LOCUS       NZ_CP117748             4167 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Klebsiella pneumoniae strain 2022CK-00752 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP117748
VERSION     NZ_CP117748.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32093417
            Assembly: GCF_028622755.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease Submission Group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 67.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622755.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 01:45:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,836
            CDSs (total)                      :: 5,712
            Genes (coding)                    :: 5,568
            CDSs (with protein)               :: 5,568
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 57 of 144
            Pseudo Genes (incomplete)         :: 92 of 144
            Pseudo Genes (internal stop)      :: 25 of 144
            Pseudo Genes (multiple problems)  :: 25 of 144
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117748.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00752"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2022-11-14"
     gene            complement(31..144)
                     /locus_tag="PTC85_RS29110"
                     /old_locus_tag="PTC85_29110"
                     /pseudo=""
     CDS             complement(31..144)
                     /locus_tag="PTC85_RS29110"
                     /old_locus_tag="PTC85_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            560..751
                     /locus_tag="PTC85_RS29115"
                     /old_locus_tag="PTC85_29115"
     CDS             560..751
                     /locus_tag="PTC85_RS29115"
                     /old_locus_tag="PTC85_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            751..1152
                     /locus_tag="PTC85_RS29120"
                     /old_locus_tag="PTC85_29120"
     CDS             751..1152
                     /locus_tag="PTC85_RS29120"
                     /old_locus_tag="PTC85_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1498..1740)
                     /locus_tag="PTC85_RS29125"
                     /old_locus_tag="PTC85_29125"
     CDS             complement(1498..1740)
                     /locus_tag="PTC85_RS29125"
                     /old_locus_tag="PTC85_29125"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(2106..2738)
                     /locus_tag="PTC85_RS29130"
                     /old_locus_tag="PTC85_29130"
     CDS             complement(2106..2738)
                     /locus_tag="PTC85_RS29130"
                     /old_locus_tag="PTC85_29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(2738..3853)
                     /locus_tag="PTC85_RS29135"
                     /old_locus_tag="PTC85_29135"
     CDS             complement(2738..3853)
                     /locus_tag="PTC85_RS29135"
                     /old_locus_tag="PTC85_29135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     Typing          complement(32..439)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 ggcgtataac ttcaagacta actccctaaa tcagttacca gtggctgctg ccagtggcgc
       61 tttgtcgtgt ctttccgggt tggactcaag acgatagtta ccggattagg cgcagcagtc
      121 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact
      181 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
      241 gaaaggcagg aacaggagag cgcacgaggg agccatcagg gggaaacgcc tggtatcttt
      301 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gattccgtga tgctcgtcag
      361 gggggcggag cctatggaaa aacggccgct gtgcggcctt tccttctttc ctccatgcga
      421 ttactcggtt ttctcgagcg tttctacgca tgctcctgcc gctcgccgca gccgaacggc
      481 aggagcgtag cgactgagtg agcgaggaag cggaaaagag gagatcccta cactgaccac
      541 tgacgctctg aggcctctta tgacgaaaca acagaaaaca gcgctgaaca tggctaaatt
      601 catccagaat cagtctctgc tcctgctgga gaagcttaac gagcttgatc tcgatgtcga
      661 ggccgacctt tgtgagaaac tccacgatga tgcggagcat cttttccgta cactgtcctc
      721 gagactggat gcattacagg atggtaactg atggccagag tgtctattag tgaggctgcc
      781 cgactgacgg gtaagagtag aacaacactg cacaggttaa ttaaagcagg tgacctgtcc
