LOCUS       NZ_CP117744             4167 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Klebsiella pneumoniae strain 2022CK-00768 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP117744
VERSION     NZ_CP117744.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32733973
            Assembly: GCF_028622155.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease Submission Group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 82.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622155.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 01:43:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,833
            CDSs (total)                      :: 5,709
            Genes (coding)                    :: 5,566
            CDSs (with protein)               :: 5,566
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 56 of 143
            Pseudo Genes (incomplete)         :: 91 of 143
            Pseudo Genes (internal stop)      :: 26 of 143
            Pseudo Genes (multiple problems)  :: 25 of 143
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117744.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00768"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2022-11-05"
     gene            411..524
                     /locus_tag="PTC88_RS29085"
                     /old_locus_tag="PTC88_29085"
                     /pseudo=""
     CDS             411..524
                     /locus_tag="PTC88_RS29085"
                     /old_locus_tag="PTC88_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            869..1984
                     /locus_tag="PTC88_RS29090"
                     /old_locus_tag="PTC88_29090"
     CDS             869..1984
                     /locus_tag="PTC88_RS29090"
                     /old_locus_tag="PTC88_29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            1984..2616
                     /locus_tag="PTC88_RS29095"
                     /old_locus_tag="PTC88_29095"
     CDS             1984..2616
                     /locus_tag="PTC88_RS29095"
                     /old_locus_tag="PTC88_29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            2982..3224
                     /locus_tag="PTC88_RS29100"
                     /old_locus_tag="PTC88_29100"
     CDS             2982..3224
                     /locus_tag="PTC88_RS29100"
                     /old_locus_tag="PTC88_29100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(3570..3971)
                     /locus_tag="PTC88_RS29105"
                     /old_locus_tag="PTC88_29105"
     CDS             complement(3570..3971)
                     /locus_tag="PTC88_RS29105"
                     /old_locus_tag="PTC88_29105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3971..4162)
                     /locus_tag="PTC88_RS29110"
                     /old_locus_tag="PTC88_29110"
     CDS             complement(3971..4162)
                     /locus_tag="PTC88_RS29110"
                     /old_locus_tag="PTC88_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          117..524
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 gcctcagagc gtcagtggtc agtgtaggga tctcctcttt tccgcttcct cgctcactca
       61 gtcgctacgc tcctgccgtt cggctgcggc gagcggcagg agcatgcgta gaaacgctcg
      121 agaaaaccga gtaatcgcat ggaggaaaga aggaaaggcc gcacagcggc cgtttttcca
      181 taggctccgc ccccctgacg agcatcacgg aatctgacgc tcaaatcagt ggtggcgaaa
      241 cccgacagga ctataaagat accaggcgtt tccccctgat ggctccctcg tgcgctctcc
      301 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
      361 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
      421 cccccgttca gtccgactgc tgcgcctaat ccggtaacta tcgtcttgag tccaacccgg
      481 aaagacacga caaagcgcca ctggcagcag ccactggtaa ctgatttagg gagttagtct
      541 tgaagttata cgccagttaa agctaaacta aacgaacagt tttggtgact gcgctccctt
      601 taagccagtt acctcggttc atagagcgcc aactctactg aacctttcga aaaaaccgcc
      661 cgccaaggcg gtttttttcg ttcacagccg gaagcttacg gctgctgcca gataatctca
      721 agaagaaagt aaaaaaaatc gattaaaaca caagcaatca aagttaatct ttgacataaa
      781 gtctagctaa tcacaatgaa agctgtacat tttattaagg tactaacaaa tcgtatggca
