                     /gene="lpxO"
                     /locus_tag="PTC84_RS26305"
                     /old_locus_tag="PTC84_26305"
     CDS             complement(4275..5189)
                     /gene="lpxO"
                     /locus_tag="PTC84_RS26305"
                     /old_locus_tag="PTC84_26305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     Typing          complement(1030..1216)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          1196..1682
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.959"
                     /coverage="93.0"
                     /evalue="3.59e-159"
     Typing          complement(2..615)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.585"
                     /coverage="100.0"
                     /evalue="2.9000000000000002e-126"
ORIGIN
        1 taccacctca aaacctgcgc tttccagcgc ctcagtgacg tcctggcggg ttttcagctc
       61 cccggacgag gccaaggcca gtaaaccccg tgtaattgcc tgagcggctt cctgcttcgt
      121 ttccggcagc gcggacggcg tgaccagcgc ccgccggttt tcaggcgcgt tcgggtcatg
      181 cagccccagc ctgccgttca cgatggtctg ccaggcatca atgcgcggac ggtcggcgcg
      241 gtcgtaatac ggctggagac gcttgccagt cagcagctcc gtgttcggga tcaggaagtt
      301 cagctccagc cgccccttgt cccggtgctc aacccacagc acgctgtact ggtctttgtc
      361 gagtccgggc atcagaaccc gttcaaagct cgccatcagc ttttcgcgct gcccgggcgg
      421 taaatccttt tcggcaaagg acaggacgcc ggatgtgtac ttttttgcgt agggtgaggc
      481 gtcgataagc tccctgactt cttccggctt cccctgcaga acggtggcgc cctcgcgctg
      541 tcggtctttc cccagcagat aatccaccgg accggcgcca ccgccgcgcc ctcgcgggtg
      601 aaacttaacg atcatcgtct gcaccctttt ccagcacggc atgccgcagc cgctcgagtc
      661 cggcatctat ggccatcagc gcggcgacga cctgcacgcg gtcgtgcccg ctgccaccac
      721 cggcgttaac ctgccgggca atctggttaa ggttgttccc catgccggca agctgacgaa
      781 gcagcgccgg cgagatcgag ggaagcttgc cggcgcgggc gggtttctcg tccaggcacg
      841 tctggcgcat ccaggctgca agctgtttac cgtcgcagcg ctccagcaga cgccggtgct
      901 catcctcggt cacccacatc gtgagcattt tgctgcgttt gtctgccaat ccggcctcct
      961 gataacgccg ggacgggcgg gaaatctttt cccggtcggc gcggtgttgg acaagccgca
     1021 ggcgcgtcag tggttttagc gggtttcggg gcgcagccct gaaccagtca cgtagcgcta
     1081 gcggagtgta tactggctta atatggtaaa tcgaagtggg aattcaggga agtgcttcat
     1141 gtggcaggta aaaaatgcgt ctgtggggcg taagtgcgaa atgctgggga cgatgaaatg
     1201 acgcctcctc gctcactcgg tcgctacgct cctgccgtga gactgcggcg ggcgttaccg
     1261 gctcacgaat ggcgggataa agagatggcg ctcaaatcag gaagggctgg gaaaaggatg
     1321 cggcgttgcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa atatgacgct
     1381 caaatcagtg gcggcgaaac ccgacaggac tataaagatc ccaggcgttt ccccctggta
     1441 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     1501 gtggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     1561 agctggactg tatgcacgac ccccccgttc agtccgacta ccacgcccat tccggtaact
     1621 atcaacttga gtccaacccg gaaagacacg acaaaatcgc cattggcggt agccactggt