      841 acctgtagcg gtgagcgcaa tgcaaaaatg gtagatactt cagagctttt acgtgtcttt
      901 ggcccctttg agcaaccgaa gggtgaacac cattctggac aggtctctgg acagcgtgat
      961 acagcctctg ctgaccagag tgaacaggtg atccgtcatc tgaaacagga gattgaacac
     1021 ctgaacacct tacttttgtc caaggattcg cacattgaca gcctgaaaca ggccatgctc
     1081 cttcttgaaa atcaccaggc aaaattaact gaatccccgg cgccatggtg gaaattctgg
     1141 aaaaaatcct gaccccgtca tgcttcagtt ttcctctccg gccatcccag ggagttcctg
     1201 aaccttccca aaaatcgtca gatctctgaa cgctcctcac cactaaaaat acccacaccc
     1261 cacgcaaaat taaaattttg cagattcctt atttacaaca atgggttaat gaaaaataaa
     1321 tgtgcggtga tgtgttacaa agtgcagatc caagtcgtgg acgactctat ggcgaatcgc
     1381 tgacgaattg cccacgattc atatgtgaat cacgctttat tatcatattg ttatgatgaa
     1441 tcgactcttg attcaattca tcactttggg tgttttttgc tcatggtgac tcttgattca
     1501 attcacgatt cgaatcgccg gtgaatcgtg tggtgagtcg tccggcgact tgccataacg
     1561 tgattgccgg ccgtcgacga acccgattcc tgaaggtgct gaaaaccggt tttccggctg
     1621 cgccggcaac gcttcacggc cacgcaataa attgcgtccc cgttcaccgt tttcggagga
     1681 gaagaacgcc ggaaacagga cttcccctgg ctgaatggtg tgaatttccg cgctatgcac
     1741 ttgcgcgcat actcatgcat gccgtaaaaa cagagcctgc gcgtttctgg cgggttttcg
     1801 ggtggtttgt tgcctgtttt accggtttcc cgtcagaaac gtcctgagtc cgttttagcg
     1861 gtgcgtacaa ttatgatttt atgttaaatt gttttaagtg aatagagaga atataactta
     1921 agggagcatc ttgtgctggg tcttttacat tagatctgca caatcattgt tttggcatct
     1981 ggtgatgtct ttaggacgtt tgagattgct gataactaaa gtgtatgatg ctttctatac
     2041 gttatccggc gtttaataac accctggtga taagccagcc cgtgaagggc tggcttgatt
     2101 tggatttata tttttgaaat aaaatctttt atttcatcaa agcaattgtc tgctgtgttt
     2161 attattccat ttttcatgga tattcctatt cttgagtcgg attctctatt gcagctaaga
     2221 tagacttgta ccttagcttt tttttcgttt gttggataag tgtactcttt gtcactcgct
     2281 gagatgtcat ctttattatt tttaattttt tcgaaaaatt ctattatgta ttcatataac
     2341 gctcgagtgc ttgcgtgttc aattatcaga tcttcaatgg caccttgctt gtcttttcct
     2401 ggcattataa agaaagaaat ttttgtattg ccattcatag ctatctcgtt atgcgagctg
     2461 ggggaggaga tttgtagttc tcgcaatgtg gcaattattg atctttctgt gtcagaaaat
     2521 gatgtgtcag catctaatat aataaaaata ctttcttttt catcgaaaga atcaatgttt
     2581 ttgaatgctc taagtgcttg ttttaatttt gattttccat ttattttttc gacaaaaaca
     2641 tcggtaatgc cttgtatttc aagtaggttg catagaaaat gaaaatcatc atcaccctca
     2701 acgaaaatga tctttttgct attatttatg tctttcatta tcttacctcc cagtcgttct
     2761 caactgagta ggagaaatca tccatattga attgtacggc tgttggtttt agggagttct
     2821 tttctcttcc tagtctaata taagcgatat cttcattttt gctatctatt tcgtttattg
     2881 ctcgtatcac atcttgacta tgagttgttg caaatatttg attgttgttt tgttttgcag
     2941 atgaaattaa tgattttatt atgtctttaa ttactgagta atgtattccg ttctcgattt
     3001 catcaataag aataattgag ttttcctgta ccagcactga gctgataaaa gataatgctc
     3061 gactgatacc ctcgccaagc attgatattt catttagctc actaaatcca atgtctaaat
     3121 atatttcctt attgttacct atcgcagata ttgctatatc tacaattctg tcatctattt
     3181 tttttaagtt ttctagtatg tcttttttcc ttttttgtgt tagtaggcta gatacatttg
     3241 taattgttgc ctctttgttt atctttctgg atgttgttat tattgttgct gtcttaaatt
     3301 tataattaat cccatccctt tcgtttttta tttcggattt tatttctacg ccatcttctt
     3361 tcaattgcat agagaaaagc ttattggaaa ccctgttttt cgcattgggt gtctcggtca
     3421 catttatttt taaagagttt actgtggcct gagaaccgat agtttgacga ggtatgtttt
     3481 ggttagatag tacggtgtca acgtttagag acaaacttga ttctaatctc tgatttgttt
     3541 ctgtttcata tgtttgtgtt acttcagagc gttcatctcg aatgactatg gacatctttt
     3601 gtgatgtgtc catatcatga aagtaactaa cccaaaatgg cttgtttttg tttatttctg
     3661 ccatttcttt tctgaatgct tgaatgttta acaaaacagc cgggtttttt acatcataaa
     3721 aacaaaaaat agcatccagt atagatgttt tcccgtagtt gtttttccca gctatgatat
     3781 ttaaagtctt taattgcttt agatatagct cattgaagca tttgaagttc tttaaactta
     3841 tttcctttat catgatagcc tctgttcgtg tttttgttat atgccatacg atttgttagt
     3901 accttaataa aatgtacagc tttcattgtg attagctaga ctttatgtca aagattaact
     3961 ttgattgctt gtgttttaat cgattttttt tactttcttc ttgagattat ctggcagcag
     4021 ccgtaagctt ccggctgtga acgaaaaaaa ccgccttggc gggcggtttt ttcgaaaggt
     4081 tcagtagagt tggcgctcta tgaaccgagg taactggctt aaagggagcg cagtcaccaa
     4141 aactgttcgt ttagtttagc tttaact
//