      841 tataacaaaa acacgaacag aggctatcat gataaaggaa ataagtttaa agaacttcaa
      901 atgcttcaat gagctatatc taaagcaatt aaagacttta aatatcatag ctgggaaaaa
      961 caactacggg aaaacatcta tactggatgc tattttttgt ttttatgatg taaaaaaccc
     1021 ggctgttttg ttaaacattc aagcattcag aaaagaaatg gcagaaataa acaaaaacaa
     1081 gccattttgg gttagttact ttcatgatat ggacacatca caaaagatgt ccatagtcat
     1141 tcgagatgaa cgctctgaag taacacaaac atatgaaaca gaaacaaatc agagattaga
     1201 atcaagtttg tctctaaacg ttgacaccgt actatctaac caaaacatac ctcgtcaaac
     1261 tatcggttct caggccacag taaactcttt aaaaataaat gtgaccgaga cacccaatgc
     1321 gaaaaacagg gtttccaata agcttttctc tatgcaattg aaagaagatg gcgtagaaat
     1381 aaaatccgaa ataaaaaacg aaagggatgg gattaattat aaatttaaga cagcaacaat
     1441 aataacaaca tccagaaaga taaacaaaga ggcaacaatt acaaatgtat ctagcctact
     1501 aacacaaaaa aggaaaaaag acatactaga aaacttaaaa aaaatagatg acagaattgt
     1561 agatatagca atatctgcga taggtaacaa taaggaaata tatttagaca ttggatttag
     1621 tgagctaaat gaaatatcaa tgcttggcga gggtatcagt cgagcattat cttttatcag
     1681 ctcagtgctg gtacaggaaa actcaattat tcttattgat gaaatcgaga acggaataca
     1741 ttactcagta attaaagaca taataaaatc attaatttca tctgcaaaac aaaacaacaa
     1801 tcaaatattt gcaacaactc atagtcaaga tgtgatacga gcaataaacg aaatagatag
     1861 caaaaatgaa gatatcgctt atattagact aggaagagaa aagaactccc taaaaccaac
     1921 agccgtacaa ttcaatatgg atgatttctc ctactcagtt gagaacgact gggaggtaag
     1981 ataatgaaag acataaataa tagcaaaaag atcattttcg ttgagggtga tgatgatttt
     2041 cattttctat gcaacctact tgaaatacaa ggcattaccg atgtttttgt cgaaaaaata
     2101 aatggaaaat caaaattaaa acaagcactt agagcattca aaaacattga ttctttcgat
     2161 gaaaaagaaa gtatttttat tatattagat gctgacacat cattttctga cacagaaaga
     2221 tcaataattg ccacattgcg agaactacaa atctcctccc ccagctcgca taacgagata
     2281 gctatgaatg gcaatacaaa aatttctttc tttataatgc caggaaaaga caagcaaggt
     2341 gccattgaag atctgataat tgaacacgca agcactcgag cgttatatga atacataata
     2401 gaatttttcg aaaaaattaa aaataataaa gatgacatct cagcgagtga caaagagtac
     2461 acttatccaa caaacgaaaa aaaagctaag gtacaagtct atcttagctg caatagagaa
     2521 tccgactcaa gaataggaat atccatgaaa aatggaataa taaacacagc agacaattgc
     2581 tttgatgaaa taaaagattt tatttcaaaa atataaatcc aaatcaagcc agcccttcac
     2641 gggctggctt atcaccaggg tgttattaaa cgccggataa cgtatagaaa gcatcataca
     2701 ctttagttat cagcaatctc aaacgtccta aagacatcac cagatgccaa aacaatgatt
     2761 gtgcagatct aatgtaaaag acccagcaca agatgctccc ttaagttata ttctctctat
     2821 tcacttaaaa caatttaaca taaaatcata attgtacgca ccgctaaaac ggactcagga
     2881 cgtttctgac gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg
     2941 cgcaggctct gtttttacgg catgcatgag tatgcgcgca agtgcatagc gcggaaattc
     3001 acaccattca gccaggggaa gtcctgtttc cggcgttctt ctcctccgaa aacggtgaac
     3061 ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aaccggtttt
     3121 cagcaccttc aggaatcggg ttcgtcgacg gccggcaatc acgttatggc aagtcgccgg
     3181 acgactcacc acacgattca ccggcgattc gaatcgtgaa ttgaatcaag agtcaccatg
     3241 agcaaaaaac acccaaagtg atgaattgaa tcaagagtcg attcatcata acaatatgat
     3301 aataaagcgt gattcacata tgaatcgtgg gcaattcgtc agcgattcgc catagagtcg
     3361 tccacgactt ggatctgcac tttgtaacac atcaccgcac atttattttt cattaaccca
     3421 ttgttgtaaa taaggaatct gcaaaatttt aattttgcgt ggggtgtggg tatttttagt
     3481 ggtgaggagc gttcagagat ctgacgattt ttgggaaggt tcaggaactc cctgggatgg
     3541 ccggagagga aaactgaagc atgacggggt caggattttt tccagaattt ccaccatggc
     3601 gccggggatt cagttaattt tgcctggtga ttttcaagaa ggagcatggc ctgtttcagg
     3661 ctgtcaatgt gcgaatcctt ggacaaaagt aaggtgttca ggtgttcaat ctcctgtttc
     3721 agatgacgga tcacctgttc actctggtca gcagaggctg tatcacgctg tccagagacc
     3781 tgtccagaat ggtgttcacc cttcggttgc tcaaaggggc caaagacacg taaaagctct
     3841 gaagtatcta ccatttttgc attgcgctca ccgctacagg tggacaggtc acctgcttta
     3901 attaacctgt gcagtgttgt tctactctta cccgtcagtc gggcagcctc actaatagac
     3961 actctggcca tcagttacca tcctgtaatg catccagtct cgaggacagt gtacggaaaa
     4021 gatgctccgc atcatcgtgg agtttctcac aaaggtcggc ctcgacatcg agatcaagct
     4081 cgttaagctt ctccagcagg agcagagact gattctggat gaatttagcc atgttcagcg
     4141 ctgttttctg ttgtttcgtc ataagag
//