     1681 aagcaggatg tgcgagagat gtatcgagag ttcttgaagt ggtggccgga ctacggctac
     1741 actggaagga cagatttggt gacacgtctc gcacaagact gttaccacgg ttcagaagtt
     1801 ccccaactta ctgaaccttc gaaaaaccac ctccccaggt ggttttttcg ttttcagagc
     1861 aagagattac gcgcagagca aaacgatctc aagaagatca ttttacaaat tggataatat
     1921 ccctccgccc ctaacactaa tataaaatta tcgggcttgc atgcccctca ccattttccc
     1981 catatccacc accaaacaat agttcgtcga gttctttgct agcttgatca atacacgcag
     2041 ccacatgaat agcagaaggg tactcatcat actgaaaatt atcgcttttt tcaggaggga
     2101 gtcgatcata atacggatga caatccacat aataatgacg tattagcatc ccagttttat
     2161 cttcgtactc tgatggctgc aacgtactcc ctgggaaagc ttttgcaaca tgtatagcag
     2221 cttgaaaggt atcaaaatat cgcccaaaca ttggcgtttc aaagctaggc gactctgtcg
     2281 aaaaatcccc cataacttgc gccaaataac cattatctat ctgccattga acatctgatc
     2341 ccatagccca ctctcccaga gttactccta ataaataacc ataatatgta tttaccataa
     2401 cgcttcatgt tatgaatcag cagcagccag ctccgcaagc ctcttacacg taggcgtaaa
     2461 aaaacccgca cagggcgggt ttagggatag tgcatggttc tggcacgata aaaccaatcc
     2521 tgcgcagcaa catcatcttg ctcgaccccc catccatttt tatacatctc accaagagat
     2581 gcctgagctc ctgcatgatc ttgctcagcg gccagaataa accaatggaa agcctgccta
     2641 tcactttgct caacgcccat accgagatgg tacatccagc caagtttaaa ttgggcacta
     2701 ctgtggtctt gcttcgcagc acagagaaac cattcgactg ctaacgcatc atttcttaaa
     2761 tcagagaaag catacagcct tccaagctga aactgagcct ctgcatgacc ttgctcagca
     2821 gctttctgat accattccga agctttttga gcatctttat atccacctaa ccccagatga
     2881 tatgcccaac caagactaaa ctgggcatca tcatggcctt gctcagctgc aagctgaaac
     2941 cagtatatag cctgcttata atcctgatgc acaccaagac cgcatgagaa tatcttaccc
     3001 agatgaaatc tagcctggct atctccagca gtagctagtg ctaaccagcg ctcaacagag
     3061 agtgacggca taaggtcatc ccttaattgt gatctgaata tcatcctcat tcacaccaaa
     3121 atataaagcc aaccctgctt tagcctcttt tagatttatc ggcctaactc tgttgctatc
     3181 ctcctcatac tcatcatatt catccccgcc ttttatcgta ttcaagacta aggaatcctc
     3241 tttaggtagg ggttggaaat ccactttgga gatatcaatt cccatgtctt caagttttct
     3301 ataggagact ggattacgat tgccgtccca ctgcccaggg atatcaattt cagcatagga
     3361 atcaatattt atgatccagc gcttagggtt ttcatgggct cgacttactc cggatatttt
     3421 cccgatcata aatgcatgat gatgcttagc ctcctgacta ccccagtctt ttttcgaatt
     3481 ttggacgcaa accaggtacg gaagcttagc tacgtgagta gcatcaagtc tccaagcctg
     3541 gcttcctcca ctatttaaaa tctcagtaat acccctggcc gtaagaacag taatgcagtc
     3601 agtatcaagc atagatatct ccatttttgt tttaaaaaag gcaagtcacc gcgtaatgac
     3661 aatcttttta gcatcaaaaa cggaagagct caacataaaa ccacaagata tatacaaata
     3721 actatccgaa gtactacgac gattactcct ggggctgcaa aaaaaccagt aaaacactac
     3781 tcgacttcgg ccggagggct ttttctctct ggactcagtc tgagtgacaa catgatgtgt
     3841 gaagtaagaa cccgccacac cgccgcattt aacataagag ttcttggccg caccccctga
     3901 gagcccctca gcgcgtttct gcggcctgag ggccgtttcc ggcacctttc cccggcccaa
     3961 accctgaaaa acgcccctga taatttctgc gcggctgcat ggctatgcac aagagtgaat
     4021 ggcggccggt ttcgtgaagg aaataccgtg aaagctccga aagaaacgct taattgcaca
     4081 gcagcacatg aacgtgatca gatgaaatct gtctactgcc gcacttcttc acgaaaggcc
     4141 cgctgagcgt cagattcggg cctttcttac taaaaagatt tcaatagaat tttttagcag
     4201 gcgattttta tctataacca tacattgagc atagaagacc caaatttatg tatgactcaa
     4261 tagaaaaaaa caaatcacag ccatctaaac actgagacag caagccaaac aaaaatcaaa
     4321 gaaataactc catttttaat caaataatat agttttcgat tccttttttt tagcttctga
     4381 cctgcatcac gaatagcatt aacatattta aataaatgat ttatagcacc agtcctgtca
     4441 ttattatcgt tgggtgatgc tgccgctgac attagcaccc cacctatcca cctatttatt
     4501 ttttgtgacc accgccactt catcggcctt tctatatcac agaacaaaat aatacgtgtc
     4561 tgctcggtat cattctgagc ccagtgaaca aaagtttcgt caaaaattat cacttcacca
     4621 tcatgccaac tgcggcgtac accatcaact tcaataaaac accgttcatc atttggtgtt
     4681 gataatccta aatgataacg aactgaaccg gcataagggt cacgatgctt tcctaaatgg
     4741 cttcctgaag gcaattcagc aaacatcgct gctttaatac agggaataga gctaaccagt
     4801 tgggttgtta ctgggcaaag ccgctccgct gatggatgag catctccata ccacttgagg
     4861 taaaagcgct tccaaccgcg cttaaaaaat gtattaaatc ctgcatcatt atttttttca
     4921 gatgatttaa tgtgcccttg caaattaaca gcttcgtctc ttataacctg ccagttatca
     4981 attaatggtt gaagttcagg aaatgtttct gggggaaacc atgcctgctt tgacggcagc
     5041 ttcgaaaagc ctgtcataaa catatttatc ggagccataa atgttgaatg atcaaataac
     5101 tgcctggaaa acttttgttt tattttaccg cgagaatgcg catacaatac actgataaga
     5161 aaaacaaaaa taataataag tgacagcatg tgtatattcc ttgttcacgc caataataaa
     5221 aatctcttaa agataacata atgaatgcac ttaaattata atctaaatgc tatttatcaa
     5281 tagtataatc gaagcttcac cagaacatat ctactacaca ggcagagagg atttcagcag
     5341 gaattatgct gaaatgaatc aaagggacgg accgtgatat acccgctgtc gctgtgatcg
     5401 ttcgagctcc agcgctttct gatgccgcgc ctcccgcacc tgctgcagct cgacggcatt
     5461 cagccgctcg acaaccggcg cagctgatcg ctcaaactct gcacctgcct gctcaagccc
     5521 gtgacgcgct cgctcagcgc cgcgttctcc cgttgcataa gaccagacat cgtccgccat
     5581 tccgcgaagg cgctctccca ctcgtccagc cttttcgagt agtcctgctg tagctgctct
     5641 aatgcgctca gcaactgttt ttccagctct gtcatgtgct atttccccgc cagtatcatc
     5701 cagctcgaat cctctccgaa ccgtcccgct tccgggttca ccctcacgca gcggcgtctc
     5761 tgctcgccgc aggtcgagag ctgcacgccg ctgttccgct cggacagcat cgcctgcgtg
     5821 ctcttctgct cccggatgct cacgtaattg tcgagtatct gctgactctg ccaccacaga
     5881 atggccgcgt tcgaggcgat cagcagcacg gagaccagga cgatggtcag ccacgtctgg
     5941 ctgaccatgc gcagcatccc tttcgtacgc tggctcatgg ctgaggacag cttccggtcg
     6001 tggtcgagga tggcggcgct gattctcttc tcgctttcgc tcagttccgc tctgacagac
     6061 ttctcgtgct cgctgaatgc ggctttcagc atctcgccgg tactctgctg ctgcgctttc
     6121 gatttctgct ctaagtcctt cgccagcgtt aaaagactgt tcatagatgg ctcccttcag
     6181 tcggatgttt cgccccccgt ccgggtcggc aatgctgatg ctgctttttg tggtgcg
//
